FastQCFastQC Report
Fri 27 May 2016
SRR2139063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28619607
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA333200.044.6024171
GTATCAA623600.036.4803851
CTTATAC261700.035.7398031
ATCAACG757200.031.1812763
TCAACGC763300.030.9377374
CAACGCA777050.030.36815
TATCAAC802000.029.6929952
AACGCAG812500.029.1484456
TATACAC417000.026.2944153
CGCAGAG946050.024.9835228
ACGCAGA947400.024.9615697
TTATACA409850.024.4613762
GCAGAGT1115800.021.1324889
ACATCTC470900.020.6572848
ACACATC485550.019.7500026
TACACAT561300.019.2504885
AGAGTAC1021150.018.9444510-11
GAGTACT698550.018.48479512-13
ATCTCCG318900.018.0425157
TATGCCG307950.017.96502742-43
Produced by FastQC (version 0.11.5)