##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139070_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10780114
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57143922596737	34.0	31.0	34.0	31.0	34.0
2	32.70964481451681	34.0	31.0	34.0	31.0	34.0
3	32.69689541316539	34.0	31.0	34.0	31.0	34.0
4	36.21865371739111	37.0	35.0	37.0	35.0	37.0
5	36.1771633398311	37.0	35.0	37.0	35.0	37.0
6	36.21800966112232	37.0	37.0	37.0	35.0	37.0
7	36.17818262404275	37.0	37.0	37.0	35.0	37.0
8	36.15528963793889	37.0	37.0	37.0	35.0	37.0
9	37.885679594854004	39.0	38.0	39.0	35.0	39.0
10-11	37.817995013781854	39.0	38.0	39.0	35.0	39.0
12-13	37.787689675637935	39.0	38.0	39.0	35.0	39.0
14-15	39.134588465391	40.0	39.0	41.0	36.0	41.0
16-17	39.107894499074874	40.0	38.0	41.0	36.0	41.0
18-19	39.072863283263985	40.0	38.5	41.0	36.0	41.0
20-21	39.059773143018816	40.0	39.0	41.0	36.0	41.0
22-23	38.94485037468152	40.0	38.0	41.0	35.0	41.0
24-25	38.808246710295315	40.0	38.0	41.0	35.0	41.0
26-27	38.757077557735855	40.0	38.0	41.0	34.5	41.0
28-29	38.70095164333322	40.0	38.0	41.0	35.0	41.0
30-31	38.62759907223744	40.0	38.0	41.0	35.0	41.0
32-33	38.51335093360783	40.0	38.0	41.0	34.0	41.0
34-35	38.31819894280933	40.0	38.0	41.0	34.0	41.0
36-37	38.449178849634606	40.0	38.0	41.0	34.0	41.0
38-39	38.42656440482568	40.0	38.0	41.0	34.0	41.0
40-41	38.407597269988365	40.0	38.0	41.0	34.0	41.0
42-43	38.446632527320986	40.0	38.0	41.0	34.0	41.0
44-45	38.38988215478859	40.0	38.0	41.0	34.0	41.0
46-47	38.33891531581469	40.0	38.0	41.0	34.0	41.0
48-49	38.18937935432116	40.0	38.0	41.0	33.0	41.0
50-51	38.19172764159907	40.0	38.0	41.0	33.0	41.0
52-53	38.1106510394703	40.0	37.0	41.0	33.0	41.0
54-55	37.965603958499926	40.0	37.0	41.0	33.0	41.0
56-57	37.80392951382845	40.0	37.0	41.0	33.0	41.0
58-59	37.64988652565013	39.5	36.0	41.0	33.0	41.0
60-61	37.468844306387766	39.0	36.0	41.0	32.0	41.0
62-63	37.26748406676569	39.0	35.5	41.0	32.0	41.0
64-65	36.989531332454035	38.5	35.0	40.0	31.5	41.0
66-67	36.64701785844164	38.0	35.0	40.0	31.0	41.0
68-69	36.32818168133318	37.5	35.0	40.0	31.0	41.0
70-71	36.03616035035289	37.0	35.0	39.5	31.0	41.0
72-73	35.7158740815927	36.5	34.0	39.0	30.5	41.0
74-75	35.35154470299301	36.0	34.0	39.0	30.0	40.0
76-77	34.384498620921896	35.0	33.0	37.0	29.0	39.0
78-79	34.66539791642874	35.0	34.0	37.0	30.0	39.0
80-81	34.464444346339576	35.0	34.0	37.0	30.0	39.0
82-83	34.181389462510054	35.0	34.0	36.5	29.5	39.0
84-85	33.924195667546755	35.0	33.5	36.0	29.0	37.0
86-87	33.67459554450056	35.0	33.0	36.0	29.0	37.0
88-89	33.428431424799115	35.0	33.0	35.0	29.0	36.5
90-91	33.23540394161614	35.0	33.0	35.0	29.0	36.0
92-93	33.13727601488542	35.0	33.0	35.0	29.0	36.0
94-95	33.053869810946715	34.5	33.0	35.0	29.0	36.0
96-97	33.028418248311056	34.0	33.0	35.0	29.0	35.0
98-99	33.14254131069195	34.0	33.0	35.0	29.0	35.0
100-101	31.282806393278165	32.5	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	1.0
19	6.0
20	57.0
21	330.0
22	1519.0
23	5061.0
24	12692.0
25	26128.0
26	46504.0
27	72326.0
28	105087.0
29	141450.0
30	182707.0
31	229617.0
32	292241.0
33	382060.0
34	526349.0
35	758171.0
36	1251477.0
37	2325961.0
38	2917982.0
39	1421261.0
40	81124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.048624916756424	21.85813300067133	15.925503921063521	24.167738161508726
2	13.291532077297841	21.89581001523037	41.9954921220686	22.81716578540319
3	16.585283769341522	27.112707837609463	31.826945010120955	24.47506338292806
4	11.635770084942603	19.431947913670214	39.4308291859549	29.501452815432284
5	12.503544722313157	38.37955842940764	36.18419142437402	12.932705423905189
6	28.628953820605375	39.30297192672691	18.952420805314528	13.115653447353187
7	25.311047353779564	33.49873670131968	23.495611501588435	17.69460444331232
8	23.85037151280325	37.36459905383181	21.422011067973035	17.363018365391902
9	24.851502020433365	17.212583949722976	21.684652099657953	36.2512619301857
10-11	23.390347311088345	27.520218958906245	29.396796227866055	19.692637502139355
12-13	24.36067211738274	24.92392545620028	29.50036463183873	21.215037794578254
14-15	21.485631005170088	26.46006720169023	27.21135063300979	24.842951160129893
16-17	20.886857084759654	29.747547532978125	27.81930332352945	21.546292058732767
18-19	20.926309313612084	28.627841969018135	29.8344062038676	20.611442513502176
20-21	21.738955187341794	27.67155739319202	29.775452933262425	20.814034486203763
22-23	21.378069441423484	27.85175363485981	29.58393213304809	21.186244790668617
24-25	21.241078469562684	28.016734184504315	29.764951618467645	20.977235727465356
26-27	21.29704068171863	28.114519482466903	29.670419560762134	20.918020275052328
28-29	21.11383280641979	28.031163478604377	29.80322233023455	21.05178138474128
30-31	21.482036633458204	28.000887814687438	29.600684912272325	20.916390639582037
32-33	21.33793226270644	27.871087254307813	29.628981107945286	21.161999375040462
34-35	21.186118143438186	28.20122627095842	29.75217217402712	20.86048341157628
36-37	21.38963345101883	28.03357522545432	29.518500735516746	21.058290588010106
38-39	21.22516754344625	28.02163223900207	29.576755676824636	21.176444540727044
40-41	21.39915398634995	27.953852626346887	29.502950711651167	21.144042675652
42-43	21.4748072150011	28.005048474260736	29.284982707463907	21.235161603274257
44-45	21.400061917478308	28.296229665770145	28.99064374194718	21.313064674804366
46-47	21.629672966123405	28.08365222660958	28.590593305820693	21.696081501446322
48-49	21.587076472126643	28.22115831505779	28.546647146494248	21.645118066321324
50-51	21.386278836147007	28.301691480992396	28.617767436530862	21.694262246329732
52-53	21.57492261949996	28.289087114466412	28.606813806475234	21.5291764595584
54-55	21.580245529147014	28.281136132370115	28.433844969621347	21.704773368861527
56-57	21.80741828738216	28.50275056065258	28.11598102058477	21.5738501313805
58-59	21.440144289714237	28.7989751423154	28.469371845625524	21.29150872234484
60-61	21.7858053748142	28.88986851559493	28.011732586915116	21.312593522675755
62-63	21.56722312780262	29.055520913327236	27.969652710498615	21.407603248371522
64-65	21.519410596854478	28.91836762205287	28.084638021028134	21.477583760064515
66-67	21.65607511697502	28.76931947726038	27.998554776057972	21.57605062970663
68-69	21.64980210260667	28.573230103361908	28.125156855688	21.651810938343417
70-71	21.63765427882367	28.331558918879157	28.22684547860369	21.80394132369348
72-73	21.698019877263636	28.341422210056415	28.215472966340815	21.74508494633913
74-75	21.587320423659705	28.45460483986494	28.152518342861498	21.805556393613863
76-77	21.65157794092369	28.437800199988388	28.067949296821936	21.84267256226598
78-79	21.631613107733568	28.436414639137208	28.078485350083405	21.853486903045816
80-81	21.643387259340326	28.36057863867388	28.165305099451608	21.830729002534188
82-83	21.6679472291318	28.316890677388308	28.27183709842087	21.743324995059016
84-85	21.833694884210484	28.291914076686673	28.140734603896817	21.73365643520603
86-87	21.626495729930483	28.290392917891932	28.21596967552774	21.867141676649844
88-89	21.717094979192208	28.25489903881523	28.216898526584146	21.811107455408422
90-91	21.70686417408741	28.281100681064338	28.286812275229607	21.725222869618648
92-93	21.76200899128474	28.167714358251892	28.315526811938625	21.754749838524738
94-95	21.53145523006036	28.259912716548925	28.374613521404505	21.834018531986207
96-97	21.441058132361615	28.310823751357095	28.478398894719376	21.769719221561914
98-99	21.660058515404877	28.806324113945468	28.49273830340663	21.040879067243022
100-101	20.194694632862088	32.415883624836454	26.394293718491397	20.995128023810064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2098.0
1	1949.5
2	2792.0
3	4045.0
4	5618.0
5	8653.0
6	11140.5
7	13534.0
8	15621.0
9	16015.5
10	14850.5
11	13612.0
12	15220.5
13	21080.5
14	35545.5
15	55555.0
16	70967.5
17	78718.5
18	79251.0
19	74491.0
20	67773.0
21	61573.5
22	58742.0
23	62582.0
24	73208.5
25	90247.5
26	112960.0
27	137906.5
28	163706.0
29	191446.5
30	217826.0
31	243400.5
32	270315.0
33	296496.0
34	321612.5
35	344526.5
36	368118.0
37	387837.5
38	397947.5
39	405123.5
40	416624.0
41	427138.5
42	430724.0
43	433803.5
44	437889.5
45	445109.0
46	455624.5
47	459060.0
48	445055.5
49	425778.0
50	409574.0
51	387406.0
52	359722.0
53	332662.0
54	309874.0
55	284184.0
56	256496.5
57	229615.0
58	202105.5
59	173579.0
60	143999.0
61	116112.0
62	89921.0
63	68121.0
64	51012.0
65	38045.0
66	29024.0
67	21999.0
68	16436.5
69	11685.0
70	8085.0
71	5804.0
72	4172.0
73	2921.5
74	1936.5
75	1248.0
76	836.5
77	504.0
78	324.0
79	230.0
80	163.0
81	109.0
82	78.5
83	52.0
84	35.5
85	29.0
86	20.5
87	14.0
88	6.5
89	2.0
90	1.0
91	1.0
92	0.5
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.78290192478484E-5
2	0.01530596058631662
3	2.78290192478484E-5
4	1.0203973724211079E-4
5	2.78290192478484E-5
6	2.78290192478484E-5
7	2.78290192478484E-5
8	9.276339749282799E-6
9	9.276339749282799E-6
10-11	8.34870577435452E-5
12-13	5.797712343301749E-4
14-15	2.597375129799184E-4
16-17	0.0018877351389790498
18-19	0.0
20-21	0.0
22-23	2.829606216227374E-4
24-25	0.009195902747436015
26-27	0.01987045816607136
28-29	0.34807859648705625
30-31	0.32464726036648905
32-33	0.3180557687912553
34-35	0.18673891101026358
36-37	0.04581001009256184
38-39	0.03792638873486684
40-41	0.015910159178055532
42-43	0.014852157178968352
44-45	0.01815009116789346
46-47	0.012681305245160443
48-49	0.007793085362247272
50-51	0.004423398137853639
52-53	0.0018610296146342965
54-55	2.1177301663855212E-4
56-57	4.068672064268744E-4
58-59	0.001483151780672541
60-61	0.001528304827080857
62-63	4.760377505259825E-4
64-65	1.9031385980244046E-4
66-67	0.00294258025446257
68-69	0.00507868523992754
70-71	0.006917657978730391
72-73	0.010743461942654573
74-75	0.007988850916086563
76-77	0.006002227769467701
78-79	0.0038594193926004654
80-81	0.004407642841133071
82-83	0.001438419338530588
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0024044986005385457
92-93	0.014855781307804367
94-95	0.0012571901978676258
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	190.0
20-21	729.0
22-23	1539.0
24-25	4199.0
26-27	5811.0
28-29	9470.0
30-31	21140.0
32-33	28113.0
34-35	41137.0
36-37	55548.0
38-39	67079.0
40-41	71808.0
42-43	87153.0
44-45	104617.0
46-47	102408.0
48-49	88048.0
50-51	77744.0
52-53	76681.0
54-55	83397.0
56-57	87964.0
58-59	90984.0
60-61	93091.0
62-63	97696.0
64-65	106837.0
66-67	116310.0
68-69	100539.0
70-71	94303.0
72-73	92314.0
74-75	94686.0
76-77	95835.0
78-79	101209.0
80-81	104664.0
82-83	104026.0
84-85	108448.0
86-87	116602.0
88-89	125628.0
90-91	136891.0
92-93	147787.0
94-95	177472.0
96-97	272361.0
98-99	1014960.0
100-101	6372696.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.41881326341833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.74107220926784	74.02674931420437
2	7.619458993783752	12.712124539414402
3	2.0013355149414322	5.008471007950395
4	0.7560482077831024	2.5227457705280285
5	0.3414316985777368	1.4240913552933978
6	0.1778512960854104	0.8901686434083464
7	0.1108730455601559	0.6474228518470382
8	0.06775543974388652	0.452166270046085
9	0.04211137840896279	0.3161593090586172
>10	0.1394149261574024	1.7485800757868974
>50	0.002244826314753175	0.11932118426748527
>100	3.0753365789461094E-4	0.05243161954079392
>500	5.6959229004745554E-5	0.0312010498403191
>1k	3.797048860981862E-5	0.048367008813867234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.78290192478484E-5	0.0	0.0	0.0
3	0.0	2.78290192478484E-5	0.0	0.0	0.0
4	0.0	1.020397372421108E-4	0.0	1.85526794985656E-5	0.0
5	0.0	1.020397372421108E-4	0.0	1.85526794985656E-5	0.0
6	8.34870577435452E-5	1.020397372421108E-4	0.0	1.85526794985656E-5	9.2763397492828E-6
7	8.34870577435452E-5	1.020397372421108E-4	0.0	1.85526794985656E-5	9.2763397492828E-6
8	8.34870577435452E-5	1.113160769913936E-4	0.0	1.85526794985656E-5	9.2763397492828E-6
9	8.34870577435452E-5	2.4118483348135278E-4	0.0	4.6381698746413996E-5	2.78290192478484E-5
10-11	8.81252276181866E-5	2.4118483348135278E-4	0.0	4.6381698746413996E-5	2.78290192478484E-5
12-13	9.276339749282799E-5	2.504611732306356E-4	0.0	4.6381698746413996E-5	2.78290192478484E-5
14-15	9.276339749282799E-5	3.339482309741808E-4	0.0	7.421071799426239E-5	4.17435288717726E-5
16-17	1.5769777573780759E-4	3.432245707234636E-4	0.0	8.34870577435452E-5	4.6381698746413996E-5
18-19	1.5769777573780759E-4	3.756917598459534E-4	0.0	9.2763397492828E-5	4.6381698746413996E-5
20-21	1.5769777573780759E-4	4.081589489684432E-4	0.0	1.020397372421108E-4	4.6381698746413996E-5
22-23	1.5769777573780759E-4	4.777314970880642E-4	0.0	1.020397372421108E-4	6.02962083703382E-5
24-25	1.5769777573780759E-4	5.241131958344783E-4	0.0	1.15954246866035E-4	8.34870577435452E-5
26-27	1.5769777573780759E-4	5.473040452076852E-4	0.0	1.6233594561244902E-4	8.34870577435452E-5
28-29	1.669741154870904E-4	5.473040452076852E-4	0.0	3.803299297205948E-4	8.34870577435452E-5
30-31	1.669741154870904E-4	6.447056125751546E-4	0.0	0.001057502731418239	8.34870577435452E-5
32-33	1.669741154870904E-4	6.539819523244374E-4	0.0	0.0023840193155656797	8.34870577435452E-5
34-35	1.948031347349388E-4	7.699361991904724E-4	0.0	0.004322774323165785	8.34870577435452E-5
36-37	2.040794744842216E-4	8.858904460565073E-4	0.0	0.007096399908201342	8.34870577435452E-5
38-39	2.040794744842216E-4	8.905286159311488E-4	0.0	0.010913613715031214	9.276339749282799E-5
40-41	2.040794744842216E-4	0.0010111210326718251	0.0	0.015333789605564467	1.020397372421108E-4
42-43	2.1335581423350438E-4	0.001034311882045032	0.0	0.0197400509864738	1.020397372421108E-4
44-45	2.272703238574286E-4	0.0011363516192871431	0.0	0.025375427384163096	1.020397372421108E-4
46-47	2.3190849373207E-4	0.0012152005071560468	0.0	0.031173139727464848	1.0667790711675219E-4
48-49	2.3654666360671138E-4	0.0012152005071560468	0.0	0.037045062788760866	1.113160769913936E-4
50-51	2.55099343105277E-4	0.0013357929238967232	0.0	0.04256912310945877	1.113160769913936E-4
52-53	2.597375129799184E-4	0.0013357929238967232	0.0	0.048065354410908825	1.2059241674067639E-4
54-55	2.78290192478484E-4	0.0013404310937713646	0.0	0.05376102701696847	1.298687564899592E-4
56-57	3.061192117263324E-4	0.0013543456033952887	0.0	0.060027194517609	1.298687564899592E-4
58-59	3.1539555147561517E-4	0.0013543456033952887	0.0	0.06554197849855763	1.298687564899592E-4
60-61	3.525009104727464E-4	0.0013868127925177787	0.0	0.07101501895063447	1.298687564899592E-4
62-63	3.803299297205948E-4	0.0014239181515149097	0.0	0.07613092032236393	1.298687564899592E-4
64-65	3.803299297205948E-4	0.0016372739657484143	0.0	0.0814601775083269	1.298687564899592E-4
66-67	3.849680995952362E-4	0.0016558266452469798	0.0	0.087318186059999	1.345069263646006E-4
68-69	3.9424443934451896E-4	0.001669741154870904	0.0	0.09293964794806438	1.39145096239242E-4
70-71	3.988826092191604E-4	0.0016975701741187522	0.0	0.09838949755076801	1.484214359885248E-4
72-73	4.17435288717726E-4	0.0017207610234919594	0.0	0.10230875109484	1.5769777573780759E-4
74-75	4.17435288717726E-4	0.0017764190619876561	0.0	0.10580129301044497	1.5769777573780759E-4
76-77	4.17435288717726E-4	0.00185526794985656	0.0	0.10870942552184512	1.5769777573780759E-4
78-79	4.17435288717726E-4	0.001901649648602974	0.0	0.1115294328056271	1.5769777573780759E-4
80-81	4.359879682162916E-4	0.0019109259883522568	0.0	0.11274463331278314	1.669741154870904E-4
82-83	4.4062613809093295E-4	0.0019248404979761808	0.0	0.113199173960498	1.762504552363732E-4
84-85	4.452643079655744E-4	0.0019619458569733123	0.0	0.11364443826846359	1.762504552363732E-4
86-87	4.452643079655744E-4	0.002003689385845085	0.0	0.11376966885507889	1.762504552363732E-4
88-89	4.452643079655744E-4	0.00208717644358863	0.0	0.11381141238395068	1.762504552363732E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14820	0.0	48.802223	1
GTATCAA	27325	0.0	36.45032	1
CTTATAC	9580	0.0	35.954563	1
ATCAACG	33560	0.0	29.141653	3
TCAACGC	33735	0.0	29.078484	4
CAACGCA	34570	0.0	28.388391	5
TATCAAC	35210	0.0	28.118898	2
AACGCAG	35585	0.0	27.662088	6
TATACAC	15225	0.0	25.906126	3
ACGCAGA	40365	0.0	24.344336	7
CGCAGAG	41040	0.0	23.912931	8
TTATACA	15700	0.0	23.695364	2
ACATCTC	16495	0.0	21.263268	8
GCAGAGT	46555	0.0	20.888853	9
ACACATC	16980	0.0	20.481087	6
GTACATG	29780	0.0	19.82796	1
TACATGG	29430	0.0	19.60253	2
TACACAT	19525	0.0	19.505726	5
GAGTACT	29795	0.0	19.188251	12-13
AGAGTAC	42880	0.0	19.143763	10-11
>>END_MODULE