FastQCFastQC Report
Fri 27 May 2016
SRR2139061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12454102
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165100.049.3126451
GTATCAA308100.036.5713161
ATCAACG371700.029.6406563
TCAACGC372650.029.5994194
CAACGCA382050.028.8492935
TATCAAC391600.028.5010282
AACGCAG393500.028.0424586
ACGCAGA452850.024.3775447
CGCAGAG457850.024.1113268
GCAGAGT534700.020.5741449
GTGGTAT86450.019.8810581
CTTATAC85450.019.6146221
GTACATG349550.019.3383481
GAGTACT326600.019.17176812-13
TACATGG351950.019.0489482
AGAGTAC485200.018.93820210-11
TGGTATC85450.018.6663322
ACATGGG356250.018.3115223
CAGAGTA521200.018.0800710-11
AGTACTT362150.017.3015712-13
Produced by FastQC (version 0.11.5)