FastQCFastQC Report
Fri 27 May 2016
SRR2139071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10509270
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140850.047.889051
GTATCAA258200.036.583271
TCAACGC317000.029.4837254
ATCAACG317650.029.3025263
CAACGCA324100.028.8246675
TATCAAC331650.028.3525922
AACGCAG333150.028.1060686
CTTATAC77650.024.4500481
ACGCAGA384550.024.3049627
CGCAGAG388450.024.0499638
GCAGAGT450800.020.5817769
GTACATG291600.020.4980141
TACATGG290650.019.7283212
GAGTACT280800.019.62058612-13
TATACAC132850.019.1374443
AGAGTAC408850.019.0838710-11
ACATGGG297950.018.8556543
CAGAGTA440400.018.07027410-11
GTGGTAT76200.017.8606361
AGTACTT306500.017.72487412-13
Produced by FastQC (version 0.11.5)