##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139058_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9988739
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45962047862098	34.0	31.0	34.0	31.0	34.0
2	32.59528465004441	34.0	31.0	34.0	31.0	34.0
3	32.65682785384622	34.0	31.0	34.0	31.0	34.0
4	36.182946916522695	37.0	35.0	37.0	35.0	37.0
5	36.10009091237643	37.0	35.0	37.0	35.0	37.0
6	36.134574444281704	37.0	36.0	37.0	35.0	37.0
7	36.08587149989603	37.0	36.0	37.0	35.0	37.0
8	36.05908403453129	37.0	35.0	37.0	35.0	37.0
9	37.77027881096903	39.0	38.0	39.0	35.0	39.0
10-11	37.70036082632653	39.0	37.5	39.0	35.0	39.0
12-13	37.6477972845221	39.0	37.0	39.0	35.0	39.0
14-15	38.990081981319165	40.0	38.0	41.0	35.0	41.0
16-17	38.96286448169283	40.0	38.0	41.0	35.0	41.0
18-19	38.9631797367015	40.0	38.0	41.0	35.0	41.0
20-21	38.935472046648826	40.0	38.0	41.0	35.0	41.0
22-23	38.83439945592528	40.0	38.0	41.0	35.0	41.0
24-25	38.710673414395615	40.0	38.0	41.0	34.0	41.0
26-27	38.641663912682965	40.0	38.0	41.0	34.5	41.0
28-29	38.583587773176774	40.0	38.0	41.0	34.0	41.0
30-31	38.5201071385667	40.0	38.0	41.0	34.0	41.0
32-33	38.4216433426197	40.0	38.0	41.0	34.0	41.0
34-35	38.309255778419704	40.0	38.0	41.0	34.0	41.0
36-37	38.449196470141615	40.0	38.0	41.0	34.0	41.0
38-39	38.51411251700212	40.0	38.0	41.0	34.0	41.0
40-41	38.45633436432314	40.0	38.0	41.0	34.0	41.0
42-43	38.427448064328665	40.0	38.0	41.0	34.0	41.0
44-45	38.40415693552755	40.0	38.0	41.0	34.0	41.0
46-47	38.31702734363721	40.0	38.0	41.0	33.0	41.0
48-49	38.24733122795331	40.0	38.0	41.0	33.0	41.0
50-51	38.18487509378308	40.0	38.0	41.0	33.0	41.0
52-53	38.11678480766661	40.0	37.0	41.0	33.0	41.0
54-55	38.00549591073022	40.0	37.0	41.0	33.0	41.0
56-57	37.85908865992744	40.0	37.0	41.0	33.0	41.0
58-59	37.740582778955144	40.0	36.5	41.0	33.0	41.0
60-61	37.602848211523735	39.0	36.0	41.0	33.0	41.0
62-63	37.39876227771211	39.0	36.0	41.0	32.0	41.0
64-65	37.087442998442	39.0	35.0	40.5	31.5	41.0
66-67	36.799944406302316	38.0	35.0	40.0	31.0	41.0
68-69	36.53021744670909	38.0	35.0	40.0	31.0	41.0
70-71	36.228918667583855	37.0	35.0	40.0	31.0	41.0
72-73	35.92002549774672	37.0	35.0	39.0	31.0	41.0
74-75	35.59350842490781	36.0	34.0	39.0	30.5	40.5
76-77	34.60285125350289	35.0	33.5	38.0	29.5	39.0
78-79	34.90905223740996	35.0	34.0	37.0	30.0	39.0
80-81	34.742754646407164	35.0	34.0	37.0	30.0	39.0
82-83	34.49064549208545	35.0	34.0	37.0	30.0	39.0
84-85	34.215352540648226	35.0	34.0	36.0	30.0	37.5
86-87	33.9737262215738	35.0	34.0	36.0	30.0	37.0
88-89	33.75624275297472	35.0	34.0	35.5	30.0	37.0
90-91	33.57856535385166	35.0	33.0	35.0	30.0	36.0
92-93	33.441206922086266	35.0	33.0	35.0	29.0	36.0
94-95	33.374895879692005	35.0	33.0	35.0	29.0	36.0
96-97	33.387179243956346	35.0	33.0	35.0	30.0	36.0
98-99	33.42707869333208	35.0	33.0	35.0	30.0	35.0
100-101	31.98024695270988	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	1.0
16	0.0
17	0.0
18	2.0
19	9.0
20	38.0
21	345.0
22	1432.0
23	4966.0
24	12501.0
25	25789.0
26	45117.0
27	71580.0
28	102074.0
29	136335.0
30	174842.0
31	217383.0
32	271768.0
33	347723.0
34	468821.0
35	656231.0
36	1050994.0
37	1986097.0
38	2834140.0
39	1503107.0
40	77442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.96990791330117	22.12247211584966	15.652296050582562	24.255323920266612
2	13.287643565335976	22.060223215765394	42.01948614331715	22.632647075581477
3	16.69059527934407	27.01469124380965	31.847903924609504	24.446809552236772
4	11.606620215024138	19.309013880530866	39.73638714556462	29.347978758880377
5	12.344441075094664	38.46746821595799	36.458065427477884	12.730025281469462
6	28.645137289101257	39.41744798817949	19.160766939650742	12.776647783068512
7	25.133502837545358	33.4700306014603	23.859007628490442	17.537458932503895
8	23.439064730793348	37.87569181655462	21.657408407607807	17.027835045044224
9	24.706532025714157	17.373564370838	22.089895431245125	35.83000817220272
10-11	23.191721208691277	27.560095505878667	29.734246317928413	19.513936967501643
12-13	24.16732675566989	24.940266617223397	29.916943917396637	20.975462709710076
14-15	21.251105432825348	26.630235291567715	27.425130781081425	24.69352849452551
16-17	20.729883922284888	29.898183344263973	28.134307043161304	21.237625690289835
18-19	20.716894294665224	29.064289296176426	29.810214282303306	20.40860212685505
20-21	21.49305406233083	28.12192216658846	29.642282069806967	20.74274170127374
22-23	21.16824125559541	28.06007211923441	29.602714221599335	21.168972403570844
24-25	21.161987445532198	28.298992312794624	29.621094840697825	20.917925400975356
26-27	21.267358386876094	28.40932960999722	29.427658895434128	20.89565310769256
28-29	20.970421806234185	28.4986612780624	29.587196579635272	20.943720336068143
30-31	21.405325197824375	28.339282970098985	29.502422262138396	20.752969569938255
32-33	20.93291760172696	28.501349774335665	29.466919305275134	21.098813318662234
34-35	21.110440397095072	28.51065676559243	29.4526912931002	20.926211544212293
36-37	21.08829407308905	28.475879924484765	29.41422420069478	21.021601801731414
38-39	21.094201607803527	28.299455065765194	29.52062936152816	21.08571396490311
40-41	21.296881618318878	28.258706416544033	29.39496958318574	21.049442381951348
42-43	21.216288716228814	28.379990694178957	29.256353530946356	21.147367058645873
44-45	21.227567245598184	28.616907135028523	28.95041834170152	21.205107277671768
46-47	21.357034763689416	28.474298914841782	28.675041424271345	21.493624897197453
48-49	21.386693845880817	28.478239194270376	28.636602927745063	21.498464032103744
50-51	21.291126921513346	28.6376349133376	28.61730571794222	21.45393244720683
52-53	21.374618141459766	28.590456383738534	28.66982162650851	21.365103848293188
54-55	21.40913679199687	28.590510759329085	28.523999049863946	21.4763533988101
56-57	21.488088822828143	28.74185457410803	28.38077576346183	21.389280839602
58-59	21.30912997617648	28.906231940960943	28.526362360677865	21.258275722184713
60-61	21.529063900394487	28.906868022764293	28.30127621432713	21.262791862514085
62-63	21.395810422980983	28.97199591580113	28.299778333106783	21.332415328111097
64-65	21.379516515279924	28.92049584801678	28.358830072659778	21.341157564043513
66-67	21.47712373127016	28.8512831265139	28.25471039229529	21.41688274992065
68-69	21.448896757403215	28.768485706925212	28.32851436453076	21.454103171140815
70-71	21.440931380283526	28.604243549178747	28.366099791576673	21.588725278961057
72-73	21.51997692593609	28.579205863628605	28.360726775340094	21.540090435095212
74-75	21.43234303350215	28.651573115812667	28.304546423147386	21.611537427537797
76-77	21.465651539830972	28.636575672975095	28.27421663105994	21.62355615613399
78-79	21.46523033001245	28.605639583110264	28.27679722398122	21.652332862896063
80-81	21.421177075873928	28.60450670327462	28.353125653999665	21.621190566851787
82-83	21.480950772236405	28.573173367562738	28.404124534401287	21.54175132579957
84-85	21.610172085731914	28.540678837208926	28.33378928560496	21.5153597914542
86-87	21.475295162581155	28.506319404844742	28.374030472026394	21.64435496054771
88-89	21.542795688559575	28.466135500370353	28.355770104179108	21.63529870689096
90-91	21.556137340538527	28.507436533499504	28.391291632788267	21.5451344931737
92-93	21.548762817689195	28.388505397024254	28.47125956392407	21.591472221362476
94-95	21.429857241222557	28.440785238286708	28.517379742074823	21.611977778415913
96-97	21.38587618183621	28.449788315757147	28.576101344772418	21.58823415763423
98-99	21.60473489237134	29.053217773664226	28.605765082931043	20.736282251033394
100-101	21.77178658206663	31.869885433715222	26.006647465863686	20.351680518354467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1386.0
1	1331.0
2	2104.0
3	3121.5
4	4374.5
5	6842.5
6	8749.5
7	10350.0
8	11560.5
9	11474.5
10	10503.0
11	9821.0
12	11378.5
13	16146.0
14	26996.0
15	41526.0
16	53152.0
17	59501.0
18	60489.0
19	57876.5
20	54204.0
21	51571.5
22	52167.5
23	58106.0
24	69721.5
25	87228.5
26	109096.5
27	133266.5
28	159315.0
29	186356.0
30	212182.0
31	237073.5
32	263022.5
33	287761.0
34	310424.0
35	331260.0
36	352769.0
37	372097.0
38	386869.0
39	398482.0
40	410075.0
41	421650.0
42	429457.5
43	435237.5
44	438975.5
45	443096.0
46	446248.0
47	437988.0
48	416606.0
49	393894.5
50	373084.5
51	349633.0
52	324625.0
53	300412.5
54	275299.0
55	248204.0
56	222283.5
57	196356.0
58	168839.5
59	142621.5
60	118070.0
61	94914.5
62	73916.5
63	55696.0
64	40636.5
65	29836.5
66	22120.0
67	16504.5
68	12488.0
69	8957.0
70	6370.0
71	4506.5
72	3062.0
73	2027.5
74	1334.5
75	889.0
76	586.5
77	387.0
78	263.0
79	183.5
80	121.5
81	78.5
82	55.5
83	38.0
84	26.0
85	20.0
86	15.0
87	10.0
88	7.0
89	5.0
90	4.0
91	3.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02260545600400611
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	3.453889424881359E-4
12-13	0.0018721081810226497
14-15	4.5050731628887286E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008261143099731184
26-27	0.026843966487743644
28-29	0.3279938859275265
30-31	0.313267220907385
32-33	0.31538120425682575
34-35	0.1686833172577955
36-37	0.028921213380326837
38-39	0.027690729401977702
40-41	0.010443090090324254
42-43	0.007699919642438087
44-45	0.0024798280346282143
46-47	0.0019038611305760257
48-49	0.0017096791219897598
50-51	0.0018969256799565028
52-53	2.1130788691517088E-4
54-55	5.4643175419838545E-5
56-57	1.9304860649518103E-4
58-59	3.1748995339247484E-4
60-61	0.002222212970815199
62-63	0.0016138325910082247
64-65	0.0017456463493912319
66-67	0.006522002495203163
68-69	0.010070986943793905
70-71	0.01445697739007171
72-73	0.013929728334857453
74-75	0.01083259350079608
76-77	0.005335658345945913
78-79	0.002198527533079455
80-81	0.0020368069882093394
82-83	3.2459103597938884E-4
84-85	3.866731550422083E-5
86-87	0.0
88-89	6.223324942437555E-4
90-91	0.0024397899495440683
92-93	0.015575471665245404
94-95	3.754618441420339E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	808.0
20-21	2616.0
22-23	3751.0
24-25	4226.0
26-27	6661.0
28-29	11039.0
30-31	21049.0
32-33	27589.0
34-35	40343.0
36-37	55273.0
38-39	65152.0
40-41	68851.0
42-43	80882.0
44-45	95150.0
46-47	95387.0
48-49	85622.0
50-51	77286.0
52-53	76558.0
54-55	83324.0
56-57	88469.0
58-59	88880.0
60-61	89083.0
62-63	90000.0
64-65	95658.0
66-67	100764.0
68-69	96656.0
70-71	91446.0
72-73	89388.0
74-75	91111.0
76-77	90783.0
78-79	96846.0
80-81	97868.0
82-83	96927.0
84-85	99692.0
86-87	104066.0
88-89	111279.0
90-91	118909.0
92-93	123793.0
94-95	144035.0
96-97	207938.0
98-99	844704.0
100-101	6028877.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.78039072277065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.33959850130888	75.74246067956423
2	7.361733205153731	12.482612350594561
3	1.8583237823945002	4.726482490824683
4	0.6945859856857524	2.355490850279955
5	0.29455060427489244	1.248605765902679
6	0.15652594524648844	0.7962198477748977
7	0.09869234737033673	0.5857023039983189
8	0.051878713046185715	0.35186380498000996
9	0.03828961038959453	0.2921587316507235
>10	0.10446486319626812	1.2837570464763355
>50	0.001013046421077122	0.056198327747428674
>100	3.0634728634470397E-4	0.05154546311279089
>500	3.704822601547131E-5	0.026902337093290207
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.003382108592486E-5	0.0	0.0	0.0
3	0.0	4.004509478123315E-5	0.0	1.0011273695308287E-5	0.0
4	0.0	7.007891586715801E-5	0.0	1.0011273695308287E-5	0.0
5	0.0	8.00901895624663E-5	0.0	1.0011273695308287E-5	0.0
6	0.0	8.00901895624663E-5	0.0	1.0011273695308287E-5	0.0
7	0.0	8.00901895624663E-5	0.0	2.0022547390616574E-5	0.0
8	0.0	9.010146325777459E-5	0.0	2.0022547390616574E-5	0.0
9	0.0	1.0011273695308286E-4	0.0	2.0022547390616574E-5	0.0
10-11	3.003382108592486E-5	1.0011273695308286E-4	0.0	4.5050731628887286E-5	0.0
12-13	3.003382108592486E-5	1.0011273695308286E-4	0.0	1.0011273695308286E-4	5.0056368476541436E-6
14-15	4.004509478123314E-5	1.4015783173431602E-4	0.0	2.202480212967823E-4	1.0011273695308287E-5
16-17	6.006764217184972E-5	1.4015783173431602E-4	0.0	3.5540021618344416E-4	1.0011273695308287E-5
18-19	7.508455271481215E-5	1.5517474227727844E-4	0.0	5.105749584607226E-4	2.0022547390616574E-5
20-21	8.00901895624663E-5	1.601803791249326E-4	0.0	7.808793482340464E-4	2.0022547390616574E-5
22-23	9.010146325777457E-5	1.8020292651554914E-4	0.0	0.001181330296046378	2.0022547390616574E-5
24-25	1.4516346858197017E-4	2.202480212967823E-4	0.0	0.0017519728966789501	2.5028184238270715E-5
26-27	1.6518601597258675E-4	2.4027056868739888E-4	0.0	0.0021223900234053567	3.003382108592486E-5
28-29	1.7019165282024088E-4	2.4527620553505306E-4	0.0	0.003073461024459644	3.003382108592486E-5
30-31	1.7019165282024088E-4	2.953325740115945E-4	0.0	0.004570146441908233	3.003382108592486E-5
32-33	1.7019165282024088E-4	3.003382108592486E-4	0.0	0.006812671749657289	4.5050731628887286E-5
34-35	1.7519728966789501E-4	3.3537766879282763E-4	0.0	0.010046313153241865	5.005636847654143E-5
36-37	1.8020292651554917E-4	3.704171267264066E-4	0.0	0.014616459595150098	5.005636847654143E-5
38-39	1.9021420021085743E-4	3.904396741170232E-4	0.0	0.020978624028518515	5.005636847654143E-5
40-41	2.202480212967823E-4	4.505073162888729E-4	0.0	0.02865727095281997	6.507327901950386E-5
42-43	2.202480212967823E-4	4.705298636794895E-4	0.0	0.035825342918660706	7.508455271481215E-5
44-45	2.302592949920906E-4	5.406087795466474E-4	0.0	0.04414471135946189	8.00901895624663E-5
46-47	2.302592949920906E-4	6.006764217184972E-4	0.0	0.05326998733273539	8.509582641012044E-5
48-49	2.302592949920906E-4	6.056820585661514E-4	0.0	0.06312108064891875	9.010146325777459E-5
50-51	2.302592949920906E-4	6.407215164997304E-4	0.0	0.07228139608012582	9.010146325777459E-5
52-53	2.3526493183974475E-4	6.457271533473845E-4	0.0	0.08074092235266134	1.0511837380073702E-4
54-55	2.4027056868739888E-4	6.65749700738001E-4	0.0	0.0904919029318916	1.2013528434369944E-4
56-57	2.4027056868739888E-4	7.007891586715801E-4	0.0	0.09819057240358367	1.251409211913536E-4
58-59	2.4027056868739888E-4	7.108004323668883E-4	0.0	0.10437753954728421	1.3014655803900773E-4
60-61	2.4027056868739888E-4	7.258173429098508E-4	0.0	0.11091990690716816	1.3014655803900773E-4
62-63	2.4027056868739888E-4	7.30822979757505E-4	0.0	0.116581282181865	1.4015783173431602E-4
64-65	2.4027056868739888E-4	8.40946990405896E-4	0.0	0.12263309713067885	1.4015783173431602E-4
66-67	2.6029311607801545E-4	8.659751746441668E-4	0.0	0.12863485571101618	1.4015783173431602E-4
68-69	2.6029311607801545E-4	8.910033588824375E-4	0.0	0.13495196941275572	1.4015783173431602E-4
70-71	2.652987529256696E-4	9.110259062730541E-4	0.0	0.14064337850853847	1.4015783173431602E-4
72-73	2.703043897733237E-4	9.110259062730541E-4	0.0	0.14549884625076298	1.601803791249326E-4
74-75	2.703043897733237E-4	9.310484536636707E-4	0.0	0.14912793296531224	1.6518601597258675E-4
76-77	2.703043897733237E-4	9.660879115972496E-4	0.0	0.1524616871058499	1.7019165282024088E-4
78-79	2.8031566346863203E-4	9.911160958355203E-4	0.0	0.15534993956694634	1.7019165282024088E-4
80-81	2.8031566346863203E-4	0.0010011273695308288	0.0	0.15689668135287146	1.7519728966789501E-4
82-83	2.8031566346863203E-4	0.0010211499169214453	0.0	0.15734218303231268	1.9021420021085743E-4
84-85	2.8031566346863203E-4	0.0010211499169214453	0.0	0.15761749305893366	1.9021420021085743E-4
86-87	2.8031566346863203E-4	0.0010311611906167536	0.0	0.15770759452219144	1.9021420021085743E-4
88-89	2.8031566346863203E-4	0.0010712062853979866	0.0	0.15776766216436328	2.0022547390616572E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	31245	0.0	23.32571	4
ATCAACG	31355	0.0	23.230398	3
GGTATCA	23440	0.0	23.18136	1
GTATCAA	32850	0.0	23.06726	1
CAACGCA	31600	0.0	23.037027	5
CTTATAC	8330	0.0	22.843409	1
AACGCAG	32415	0.0	22.627468	6
ACGCAGA	37260	0.0	19.61739	7
CGCAGAG	37535	0.0	19.462399	8
GTACATG	23720	0.0	19.288836	1
TATACAC	12980	0.0	18.987686	3
TACATGG	23745	0.0	18.858936	2
ACATGGG	23260	0.0	18.738167	3
TATCAAC	39985	0.0	18.633833	2
AGAGTAC	32215	0.0	18.313053	10-11
GAGTACT	22645	0.0	18.285965	12-13
GCAGAGT	42805	0.0	16.997122	9
TATGCCG	6285	0.0	16.974602	42-43
CAGAGTA	35415	0.0	16.938862	10-11
AGTACTT	24785	0.0	16.75828	12-13
>>END_MODULE