##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10929001
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47504213788616	34.0	31.0	34.0	31.0	34.0
2	32.61066688528988	34.0	31.0	34.0	31.0	34.0
3	32.66917186666924	34.0	31.0	34.0	31.0	34.0
4	36.19444686664408	37.0	35.0	37.0	35.0	37.0
5	36.11188131467826	37.0	35.0	37.0	35.0	37.0
6	36.14598589569166	37.0	36.0	37.0	35.0	37.0
7	36.09582056036046	37.0	36.0	37.0	35.0	37.0
8	36.06978808035611	37.0	35.0	37.0	35.0	37.0
9	37.783715821784625	39.0	38.0	39.0	35.0	39.0
10-11	37.711882449274185	39.0	37.5	39.0	35.0	39.0
12-13	37.65591795627066	39.0	37.0	39.0	35.0	39.0
14-15	38.995369155881676	40.0	38.0	41.0	35.5	41.0
16-17	38.9663749687643	40.0	38.0	41.0	35.0	41.0
18-19	38.96204589056218	40.0	38.0	41.0	35.0	41.0
20-21	38.93250816444176	40.0	38.0	41.0	35.0	41.0
22-23	38.82732294969576	40.0	38.0	41.0	35.0	41.0
24-25	38.69648746528412	40.0	38.0	41.0	34.0	41.0
26-27	38.62175014751169	40.0	38.0	41.0	34.5	41.0
28-29	38.562338804151395	40.0	38.0	41.0	34.0	41.0
30-31	38.48826816815931	40.0	38.0	41.0	34.0	41.0
32-33	38.38675770776287	40.0	38.0	41.0	34.0	41.0
34-35	38.282763370028874	40.0	38.0	41.0	34.0	41.0
36-37	38.42261046326258	40.0	38.0	41.0	34.0	41.0
38-39	38.48434186942846	40.0	38.0	41.0	34.0	41.0
40-41	38.42370439102993	40.0	38.0	41.0	34.0	41.0
42-43	38.391781339897065	40.0	38.0	41.0	34.0	41.0
44-45	38.36277883586283	40.0	38.0	41.0	34.0	41.0
46-47	38.27133890707862	40.0	38.0	41.0	33.0	41.0
48-49	38.19815283901833	40.0	38.0	41.0	33.0	41.0
50-51	38.12245630685573	40.0	37.5	41.0	33.0	41.0
52-53	38.05592122466779	40.0	37.0	41.0	33.0	41.0
54-55	37.93399628893332	40.0	37.0	41.0	33.0	41.0
56-57	37.77497265544457	40.0	36.5	41.0	33.0	41.0
58-59	37.654718518608426	39.5	36.0	41.0	33.0	41.0
60-61	37.50256845622552	39.0	36.0	41.0	32.5	41.0
62-63	37.287220274494345	39.0	35.5	41.0	32.0	41.0
64-65	36.97194602308376	38.5	35.0	40.0	31.5	41.0
66-67	36.68096443337123	38.0	35.0	40.0	31.0	41.0
68-69	36.409457613543054	37.5	35.0	40.0	31.0	41.0
70-71	36.10274398967674	37.0	35.0	39.5	31.0	41.0
72-73	35.793743409296255	36.5	34.5	39.0	31.0	41.0
74-75	35.46822112112688	36.0	34.0	39.0	30.5	40.0
76-77	34.487023161029086	35.0	33.5	37.0	29.5	39.0
78-79	34.805861966441256	35.0	34.0	37.0	30.0	39.0
80-81	34.649452873149514	35.0	34.0	37.0	30.0	39.0
82-83	34.40847593964577	35.0	34.0	36.5	30.0	39.0
84-85	34.146187801471065	35.0	34.0	36.0	30.0	37.5
86-87	33.91772841228872	35.0	34.0	36.0	30.0	37.0
88-89	33.70901588768233	35.0	34.0	35.0	30.0	37.0
90-91	33.53787078546554	35.0	33.0	35.0	29.5	36.0
92-93	33.406723433685556	35.0	33.0	35.0	29.0	36.0
94-95	33.346563322630786	35.0	33.0	35.0	29.0	36.0
96-97	33.36255769404073	35.0	33.0	35.0	30.0	35.5
98-99	33.40753909400179	35.0	33.0	35.0	30.0	35.0
100-101	31.929739808232988	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	9.0
20	51.0
21	393.0
22	1857.0
23	5989.0
24	14540.0
25	29718.0
26	51847.0
27	79835.0
28	113671.0
29	149935.0
30	190869.0
31	238294.0
32	297264.0
33	383116.0
34	520598.0
35	735843.0
36	1192369.0
37	2257896.0
38	3052800.0
39	1531437.0
40	80664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.23861286485497	21.72825510110715	15.638118766584316	24.395013267453564
2	13.492213629934295	21.720885940695368	41.77761975167632	23.009280677694022
3	16.74381919663281	26.756061853783514	31.65626315307896	24.843855796504712
4	11.793320523378169	19.018107786622746	38.95533900631348	30.233232683685607
5	12.558989843535548	38.23199743800897	36.19102388141642	13.017988837039072
6	29.27662183182359	38.91332235337177	18.68611034861378	13.123945466190868
7	25.65623570317504	33.2287125995059	23.366282368011714	17.748769329307347
8	23.956718459445653	37.49075510195305	21.2721089512207	17.280417487380596
9	24.972218412277574	16.944979692105434	21.606082751753796	36.47671914386319
10-11	23.5405656164905	27.262086236933797	29.31662299065968	19.880725155916025
12-13	24.427107402444484	24.730196058301104	29.437412499094712	21.405284040159707
14-15	21.53085242230851	26.31601292561549	27.044986566012103	25.1081480860639
16-17	21.096182533060432	29.537072967602434	27.696218529031153	21.670525970305977
18-19	21.10075294164581	28.517327430018536	29.608708060325	20.77321156801065
20-21	21.86185076612066	27.632349376605358	29.49257322348975	21.01322663378423
22-23	21.49758318166592	27.74804216461598	29.39898982342431	21.355384830293787
24-25	21.42573965230966	27.83762190842576	29.45455765399047	21.282080785274115
26-27	21.467463031813512	27.991974495994942	29.38426105116463	21.156301421026917
28-29	21.251744190910067	27.939306982472278	29.452858836800356	21.3560899898173
30-31	21.432697065071558	27.844676980080884	29.492073575272908	21.23055237957465
32-33	21.139704059822016	27.77021221925805	29.666016009675882	21.424067711244057
34-35	21.20312855342138	27.92843950867896	29.44570367511639	21.422728262783263
36-37	21.31572219752504	27.959165021899274	29.457264049241065	21.267848731334617
38-39	21.314540856704298	27.92582056412562	29.41810098299536	21.34153759617472
40-41	21.50954618260328	27.862212158203732	29.305661357450337	21.32258030174265
42-43	21.53687884785793	27.900779210201847	29.190565575206733	21.371776366733485
44-45	21.511897507316057	28.19807715628258	28.8548730243594	21.43515231204196
46-47	21.70055615972447	27.995744197545992	28.461182251479215	21.84251739125032
48-49	21.632066247714903	28.12472408420034	28.461220352079625	21.78198931600513
50-51	21.49759264935777	28.24122700728129	28.474379748828323	21.78680059453262
52-53	21.59136528649402	28.306040295817862	28.47280075338817	21.629793664299942
54-55	21.627869210285066	28.285143031761965	28.301941390342318	21.78504636761065
56-57	21.82706555948058	28.410936537458188	28.041355738353978	21.72064216470725
58-59	21.50162156640588	28.71908259509228	28.34106980651085	21.438226031990986
60-61	21.83030416524402	28.74483247570228	27.991922309788546	21.432941049265153
62-63	21.666099498218156	28.914906822140907	27.883901007950477	21.535092671690457
64-65	21.63532974087583	28.75122711046733	27.975300787680556	21.638142360976282
66-67	21.73193055847079	28.65116983422709	27.873616667462255	21.743282939839865
68-69	21.701032011006138	28.449284516545838	28.03276002254842	21.816923449899605
70-71	21.692858891341597	28.2411233978463	28.12116432045726	21.944853390354837
72-73	21.77703490171696	28.293564141256095	28.081000736009788	21.848400221017165
74-75	21.666907495880043	28.380785570275897	28.066127353559693	21.886179580284367
76-77	21.753542302660453	28.296613276438524	28.02922160842082	21.9206228124802
78-79	21.68161479388145	28.302286961023064	28.03334155697414	21.982756688121345
80-81	21.700841752717466	28.28581687504975	28.094121514573683	21.919219857659105
82-83	21.702538428424724	28.247132492354716	28.204357861698654	21.845971217521903
84-85	21.847260532710763	28.235526609497096	28.09525191096029	21.821960946831854
86-87	21.72322915239045	28.23956301316513	28.098242990199584	21.93896484424484
88-89	21.801662741866636	28.188901538354784	28.09162476808137	21.917810951697213
90-91	21.810862936761886	28.17447207897444	28.164335445500132	21.850329538763543
92-93	21.87105599026753	28.029133552939207	28.206435089777028	21.893375367016237
94-95	21.681971594260787	28.110992838103428	28.27451877061997	21.932516797015815
96-97	21.606825542593768	28.17205500406316	28.33021584569803	21.89090360764504
98-99	21.863333991679976	28.700518292625265	28.325017541650784	21.111130174043975
100-101	22.05356515650713	31.595158553124286	25.62429920820363	20.726977082164947
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2129.0
1	1957.5
2	2797.0
3	4043.5
4	5652.5
5	8861.0
6	11467.5
7	13926.0
8	15795.0
9	15756.0
10	14471.5
11	13295.0
12	14856.5
13	20584.0
14	34175.0
15	52518.5
16	66921.5
17	74309.0
18	74581.5
19	70246.0
20	64329.0
21	58846.0
22	56622.5
23	61137.5
24	72263.5
25	89868.0
26	112271.0
27	135564.5
28	160416.5
29	187849.5
30	214311.0
31	240630.5
32	267684.5
33	294438.0
34	321417.5
35	343927.0
36	366229.5
37	386782.0
38	399626.5
39	408621.0
40	418529.0
41	428589.5
42	432951.0
43	436079.0
44	442492.5
45	447926.5
46	452430.0
47	455986.0
48	461291.0
49	453544.5
50	432014.5
51	412896.0
52	388490.0
53	359739.0
54	332719.0
55	304544.0
56	276120.5
57	247108.5
58	216087.5
59	184591.0
60	152396.0
61	122253.0
62	95202.0
63	71679.5
64	52270.0
65	38460.0
66	29020.5
67	21948.0
68	16454.0
69	11787.0
70	8380.5
71	6034.5
72	4242.0
73	2825.5
74	1833.0
75	1234.5
76	851.0
77	553.5
78	349.5
79	228.0
80	149.5
81	102.0
82	74.0
83	53.0
84	37.5
85	29.5
86	24.0
87	18.5
88	14.0
89	9.0
90	5.5
91	3.5
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.149967137893024E-6
2	0.02320431666169671
3	9.149967137893024E-6
4	9.149967137893024E-6
5	9.149967137893024E-6
6	9.149967137893024E-6
7	9.149967137893024E-6
8	0.0
9	0.0
10-11	3.75148652653614E-4
12-13	0.0016973189040791561
14-15	4.574983568946512E-5
16-17	0.0
18-19	0.0
20-21	4.575138250383865E-6
22-23	0.0
24-25	0.008407007535964671
26-27	0.027015785761751534
28-29	0.33200361775967624
30-31	0.31692636193185003
32-33	0.31851264967442217
34-35	0.1711529398898205
36-37	0.030910170460212844
38-39	0.029792837766399835
40-41	0.01192488681205056
42-43	0.009645952448288697
44-45	0.00407066638715393
46-47	0.0024901479008173607
48-49	0.002271731151563384
50-51	0.0021102459211890874
52-53	5.590810370151722E-4
54-55	8.481492535139073E-5
56-57	4.029265157226711E-4
58-59	8.597137865279798E-4
60-61	0.003571479409033726
62-63	0.003644281382752488
64-65	0.003384474935969145
66-67	0.0064987894146401265
68-69	0.009898483581333696
70-71	0.01372123546764932
72-73	0.013159544594077205
74-75	0.00999669737094075
76-77	0.0047529797310947005
78-79	0.0020090478662464465
80-81	0.0018377790164918844
82-83	3.19733237839673E-4
84-85	2.9424957472108964E-5
86-87	0.0
88-89	5.742397383207647E-4
90-91	0.002165952853280869
92-93	0.013786347774496254
94-95	4.504476783768048E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	218.0
20-21	766.0
22-23	1639.0
24-25	3802.0
26-27	6037.0
28-29	9986.0
30-31	22689.0
32-33	29425.0
34-35	43300.0
36-37	59407.0
38-39	71026.0
40-41	74990.0
42-43	89916.0
44-45	107718.0
46-47	105366.0
48-49	93250.0
50-51	83427.0
52-53	81973.0
54-55	91615.0
56-57	99153.0
58-59	98635.0
60-61	98890.0
62-63	100716.0
64-65	107953.0
66-67	115464.0
68-69	105720.0
70-71	98594.0
72-73	95262.0
74-75	97710.0
76-77	98598.0
78-79	103127.0
80-81	105621.0
82-83	104484.0
84-85	107368.0
86-87	113494.0
88-89	121219.0
90-91	128970.0
92-93	133574.0
94-95	152293.0
96-97	228157.0
98-99	935824.0
100-101	6601625.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.97733584881777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.99547130614216	72.13634304438624
2	7.84768223095157	12.866641637630327
3	2.2119529883498843	5.439900390232637
4	0.8565065444978459	2.8085649862004116
5	0.42497280772226825	1.741906929263172
6	0.23096434599483648	1.1360305056432756
7	0.13039940455892082	0.7482857047408759
8	0.07770368774858434	0.5095953045805891
9	0.05369695024134624	0.3961739631592887
>10	0.16825736220650817	2.036363402444528
>50	0.0019809638046139636	0.10603182290692546
>100	3.7144106368812923E-4	0.04955706230177592
>500	3.9966717742784575E-5	0.024605246509880233
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.4899802827358145E-5	0.0	0.0	0.0
3	0.0	5.4899802827358145E-5	0.0	0.0	0.0
4	0.0	1.0979960565471629E-4	0.0	0.0	0.0
5	0.0	1.1894957279260931E-4	0.0	0.0	0.0
6	0.0	1.1894957279260931E-4	0.0	0.0	0.0
7	0.0	1.1894957279260931E-4	0.0	0.0	0.0
8	0.0	1.555494413441814E-4	0.0	0.0	0.0
9	0.0	2.653490469988977E-4	0.0	9.149967137893024E-6	0.0
10-11	9.149967137893024E-6	2.653490469988977E-4	0.0	3.6599868551572095E-5	0.0
12-13	9.149967137893024E-6	2.699240305678442E-4	0.0	3.6599868551572095E-5	0.0
14-15	9.149967137893024E-6	3.1567386625730936E-4	0.0	4.1174852120518605E-5	0.0
16-17	9.149967137893024E-6	3.3397380053309543E-4	0.0	6.404976996525117E-5	0.0
18-19	9.149967137893024E-6	3.6142370194677445E-4	0.0	7.319973710314419E-5	0.0
20-21	9.149967137893024E-6	3.9344858692940007E-4	0.0	7.319973710314419E-5	0.0
22-23	9.149967137893024E-6	4.254734719120256E-4	0.0	1.0522462208576979E-4	0.0
24-25	9.149967137893024E-6	4.3919842261886516E-4	0.0	1.3267452349944887E-4	0.0
26-27	9.149967137893024E-6	4.4834838975675817E-4	0.0	1.7842435918891396E-4	0.0
28-29	9.149967137893024E-6	4.529233733257047E-4	0.0	4.02598554067293E-4	0.0
30-31	9.149967137893024E-6	5.53573011842528E-4	0.0	0.0011986456950639863	0.0
32-33	9.149967137893024E-6	5.672979625493675E-4	0.0	0.002982889286953126	0.0
34-35	9.149967137893024E-6	6.450726832214581E-4	0.0	0.005005032024427484	0.0
36-37	9.149967137893024E-6	7.228474038935489E-4	0.0	0.007955896426397985	0.0
38-39	9.149967137893024E-6	7.411473381693349E-4	0.0	0.012622379666723426	0.0
40-41	9.149967137893024E-6	7.868971738588001E-4	0.0	0.017805836050339825	0.0
42-43	1.8299934275786047E-5	8.509469438240512E-4	0.0	0.022879492828301505	0.0
44-45	1.8299934275786047E-5	9.790464837545537E-4	0.0	0.028932196090017744	0.0
46-47	1.8299934275786047E-5	0.0010522462208576978	0.0	0.03526397334943972	0.0
48-49	1.8299934275786047E-5	0.0010522462208576978	0.0	0.04151340090462065	0.0
50-51	1.8299934275786047E-5	0.0012535454978913445	0.0	0.04757525413347478	0.0
52-53	1.8299934275786047E-5	0.0012718454321671304	0.0	0.053856706573638335	0.0
54-55	1.8299934275786047E-5	0.0012947203500118629	0.0	0.060215933734473995	0.0
56-57	1.8299934275786047E-5	0.0013358952021323815	0.0	0.06606276273558764	0.0
58-59	1.8299934275786047E-5	0.0013450451692702747	0.0	0.07206056619447651	0.0
60-61	1.8299934275786047E-5	0.001349620152839221	0.0	0.07746362178940235	0.0
62-63	1.8299934275786047E-5	0.0013770700542529001	0.0	0.08247780378096772	0.0
64-65	1.8299934275786047E-5	0.0016286941505449584	0.0	0.0876887100659978	0.0
66-67	1.8299934275786047E-5	0.001633269134113905	0.0	0.09359501385350774	0.0
68-69	1.8299934275786047E-5	0.0016561440519586375	0.0	0.0989797695141578	0.0
70-71	1.8299934275786047E-5	0.0016744439862344234	0.0	0.104350800224101	0.0
72-73	1.8299934275786047E-5	0.001688168936941263	0.0	0.1092002828071843	0.0
74-75	1.8299934275786047E-5	0.0017293437890617816	0.0	0.11308901884078884	0.0
76-77	1.8299934275786047E-5	0.0018116934933028188	0.0	0.11687710523587655	0.0
78-79	1.8299934275786047E-5	0.001866593296130177	0.0	0.12006586878343226	0.0
80-81	1.8299934275786047E-5	0.001866593296130177	0.0	0.12143836385411622	0.0
82-83	1.8299934275786047E-5	0.0018757432632680701	0.0	0.12200566181666558	0.0
84-85	1.8299934275786047E-5	0.001903193164681749	0.0	0.12233963561719868	0.0
86-87	1.8299934275786047E-5	0.0019260680825264817	0.0	0.12247688512426708	0.0
88-89	1.8299934275786047E-5	0.0020404426717501446	0.0	0.12254550987780127	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14160	0.0	46.847557	1
GTATCAA	25740	0.0	36.38636	1
CTTATAC	10065	0.0	34.34273	1
TCAACGC	31715	0.0	28.923893	4
ATCAACG	31755	0.0	28.84745	3
CAACGCA	32380	0.0	28.303974	5
TATCAAC	33285	0.0	27.782005	2
AACGCAG	33375	0.0	27.561924	6
TATACAC	14740	0.0	26.80694	3
ACGCAGA	38685	0.0	23.779142	7
CGCAGAG	39185	0.0	23.453995	8
TTATACA	15705	0.0	23.142366	2
ACATCTC	16505	0.0	21.657278	8
GTACATG	30180	0.0	20.352007	1
GCAGAGT	45325	0.0	20.20202	9
ACACATC	17275	0.0	20.078758	6
TACATGG	30330	0.0	19.972023	2
TACACAT	20420	0.0	19.288311	5
ACATGGG	30840	0.0	19.143064	3
AGAGTAC	41615	0.0	18.980425	10-11
>>END_MODULE