##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8816065
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45567574649234	34.0	31.0	34.0	31.0	34.0
2	32.58549171313959	34.0	31.0	34.0	31.0	34.0
3	32.64335358235222	34.0	31.0	34.0	31.0	34.0
4	36.178369261115925	37.0	35.0	37.0	35.0	37.0
5	36.094958124741595	37.0	35.0	37.0	35.0	37.0
6	36.13172906506474	37.0	36.0	37.0	35.0	37.0
7	36.081049311682705	37.0	35.0	37.0	35.0	37.0
8	36.0555367956112	37.0	35.0	37.0	35.0	37.0
9	37.76400854576276	39.0	38.0	39.0	35.0	39.0
10-11	37.6926577220109	39.0	37.0	39.0	35.0	39.0
12-13	37.63417828702488	39.0	37.0	39.0	35.0	39.0
14-15	38.963048933963165	40.0	38.0	41.0	35.0	41.0
16-17	38.93597892030061	40.0	38.0	41.0	35.0	41.0
18-19	38.935928897983395	40.0	38.0	41.0	35.0	41.0
20-21	38.90452971473124	40.0	38.0	41.0	35.0	41.0
22-23	38.80104528452951	40.0	38.0	41.0	35.0	41.0
24-25	38.67368862846526	40.0	38.0	41.0	34.0	41.0
26-27	38.59815543139558	40.0	38.0	41.0	34.0	41.0
28-29	38.53914783241506	40.0	38.0	41.0	34.0	41.0
30-31	38.4673901360284	40.0	38.0	41.0	34.0	41.0
32-33	38.35752727484194	40.0	38.0	41.0	34.0	41.0
34-35	38.24505323088665	40.0	38.0	41.0	34.0	41.0
36-37	38.388954980087306	40.0	38.0	41.0	34.0	41.0
38-39	38.45872163764505	40.0	38.0	41.0	34.0	41.0
40-41	38.38828189004944	40.0	38.0	41.0	34.0	41.0
42-43	38.35033447197739	40.0	38.0	41.0	34.0	41.0
44-45	38.31540786177504	40.0	38.0	41.0	33.5	41.0
46-47	38.220721300080946	40.0	38.0	41.0	33.0	41.0
48-49	38.14108806030601	40.0	37.5	41.0	33.0	41.0
50-51	38.0699978723542	40.0	37.0	41.0	33.0	41.0
52-53	37.993564653746645	40.0	37.0	41.0	33.0	41.0
54-55	37.8642336838231	40.0	37.0	41.0	33.0	41.0
56-57	37.70329786605256	40.0	36.0	41.0	33.0	41.0
58-59	37.567806450857574	39.0	36.0	41.0	33.0	41.0
60-61	37.41217859288843	39.0	36.0	41.0	32.0	41.0
62-63	37.18427849670532	39.0	35.0	40.5	32.0	41.0
64-65	36.8484305104074	38.0	35.0	40.0	31.5	41.0
66-67	36.54375085877906	38.0	35.0	40.0	31.0	41.0
68-69	36.257193033364004	37.0	35.0	40.0	31.0	41.0
70-71	35.94511668258393	37.0	35.0	39.0	31.0	41.0
72-73	35.63477212890302	36.0	34.0	39.0	31.0	41.0
74-75	35.311528619862244	36.0	34.0	39.0	30.0	40.0
76-77	34.33762032273022	35.0	33.0	37.0	29.0	39.0
78-79	34.662326018140874	35.0	34.0	37.0	30.0	39.0
80-81	34.51662140269269	35.0	34.0	37.0	30.0	39.0
82-83	34.287859833386996	35.0	34.0	36.0	30.0	38.5
84-85	34.037865183975356	35.0	34.0	36.0	30.0	37.0
86-87	33.82031406394411	35.0	34.0	35.5	30.0	37.0
88-89	33.62573720214064	35.0	33.0	35.0	29.5	36.5
90-91	33.465024362676914	35.0	33.0	35.0	29.0	36.0
92-93	33.34042495227891	35.0	33.0	35.0	29.0	36.0
94-95	33.28597735136337	35.0	33.0	35.0	29.0	36.0
96-97	33.30911164870345	35.0	33.0	35.0	29.5	35.0
98-99	33.357074705779496	35.0	33.0	35.0	30.0	35.0
100-101	31.900274636581827	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	3.0
19	12.0
20	47.0
21	305.0
22	1328.0
23	4471.0
24	11426.0
25	23694.0
26	41548.0
27	64764.0
28	93046.0
29	123361.0
30	157835.0
31	197485.0
32	246337.0
33	318328.0
34	433933.0
35	615688.0
36	1001171.0
37	1868243.0
38	2385629.0
39	1167521.0
40	59887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.994830004011995	21.33871351389243	15.391193413929418	24.275263068166158
2	13.80534132116431	21.676446101995513	41.372253058007004	23.145959518833177
3	17.136653530794135	26.441340816341807	31.251924044998773	25.17008160786528
4	12.061382962143414	18.588140600644596	38.73911050081091	30.61136593640108
5	12.897188438237894	38.02641565206956	35.80371097704519	13.272684932647358
6	29.836340742197677	38.455212594377464	18.504545283885854	13.203901379539001
7	26.15332106330468	33.021103980439335	23.181642778828007	17.64393217742797
8	24.26542907748525	37.267942103421426	20.94333469637531	17.523294122718017
9	25.3029441139556	16.845327252010957	21.436581967124788	36.41514666690865
10-11	23.949657612326636	27.06540812587979	28.991819447075386	19.99311481471819
12-13	24.783927801514512	24.617807854178334	29.22182020955501	21.376444134752145
14-15	21.821992527843353	26.031536187498673	26.95981908022998	25.186652204427993
16-17	21.33751849606372	29.231834157302604	27.58373492028473	21.846912426348943
18-19	21.3646677968005	28.27225071502989	29.51952486738698	20.84355662078263
20-21	22.142045935346406	27.33362735149304	29.508312478688786	21.01601423447177
22-23	21.87673018047337	27.318021386810287	29.338375867305917	21.46687256541043
24-25	21.701165025297158	27.475441870440655	29.38597441857958	21.43741868568261
26-27	21.6221189209075	27.592508484699156	29.351779203658896	21.433593390734448
28-29	21.544213267313623	27.65223135055767	29.32088800676213	21.482667375366578
30-31	21.813372189266147	27.506451238629833	29.244540818637336	21.43563575346668
32-33	21.55485761150485	27.60355507429369	29.24845871218174	21.593128602019718
34-35	21.679910058541655	27.653551311043117	29.215702375038106	21.45083625537712
36-37	21.728364615491262	27.65517661625695	29.117180319609894	21.499278448641896
38-39	21.604283247025446	27.711955832178365	29.127664029474797	21.556096891321396
40-41	21.849193667952743	27.59803676080073	29.002611413149847	21.550158158096675
42-43	21.789158023014682	27.735528040779773	28.75456521010456	21.72074872610099
44-45	21.835471128076613	27.887794058275194	28.532317011761947	21.744417801886247
46-47	21.954367739326468	27.863593563539133	28.215322665189497	21.9667160319449
48-49	21.91702136793758	28.033197780720194	28.101705699857664	21.94807515148456
50-51	21.904936680486916	28.07322375226126	28.041602227910033	21.980237339341787
52-53	22.05555697876714	28.00975000274915	27.947468625710243	21.98722439277347
54-55	22.03103532169348	27.98542260632246	27.94206759775647	22.04147447422759
56-57	22.06291166977688	28.101862526740245	27.844913563414085	21.990312240068782
58-59	21.928508862230505	28.183213397531926	27.94902016277203	21.939257577465543
60-61	22.07801212122757	28.15016659439133	27.852676837469065	21.919144446912036
62-63	22.064925544292404	28.219892104925403	27.819263044023007	21.895919306759186
64-65	22.03099172439522	28.177157236100946	27.787900845038067	22.003950194465766
66-67	22.095816952880845	28.150466982872864	27.645932517209857	22.107783547036433
68-69	22.01689162644321	28.078322279894678	27.804041405413184	22.100744688248927
70-71	21.979834579125658	27.95018782928557	27.82853468441107	22.241442907177703
72-73	22.11345867272572	27.96593942006324	27.75087943590076	22.169722471310283
74-75	21.993972187384013	28.040716530878314	27.73122603116651	22.234085250571162
76-77	22.080926035109893	27.9638244802464	27.687644318156103	22.267605166487606
78-79	22.00992429324066	27.970483296311404	27.722649837699	22.296942572748936
80-81	22.06125118051592	27.935229666333093	27.761013811858536	22.24250534129245
82-83	22.073444459146803	27.926505146488502	27.82634994624361	22.17370044812109
84-85	22.18226020181537	27.865450003575802	27.76111377322077	22.191176021388053
86-87	22.04797760765259	27.886168195828443	27.82655592819994	22.239298268319025
88-89	22.11013873515998	27.787171351065084	27.829888257189197	22.27280165658575
90-91	22.14837699229747	27.826242646600598	27.82590023429372	22.19948012680821
92-93	22.175998353550312	27.716618774168687	27.89467983207451	22.21270304020649
94-95	21.966713042059325	27.773825220159736	27.97420597825171	22.285255759529228
96-97	21.972201064667463	27.826673449955706	27.958011183416854	22.24311430195998
98-99	22.24543126541934	28.415694787982787	27.983506129026324	21.355367817571548
100-101	22.489994584647423	31.231897696722875	25.227340244472945	21.050767474156757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2272.0
1	1924.5
2	2463.0
3	3558.5
4	4754.5
5	7091.0
6	9093.5
7	11320.5
8	13359.0
9	13770.5
10	12813.0
11	11721.5
12	12715.0
13	17054.5
14	28095.5
15	43032.5
16	54428.0
17	60199.0
18	60741.0
19	57558.0
20	52979.5
21	48848.5
22	46870.0
23	49789.5
24	57546.5
25	69736.0
26	85333.5
27	102730.5
28	122532.5
29	143145.5
30	162733.0
31	182406.0
32	203219.5
33	223483.0
34	242252.5
35	259149.5
36	277593.0
37	295502.5
38	309137.0
39	319343.0
40	327367.5
41	335326.0
42	340649.0
43	344834.5
44	350210.5
45	354847.0
46	358980.5
47	361473.0
48	360596.5
49	356010.0
50	347979.0
51	337284.5
52	321042.5
53	301649.5
54	284039.5
55	264855.5
56	242668.0
57	219234.0
58	194657.0
59	168028.0
60	140522.0
61	115428.5
62	91787.0
63	70666.0
64	53840.5
65	40291.0
66	30310.0
67	22876.0
68	16829.5
69	12102.5
70	8481.0
71	6026.5
72	4120.0
73	2699.5
74	1832.5
75	1212.0
76	803.0
77	503.5
78	332.0
79	218.0
80	147.5
81	105.0
82	76.0
83	52.5
84	37.5
85	29.5
86	20.5
87	13.0
88	10.0
89	7.5
90	7.0
91	7.0
92	5.5
93	3.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.537171629292661E-5
2	0.022583771784804217
3	3.4028787219694955E-5
4	3.4028787219694955E-5
5	4.537171629292661E-5
6	4.537171629292661E-5
7	3.4028787219694955E-5
8	0.0
9	0.0
10-11	3.970025175631078E-4
12-13	0.0016560676446918212
14-15	2.835732268307913E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008147080446065137
26-27	0.026698808157030764
28-29	0.32836339336555553
30-31	0.31426686975993245
32-33	0.31589804544317823
34-35	0.167751386557103
36-37	0.027261446979959334
38-39	0.025681237274184462
40-41	0.010061452209526893
42-43	0.007336144975350085
44-45	0.0022471576560276958
46-47	0.001804993736313127
48-49	0.0016544032516148696
50-51	0.0019313737221556225
52-53	2.027304473855513E-4
54-55	6.19533891198821E-5
56-57	2.938480166790635E-4
58-59	3.4082715468402544E-4
60-61	0.0021921091843703383
62-63	0.001190170606131373
64-65	0.0013903909045045093
66-67	0.006580536938977211
68-69	0.00997502855866615
70-71	0.013954615935795337
72-73	0.013476778763366707
74-75	0.010714017545181788
76-77	0.005122411759169464
78-79	0.001966224752971949
80-81	0.0019621559828695255
82-83	3.0236527395301703E-4
84-85	4.374071466641472E-5
86-87	0.0
88-89	7.342968006688394E-4
90-91	0.0024729166172083343
92-93	0.014721487329734937
94-95	3.701407314437623E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	172.0
20-21	612.0
22-23	1378.0
24-25	3483.0
26-27	5036.0
28-29	8196.0
30-31	18531.0
32-33	24168.0
34-35	34922.0
36-37	48806.0
38-39	58544.0
40-41	62023.0
42-43	73628.0
44-45	88424.0
46-47	87383.0
48-49	76798.0
50-51	68407.0
52-53	67242.0
54-55	72048.0
56-57	75496.0
58-59	75505.0
60-61	74860.0
62-63	75121.0
64-65	80421.0
66-67	85196.0
68-69	83484.0
70-71	81856.0
72-73	79488.0
74-75	80938.0
76-77	82348.0
78-79	86215.0
80-81	88433.0
82-83	86354.0
84-85	88639.0
86-87	94174.0
88-89	100255.0
90-91	107089.0
92-93	110867.0
94-95	126042.0
96-97	188462.0
98-99	758380.0
100-101	5306641.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.50336666510194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.7995445341405	76.78163382661765
2	7.175150696192609	12.26999081707836
3	1.7414092702093487	4.466890660341525
4	0.5972717170383083	2.042749704824859
5	0.27032690822910516	1.1556930376878267
6	0.15294377965153735	0.7846324842415172
7	0.08005935601895321	0.47917411308623437
8	0.045003145963341484	0.3078336392309351
9	0.03356191992298929	0.25826914306441284
>10	0.10267725274416785	1.3121449353313637
>50	0.0018533906930491718	0.10337266622914458
>100	1.9802919615374257E-4	0.03761497226619445
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1342929073231652E-5	0.0
4	0.0	1.1342929073231652E-5	0.0	1.1342929073231652E-5	0.0
5	0.0	1.1342929073231652E-5	0.0	1.1342929073231652E-5	0.0
6	0.0	1.1342929073231652E-5	0.0	1.1342929073231652E-5	0.0
7	0.0	1.1342929073231652E-5	0.0	1.1342929073231652E-5	0.0
8	0.0	2.2685858146463304E-5	0.0	1.1342929073231652E-5	0.0
9	0.0	4.537171629292661E-5	0.0	1.1342929073231652E-5	2.2685858146463304E-5
10-11	0.0	4.537171629292661E-5	0.0	2.2685858146463304E-5	2.2685858146463304E-5
12-13	0.0	4.537171629292661E-5	0.0	2.2685858146463304E-5	2.2685858146463304E-5
14-15	0.0	7.372903897600574E-5	0.0	2.835732268307913E-5	3.4028787219694955E-5
16-17	0.0	7.940050351262156E-5	0.0	3.4028787219694955E-5	3.4028787219694955E-5
18-19	0.0	1.1342929073231652E-4	0.0	3.970025175631078E-5	3.4028787219694955E-5
20-21	0.0	1.4745807795201146E-4	0.0	4.537171629292661E-5	3.4028787219694955E-5
22-23	0.0	1.4745807795201146E-4	0.0	6.238610990277408E-5	3.4028787219694955E-5
24-25	0.0	1.4745807795201146E-4	0.0	7.372903897600574E-5	3.4028787219694955E-5
26-27	0.0	1.6447247156185893E-4	0.0	9.641489712246905E-5	3.4028787219694955E-5
28-29	0.0	1.758154006350906E-4	0.0	3.7431665941664447E-4	3.4028787219694955E-5
30-31	0.0	2.211871169280172E-4	0.0	0.0011342929073231652	3.4028787219694955E-5
32-33	0.0	2.3820151053786467E-4	0.0	0.0026769312612826696	4.537171629292661E-5
34-35	0.0	2.835732268307913E-4	0.0	0.004707315565391135	5.671464536615826E-5
36-37	0.0	3.6297373034341286E-4	0.0	0.00786632131228615	5.671464536615826E-5
38-39	0.0	3.8565958848987614E-4	0.0	0.0125793083422139	5.671464536615826E-5
40-41	0.0	4.1968837570957113E-4	0.0	0.017711983747851226	5.671464536615826E-5
42-43	0.0	4.3103130478280274E-4	0.0	0.02341180560715013	5.671464536615826E-5
44-45	0.0	4.6506009200249774E-4	0.0	0.029463258267719214	5.671464536615826E-5
46-47	0.0	4.87745950148961E-4	0.0	0.0364108023250736	5.671464536615826E-5
48-49	0.0	4.934174146855768E-4	0.0	0.0437213201127714	5.671464536615826E-5
50-51	0.0	5.501320600517351E-4	0.0	0.05046469144680762	5.671464536615826E-5
52-53	0.0	5.671464536615826E-4	0.0	0.057054933238355206	5.671464536615826E-5
54-55	0.0	5.671464536615826E-4	0.0	0.0637132326043422	5.671464536615826E-5
56-57	0.0	5.671464536615826E-4	0.0	0.06963991304510572	5.671464536615826E-5
58-59	0.0	5.728179181981985E-4	0.0	0.07538510662069756	5.671464536615826E-5
60-61	0.0	5.784893827348142E-4	0.0	0.0813117870614611	5.671464536615826E-5
62-63	0.0	5.784893827348142E-4	0.0	0.08740293997378648	5.671464536615826E-5
64-65	0.0	6.465469571742042E-4	0.0	0.09314813354937833	5.671464536615826E-5
66-67	0.0	6.578898862474358E-4	0.0	0.09914287156458125	5.671464536615826E-5
68-69	0.0	6.635613507840516E-4	0.0	0.10504119468266171	5.671464536615826E-5
70-71	0.0	6.692328153206674E-4	0.0	0.1105595296767889	6.238610990277408E-5
72-73	0.0	6.692328153206674E-4	0.0	0.11512505862876465	7.940050351262156E-5
74-75	0.0	6.805757443938991E-4	0.0	0.11899299744273664	7.940050351262156E-5
76-77	0.0	7.089330670769782E-4	0.0	0.1226681064624637	7.940050351262156E-5
78-79	0.0	7.14604531613594E-4	0.0	0.12581009781574887	8.507196804923738E-5
80-81	0.0	7.14604531613594E-4	0.0	0.1271825922336099	9.074343258585321E-5
82-83	0.0	7.14604531613594E-4	0.0	0.12785182504893056	9.074343258585321E-5
84-85	0.0	7.14604531613594E-4	0.0	0.1282941992827866	9.074343258585321E-5
86-87	0.0	7.14604531613594E-4	0.0	0.12842464296712877	9.074343258585321E-5
88-89	0.0	7.259474606868257E-4	0.0	0.12845867175434844	9.074343258585321E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11795	0.0	50.07561	1
GTATCAA	21175	0.0	37.157795	1
TCAACGC	26830	0.0	28.831198	4
ATCAACG	26875	0.0	28.81445	3
CAACGCA	27375	0.0	28.257368	5
TATCAAC	27820	0.0	27.947332	2
AACGCAG	28195	0.0	27.52586	6
ACGCAGA	32670	0.0	23.730356	7
CGCAGAG	33720	0.0	23.01655	8
GTACATG	25890	0.0	21.209694	1
TACATGG	26180	0.0	20.343565	2
GCAGAGT	38070	0.0	20.175173	9
ACATGGG	26505	0.0	19.54705	3
GAGTACT	23895	0.0	19.35153	12-13
AGAGTAC	34815	0.0	18.75161	10-11
GTGGTAT	5740	0.0	18.749222	1
CAGAGTA	37395	0.0	17.973343	10-11
CATGGGG	19615	0.0	17.428812	4
GTACTTT	26545	0.0	17.33986	14-15
AGTACTT	26165	0.0	17.211199	12-13
>>END_MODULE