FastQCFastQC Report
Fri 27 May 2016
SRR2139062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16390813
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA264000.041.1203541
GTATCAA455550.033.4837761
ATCAACG529750.027.9511433
TCAACGC531700.027.8887394
CAACGCA538800.027.5372335
AACGCAG556700.026.6824516
TATCAAC575750.026.159942
ACGCAGA640550.023.1369367
CGCAGAG653350.022.6771268
GCAGAGT738250.019.8496919
AGAGTAC653250.019.14205710-11
GTACATG459650.018.8104631
GAGTACT441900.018.8100212-13
TACATGG461450.018.2656562
CAGAGTA704600.017.69859510-11
AGTACTT490800.017.55287412-13
ACATGGG464150.017.3657783
GTACTTT497250.016.71656614-15
CTTATAC96750.015.5190331
CATGGGG327000.015.1417224
Produced by FastQC (version 0.11.5)