FastQCFastQC Report
Fri 27 May 2016
SRR2139064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11190894
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152200.049.361961
CTTATAC105800.039.888011
GTATCAA284450.037.808331
ATCAACG355800.029.6820583
TCAACGC357950.029.4681284
TATCAAC365000.029.196532
CAACGCA362950.029.0270165
TATACAC167350.028.5669443
AACGCAG376400.028.114596
TTATACA170800.026.0780542
ACGCAGA433450.024.2874957
CGCAGAG439900.023.9217118
ACATCTC200600.021.8612128
ACACATC203050.021.3662436
TACACAT221900.021.3334485
GCAGAGT500900.020.8216889
CACATCT215700.019.8575577
GAGTACT299150.019.47933812-13
GTACATG344150.019.246741
TACATGG338750.018.975862
Produced by FastQC (version 0.11.5)