FastQCFastQC Report
Fri 27 May 2016
SRR2139060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10668104
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153300.046.802331
GTATCAA267200.034.6649551
CTTATAC100150.034.288751
ATCAACG320800.028.1499393
TCAACGC323900.027.8805224
CAACGCA329200.027.4447235
AACGCAG342350.026.5399766
TATCAAC344200.026.5264342
TATACAC151000.026.4607333
TTATACA152950.023.9282672
ACGCAGA389200.023.2145397
CGCAGAG395150.022.8542048
ACATCTC168600.020.5664378
ACACATC172600.020.2866826
GCAGAGT457150.019.6241979
GAGTACT283850.019.29178212-13
GTACATG298500.019.254631
TACACAT208250.019.1660485
TACATGG292450.018.6914332
AGAGTAC415750.018.20225510-11
Produced by FastQC (version 0.11.5)