FastQCFastQC Report
Fri 27 May 2016
SRR2139070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10780114
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148200.048.8022231
GTATCAA273250.036.450321
CTTATAC95800.035.9545631
ATCAACG335600.029.1416533
TCAACGC337350.029.0784844
CAACGCA345700.028.3883915
TATCAAC352100.028.1188982
AACGCAG355850.027.6620886
TATACAC152250.025.9061263
ACGCAGA403650.024.3443367
CGCAGAG410400.023.9129318
TTATACA157000.023.6953642
ACATCTC164950.021.2632688
GCAGAGT465550.020.8888539
ACACATC169800.020.4810876
GTACATG297800.019.827961
TACATGG294300.019.602532
TACACAT195250.019.5057265
GAGTACT297950.019.18825112-13
AGAGTAC428800.019.14376310-11
Produced by FastQC (version 0.11.5)