FastQCFastQC Report
Fri 27 May 2016
SRR2139057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4763367
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63700.047.6373981
GTATCAA122750.037.7728731
ATCAACG143950.031.3184093
TCAACGC144100.031.2557684
TATCAAC148800.030.4645422
CAACGCA147300.030.4352785
AACGCAG151100.029.6446486
CGCAGAG170850.026.1946418
ACGCAGA170300.026.1814487
GCAGAGT199200.022.3010739
GAGTACT126350.019.82346512-13
GTACATG131100.019.7044981
TACATGG129100.019.657022
GTGGTAT33150.019.2318331
CAGAGTA192850.018.96716110-11
AGAGTAC181300.018.72541410-11
CTTATAC31400.018.5897251
ACATGGG129700.018.5766123
GTACTTT142000.017.70741714-15
TGGTATC34000.017.1681582
Produced by FastQC (version 0.11.5)