##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12934512
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57906490789912	34.0	31.0	34.0	31.0	34.0
2	32.711763922751786	34.0	31.0	34.0	31.0	34.0
3	33.418217092380445	34.0	31.0	34.0	31.0	37.0
4	36.16794286479459	37.0	35.0	37.0	35.0	37.0
5	36.11031935337027	37.0	35.0	37.0	35.0	37.0
6	36.14247186132728	37.0	36.0	37.0	35.0	37.0
7	36.146321020847175	37.0	36.0	37.0	35.0	37.0
8	36.41692852424583	37.0	37.0	37.0	35.0	39.0
9	37.758711267962795	39.0	38.0	39.0	35.0	39.0
10-11	37.70546465146887	39.0	37.0	39.0	35.0	39.0
12-13	37.82192200215981	39.0	37.5	39.0	35.0	40.0
14-15	38.979795256287986	40.0	38.0	41.0	35.0	41.0
16-17	38.94298787615644	40.0	38.0	41.0	35.0	41.0
18-19	38.94484967813243	40.0	38.0	41.0	35.0	41.0
20-21	38.90865701743013	40.0	38.0	41.0	35.0	41.0
22-23	38.80767991145236	40.0	38.0	41.0	35.0	41.0
24-25	38.67096982241844	40.0	38.0	41.0	34.0	41.0
26-27	38.61814344917696	40.0	38.0	41.0	34.0	41.0
28-29	38.55768804250826	40.0	38.0	41.0	34.0	41.0
30-31	38.47153754943716	40.0	38.0	41.0	34.0	41.0
32-33	38.3617198615934	40.0	38.0	41.0	34.0	41.0
34-35	38.27362624982449	40.0	38.0	41.0	34.0	41.0
36-37	38.423197109818204	40.0	38.0	41.0	34.0	41.0
38-39	38.46954597286274	40.0	38.0	41.0	34.0	41.0
40-41	38.405864148334445	40.0	38.0	41.0	34.0	41.0
42-43	38.37869379189719	40.0	38.0	41.0	34.0	41.0
44-45	38.34761692506058	40.0	38.0	41.0	34.0	41.0
46-47	38.25912950112632	40.0	38.0	41.0	33.0	41.0
48-49	38.18905435059025	40.0	38.0	41.0	33.0	41.0
50-51	38.11518452370041	40.0	37.0	41.0	33.0	41.0
52-53	38.047479232873044	40.0	37.0	41.0	33.0	41.0
54-55	37.919148139208964	40.0	37.0	41.0	33.0	41.0
56-57	37.76494837808579	40.0	36.5	41.0	33.0	41.0
58-59	37.649017885183795	39.5	36.0	41.0	33.0	41.0
60-61	37.49526777038655	39.0	36.0	41.0	32.5	41.0
62-63	37.27274435834978	39.0	35.5	41.0	32.0	41.0
64-65	36.96018960840978	38.5	35.0	40.0	31.5	41.0
66-67	36.67785714764777	38.0	35.0	40.0	31.0	41.0
68-69	36.40212796897613	37.5	35.0	40.0	31.0	41.0
70-71	36.09890057425722	37.0	35.0	39.5	31.0	41.0
72-73	35.78424830688212	36.5	34.5	39.0	31.0	41.0
74-75	35.35892527811258	36.0	34.0	39.0	30.0	40.0
76-77	34.57649499330605	35.0	33.5	37.0	29.5	39.0
78-79	34.82164658168533	35.0	34.0	37.0	30.0	39.0
80-81	34.64443141357629	35.0	34.0	37.0	30.0	39.0
82-83	34.39626227975178	35.0	34.0	36.5	30.0	39.0
84-85	34.133213938430984	35.0	34.0	36.0	30.0	37.0
86-87	33.90244927662198	35.0	34.0	36.0	30.0	37.0
88-89	33.69710751863999	35.0	33.5	35.0	30.0	37.0
90-91	33.5302564254408	35.0	33.0	35.0	29.5	36.0
92-93	33.40529223212073	35.0	33.0	35.0	29.0	36.0
94-95	33.355502834261756	35.0	33.0	35.0	29.0	36.0
96-97	33.373209926382245	35.0	33.0	35.0	30.0	35.5
98-99	33.37692850734021	35.0	33.0	35.0	30.0	35.0
100-101	31.67057003963961	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	4.0
19	18.0
20	71.0
21	461.0
22	2063.0
23	6519.0
24	16566.0
25	34485.0
26	59956.0
27	93749.0
28	132483.0
29	176448.0
30	226031.0
31	283002.0
32	353777.0
33	454271.0
34	616468.0
35	863784.0
36	1388639.0
37	2619750.0
38	3593474.0
39	1878835.0
40	133657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.048785941481704	0.00674977367382604	27.400176607480248	30.544287677364217
2	13.773486307373773	25.427103908564476	38.247497567315605	22.551912216746146
3	15.198254097255468	25.02756965241518	34.52611895988036	25.248057290448994
4	12.258243681709832	25.032332104991667	37.71983821268247	24.989586000616026
5	16.69732882075489	36.85937281592069	32.854521299296024	13.588777064028394
6	27.008595299150056	38.59362456040088	20.51355319783228	13.884226942616776
7	25.105168250646027	33.560632206302024	23.06925843046881	18.26494111258314
8	23.564520307651414	32.84546015623786	22.46277767482322	21.12724186128751
9	23.088431349688754	20.909222059316058	24.438687578994113	31.56365901200108
10-11	23.931804295365918	26.697971177903106	29.209585315990605	20.16063921074037
12-13	24.263527045780325	24.783211121467296	29.08576694966999	21.86749488308239
14-15	21.653304931794676	27.305791742176865	27.163834302040268	23.87706902398819
16-17	20.756435215972402	29.710426249959266	28.141847490722782	21.391291043345547
18-19	20.879652891400376	28.683372722787897	29.934243358716845	20.502731027094878
20-21	21.673389115393064	27.942244901761843	29.7871498180729	20.597216164772195
22-23	21.38539286576863	27.837266799929466	29.59469105631304	21.182649277988872
24-25	21.275681862007655	28.046971439807077	29.78707308860859	20.890273609576678
26-27	21.18671349385908	28.264856487331897	29.65827566776519	20.890154351043833
28-29	21.059675425448646	28.053807595503272	29.707588811231073	21.178928167817006
30-31	21.268402461944817	28.050043885523536	29.6962779553896	20.985275697142054
32-33	20.969589980767893	28.1742377402878	29.782940257385075	21.073232021559228
34-35	21.029497131412324	28.00056097727769	29.675120306440668	21.294821584869318
36-37	21.125849197908426	28.164906788346073	29.781675645560412	20.927568368185085
38-39	21.08520001027767	28.297020468173184	29.66421489540498	20.953564626144157
40-41	21.369904000985773	28.115951159324737	29.377885874631392	21.136258965058097
42-43	21.40583194615381	28.106341755864477	29.326012431191156	21.161813866790553
44-45	21.32990009189957	28.55250186655872	28.95680591049492	21.160792131046787
46-47	21.489052263077188	28.18581332585929	28.644962163259617	21.680172247803906
48-49	21.425176062685217	28.344393754139546	28.786404056780924	21.444026126394313
50-51	21.30502153335336	28.569533363840772	28.531725427574955	21.593719675230908
52-53	21.487659848477225	28.411639383847632	28.584001569558616	21.51669919811653
54-55	21.484710089335803	28.488450520164893	28.52028397402952	21.506555416469787
56-57	21.625550507311264	28.822677340336018	28.101717065329574	21.450055087023152
58-59	21.479600429475994	28.8017175589392	28.478566849223437	21.240115162361377
60-61	21.653479965280923	28.904137920395556	28.165272433116577	21.277109681206944
62-63	21.475587357520975	29.04983511067856	28.099127261687933	21.375450270112527
64-65	21.463640446517473	28.85435124860291	28.157534548397134	21.524473756482486
66-67	21.50034827559105	28.817856032126482	28.12024189445707	21.5615537978254
68-69	21.607887042014564	28.74789920793836	28.205443261539916	21.43877048850716
70-71	21.582463333067682	28.342131109556572	28.28936566723634	21.786039890139406
72-73	21.60355217561011	28.467129221783754	28.2983863322969	21.630932270309238
74-75	21.503420464707997	28.60300499292147	28.239725287961598	21.653849254408936
76-77	21.633353835004456	28.432256280424717	28.10833464448672	21.826055240084106
78-79	21.47515496334791	28.49964543000333	28.251509153927184	21.773690452721574
80-81	21.53589992411166	28.55242804835067	28.178579654548063	21.733092372989613
82-83	21.647078785528283	28.334316107738456	28.336789533254713	21.681815573478545
84-85	21.73509984277663	28.36080295501169	28.303503281779157	21.60059392043252
86-87	21.57483106747642	28.40889343801515	28.23344309939853	21.782832395109896
88-89	21.627958054798032	28.300666805121782	28.283123776141885	21.7882513639383
90-91	21.702620526108625	28.3067710745399	28.31794993159492	21.67265846775656
92-93	21.65113784162062	28.312031044697942	28.33120909261965	21.70562202106179
94-95	21.577381291201224	28.248649408192122	28.366764864912632	21.80720443569403
96-97	21.414546346369555	28.392751585075317	28.552556413840673	21.640145654714456
98-99	21.85454214552209	29.3095958570728	28.37782075859981	20.4580412388053
100-101	21.935583569983052	31.552904474660153	25.46258859487398	21.048923360482817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2334.0
1	2104.0
2	3257.0
3	4927.0
4	6949.0
5	11009.0
6	14020.0
7	16356.0
8	18187.5
9	18331.0
10	17057.5
11	15639.5
12	17453.5
13	23668.0
14	39574.5
15	63956.0
16	85151.5
17	96545.5
18	97399.5
19	91160.0
20	82552.0
21	74924.5
22	71884.0
23	76857.5
24	89055.5
25	108781.0
26	137220.5
27	169474.5
28	201493.5
29	233025.5
30	263104.0
31	291948.0
32	323944.5
33	355607.0
34	389515.5
35	420955.5
36	450571.5
37	476957.5
38	490347.5
39	499200.5
40	510204.5
41	520445.0
42	524085.5
43	528032.5
44	533980.5
45	535513.5
46	536972.0
47	536286.0
48	535275.5
49	523863.0
50	500434.5
51	475692.0
52	446832.5
53	414703.5
54	381875.0
55	348107.0
56	311647.0
57	276142.5
58	240063.0
59	205481.5
60	170732.5
61	136514.5
62	106116.0
63	79836.5
64	58224.0
65	42741.0
66	31807.5
67	23735.0
68	17925.0
69	12534.0
70	8477.0
71	6100.5
72	4417.0
73	3093.5
74	2075.0
75	1336.5
76	889.0
77	591.0
78	383.5
79	246.5
80	162.0
81	106.5
82	81.0
83	59.0
84	43.0
85	32.0
86	21.5
87	16.5
88	11.0
89	7.0
90	5.0
91	2.5
92	2.5
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005759784366043342
2	0.017750959603269143
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	2.319376254782554E-5
9	3.092501673043405E-5
10-11	4.716065051391193E-4
12-13	0.0016351602596217005
14-15	1.159688127391277E-5
16-17	3.865627091304256E-6
18-19	7.731254182608512E-6
20-21	4.63892430149204E-5
22-23	6.959113622337333E-4
24-25	0.01173262666364315
26-27	0.06430059609875156
28-29	0.32378224427493685
30-31	0.31188118044216184
32-33	0.30995849488985405
34-35	0.13731152018590825
36-37	0.028383246350248557
38-39	0.024813158648906573
40-41	0.010333521650691426
42-43	0.00735457849891374
44-45	0.0028041978721100973
46-47	0.001978048635852013
48-49	0.0020683139372593036
50-51	0.001567337647932561
52-53	1.7187978761191942E-4
54-55	3.805804672487884E-5
56-57	2.1348203979868816E-4
58-59	4.744138885959743E-4
60-61	0.00234005781119359
62-63	0.001990372147190134
64-65	0.002363483143891925
66-67	0.007782391016238401
68-69	0.011164376039708162
70-71	0.015235596551074109
72-73	0.013683511885680249
74-75	0.010163010732535295
76-77	0.0047771379337076285
78-79	0.0022916980367963724
80-81	0.001909995165904818
82-83	1.830331531907848E-4
84-85	2.0041854406650126E-5
86-87	2.0312625525678054E-5
88-89	8.140311352483365E-4
90-91	0.004013969661088034
92-93	0.013592583026653119
94-95	2.9816094868959076E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	265.0
20-21	1105.0
22-23	2192.0
24-25	4878.0
26-27	7953.0
28-29	14376.0
30-31	27335.0
32-33	36717.0
34-35	54496.0
36-37	72977.0
38-39	85697.0
40-41	91368.0
42-43	111016.0
44-45	131521.0
46-47	127974.0
48-49	112353.0
50-51	100721.0
52-53	100728.0
54-55	110260.0
56-57	117868.0
58-59	117960.0
60-61	119241.0
62-63	121052.0
64-65	126893.0
66-67	137068.0
68-69	127074.0
70-71	119875.0
72-73	117007.0
74-75	119276.0
76-77	122135.0
78-79	127134.0
80-81	128836.0
82-83	127561.0
84-85	131789.0
86-87	138445.0
88-89	148373.0
90-91	157999.0
92-93	165945.0
94-95	193613.0
96-97	343362.0
98-99	1298324.0
100-101	7433750.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.12016282672214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.31133144771024	70.8270942365616
2	8.200084228151196	13.30384335560922
3	2.3759287170714956	5.782071731805742
4	0.9575046688799258	3.1069173858754495
5	0.469859211649243	1.9057527877310945
6	0.2390586513918152	1.1635486035624392
7	0.13761535510173475	0.7814366009316938
8	0.08752377331568936	0.5679954194062271
9	0.0572076955756088	0.41766278220314434
>10	0.16136566629796942	1.9238533179554933
>50	0.0020056689978226803	0.10954970802919432
>100	4.8072321249992376E-4	0.0770865974846435
>500	1.7096329079245184E-5	0.013854701416489461
>1k	1.7096315696336545E-5	0.01933277142755269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.319376254782554E-5	0.0	0.0	0.0
3	0.0	2.319376254782554E-5	0.0	0.0	0.0
4	0.0	2.319376254782554E-5	0.0	7.731254182608514E-6	0.0
5	0.0	2.319376254782554E-5	0.0	7.731254182608514E-6	0.0
6	0.0	2.319376254782554E-5	0.0	7.731254182608514E-6	0.0
7	0.0	2.319376254782554E-5	0.0	2.319376254782554E-5	0.0
8	0.0	4.638752509565108E-5	0.0	2.319376254782554E-5	0.0
9	7.731254182608514E-6	1.2370006692173622E-4	0.0	2.319376254782554E-5	0.0
10-11	7.731254182608514E-6	1.2370006692173622E-4	0.0	2.70593896391298E-5	0.0
12-13	7.731254182608514E-6	1.2370006692173622E-4	0.0	3.8656270913042566E-5	0.0
14-15	1.1596881273912771E-5	2.3193762547825538E-4	0.0	4.638752509565108E-5	0.0
16-17	3.0925016730434054E-5	2.551313880260809E-4	0.0	5.411877927825959E-5	0.0
18-19	4.638752509565108E-5	2.551313880260809E-4	0.0	8.117816891738938E-5	0.0
20-21	4.638752509565108E-5	2.5899701511738517E-4	0.0	1.0823755855651918E-4	0.0
22-23	5.0253152186955334E-5	3.247126756695575E-4	0.0	1.2756569401304045E-4	0.0
24-25	8.504379600869364E-5	3.749658278565129E-4	0.0	1.8941572747390855E-4	0.0
26-27	8.504379600869364E-5	4.097564716782512E-4	0.0	2.4353450675216817E-4	0.0
28-29	9.66406772826064E-5	4.1748772586085967E-4	0.0	5.527846740565087E-4	0.0
30-31	1.0823755855651918E-4	4.7547213223042355E-4	0.0	0.0014418789050564878	0.0
32-33	1.1596881273912769E-4	4.793377593217278E-4	0.0	0.003092501673043405	0.0
34-35	1.1983443983043195E-4	5.373221656912916E-4	0.0	0.005079433997973793	0.0
36-37	1.3143132110434472E-4	5.953065720608555E-4	0.0	0.0079013417746259	0.0
38-39	1.3143132110434472E-4	6.223659616999853E-4	0.0	0.012563288046738834	0.0
40-41	1.3916257528695324E-4	7.112753847999831E-4	0.0	0.01763499079053002	0.0
42-43	1.3916257528695324E-4	8.465723329956322E-4	0.0	0.02251341217975599	0.0
44-45	1.50759456560866E-4	9.818692811912812E-4	0.0	0.028103108953781944	0.0
46-47	1.5462508365217026E-4	9.818692811912812E-4	0.0	0.03441181236679049	0.0
48-49	1.5462508365217026E-4	9.818692811912812E-4	0.0	0.04034554995194252	0.0
50-51	1.584907107434745E-4	0.001086241212656496	0.0	0.04637979384146847	0.0
52-53	1.6235633783477876E-4	0.0010978380939304089	0.0	0.05225554702025094	0.0
54-55	1.6235633783477876E-4	0.0011017037210217131	0.0	0.05798054074247254	0.0
56-57	1.6622196492608303E-4	0.001113300602295626	0.0	0.06383696578579849	0.0
58-59	1.700875920173873E-4	0.0011210318564782345	0.0	0.06876564032721141	0.0
60-61	1.700875920173873E-4	0.0011519568732086683	0.0	0.07409247445902868	0.0
62-63	1.700875920173873E-4	0.0011712850086651897	0.0	0.07938065231993291	0.0
64-65	1.700875920173873E-4	0.0013645663632304026	0.0	0.08466883018083712	0.0
66-67	1.700875920173873E-4	0.0013761632445043154	0.0	0.0902778550903196	0.0
68-69	1.778188461999958E-4	0.0013877601257782281	0.0	0.09605310196472816	0.0
70-71	1.778188461999958E-4	0.0013993570070521407	0.0	0.10119052036907152	0.0
72-73	1.778188461999958E-4	0.001403222634143445	0.0	0.10578288535354098	0.0
74-75	1.778188461999958E-4	0.0014264163966912706	0.0	0.10979927190140609	0.0
76-77	1.778188461999958E-4	0.001461207040513009	0.0	0.11344842387559731	0.0
78-79	2.0101260874782134E-4	0.001476669548878226	0.0	0.11644428487135812	0.0
80-81	2.048782358391256E-4	0.001476669548878226	0.0	0.11772380743857982	0.0
82-83	2.0874386293042983E-4	0.0014805351759695302	0.0	0.11822633896044937	0.0
84-85	2.0874386293042983E-4	0.0014844008030608345	0.0	0.1186206329237624	0.0
86-87	2.0874386293042983E-4	0.0015153258197912686	0.0	0.11875979549904936	0.0
88-89	2.0874386293042983E-4	0.0015617133448869195	0.0	0.1187907205157798	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18960	0.0	46.70019	1
GTATCAA	33565	0.0	34.73394	1
CTTATAC	11490	0.0	33.162426	1
ATCAACG	39125	0.0	29.145168	3
TCAACGC	41215	0.0	27.698082	4
CAACGCA	41965	0.0	27.183313	5
TATCAAC	42385	0.0	27.126598	2
AACGCAG	42340	0.0	26.9526	6
ACGCAGA	43100	0.0	26.478153	7
TATACAC	17035	0.0	25.006351	3
CGCAGAG	49590	0.0	22.995897	8
TTATACA	18140	0.0	21.537563	2
ACATCTC	18055	0.0	20.890364	8
ACACATC	18450	0.0	20.716267	6
GCAGAGT	56765	0.0	20.029928	9
GAGTACT	33505	0.0	19.26011	12-13
GTACATG	38175	0.0	18.947933	1
TACATGG	37595	0.0	18.8245	2
ACATGGG	36435	0.0	18.762001	3
AGAGTAC	51285	0.0	18.67346	10-11
>>END_MODULE