##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139071_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10509270
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.564560335779746	34.0	31.0	34.0	31.0	34.0
2	32.70315559501278	34.0	31.0	34.0	31.0	34.0
3	32.69001985865812	34.0	31.0	34.0	31.0	34.0
4	36.21668403228769	37.0	35.0	37.0	35.0	37.0
5	36.17402521773634	37.0	35.0	37.0	35.0	37.0
6	36.216285622122186	37.0	37.0	37.0	35.0	37.0
7	36.174846302359725	37.0	37.0	37.0	35.0	37.0
8	36.15141004084965	37.0	37.0	37.0	35.0	37.0
9	37.87872535390184	39.0	38.0	39.0	35.0	39.0
10-11	37.80979582787387	39.0	38.0	39.0	35.0	39.0
12-13	37.78150751669716	39.0	38.0	39.0	35.0	39.0
14-15	39.1243679627605	40.0	39.0	41.0	36.0	41.0
16-17	39.09623646552044	40.0	38.0	41.0	36.0	41.0
18-19	39.057005196364734	40.0	38.5	41.0	36.0	41.0
20-21	39.0414577390466	40.0	39.0	41.0	35.5	41.0
22-23	38.926891188387714	40.0	38.0	41.0	35.0	41.0
24-25	38.78949060499268	40.0	38.0	41.0	35.0	41.0
26-27	38.73972186077315	40.0	38.0	41.0	34.5	41.0
28-29	38.683747194955146	40.0	38.0	41.0	35.0	41.0
30-31	38.610291574177126	40.0	38.0	41.0	34.5	41.0
32-33	38.49596973985983	40.0	38.0	41.0	34.0	41.0
34-35	38.30463523786618	40.0	38.0	41.0	34.0	41.0
36-37	38.438669199344375	40.0	38.0	41.0	34.0	41.0
38-39	38.417230763529474	40.0	38.0	41.0	34.0	41.0
40-41	38.38932978759735	40.0	38.0	41.0	34.0	41.0
42-43	38.424927450903674	40.0	38.0	41.0	34.0	41.0
44-45	38.36480134093499	40.0	38.0	41.0	34.0	41.0
46-47	38.30889609513916	40.0	38.0	41.0	33.5	41.0
48-49	38.155189541543606	40.0	38.0	41.0	33.0	41.0
50-51	38.1561586493588	40.0	37.5	41.0	33.0	41.0
52-53	38.068395792366324	40.0	37.0	41.0	33.0	41.0
54-55	37.919513558892305	40.0	37.0	41.0	33.0	41.0
56-57	37.7560054991423	40.0	36.5	41.0	33.0	41.0
58-59	37.596367871000474	39.0	36.0	41.0	33.0	41.0
60-61	37.413597901959506	39.0	36.0	41.0	32.0	41.0
62-63	37.20575777693924	39.0	35.0	40.5	32.0	41.0
64-65	36.917701278584374	38.5	35.0	40.0	31.5	41.0
66-67	36.56435850496406	38.0	35.0	40.0	31.0	41.0
68-69	36.23450254783846	37.0	35.0	40.0	31.0	41.0
70-71	35.93436024882891	37.0	34.5	39.5	31.0	41.0
72-73	35.61126004364637	36.0	34.0	39.0	30.0	40.5
74-75	35.246697542023284	36.0	34.0	39.0	30.0	40.0
76-77	34.288804482623554	35.0	33.0	37.0	29.0	39.0
78-79	34.57022832116227	35.0	34.0	37.0	30.0	39.0
80-81	34.37298910173749	35.0	34.0	37.0	29.5	39.0
82-83	34.093496809996694	35.0	34.0	36.0	29.0	38.5
84-85	33.842262784905216	35.0	33.0	36.0	29.0	37.0
86-87	33.5949350045466	35.0	33.0	35.5	29.0	37.0
88-89	33.35259521082142	35.0	33.0	35.0	29.0	36.5
90-91	33.16364992451784	35.0	33.0	35.0	29.0	36.0
92-93	33.07041234636438	35.0	33.0	35.0	29.0	36.0
94-95	32.98973363728747	34.0	32.5	35.0	29.0	36.0
96-97	32.96939269872367	34.0	32.0	35.0	29.0	35.0
98-99	33.09199641468996	34.0	32.5	35.0	29.0	35.0
100-101	31.239774779331977	32.5	29.0	34.5	24.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	3.0
19	5.0
20	50.0
21	333.0
22	1403.0
23	4633.0
24	12055.0
25	25246.0
26	45071.0
27	71381.0
28	103386.0
29	139498.0
30	181556.0
31	230002.0
32	291423.0
33	381240.0
34	528288.0
35	761957.0
36	1257114.0
37	2310740.0
38	2791314.0
39	1299891.0
40	72678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.277039059519744	21.6828401670944	15.872074451610288	24.16804632177557
2	13.400185941728044	21.854757792769476	41.738341026341615	23.00671523916086
3	16.759719443854753	26.87408610437129	31.498860672421653	24.8673337793523
4	11.781294612437545	19.156703787259637	39.30808265205327	29.75391894824955
5	12.675258383813375	38.20122027516852	36.007480634476096	13.116040706542007
6	29.048195454888443	39.069548985757244	18.79258205304289	13.089673506311428
7	25.660937977703302	33.26890766617545	23.369265740557214	17.700888615564033
8	23.98131364024333	37.32255427827052	21.199388730140154	17.496743351346
9	25.0105192844032	17.13813614075954	21.6267923461858	36.22455222865147
10-11	23.57471608126055	27.392664391022127	29.205352005379638	19.82726752233769
12-13	24.523586703275843	24.81868038543353	29.418857087667433	21.238875823623196
14-15	21.65607868351292	26.288592106699237	27.12297681630862	24.932352393479224
16-17	21.08571828498058	29.53350155943858	27.75160277019742	21.629177385383418
18-19	21.095101857227178	28.492348040572562	29.789065252778286	20.62348484942197
20-21	21.841551587415438	27.548095060083767	29.74426045371692	20.86609289878388
22-23	21.556476477040416	27.58526443570915	29.58173876582902	21.276520321421412
24-25	21.3956735173814	27.76797008451	29.651096375825432	21.185260022283163
26-27	21.4131268858135	27.871572344522683	29.60776126811812	21.1075395015457
28-29	21.254089705108115	27.8564091443796	29.659213829113227	21.23028732139906
30-31	21.55592760115181	27.811905880119408	29.514325477889123	21.11784104083966
32-33	21.389276561507867	27.803219775429298	29.48650776249106	21.320995900571777
34-35	21.38028984803195	28.008056715946907	29.57678719562239	21.03486624039876
36-37	21.48018608307712	27.90287040095602	29.398972142592594	21.21797137337427
38-39	21.365114376915074	27.93328637766532	29.405442842117345	21.29615640330226
40-41	21.534678989408516	27.81078950555857	29.302889213818133	21.35164229121478
42-43	21.536861075058265	27.939171823426484	29.073214786484964	21.45075231503029
44-45	21.546418631455484	28.099759546914388	28.886925092344466	21.466896729285658
46-47	21.71488842689728	28.027077196190735	28.51083802253932	21.747196354372665
48-49	21.692977745417117	28.193406633246905	28.3575245203745	21.75609110096148
50-51	21.593245036030666	28.249935250697828	28.38132734698834	21.77549236628317
52-53	21.729654006089298	28.2518095008004	28.333350636446873	21.685185856663427
54-55	21.70617657022659	28.205055278828656	28.254054618033148	21.83471353291161
56-57	21.82862715136983	28.333915856808407	28.090777882058916	21.746679109762848
58-59	21.624965248600002	28.511617510231858	28.277631298476795	21.585785942691345
60-61	21.855098280507292	28.542741897894558	28.041998715403544	21.560161106194613
62-63	21.720477292152427	28.67573235254442	27.991567035297845	21.61222332000531
64-65	21.686186738917552	28.56207968607553	28.047256608501986	21.70447696650493
66-67	21.784396616146207	28.48573108572926	27.940035262622096	21.789837035502437
68-69	21.739005614522885	28.411602104355758	28.063765343114955	21.7856269380064
70-71	21.728451659060756	28.181619398890025	28.136870254988988	21.953058687060228
72-73	21.812565448787836	28.213389542204254	28.08768970044887	21.88635530855904
74-75	21.693583506287613	28.32406296123543	28.051017539256375	21.931335993220582
76-77	21.771352342880114	28.23306147895283	28.017801020787235	21.977785157379813
78-79	21.72179935684318	28.244178411747743	28.012084955989963	22.021937275419113
80-81	21.731253019285475	28.254467605380384	28.051147574474143	21.963131800859994
82-83	21.746972510720337	28.23378703816472	28.13055296517191	21.88868748594303
84-85	21.93578677418478	28.156982512130252	28.032118429886953	21.875112283798014
86-87	21.763296116452356	28.141031802673204	28.145072316694637	21.950599764179803
88-89	21.78745891003887	28.130687767565405	28.112133197533517	21.969720124862214
90-91	21.81407929133389	28.121042931238822	28.154915064311993	21.9099627131153
92-93	21.84302351720439	27.986526518068803	28.242946538979268	21.927503425747535
94-95	21.651821588930364	28.093032256346834	28.277309710216592	21.977836444506206
96-97	21.587949257452767	28.153222959080153	28.35272577967859	21.90610200378849
98-99	21.795173127334177	28.669790034570568	28.328965536773932	21.20607130132132
100-101	20.33376011607607	32.26410453471848	26.225937569359097	21.17619777984636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2159.0
1	1971.5
2	2781.5
3	3995.5
4	5293.0
5	7925.5
6	10289.0
7	12934.0
8	15279.5
9	15731.5
10	14552.0
11	13381.0
12	14935.5
13	20351.5
14	33719.5
15	52054.0
16	66637.5
17	74240.5
18	74707.5
19	70421.0
20	64145.5
21	58042.0
22	55112.0
23	58777.5
24	68380.5
25	83684.5
26	103573.0
27	124596.0
28	148109.5
29	173990.5
30	199983.0
31	227102.0
32	253202.5
33	278432.0
34	302770.0
35	322531.0
36	344322.5
37	366636.0
38	382580.5
39	394050.5
40	404732.5
41	415112.5
42	418989.0
43	421854.0
44	425309.5
45	428694.5
46	434479.0
47	436137.5
48	427992.5
49	414465.5
50	400343.5
51	381898.0
52	359226.5
53	336119.5
54	314218.0
55	288989.5
56	262766.5
57	235886.5
58	206100.0
59	176190.5
60	146432.5
61	118278.0
62	92682.0
63	70339.5
64	52031.5
65	38671.5
66	29512.5
67	22681.0
68	17228.0
69	12164.0
70	8236.5
71	5865.0
72	4054.0
73	2655.5
74	1762.0
75	1136.5
76	687.5
77	412.5
78	268.0
79	179.5
80	121.5
81	88.0
82	67.5
83	42.5
84	24.5
85	15.0
86	12.5
87	12.0
88	9.0
89	4.5
90	2.5
91	3.0
92	2.5
93	2.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.515408777203364E-6
2	0.01595734051937004
3	9.515408777203364E-6
4	8.563867899483029E-5
5	9.515408777203364E-6
6	9.515408777203364E-6
7	9.515408777203364E-6
8	0.0
9	0.0
10-11	1.2370031410364373E-4
12-13	6.185015705182186E-4
14-15	2.7594685453889755E-4
16-17	0.001898324051052071
18-19	9.515408777203364E-6
20-21	4.757847450850009E-6
22-23	1.712965588805275E-4
24-25	0.009718429216282453
26-27	0.019991661491798825
28-29	0.3484030241805533
30-31	0.3246784678816019
32-33	0.3184597720790206
34-35	0.1853238622895839
36-37	0.043942202943131864
38-39	0.03582073441645717
40-41	0.01467465163637206
42-43	0.013461255098425875
44-45	0.016840292769156686
46-47	0.01158281628845263
48-49	0.006792255761006048
50-51	0.0037462324475304102
52-53	0.0014166190795167212
54-55	5.667638546454748E-5
56-57	1.506300074089187E-4
58-59	0.0010057963730668476
60-61	0.001072775561559693
62-63	3.199141776899317E-4
64-65	9.688751547845342E-5
66-67	0.0032532649342541273
68-69	0.005664414667710312
70-71	0.006834432537899845
72-73	0.010388775363190408
74-75	0.007454899558409167
76-77	0.005882840173371367
78-79	0.0038154858818627143
80-81	0.004855220430814427
82-83	0.0017779247528906833
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002334443957041475
92-93	0.014797396014865143
94-95	0.001298717743503895
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	194.0
20-21	671.0
22-23	1519.0
24-25	3899.0
26-27	5556.0
28-29	8649.0
30-31	19316.0
32-33	25561.0
34-35	37702.0
36-37	51764.0
38-39	62105.0
40-41	66655.0
42-43	80758.0
44-45	96694.0
46-47	96279.0
48-49	83260.0
50-51	74134.0
52-53	71660.0
54-55	76371.0
56-57	81123.0
58-59	83154.0
60-61	83669.0
62-63	86831.0
64-65	94630.0
66-67	101419.0
68-69	94268.0
70-71	90657.0
72-73	88682.0
74-75	90847.0
76-77	91449.0
78-79	97355.0
80-81	100474.0
82-83	100973.0
84-85	104564.0
86-87	112751.0
88-89	122206.0
90-91	133812.0
92-93	145930.0
94-95	175591.0
96-97	272558.0
98-99	1012335.0
100-101	6281245.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.55161046977221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.96032136794403	74.32778118200213
2	7.487934929218151	12.51258044858073
3	1.9232440160501332	4.820704046020231
4	0.7399894237225222	2.473092323304616
5	0.3317163246412081	1.3857716571443361
6	0.18463930231287787	0.925614663855365
7	0.10180080470038066	0.5953934825884892
8	0.06998965578977873	0.46781987659688234
9	0.0469360096465502	0.352942127549463
>10	0.15074808476998927	1.9268467515056131
>50	0.0020928426188225957	0.11421977055838398
>100	5.675688468425323E-4	0.08535161107540078
>500	1.9669738662640037E-5	0.011882059218321948
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9030817554406727E-5	1.9030817554406727E-5
2	0.0	9.515408777203364E-6	0.0	1.9030817554406727E-5	1.9030817554406727E-5
3	0.0	9.515408777203364E-6	0.0	2.8546226331610094E-5	1.9030817554406727E-5
4	0.0	3.8061635108813455E-5	0.0	3.8061635108813455E-5	1.9030817554406727E-5
5	0.0	3.8061635108813455E-5	0.0	3.8061635108813455E-5	1.9030817554406727E-5
6	9.515408777203364E-6	3.8061635108813455E-5	1.9030817554406727E-5	3.8061635108813455E-5	1.9030817554406727E-5
7	9.515408777203364E-6	3.8061635108813455E-5	1.9030817554406727E-5	3.8061635108813455E-5	1.9030817554406727E-5
8	9.515408777203364E-6	4.757704388601682E-5	2.8546226331610094E-5	3.8061635108813455E-5	1.9030817554406727E-5
9	9.515408777203364E-6	8.563867899483027E-5	2.8546226331610094E-5	5.709245266322019E-5	1.9030817554406727E-5
10-11	9.515408777203364E-6	8.563867899483027E-5	2.8546226331610094E-5	1.3797342726944877E-4	1.9030817554406727E-5
12-13	9.515408777203364E-6	8.563867899483027E-5	2.8546226331610094E-5	1.7127735798966055E-4	1.9030817554406727E-5
14-15	1.9030817554406727E-5	1.3797342726944877E-4	3.8061635108813455E-5	1.7127735798966055E-4	1.9030817554406727E-5
16-17	2.8546226331610094E-5	1.4273113165805046E-4	3.8061635108813455E-5	2.188544018756774E-4	1.9030817554406727E-5
18-19	2.8546226331610094E-5	1.570042448238555E-4	3.8061635108813455E-5	2.5215833259588914E-4	1.9030817554406727E-5
20-21	2.8546226331610094E-5	1.7127735798966055E-4	3.8061635108813455E-5	2.949776720933043E-4	1.9030817554406727E-5
22-23	6.660786144042355E-5	1.7603506237826223E-4	4.757704388601682E-5	3.2352389842491436E-4	4.2819339497415137E-5
24-25	7.612327021762691E-5	1.9030817554406727E-4	4.757704388601682E-5	3.5207012475652446E-4	7.612327021762691E-5
26-27	8.563867899483027E-5	1.9982358432127064E-4	5.23347482746185E-5	3.663432379223295E-4	7.612327021762691E-5
28-29	8.563867899483027E-5	1.9982358432127064E-4	5.709245266322019E-5	5.661668222436001E-4	7.612327021762691E-5
30-31	9.515408777203364E-5	2.569160369844908E-4	5.709245266322019E-5	0.0011323336444872003	7.612327021762691E-5
32-33	1.04669496549237E-4	2.569160369844908E-4	5.709245266322019E-5	0.0023407905591920275	7.612327021762691E-5
34-35	1.2370031410364373E-4	3.0449308087050764E-4	6.660786144042355E-5	0.003868013667933168	8.563867899483027E-5
36-37	1.4273113165805046E-4	3.5682782914512614E-4	7.612327021762691E-5	0.006275412088565619	8.563867899483027E-5
38-39	1.4273113165805046E-4	3.615855335337278E-4	7.612327021762691E-5	0.010419372611037685	8.563867899483027E-5
40-41	1.6176194921245718E-4	3.7585864669953287E-4	7.612327021762691E-5	0.01482024917049424	8.563867899483027E-5
42-43	1.6176194921245718E-4	3.8537405547673623E-4	7.612327021762691E-5	0.01860738186382118	8.563867899483027E-5
44-45	1.7127735798966055E-4	4.2819339497415137E-4	7.612327021762691E-5	0.023269932164650828	8.563867899483027E-5
46-47	1.807927667668639E-4	4.662550300829649E-4	7.612327021762691E-5	0.028760323029097166	9.039638338343195E-5
48-49	1.9030817554406727E-4	4.7577043886016824E-4	7.612327021762691E-5	0.034169832918937285	9.515408777203364E-5
50-51	1.9030817554406727E-4	5.851976397980069E-4	7.612327021762691E-5	0.03928436513668409	9.515408777203364E-5
52-53	1.9982358432127064E-4	5.899553441866085E-4	7.612327021762691E-5	0.04489845631523408	9.991179216063532E-5
54-55	1.9982358432127064E-4	5.899553441866085E-4	8.563867899483027E-5	0.05047924356306385	1.0942720093783868E-4
56-57	1.9982358432127064E-4	5.994707529638119E-4	9.515408777203364E-5	0.05621703505571747	1.1418490532644038E-4
58-59	1.9982358432127064E-4	5.994707529638119E-4	9.515408777203364E-5	0.06184064164304466	1.1418490532644038E-4
60-61	2.09338993098474E-4	6.28016979295422E-4	9.515408777203364E-5	0.06693614304323706	1.1418490532644038E-4
62-63	2.09338993098474E-4	6.42290092461227E-4	9.515408777203364E-5	0.07199358280832066	1.2370031410364373E-4
64-65	2.09338993098474E-4	7.422018846218624E-4	1.04669496549237E-4	0.07756961235176182	1.2370031410364373E-4
66-67	2.09338993098474E-4	7.46959589010464E-4	1.04669496549237E-4	0.08304097239865377	1.284580184922454E-4
68-69	2.188544018756774E-4	7.517172933990657E-4	1.04669496549237E-4	0.08862175964648353	1.332157228808471E-4
70-71	2.188544018756774E-4	7.564749977876674E-4	1.04669496549237E-4	0.09324624831220438	1.4273113165805046E-4
72-73	2.188544018756774E-4	7.707481109534725E-4	1.04669496549237E-4	0.09746157440050546	1.7127735798966055E-4
74-75	2.188544018756774E-4	7.897789285078792E-4	1.04669496549237E-4	0.10111073366656295	1.7127735798966055E-4
76-77	2.188544018756774E-4	8.183251548394893E-4	1.04669496549237E-4	0.10444112673858413	1.7127735798966055E-4
78-79	2.188544018756774E-4	8.278405636166926E-4	1.04669496549237E-4	0.10740041886829438	1.7127735798966055E-4
80-81	2.188544018756774E-4	8.278405636166926E-4	1.04669496549237E-4	0.10883248788926347	1.807927667668639E-4
82-83	2.2836981065288076E-4	8.278405636166926E-4	1.04669496549237E-4	0.10923213505790602	2.188544018756774E-4
84-85	2.2836981065288076E-4	8.278405636166926E-4	1.04669496549237E-4	0.10958420518266254	2.188544018756774E-4
86-87	2.2836981065288076E-4	8.278405636166926E-4	1.04669496549237E-4	0.1097269363143206	2.2361210626427907E-4
88-89	2.2836981065288076E-4	8.37355972393896E-4	1.04669496549237E-4	0.10976975565381801	2.2836981065288076E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14085	0.0	47.88905	1
GTATCAA	25820	0.0	36.58327	1
TCAACGC	31700	0.0	29.483725	4
ATCAACG	31765	0.0	29.302526	3
CAACGCA	32410	0.0	28.824667	5
TATCAAC	33165	0.0	28.352592	2
AACGCAG	33315	0.0	28.106068	6
CTTATAC	7765	0.0	24.450048	1
ACGCAGA	38455	0.0	24.304962	7
CGCAGAG	38845	0.0	24.049963	8
GCAGAGT	45080	0.0	20.581776	9
GTACATG	29160	0.0	20.498014	1
TACATGG	29065	0.0	19.728321	2
GAGTACT	28080	0.0	19.620586	12-13
TATACAC	13285	0.0	19.137444	3
AGAGTAC	40885	0.0	19.08387	10-11
ACATGGG	29795	0.0	18.855654	3
CAGAGTA	44040	0.0	18.070274	10-11
GTGGTAT	7620	0.0	17.860636	1
AGTACTT	30650	0.0	17.724874	12-13
>>END_MODULE