##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139060_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10668104
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47399669144583	34.0	31.0	34.0	31.0	34.0
2	32.61436390196421	34.0	31.0	34.0	31.0	34.0
3	32.673899035854916	34.0	31.0	34.0	31.0	34.0
4	36.195448975750516	37.0	35.0	37.0	35.0	37.0
5	36.11276014931988	37.0	35.0	37.0	35.0	37.0
6	36.14779833417447	37.0	36.0	37.0	35.0	37.0
7	36.099372484557705	37.0	36.0	37.0	35.0	37.0
8	36.07408214243131	37.0	36.0	37.0	35.0	37.0
9	37.78913432040033	39.0	38.0	39.0	35.0	39.0
10-11	37.71709110634842	39.0	37.5	39.0	35.0	39.0
12-13	37.661228087015274	39.0	37.0	39.0	35.0	39.0
14-15	39.00334234649381	40.0	38.0	41.0	35.5	41.0
16-17	38.972060030535886	40.0	38.0	41.0	35.0	41.0
18-19	38.97012505689858	40.0	38.0	41.0	35.0	41.0
20-21	38.94556152067254	40.0	38.0	41.0	35.0	41.0
22-23	38.843062916865144	40.0	38.0	41.0	35.0	41.0
24-25	38.71419686676184	40.0	38.0	41.0	34.0	41.0
26-27	38.640686602646426	40.0	38.0	41.0	34.5	41.0
28-29	38.58584857797503	40.0	38.0	41.0	34.0	41.0
30-31	38.50970710772745	40.0	38.0	41.0	34.0	41.0
32-33	38.401521133737106	40.0	38.0	41.0	34.0	41.0
34-35	38.289973798521615	40.0	38.0	41.0	34.0	41.0
36-37	38.42262283992318	40.0	38.0	41.0	34.0	41.0
38-39	38.47822141035111	40.0	38.0	41.0	34.0	41.0
40-41	38.419705694122854	40.0	38.0	41.0	34.0	41.0
42-43	38.38778383645829	40.0	38.0	41.0	34.0	41.0
44-45	38.35968183881907	40.0	38.0	41.0	34.0	41.0
46-47	38.26764923378959	40.0	38.0	41.0	33.0	41.0
48-49	38.19796207058164	40.0	38.0	41.0	33.0	41.0
50-51	38.123598991515976	40.0	37.5	41.0	33.0	41.0
52-53	38.05314245226441	40.0	37.0	41.0	33.0	41.0
54-55	37.93792511034698	40.0	37.0	41.0	33.0	41.0
56-57	37.78164990812422	40.0	37.0	41.0	33.0	41.0
58-59	37.64964597762058	39.5	36.0	41.0	33.0	41.0
60-61	37.50213133408053	39.0	36.0	41.0	32.5	41.0
62-63	37.28973352670149	39.0	35.5	41.0	32.0	41.0
64-65	36.974310954698076	38.5	35.0	40.0	31.5	41.0
66-67	36.68364864071975	38.0	35.0	40.0	31.0	41.0
68-69	36.410358698813454	37.5	35.0	40.0	31.0	41.0
70-71	36.10425184951912	37.0	35.0	39.5	31.0	41.0
72-73	35.79504601557801	36.5	34.5	39.0	31.0	41.0
74-75	35.469646185213094	36.0	34.0	39.0	30.0	40.0
76-77	34.49370655076335	35.0	33.5	37.0	29.5	39.0
78-79	34.80878730716914	35.0	34.0	37.0	30.0	39.0
80-81	34.64716412810134	35.0	34.0	37.0	30.0	39.0
82-83	34.403282032174	35.0	34.0	36.5	30.0	39.0
84-85	34.1391303430693	35.0	34.0	36.0	30.0	37.5
86-87	33.90579359691857	35.0	34.0	36.0	30.0	37.0
88-89	33.69469191379035	35.0	33.5	35.0	30.0	37.0
90-91	33.522336240197134	35.0	33.0	35.0	29.0	36.0
92-93	33.39020490928428	35.0	33.0	35.0	29.0	36.0
94-95	33.32851255623497	35.0	33.0	35.0	29.0	36.0
96-97	33.34696386708171	35.0	33.0	35.0	29.5	35.5
98-99	33.391687243967	35.0	33.0	35.0	30.0	35.0
100-101	31.916281644567917	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	11.0
20	61.0
21	342.0
22	1653.0
23	5584.0
24	14158.0
25	28699.0
26	50226.0
27	78204.0
28	111018.0
29	147851.0
30	188049.0
31	234360.0
32	292966.0
33	376113.0
34	511359.0
35	720716.0
36	1166737.0
37	2208888.0
38	2994230.0
39	1464653.0
40	72226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.12245362437412	21.798222064576798	15.8308542923841	24.248470018664985
2	13.475917067125359	21.673392597531933	41.98033769229659	22.87035264304612
3	16.82151767549323	26.72087748675866	31.517381157888973	24.940223679859137
4	11.788111551968372	19.08169436668409	38.97464816615961	30.155545915187925
5	12.622392882559074	38.19396586309995	36.10663150640451	13.077009747936467
6	29.218500307083623	38.83518570872575	18.738418748073695	13.207895236116935
7	25.730289093544645	33.16451545654223	23.215446718554674	17.88974873135845
8	23.835519413758995	37.58843183381039	21.19460965134948	17.381439101081128
9	24.860603158724363	17.185968565735767	21.597492862836734	36.35593541270314
10-11	23.56708523374862	27.347452859999045	29.189591400050784	19.895870506201547
12-13	24.482606327111768	24.76204233507077	29.351335848944828	21.40401548887264
14-15	21.536122358035197	26.323853109016014	27.0081688382807	25.131855694668086
16-17	21.050221295180474	29.59738675213515	27.693449557672107	21.658942395012275
18-19	21.04142404311019	28.546487735777603	29.638382790418994	20.773705430693216
20-21	21.79113496313855	27.55355375257676	29.63247101664166	21.022840267643033
22-23	21.438731882176185	27.75511484051403	29.482011597582236	21.324141679727557
24-25	21.379711487717795	27.98578046274217	29.532261811849253	21.10224623769078
26-27	21.431097829223667	28.13618861802319	29.382447750539303	21.05026580221384
28-29	21.111417900988442	28.195347889362026	29.470821049443625	21.222413160205907
30-31	21.707930622220424	27.89405972298773	29.42071154671515	20.977298108076695
32-33	21.101807836426435	28.19002591637765	29.424629211411368	21.28353703578455
34-35	21.306687368023177	28.19855443640765	29.554035156487952	20.94072303908122
36-37	21.260304273679857	28.226201703485316	29.351553656060087	21.161940366774736
38-39	21.18407668919085	27.958621704516943	29.520023540978386	21.337278065313818
40-41	21.436022900441042	27.873293310660642	29.420805200388457	21.269878588509858
42-43	21.481031274096203	27.934258305521038	29.270818638070413	21.313891782312346
44-45	21.420125919831168	28.16068944808108	28.997070796736562	21.42211383535119
46-47	21.648868685744375	28.02071266816975	28.585172832002993	21.74524581408288
48-49	21.59795393645008	28.104450727641805	28.573021572656792	21.724573763251325
50-51	21.421345659150056	28.252595296958226	28.560860923898318	21.765198119993396
52-53	21.525045700720575	28.28865140871673	28.594921056519002	21.59138183404369
54-55	21.57875702644875	28.298027261911667	28.366725965154988	21.756489746484593
56-57	21.7579342299439	28.44659083092272	28.135818818644697	21.659656120488684
58-59	21.45290875289462	28.725036899442486	28.439745061221235	21.38230928644166
60-61	21.767452356790766	28.780359034305807	28.071355937261373	21.380832671642054
62-63	21.550491189366756	28.902186765361705	28.020218438544298	21.527103606727245
64-65	21.509340240373177	28.825685948890634	28.07812874490267	21.586845065833522
66-67	21.64165785093896	28.706345302508605	27.99114339426854	21.66085345228389
68-69	21.64962887526588	28.527173387689686	28.09659116775418	21.726606569290254
70-71	21.624864387666932	28.32053808152982	28.188594966978137	21.86600256382512
72-73	21.72097984558372	28.30006053107435	28.211659234143642	21.767300389198283
74-75	21.609215435291134	28.396678410533728	28.168970323222105	21.82513583095304
76-77	21.65499760989069	28.369794489190536	28.09096637428618	21.884241526632593
78-79	21.60800296958498	28.337613322806977	28.134340345152893	21.920043362455154
80-81	21.621288743456862	28.352170491795675	28.16229123864827	21.86424952609919
82-83	21.64625965803918	28.327268873464973	28.285803650851566	21.74066781764428
84-85	21.81857859370288	28.297901822141164	28.17043000177713	21.713089582378828
86-87	21.663938071372176	28.350391256072704	28.184028184652504	21.801642487902615
88-89	21.742093606695917	28.211218457824156	28.193809294073308	21.85287864140662
90-91	21.77592515720986	28.2256196982703	28.226180903255106	21.772274241264736
92-93	21.768894065558754	28.13047738494548	28.308089969163795	21.79253858033197
94-95	21.580372435098244	28.189570673603086	28.377291646117442	21.85276524518123
96-97	21.55084604830998	28.263151945703243	28.38328378996383	21.80271821602295
98-99	21.772723967368762	28.817651840568264	28.42471796089836	20.98490623116461
100-101	21.947426393635652	31.666188838545352	25.80754170470253	20.578843063116466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2001.0
1	1851.0
2	2634.5
3	3827.0
4	5321.0
5	8297.0
6	10860.0
7	13424.5
8	15558.5
9	15818.0
10	14428.0
11	12960.0
12	14288.5
13	19993.5
14	34068.0
15	52703.0
16	66772.5
17	73825.5
18	74055.5
19	69615.5
20	63990.0
21	58855.5
22	56275.0
23	60145.0
24	70100.0
25	85268.0
26	104274.0
27	125168.0
28	149159.5
29	175636.0
30	202567.0
31	229437.5
32	256621.0
33	284704.0
34	310643.0
35	332514.0
36	355743.5
37	377836.5
38	394079.0
39	404631.0
40	413999.0
41	422862.0
42	427054.0
43	430944.0
44	436354.0
45	445176.5
46	455411.5
47	458459.5
48	445967.0
49	429006.5
50	414702.0
51	393083.0
52	366849.5
53	340630.5
54	315202.0
55	288100.5
56	261303.0
57	232972.0
58	201907.0
59	171847.0
60	141599.5
61	113917.0
62	89572.5
63	67404.0
64	49565.5
65	37353.0
66	28173.5
67	21100.0
68	15888.5
69	11074.0
70	7631.5
71	5566.0
72	3941.0
73	2561.5
74	1630.5
75	1061.5
76	713.0
77	472.5
78	321.0
79	212.0
80	148.5
81	103.5
82	76.0
83	52.0
84	35.0
85	24.5
86	18.0
87	14.5
88	9.0
89	6.5
90	6.5
91	5.5
92	4.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.022665695797491288
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	3.843232124471227E-4
12-13	0.001757575666678915
14-15	1.8747473777908427E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008378349968601113
26-27	0.026894123221330613
28-29	0.32799829057366164
30-31	0.3130882209084633
32-33	0.31547969160985606
34-35	0.1692751687510824
36-37	0.031218017532152424
38-39	0.029922724337962704
40-41	0.012108974630834593
42-43	0.009516793275443533
44-45	0.0036200190267810274
46-47	0.002233954804141949
48-49	0.0020165782600744227
50-51	0.0020585345037429266
52-53	5.450595974724779E-4
54-55	1.068012457297302E-4
56-57	4.360145561151247E-4
58-59	9.784533696303294E-4
60-61	0.003376066354859589
62-63	0.0033929840688052843
64-65	0.00321438252271457
66-67	0.006904764805168666
68-69	0.01060859133443813
70-71	0.014498472923000109
72-73	0.013891451077502613
74-75	0.010690933756716033
76-77	0.005056345049072084
78-79	0.002001321675128033
80-81	0.0020874974558624756
82-83	2.991117436900912E-4
84-85	3.5590072125653834E-5
86-87	0.0
88-89	6.62796462320842E-4
90-91	0.0024544406782963987
92-93	0.014516563975649748
94-95	4.139013809278319E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	236.0
20-21	807.0
22-23	1689.0
24-25	3921.0
26-27	5679.0
28-29	9270.0
30-31	20665.0
32-33	26753.0
34-35	39715.0
36-37	54382.0
38-39	66007.0
40-41	69456.0
42-43	83113.0
44-45	99767.0
46-47	97770.0
48-49	86320.0
50-51	77137.0
52-53	74466.0
54-55	81149.0
56-57	89285.0
58-59	90196.0
60-61	92431.0
62-63	93924.0
64-65	99831.0
66-67	107089.0
68-69	97404.0
70-71	90305.0
72-73	87310.0
74-75	89181.0
76-77	89894.0
78-79	95537.0
80-81	98181.0
82-83	95700.0
84-85	98790.0
86-87	104528.0
88-89	112527.0
90-91	122909.0
92-93	127366.0
94-95	145396.0
96-97	224128.0
98-99	928323.0
100-101	6589567.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.67272841912458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.35696747077552	73.0471157564885
2	7.723735398682371	12.77084557992893
3	2.074366333689861	5.144805736407511
4	0.8371183823715649	2.7682744272184507
5	0.4062703734672335	1.6793740125196455
6	0.1964873116364013	0.974648529163206
7	0.12278731686715788	0.7105813750470077
8	0.08241911598522042	0.5451050553912364
9	0.046956500189384354	0.34938197889025896
>10	0.15095936812981692	1.8413785696792018
>50	0.0015276920460821272	0.08634015207129096
>100	3.657345139776981E-4	0.057869387138083546
>500	3.900164541321719E-5	0.024279440056722074
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.686868444477107E-5	0.0	0.0	0.0
3	0.0	4.686868444477107E-5	0.0	9.373736888954214E-6	0.0
4	0.0	1.2185857955640478E-4	0.0	1.8747473777908427E-5	0.0
5	0.0	1.31232316445359E-4	0.0	1.8747473777908427E-5	0.0
6	0.0	1.31232316445359E-4	0.0	2.812121066686264E-5	9.373736888954214E-6
7	0.0	1.31232316445359E-4	0.0	3.7494947555816855E-5	9.373736888954214E-6
8	0.0	1.4997979022326742E-4	0.0	3.7494947555816855E-5	9.373736888954214E-6
9	0.0	2.4371715911280955E-4	0.0	6.56161582226795E-5	9.373736888954214E-6
10-11	0.0	2.4371715911280955E-4	0.0	7.967676355611082E-5	9.373736888954214E-6
12-13	0.0	2.4371715911280955E-4	0.0	9.842423733401925E-5	9.373736888954214E-6
14-15	0.0	3.2808079111339746E-4	0.0	1.0311110577849635E-4	1.8747473777908427E-5
16-17	0.0	3.2808079111339746E-4	0.0	1.4997979022326742E-4	1.8747473777908427E-5
18-19	0.0	3.421413964468288E-4	0.0	2.155959484459469E-4	1.8747473777908427E-5
20-21	0.0	3.7026260711369146E-4	0.0	2.858989751131035E-4	1.8747473777908427E-5
22-23	0.0	4.5931310755875643E-4	0.0	3.233939226689204E-4	2.812121066686264E-5
24-25	0.0	5.061817920035275E-4	0.0	3.8901008089159986E-4	3.7494947555816855E-5
26-27	0.0	5.155555288924818E-4	0.0	5.530504764482986E-4	4.686868444477107E-5
28-29	0.0	5.29616134225913E-4	0.0	8.717575306727418E-4	4.686868444477107E-5
30-31	0.0	5.717979502262069E-4	0.0	0.0018185049564571174	4.686868444477107E-5
32-33	0.0	5.811716871151612E-4	0.0	0.003707312939581391	4.686868444477107E-5
34-35	0.0	6.655353191157491E-4	0.0	0.005957009792930403	5.624242133372528E-5
36-37	0.0	7.545858195608142E-4	0.0	0.009687757074734179	5.624242133372528E-5
38-39	0.0	7.686464248942455E-4	0.0	0.01555102949877504	5.624242133372528E-5
40-41	0.0	8.436363200058793E-4	0.0	0.021897049372597042	5.624242133372528E-5
42-43	0.0	8.623837937837876E-4	0.0	0.028271190457085905	5.624242133372528E-5
44-45	0.0	9.373736888954214E-4	0.0	0.035807674915805096	5.624242133372528E-5
46-47	0.0	9.983029786736239E-4	0.0	0.04364880582341529	6.092928977820239E-5
48-49	0.0	0.0010029898471181008	0.0	0.051424320572802815	6.56161582226795E-5
50-51	0.0	0.0011295352951189827	0.0	0.05940137066530285	6.56161582226795E-5
52-53	0.0	0.0011529696373413683	0.0	0.06721906723069067	8.436363200058792E-5
54-55	0.0	0.0011764039795637538	0.0	0.07537421832408082	1.0311110577849635E-4
56-57	0.0	0.0012092120586750935	0.0	0.08168743011879148	1.0311110577849635E-4
58-59	0.0	0.001232646400897479	0.0	0.08706795509305121	1.0311110577849635E-4
60-61	0.0	0.0012654544800088188	0.0	0.09222351038197603	1.0311110577849635E-4
62-63	0.0	0.0012888888222312043	0.0	0.0971259747748991	1.2185857955640478E-4
64-65	0.0	0.0014154342702320862	0.0	0.10251587348604776	1.2185857955640478E-4
66-67	0.0	0.0014341817440099948	0.0	0.10778860048608449	1.31232316445359E-4
68-69	0.0	0.0014576160862323803	0.0	0.11321130727634451	1.406060533343132E-4
70-71	0.0	0.0014763635600102886	0.0	0.11837154943371381	1.4997979022326742E-4
72-73	0.0	0.001495111033788197	0.0	0.1226506603235214	1.6872726400117585E-4
74-75	0.0	0.0015701009288998308	0.0	0.12622205407821296	1.6872726400117585E-4
76-77	0.0	0.0016544645609004186	0.0	0.12955441754223618	1.6872726400117585E-4
78-79	0.0	0.0016778989031228041	0.0	0.1323196699244777	1.6872726400117585E-4
80-81	9.373736888954214E-6	0.0016778989031228041	0.0	0.1334632658249301	1.7341413244565296E-4
82-83	9.373736888954214E-6	0.0016778989031228041	0.0	0.13402100316982285	1.7810100089013005E-4
84-85	9.373736888954214E-6	0.0016919595084562357	0.0	0.13439126577693655	1.7810100089013005E-4
86-87	9.373736888954214E-6	0.001752888798234438	0.0	0.13449437688271504	1.7810100089013005E-4
88-89	9.373736888954214E-6	0.0018700605093463656	0.0	0.13455999304093774	1.8747473777908427E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15330	0.0	46.80233	1
GTATCAA	26720	0.0	34.664955	1
CTTATAC	10015	0.0	34.28875	1
ATCAACG	32080	0.0	28.149939	3
TCAACGC	32390	0.0	27.880522	4
CAACGCA	32920	0.0	27.444723	5
AACGCAG	34235	0.0	26.539976	6
TATCAAC	34420	0.0	26.526434	2
TATACAC	15100	0.0	26.460733	3
TTATACA	15295	0.0	23.928267	2
ACGCAGA	38920	0.0	23.214539	7
CGCAGAG	39515	0.0	22.854204	8
ACATCTC	16860	0.0	20.566437	8
ACACATC	17260	0.0	20.286682	6
GCAGAGT	45715	0.0	19.624197	9
GAGTACT	28385	0.0	19.291782	12-13
GTACATG	29850	0.0	19.25463	1
TACACAT	20825	0.0	19.166048	5
TACATGG	29245	0.0	18.691433	2
AGAGTAC	41575	0.0	18.202255	10-11
>>END_MODULE