FastQCFastQC Report
Fri 27 May 2016
SRR2139067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11076380
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150100.048.651321
CTTATAC114100.041.1676861
GTATCAA279400.036.8433271
TATACAC164400.031.557563
ATCAACG344050.029.4066523
TCAACGC346450.029.2151834
TTATACA167100.029.1006452
CAACGCA351650.028.8078165
TATCAAC356200.028.6136682
AACGCAG362300.028.0317526
ACGCAGA415950.024.3248167
CGCAGAG419350.024.1275948
TACACAT222300.023.166795
ACACATC205650.022.9594216
ACATCTC205950.022.8645958
CACATCT225950.020.7280777
GCAGAGT487350.020.5695639
ATACACA281550.019.6472074
GTACATG326250.019.6260621
TACATGG318150.019.5655772
Produced by FastQC (version 0.11.5)