FastQCFastQC Report
Fri 27 May 2016
SRR2139066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10929001
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141600.046.8475571
GTATCAA257400.036.386361
CTTATAC100650.034.342731
TCAACGC317150.028.9238934
ATCAACG317550.028.847453
CAACGCA323800.028.3039745
TATCAAC332850.027.7820052
AACGCAG333750.027.5619246
TATACAC147400.026.806943
ACGCAGA386850.023.7791427
CGCAGAG391850.023.4539958
TTATACA157050.023.1423662
ACATCTC165050.021.6572788
GTACATG301800.020.3520071
GCAGAGT453250.020.202029
ACACATC172750.020.0787586
TACATGG303300.019.9720232
TACACAT204200.019.2883115
ACATGGG308400.019.1430643
AGAGTAC416150.018.98042510-11
Produced by FastQC (version 0.11.5)