FastQCFastQC Report
Fri 27 May 2016
SRR2139059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10670533
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152050.045.6481
GTATCAA275000.036.2591251
CTTATAC88300.032.9243621
ATCAACG337200.029.1094843
TCAACGC338200.029.0359764
CAACGCA342850.028.6794825
AACGCAG352400.027.9507666
TATCAAC357500.027.6657432
ACGCAGA405150.024.280837
CGCAGAG409600.023.9962888
TATACAC138550.023.9220373
TTATACA138450.022.379822
GCAGAGT470550.020.6893929
GAGTACT293900.019.49012612-13
AGAGTAC425550.019.42215310-11
GTACATG298800.019.1036971
ACACATC154950.018.9222896
TACATGG297150.018.8950962
ACATCTC154400.018.8526178
ACATGGG300800.018.293723
Produced by FastQC (version 0.11.5)