FastQCFastQC Report
Fri 27 May 2016
SRR2139065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8816065
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117950.050.075611
GTATCAA211750.037.1577951
TCAACGC268300.028.8311984
ATCAACG268750.028.814453
CAACGCA273750.028.2573685
TATCAAC278200.027.9473322
AACGCAG281950.027.525866
ACGCAGA326700.023.7303567
CGCAGAG337200.023.016558
GTACATG258900.021.2096941
TACATGG261800.020.3435652
GCAGAGT380700.020.1751739
ACATGGG265050.019.547053
GAGTACT238950.019.3515312-13
AGAGTAC348150.018.7516110-11
GTGGTAT57400.018.7492221
CAGAGTA373950.017.97334310-11
CATGGGG196150.017.4288124
GTACTTT265450.017.3398614-15
AGTACTT261650.017.21119912-13
Produced by FastQC (version 0.11.5)