FastQCFastQC Report
Fri 27 May 2016
SRR2139068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12934512
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189600.046.700191
GTATCAA335650.034.733941
CTTATAC114900.033.1624261
ATCAACG391250.029.1451683
TCAACGC412150.027.6980824
CAACGCA419650.027.1833135
TATCAAC423850.027.1265982
AACGCAG423400.026.95266
ACGCAGA431000.026.4781537
TATACAC170350.025.0063513
CGCAGAG495900.022.9958978
TTATACA181400.021.5375632
ACATCTC180550.020.8903648
ACACATC184500.020.7162676
GCAGAGT567650.020.0299289
GAGTACT335050.019.2601112-13
GTACATG381750.018.9479331
TACATGG375950.018.82452
ACATGGG364350.018.7620013
AGAGTAC512850.018.6734610-11
Produced by FastQC (version 0.11.5)