FastQCFastQC Report
Fri 27 May 2016
SRR2139069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9167883
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118300.050.9612581
GTATCAA228650.038.6240651
ATCAACG285100.030.4822793
TCAACGC287450.030.3656124
CAACGCA294650.029.609245
TATCAAC298450.029.3071542
AACGCAG302100.028.9354176
ACGCAGA348650.025.0356837
CGCAGAG354750.024.605198
GCAGAGT407400.021.3318429
CTTATAC64400.020.5108491
GTACATG264950.019.7660871
GAGTACT249650.019.72093612-13
AGAGTAC369500.019.56823310-11
ACCGAAC10900.019.4179748
TACATGG263300.019.359342
ACATGGG267350.018.4459673
GGACCGA13400.018.3224536
AGTACTT271650.018.2640612-13
GTGGTAT62150.018.2561241
Produced by FastQC (version 0.11.5)