FastQCFastQC Report
Fri 27 May 2016
SRR2139058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9988739
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC312450.023.325714
ATCAACG313550.023.2303983
GGTATCA234400.023.181361
GTATCAA328500.023.067261
CAACGCA316000.023.0370275
CTTATAC83300.022.8434091
AACGCAG324150.022.6274686
ACGCAGA372600.019.617397
CGCAGAG375350.019.4623998
GTACATG237200.019.2888361
TATACAC129800.018.9876863
TACATGG237450.018.8589362
ACATGGG232600.018.7381673
TATCAAC399850.018.6338332
AGAGTAC322150.018.31305310-11
GAGTACT226450.018.28596512-13
GCAGAGT428050.016.9971229
TATGCCG62850.016.97460242-43
CAGAGTA354150.016.93886210-11
AGTACTT247850.016.7582812-13
Produced by FastQC (version 0.11.5)