##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28619607
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.004788116063228	28.0	18.0	32.0	18.0	33.0
2	29.5734699990814	32.0	27.0	33.0	25.0	33.0
3	31.56155372783421	33.0	32.0	33.0	27.0	33.0
4	31.177051313108528	33.0	31.0	33.0	28.0	33.0
5	32.3818418261299	33.0	33.0	33.0	32.0	34.0
6	35.89946231616668	38.0	36.0	38.0	31.0	38.0
7	36.721629650609806	38.0	37.0	38.0	34.0	38.0
8	36.91417988374194	38.0	38.0	38.0	35.0	38.0
9	36.987735191472055	38.0	38.0	38.0	35.0	38.0
10-11	37.08422591197706	38.0	38.0	38.0	36.0	38.0
12-13	37.11658699576133	38.0	38.0	38.0	36.0	38.0
14-15	37.12122007824915	38.0	38.0	38.0	36.0	38.0
16-17	37.092484865358216	38.0	38.0	38.0	36.0	38.0
18-19	37.11938797761968	38.0	38.0	38.0	36.0	38.0
20-21	37.139568368786136	38.0	38.0	38.0	36.0	38.0
22-23	37.13758720398497	38.0	38.0	38.0	36.0	38.0
24-25	37.13565643549627	38.0	38.0	38.0	36.0	38.0
26-27	37.10522862112309	38.0	38.0	38.0	36.0	38.0
28-29	37.07959303425141	38.0	38.0	38.0	36.0	38.0
30-31	37.07741315687795	38.0	38.0	38.0	36.0	38.0
32-33	37.063435309962074	38.0	38.0	38.0	36.0	38.0
34-35	37.034356300190815	38.0	38.0	38.0	36.0	38.0
36-37	37.01627507230839	38.0	38.0	38.0	36.0	38.0
38-39	36.97595185107036	38.0	38.0	38.0	36.0	38.0
40-41	36.97778386134628	38.0	38.0	38.0	36.0	38.0
42-43	36.95143680952724	38.0	38.0	38.0	36.0	38.0
44-45	36.89731917228013	38.0	38.0	38.0	35.0	38.0
46-47	36.870012785779195	38.0	38.0	38.0	35.0	38.0
48-49	36.83424070146842	38.0	38.0	38.0	35.0	38.0
50-51	36.782820878929755	38.0	38.0	38.0	35.0	38.0
52-53	36.748593529018464	38.0	38.0	38.0	35.0	38.0
54-55	36.706191355256486	38.0	38.0	38.0	35.0	38.0
56-57	36.696384773089584	38.0	38.0	38.0	35.0	38.0
58-59	36.668025055929675	38.0	38.0	38.0	34.0	38.0
60-61	36.66808200451993	38.0	38.0	38.0	34.0	38.0
62-63	36.642484700328524	38.0	38.0	38.0	34.0	38.0
64-65	36.62440974687001	38.0	38.0	38.0	34.0	38.0
66-67	36.604267578156055	38.0	38.0	38.0	34.0	38.0
68-69	36.59612157624025	38.0	38.0	38.0	34.0	38.0
70-71	36.565824365283184	38.0	38.0	38.0	34.0	38.0
72-73	36.537081111857574	38.0	38.0	38.0	34.0	38.0
74-75	36.54966116049836	38.0	38.0	38.0	34.0	38.0
76-77	36.517142588425756	38.0	38.0	38.0	34.0	38.0
78-79	36.4844413214761	38.0	38.0	38.0	34.0	38.0
80-81	36.47179869683012	38.0	38.0	38.0	34.0	38.0
82-83	36.40545774963883	38.0	38.0	38.0	34.0	38.0
84-85	36.397479727707044	38.0	37.5	38.0	34.0	38.0
86-87	36.373245743706704	38.0	37.0	38.0	34.0	38.0
88-89	36.33853908051485	38.0	37.0	38.0	34.0	38.0
90-91	36.30844160078611	38.0	37.0	38.0	34.0	38.0
92-93	36.305348091416505	38.0	37.0	38.0	33.5	38.0
94-95	36.24292575607748	38.0	37.0	38.0	33.0	38.0
96-97	36.20494361286613	38.0	37.0	38.0	33.0	38.0
98-99	36.23060168131768	38.0	37.0	38.0	33.0	38.0
100	34.27850002068845	38.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	39.0
18	125.0
19	311.0
20	599.0
21	1418.0
22	3981.0
23	10295.0
24	23878.0
25	46141.0
26	79831.0
27	127289.0
28	191454.0
29	271155.0
30	364814.0
31	474327.0
32	620192.0
33	837187.0
34	1226971.0
35	2099715.0
36	5500762.0
37	1.6739123E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.22688110287468	25.06189899812391	14.645001938705867	24.066217960295543
2	12.85399735543044	22.72486965316249	41.62189573888296	22.799237252524108
3	17.81588056048429	26.834012780119586	30.896724053548326	24.4533826058478
4	12.581448096055267	18.929106888155385	39.4871529857136	29.002292030075743
5	13.034789231888775	38.328203022363134	35.47372604537758	13.163281700370504
6	28.685232737436138	39.27873529984182	18.93452352042779	13.101508442294246
7	25.418420315834528	33.30748392177433	23.40966457016688	17.864431192224266
8	23.43814853921649	37.71256886930697	21.47927118635836	17.370011405118177
9	24.58422996514243	17.566935842270652	21.889434051278204	35.95940014130872
10-11	23.287687004227557	27.59282823135901	29.34270725660209	19.776777507811342
12-13	24.277880545319857	24.844984768658772	29.527445991833503	21.34968869418787
14-15	21.36371054990378	26.429639652284532	27.14336887994304	25.063280917868646
16-17	20.925706631820624	29.799355735387984	27.770961355269485	21.50397627752191
18-19	20.90017693115073	28.74803102642185	29.758736728984438	20.593055313442985
20-21	21.582095724922812	27.73563984818675	29.720630016818482	20.961634410071955
22-23	21.301151805918593	27.936574306267936	29.544653633042415	21.217620254771056
24-25	21.20092088615723	27.997118037287994	29.673685080487555	21.128275996067224
26-27	21.15885269222801	28.092478470312223	29.63263646513017	21.116032372329602
28-29	20.9243071784723	28.0301153061761	29.8336149470679	21.2119625682837
30-31	21.32620630507582	27.803037559036557	29.549108749721736	21.321647386165886
32-33	21.264056484847433	27.868352760314373	29.749129742366172	21.11846101247202
34-35	21.22905467413317	28.12609643206071	29.702631953197777	20.94221694060834
36-37	21.11740212370428	27.978100128681326	29.605993663827206	21.29850408378719
38-39	21.06723238695551	27.912118645492868	29.78023112045718	21.240417847094445
40-41	21.26780307358064	27.90384747488661	29.697950602176785	21.130398849355966
42-43	21.374710369723623	27.86571445982706	29.570630861341403	21.18894430910791
44-45	21.25651437237798	28.110991657826595	29.35029365759278	21.282200312202644
46-47	21.46920481730602	27.931043801579857	29.030288635244467	21.56946274586966
48-49	21.41237437992399	27.99406262216142	29.005476583524505	21.58808641439009
50-51	21.24158530862983	28.09917193903707	29.105641150334154	21.553601601998945
52-53	21.352225582277313	28.132666358625542	29.166147668206243	21.348960390890902
54-55	21.339512840790203	28.109631869098255	28.983557912591856	21.567297377519683
56-57	21.599833847822556	28.364998177373955	28.634060737475387	21.401107237328105
58-59	21.250200187485184	28.681951577562643	28.96455103789526	21.103297197056918
60-61	21.522091057586064	28.866310358229324	28.55239153844728	21.059207045737327
62-63	21.282803498127063	29.13025876984912	28.4437141162953	21.143223615728516
64-65	21.243440724629064	29.016083697047456	28.527155646133238	21.213319932190235
66-67	21.3381498476165	28.93292523585758	28.43491095990196	21.294013956623957
68-69	21.335531003176794	28.712340822353326	28.59584464851454	21.356283525955337
70-71	21.379706367951076	28.408667631326306	28.706577343845918	21.505048656876696
72-73	21.502707398228495	28.276773399001858	28.75998849033451	21.460530712435137
74-75	21.463626586850946	28.32517434076149	28.6731103679025	21.538088704485066
76-77	21.488926739038497	28.301596763701447	28.606461217177724	21.60301528008233
78-79	21.428420675652827	28.23583158744703	28.679879038695816	21.65586869820433
80-81	21.456060532039583	28.283427220460837	28.67735856698639	21.583153680513192
82-83	21.525615955317203	28.299612536583062	28.66863604750536	21.50613546059437
84-85	21.63612201078923	28.24854256476262	28.62196887669975	21.4933665477484
86-87	21.48026937657155	28.302096286418227	28.641674799494233	21.575959537515992
88-89	21.543998342135115	28.27961546398749	28.61141134231655	21.564974851560844
90-91	21.589563009862314	28.34086080047817	28.565934075180664	21.503642114478858
92-93	21.60043067313471	28.315509217165314	28.575283012229058	21.508777097470922
94-95	21.47029076835566	28.407140387468072	28.569876978329	21.552691865847265
96-97	21.563250511475612	28.56010687069347	28.583033427944365	21.293609189886553
98-99	22.567595527980664	30.386043670276376	28.912244777235248	18.134116024507716
100	21.470637447085863	31.363200962305438	22.88788678659614	24.278274804012554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6182.0
1	6023.0
2	7176.0
3	9179.0
4	11878.5
5	16016.0
6	19778.0
7	26547.5
8	33413.5
9	36039.0
10	36222.0
11	36353.0
12	40714.5
13	52081.0
14	76719.0
15	106242.0
16	124516.0
17	129397.5
18	124405.0
19	114917.0
20	106441.0
21	103362.5
22	111427.0
23	133304.0
24	168164.0
25	216085.5
26	276281.0
27	343344.0
28	412082.5
29	489945.0
30	566166.5
31	636530.0
32	712366.0
33	781550.0
34	851534.0
35	919948.5
36	978967.0
37	1034274.5
38	1071833.5
39	1092916.5
40	1117980.5
41	1143449.0
42	1157680.0
43	1169253.5
44	1207654.0
45	1270321.0
46	1268591.0
47	1221802.5
48	1170966.0
49	1106425.0
50	1055219.5
51	998868.0
52	932451.0
53	867953.5
54	805115.5
55	733028.0
56	654047.0
57	574796.0
58	496498.5
59	419963.5
60	344667.5
61	274788.5
62	211695.0
63	158075.0
64	116756.0
65	85892.0
66	62178.5
67	46036.5
68	34679.0
69	25081.0
70	17802.0
71	12634.5
72	8819.5
73	5878.0
74	3858.0
75	2557.5
76	1642.5
77	980.0
78	568.0
79	350.5
80	229.5
81	145.5
82	106.0
83	71.0
84	42.5
85	28.5
86	21.5
87	18.5
88	10.0
89	4.0
90	2.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0033578378626932226
3	0.0
4	0.0
5	0.0028267334348791025
6	5.939983732131612E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.241469155150824E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.4017758818291718E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.2713175202864591E-5
48-49	4.2277887171556633E-4
50-51	0.0016403985409078903
52-53	0.004220452781676311
54-55	0.0059570178936866145
56-57	0.006655499194127935
58-59	0.008293842386570008
60-61	0.007839959928415135
62-63	0.005720317359746086
64-65	0.0034471849317055307
66-67	0.001200509978202923
68-69	4.7995039801516215E-4
70-71	2.3150500525795766E-4
72-73	5.830658275064915E-4
74-75	1.0170474234568074E-5
76-77	2.830616871001651E-4
78-79	2.793355100673656E-4
80-81	1.0230074586221145E-4
82-83	8.425818806853081E-6
84-85	1.4448912629018963E-4
86-87	1.3506456954696578E-4
88-89	2.3804967373885557E-5
90-91	4.371118542352609E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	307.0
20-21	977.0
22-23	1866.0
24-25	2748.0
26-27	6376.0
28-29	15910.0
30-31	43485.0
32-33	55989.0
34-35	79870.0
36-37	127054.0
38-39	151904.0
40-41	153629.0
42-43	178897.0
44-45	214311.0
46-47	216359.0
48-49	196964.0
50-51	178283.0
52-53	173934.0
54-55	185474.0
56-57	193897.0
58-59	193170.0
60-61	195991.0
62-63	196507.0
64-65	223062.0
66-67	251165.0
68-69	252835.0
70-71	292679.0
72-73	205834.0
74-75	201842.0
76-77	210097.0
78-79	215063.0
80-81	214487.0
82-83	206157.0
84-85	207059.0
86-87	215784.0
88-89	224290.0
90-91	228200.0
92-93	227563.0
94-95	278867.0
96-97	868979.0
98-99	2021449.0
100-101	1.9310293E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.45803094918003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.14565677331065	66.72672948484536
2	8.355434327425598	12.943909814551462
3	2.6375020299965937	6.12887141604004
4	1.1246276528981272	3.4844577417794738
5	0.6111739900658714	2.3670166918928053
6	0.3552490649925431	1.6510135842515803
7	0.22035551407333429	1.1947812960240363
8	0.1415052511077515	0.8768574495820568
9	0.09172643622311565	0.639445422224527
>10	0.31198970376206625	3.5966361819467254
>50	0.0038593180968067168	0.20342577182389682
>100	8.294505347582052E-4	0.1064781825714637
>500	5.0435860210424566E-5	0.025964192005671318
>1k	4.005165258571262E-5	0.0544127704609834
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0482324233173432E-5	0.0	1.7470540388622387E-5	0.0
3	0.0	1.7470540388622387E-5	0.0	2.4458756544071342E-5	0.0
4	0.0	8.036448578766298E-5	0.0	5.590572924359164E-5	3.4941080777244776E-6
5	0.0	9.434091809856089E-5	0.0	6.28939453990406E-5	3.4941080777244776E-6
6	8.036448578766298E-5	9.434091809856089E-5	0.0	7.337626963221403E-5	3.4941080777244776E-6
7	8.036448578766298E-5	9.783502617628537E-5	0.0	1.083173504094588E-4	3.4941080777244776E-6
8	8.385859386538746E-5	1.1181145848718328E-4	0.0	1.2928199887580566E-4	3.4941080777244776E-6
9	8.385859386538746E-5	1.7819951196394835E-4	0.0	1.6422307965305045E-4	3.4941080777244776E-6
10-11	8.735270194311194E-5	1.799465660028106E-4	0.0	1.8344067408053506E-4	3.4941080777244776E-6
12-13	9.434091809856089E-5	1.851877281193973E-4	0.0	2.0091121446915745E-4	3.4941080777244776E-6
14-15	9.434091809856089E-5	2.620581058293358E-4	0.0	2.393464033241267E-4	1.572348634976015E-5
16-17	1.991641604302952E-4	2.742874841013715E-4	0.0	2.568169437127491E-4	2.0964648466346864E-5
18-19	2.026582685080197E-4	2.917580244899939E-4	0.0	3.1796383507292747E-4	2.4458756544071342E-5
20-21	2.026582685080197E-4	3.197108891117897E-4	0.0	3.8085778047196805E-4	2.4458756544071342E-5
22-23	2.078994306246064E-4	3.598931320056212E-4	0.0	4.385105637544219E-4	3.6688134816107014E-5
24-25	2.1838175485777985E-4	4.053165370160394E-4	0.0	4.97910401075738E-4	5.2411621165867166E-5
26-27	2.2362291697436656E-4	4.2628118548238627E-4	0.0	5.520690762804675E-4	5.9399837321316115E-5
28-29	2.2362291697436656E-4	4.2977529356011076E-4	0.0	7.285215342055535E-4	5.9399837321316115E-5
30-31	2.3410524120754E-4	4.6821048241508E-4	0.0	0.0012473965837476383	6.28939453990406E-5
32-33	2.3759934928526446E-4	4.717045904928045E-4	0.0	0.0022746643585986348	7.16292155933518E-5
34-35	2.4633461947957567E-4	5.206221035809471E-4	0.0	0.0036967663462324973	8.211153982652522E-5
36-37	2.480816735184379E-4	5.870101570577122E-4	0.0	0.005929501407898438	8.385859386538746E-5
38-39	2.480816735184379E-4	6.167100757183703E-4	0.0	0.009344992053874115	8.385859386538746E-5
40-41	2.550698896738869E-4	6.568923186122018E-4	0.0	0.013057481886456373	8.385859386538746E-5
42-43	2.550698896738869E-4	6.743628590008241E-4	0.0	0.01645026082992684	8.385859386538746E-5
44-45	2.568169437127491E-4	7.617155609439361E-4	0.0	0.020536620226825616	9.259386405969865E-5
46-47	2.585639977516113E-4	8.368388846150123E-4	0.0	0.024851843702815346	9.958208021514762E-5
48-49	2.620581058293358E-4	8.578035330813593E-4	0.0	0.029402919474051478	1.0482324233173432E-4
50-51	2.8127570025682046E-4	9.591326673353691E-4	0.0	0.03385965432718905	1.0482324233173432E-4
52-53	2.900109704511316E-4	9.643738294519558E-4	0.0	0.037963484264476446	1.222937827203567E-4
54-55	2.935050785288561E-4	9.888325859960271E-4	0.0	0.0423887721449145	1.484995933032903E-4
56-57	3.0049329468430505E-4	0.001009797234462374	0.0	0.0467791189445753	1.5024664734215252E-4
58-59	3.0049329468430505E-4	0.0010272677748509964	0.0	0.051462970822764964	1.5024664734215252E-4
60-61	3.1796383507292747E-4	0.0010587147475505168	0.0	0.05611712278229397	1.5374075541987702E-4
62-63	3.4941080777244776E-4	0.0010866676121723126	0.0	0.06047252850117753	1.6771718773077492E-4
64-65	3.5290491585017225E-4	0.001207214340853807	0.0	0.0649013104896933	1.6771718773077492E-4
66-67	3.6338724008334563E-4	0.0012299260433590161	0.0	0.06936852766706406	1.7121129580849938E-4
68-69	3.668813481610701E-4	0.0012631200700973988	0.0	0.07375887446672486	1.7470540388622388E-4
70-71	3.7212251027765684E-4	0.0012840847185637454	0.0	0.07782426921515728	1.834406740805351E-4
72-73	4.053165370160394E-4	0.001294567042796919	0.0	0.08058286754252077	2.026582685080197E-4
74-75	4.053165370160394E-4	0.0013260140154964391	0.0	0.08291343763036299	2.026582685080197E-4
76-77	4.070635910549016E-4	0.0013784256366623064	3.4941080777244776E-6	0.0852125607455057	2.0440532254688195E-4
78-79	4.2278707740466177E-4	0.001397643231089791	3.4941080777244776E-6	0.08683207983953098	2.0615237658574417E-4
80-81	4.2628118548238627E-4	0.0014168608255172757	3.4941080777244776E-6	0.08747324867179343	2.0964648466346867E-4
82-83	4.31522347598973E-4	0.0014203549335950001	3.4941080777244776E-6	0.08801658247787958	2.166347008189176E-4
84-85	4.367635097155597E-4	0.0014325843118670359	3.4941080777244776E-6	0.08834328158314683	2.1838175485777982E-4
86-87	4.367635097155597E-4	0.001450054852255658	3.4941080777244776E-6	0.0885109987708776	2.2362291697436656E-4
88	4.472458339487331E-4	0.0015269252299655966	3.4941080777244776E-6	0.08851623993299419	2.2362291697436656E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	33320	0.0	44.602417	1
GTATCAA	62360	0.0	36.480385	1
CTTATAC	26170	0.0	35.739803	1
ATCAACG	75720	0.0	31.181276	3
TCAACGC	76330	0.0	30.937737	4
CAACGCA	77705	0.0	30.3681	5
TATCAAC	80200	0.0	29.692995	2
AACGCAG	81250	0.0	29.148445	6
TATACAC	41700	0.0	26.294415	3
CGCAGAG	94605	0.0	24.983522	8
ACGCAGA	94740	0.0	24.961569	7
TTATACA	40985	0.0	24.461376	2
GCAGAGT	111580	0.0	21.132488	9
ACATCTC	47090	0.0	20.657284	8
ACACATC	48555	0.0	19.750002	6
TACACAT	56130	0.0	19.250488	5
AGAGTAC	102115	0.0	18.94445	10-11
GAGTACT	69855	0.0	18.484795	12-13
ATCTCCG	31890	0.0	18.042515	7
TATGCCG	30795	0.0	17.965027	42-43
>>END_MODULE