##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139057_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4763367
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59756701509667	33.0	33.0	33.0	33.0	33.0
2	32.61544722462074	33.0	33.0	33.0	33.0	33.0
3	32.670546905161835	33.0	33.0	33.0	33.0	33.0
4	36.578561131233435	37.0	37.0	37.0	37.0	37.0
5	36.578030204265175	37.0	37.0	37.0	37.0	37.0
6	36.08701408058627	37.0	37.0	37.0	33.0	37.0
7	35.915408155617655	37.0	37.0	37.0	33.0	37.0
8	35.83458108518617	37.0	37.0	37.0	33.0	37.0
9	35.83518276042975	37.0	37.0	37.0	33.0	37.0
10-11	35.76602569149092	37.0	37.0	37.0	33.0	37.0
12-13	35.675101981434565	37.0	37.0	37.0	33.0	37.0
14-15	37.33079615742394	40.0	37.0	40.0	33.0	40.0
16-17	37.25458336508608	40.0	37.0	40.0	33.0	40.0
18-19	37.28333938997352	40.0	37.0	40.0	33.0	40.0
20-21	37.209199636729025	40.0	37.0	40.0	33.0	40.0
22-23	37.081590036447835	40.0	37.0	40.0	33.0	40.0
24-25	37.06486295203197	40.0	37.0	40.0	33.0	40.0
26-27	36.89681633233819	40.0	37.0	40.0	33.0	40.0
28-29	36.72098858766788	40.0	37.0	40.0	33.0	40.0
30-31	36.51292572489544	40.0	37.0	40.0	33.0	40.0
32-33	36.29439305318076	38.5	37.0	40.0	33.0	40.0
34-35	36.19864798675495	37.0	37.0	40.0	30.0	40.0
36-37	36.004783337518475	37.0	37.0	40.0	27.0	40.0
38-39	36.043620019297684	37.0	37.0	40.0	27.0	40.0
40-41	36.089206664772604	37.0	37.0	40.0	27.0	40.0
42-43	36.01393005508519	37.0	37.0	40.0	27.0	40.0
44-45	35.8823531269522	37.0	37.0	40.0	27.0	40.0
46-47	35.71304697690064	37.0	37.0	40.0	27.0	40.0
48-49	35.54341381032012	37.0	37.0	40.0	27.0	40.0
50-51	35.55241315815983	37.0	37.0	40.0	27.0	40.0
52-53	35.44522069107296	37.0	33.0	40.0	27.0	40.0
54-55	35.27707776611272	37.0	33.0	40.0	27.0	40.0
56-57	35.03288901906812	37.0	33.0	40.0	27.0	40.0
58-59	34.91577137604533	37.0	33.0	40.0	27.0	40.0
60-61	34.8003142548949	37.0	33.0	40.0	27.0	40.0
62-63	34.65208320248057	37.0	33.0	40.0	27.0	40.0
64-65	34.493153680521274	37.0	33.0	40.0	27.0	40.0
66-67	34.252495709814994	37.0	33.0	40.0	27.0	40.0
68-69	34.073110035636994	37.0	33.0	37.0	27.0	40.0
70-71	33.842128712656184	37.0	33.0	37.0	27.0	40.0
72-73	33.54446411125117	37.0	33.0	37.0	22.0	40.0
74-75	33.18907408794063	37.0	33.0	37.0	22.0	40.0
76-77	31.21613539728262	33.0	30.0	35.0	22.0	37.0
78-79	33.28192464066271	37.0	33.0	37.0	27.0	37.0
80-81	33.6210290895842	37.0	33.0	37.0	27.0	37.0
82-83	33.53785652407194	37.0	33.0	37.0	27.0	37.0
84-85	33.42046966210715	37.0	33.0	37.0	27.0	37.0
86-87	33.27597496071933	37.0	33.0	37.0	27.0	37.0
88-89	33.11124790185322	35.0	33.0	37.0	27.0	37.0
90-91	33.03663770411347	33.0	33.0	37.0	27.0	37.0
92-93	33.08185060521326	33.0	33.0	37.0	27.0	37.0
94-95	33.138802561966514	33.0	33.0	37.0	27.0	37.0
96-97	33.2695644235043	33.0	33.0	37.0	27.0	37.0
98-99	33.43192503344304	33.0	33.0	37.0	27.0	37.0
100	33.40023371684461	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	20.0
17	57.0
18	254.0
19	601.0
20	1631.0
21	4313.0
22	10775.0
23	23790.0
24	43987.0
25	71806.0
26	102051.0
27	131626.0
28	155581.0
29	173288.0
30	184369.0
31	194540.0
32	205646.0
33	226894.0
34	265715.0
35	341887.0
36	531263.0
37	1092886.0
38	980862.0
39	19516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.247882012870306	20.875968616316985	14.928767823264511	22.947381547548197
2	14.153754363262333	21.219452440099417	42.523971044754	22.10282215188425
3	17.81148623739187	27.20591384440195	30.62697818859438	24.355621729611798
4	12.187856441202856	18.65909141963032	39.74768817733669	29.405363961830126
5	12.99990384950474	38.51387863120323	35.633184600973344	12.853032918318686
6	29.165924019711266	38.60174956076238	19.134238449399344	13.098087970127011
7	26.02950811894192	33.24316602100993	23.370464631425627	17.356861228622527
8	24.16626726431115	37.06785557358902	21.442458664217977	17.323418497881857
9	25.565361644399854	17.50215761246194	21.709895542375804	35.2225852007624
10-11	23.938751249773663	27.381161941800563	28.971949961817938	19.708136846607832
12-13	24.89856518002666	24.788777398265754	29.224680343725296	21.087977077982288
14-15	22.03005012903595	26.166789920447513	27.15513653352284	24.648023416993695
16-17	21.363113528728732	29.40889291125374	27.629825709419407	21.598167850598117
18-19	21.35283718428582	28.363613385237795	29.639139709369445	20.64440972110694
20-21	22.197395556111953	27.439019523246795	29.647551895842412	20.716033024798847
22-23	21.952190975400644	27.478993858045026	29.384631362864784	21.184183803689546
24-25	21.72951942917116	27.63252530332571	29.46697891916693	21.170976348336197
26-27	21.747308243748773	27.844877709583148	29.30537541128449	21.10243863538359
28-29	21.574833490749747	27.812617936668133	29.361491738362815	21.251056834219305
30-31	21.84496988666325	27.69479800310531	29.28656058951165	21.17367152071979
32-33	21.64100467994638	27.879433545038594	29.25586954913325	21.223692225881774
34-35	21.69518505399941	27.942864187412592	29.259311662165153	21.10263909642284
36-37	21.966000793684554	27.84679411208829	28.965652412472586	21.22155268175457
38-39	21.74176575289303	27.966702652070463	29.055070665447637	21.236460929588873
40-41	21.90218535893667	27.90678813411883	28.913394485652084	21.277632021292415
42-43	21.916562464476527	27.979004700218944	28.721682128129977	21.382750707174548
44-45	21.97068420876468	28.107474092606726	28.442395492088078	21.479446206540516
46-47	22.04017433163203	28.02987242819283	28.080785993426154	21.849167246748983
48-49	21.926387796556465	28.21922970289187	28.055732529125198	21.79864997142647
50-51	21.878911817420672	28.28203478875387	28.05230182635936	21.786751567466098
52-53	21.994338512897016	28.278902393696274	27.90629068963564	21.82046840377107
54-55	22.01213908871119	28.213311417405507	27.921750753089015	21.852798740794288
56-57	22.044646640372388	28.387090948176123	27.801679476635083	21.7665829348164
58-59	21.924206545609735	28.531757439607368	27.89004703378905	21.653988980993844
60-61	22.07912517461136	28.46014664356501	27.767348725112264	21.69337945671137
62-63	22.01164612228913	28.589150901328825	27.718781625997973	21.68042135038407
64-65	22.000104574292624	28.54575731441461	27.738233157324434	21.71590495396833
66-67	22.030251307941835	28.452828299935167	27.684992822410187	21.831927569712814
68-69	21.97487490777946	28.37549108573418	27.8106947128227	21.83893929366366
70-71	21.992881886654423	28.17008544520483	27.833455892124636	22.00357677601611
72-73	22.037715434492593	28.180665796029388	27.806281544470156	21.97533722500786
74-75	21.951265457919828	28.284309601728737	27.809895133100294	21.954529807251138
76-77	21.99058211930965	28.269620693292495	27.776966041195415	21.96283114620244
78-79	21.835620955594635	28.21522337319185	27.864690710805558	22.084464960407953
80-81	21.923643389273913	28.20220759325346	27.917101642149543	21.957047375323086
82-83	21.91340371461738	28.172593949623543	27.972602528416573	21.9413998073425
84-85	22.040600202790205	28.17352271992948	27.911141019382463	21.874736057897856
86-87	21.88604176709557	28.172112797528737	27.936475528674347	22.005369906701347
88-89	21.947035365420316	28.06893994330306	27.926903752957656	22.057120938318967
90-91	21.93605781455755	28.20861781027803	27.948094697572035	21.907229677592387
92-93	21.94441682096591	28.127327444975787	28.002991747907235	21.925263986151073
94-95	21.828318196823844	28.154356059873937	28.023404056280448	21.99392168702177
96-97	21.796921830856654	28.24516376640263	28.177839646654267	21.78007475608645
98-99	23.003620488870514	30.06628410439224	28.43077546627213	18.49931994046512
100	22.168725699058413	30.931831461184352	22.18985328151134	24.70958955824589
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	586.0
1	577.0
2	1028.5
3	1570.0
4	2296.0
5	4356.5
6	6134.5
7	6901.0
8	6943.5
9	6279.5
10	5436.0
11	4682.5
12	5038.0
13	7249.5
14	12646.5
15	20561.0
16	27813.5
17	32994.0
18	35318.5
19	34365.0
20	31767.5
21	28967.0
22	27484.5
23	28812.5
24	32378.5
25	38339.5
26	47032.0
27	56969.5
28	67709.5
29	79693.5
30	91082.0
31	102661.5
32	115397.5
33	126663.0
34	137688.5
35	148015.0
36	157692.0
37	166414.0
38	172907.5
39	177562.0
40	182664.0
41	188121.0
42	190895.0
43	193400.5
44	197210.5
45	200123.0
46	202143.5
47	203122.0
48	201085.0
49	197554.0
50	194073.0
51	187954.0
52	179030.5
53	168168.5
54	156505.5
55	144208.0
56	130300.5
57	116631.0
58	103174.0
59	89665.5
60	75861.0
61	62693.0
62	51006.5
63	40243.5
64	30904.5
65	23941.0
66	18701.0
67	14713.0
68	11547.0
69	8740.0
70	6544.0
71	4950.5
72	3716.5
73	2841.5
74	2164.5
75	1620.0
76	1233.5
77	918.0
78	671.0
79	489.5
80	362.0
81	265.5
82	189.5
83	121.5
84	78.0
85	55.5
86	47.0
87	34.5
88	18.5
89	9.5
90	6.0
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.358968561523813E-4
3	0.003589897650128575
4	0.0028131361702761933
5	2.099355350952383E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	9.447099079285723E-5
12-13	0.0018159423785738113
14-15	0.003348471784769051
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.005978318701012389
26-27	0.018667290456500677
28-29	0.008050918196111903
30-31	0.0
32-33	0.0
34-35	2.3563401025629164E-4
36-37	0.014363949974260102
38-39	0.03830668845824241
40-41	0.04359528970382988
42-43	0.008280573960186692
44-45	3.989914383087196E-4
46-47	0.0
48-49	0.030962985035386834
50-51	0.046566310677374406
52-53	0.04842987312938464
54-55	0.026578694940793606
56-57	9.733894099454129E-4
58-59	0.007124207535633484
60-61	0.0330108799257411
62-63	0.03792469205113716
64-65	0.01744641318885173
66-67	0.04632592448762859
68-69	0.06212056825791759
70-71	0.07464392998900198
72-73	0.05032953650340877
74-75	0.0218521714789173
76-77	0.00492226724317094
78-79	3.812981413556359E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002051782596047241
92-93	0.002733898539317066
94-95	5.566472886406379E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1382.0
20-21	4460.0
22-23	5010.0
24-25	8842.0
26-27	12253.0
28-29	17823.0
30-31	18702.0
32-33	20535.0
34-35	24314.0
36-37	25280.0
38-39	28139.0
40-41	34145.0
42-43	39685.0
44-45	46747.0
46-47	46848.0
48-49	36208.0
50-51	39436.0
52-53	36733.0
54-55	42236.0
56-57	44032.0
58-59	46453.0
60-61	46184.0
62-63	43574.0
64-65	41075.0
66-67	50980.0
68-69	37603.0
70-71	40737.0
72-73	38853.0
74-75	36772.0
76-77	33832.0
78-79	46897.0
80-81	56863.0
82-83	65051.0
84-85	67156.0
86-87	71965.0
88-89	74468.0
90-91	81760.0
92-93	90853.0
94-95	106971.0
96-97	196047.0
98-99	291700.0
100-101	2664763.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.1303702028457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.49681088496195	80.63647815671459
2	6.1022894553760345	10.755940575744232
3	1.434743608979827	3.7933345621667716
4	0.4778771787875136	1.6846197071214024
5	0.2154342260043377	0.9493149046062906
6	0.10981879953106445	0.5807022874742884
7	0.05436542851103897	0.33538717386399447
8	0.0321839954494829	0.22691099468557038
9	0.021279607857546706	0.16878417464312778
>10	0.053971053600066855	0.6812847683052301
>50	5.060170302547897E-4	0.03146546549496371
>100	7.197439108827244E-4	0.1557772291795499
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.198710701904766E-5
2	0.0	0.0	0.0	0.0	4.198710701904766E-5
3	0.0	0.0	0.0	0.0	4.198710701904766E-5
4	0.0	6.298066052857149E-5	0.0	0.0	6.298066052857149E-5
5	0.0	6.298066052857149E-5	0.0	0.0	6.298066052857149E-5
6	8.397421403809532E-5	8.397421403809532E-5	0.0	0.0	1.0496776754761916E-4
7	8.397421403809532E-5	8.397421403809532E-5	0.0	6.298066052857149E-5	1.0496776754761916E-4
8	8.397421403809532E-5	1.0496776754761916E-4	0.0	6.298066052857149E-5	1.0496776754761916E-4
9	8.397421403809532E-5	1.8894198158571446E-4	0.0	1.0496776754761916E-4	1.0496776754761916E-4
10-11	8.397421403809532E-5	1.8894198158571446E-4	0.0	1.0496776754761916E-4	1.0496776754761916E-4
12-13	8.397421403809532E-5	1.8894198158571446E-4	0.0	1.0496776754761916E-4	1.1546454430238107E-4
14-15	8.397421403809532E-5	3.77883963171429E-4	0.0	1.2596132105714298E-4	1.6794842807619064E-4
16-17	1.0496776754761916E-4	4.1987107019047663E-4	0.0	1.2596132105714298E-4	2.3092908860476214E-4
18-19	1.0496776754761916E-4	4.828517307190481E-4	0.0	1.2596132105714298E-4	2.729161956238098E-4
20-21	1.0496776754761916E-4	5.668259447571435E-4	0.0	1.364580978119049E-4	2.729161956238098E-4
22-23	1.0496776754761916E-4	6.298066052857149E-4	0.0	2.0993553509523832E-4	3.2540007939761933E-4
24-25	1.0496776754761916E-4	7.032840425690483E-4	0.0	2.2043231185000023E-4	3.988775166809528E-4
26-27	1.0496776754761916E-4	7.557679263428579E-4	0.0	2.624194188690479E-4	4.1987107019047663E-4
28-29	1.0496776754761916E-4	7.977550333619056E-4	0.0	6.298066052857149E-4	4.1987107019047663E-4
30-31	1.0496776754761916E-4	8.817292474000009E-4	0.0	0.0015430261829500016	4.1987107019047663E-4
32-33	1.0496776754761916E-4	8.817292474000009E-4	0.0	0.0032749943474857175	4.618581772095243E-4
34-35	1.0496776754761916E-4	9.657034614380961E-4	0.0	0.0053428593681738145	4.618581772095243E-4
36-37	1.0496776754761916E-4	0.0010496776754761914	0.0	0.008848782804264295	4.618581772095243E-4
38-39	1.0496776754761916E-4	0.0010916647824952392	0.0	0.014443564814552395	4.618581772095243E-4
40-41	1.0496776754761916E-4	0.0011546454430238107	0.0	0.019922882280538116	4.618581772095243E-4
42-43	1.0496776754761916E-4	0.0012071293267976201	0.0	0.02504530933686193	4.618581772095243E-4
44-45	1.0496776754761916E-4	0.0013330906478547632	0.0	0.031731756129645274	4.618581772095243E-4
46-47	1.0496776754761916E-4	0.0014275616386476204	0.0	0.03913198374175242	4.7235495396428617E-4
48-49	1.0496776754761916E-4	0.0014380584154023825	0.0	0.04510464971521195	5.668259447571435E-4
50-51	1.2596132105714298E-4	0.0016794842807619065	0.0	0.050006644459685765	5.773227215119054E-4
52-53	1.469548745666668E-4	0.0016794842807619065	0.0	0.05598980720990006	6.088130517761911E-4
54-55	1.469548745666668E-4	0.0016899810575166684	0.0	0.06221439582547387	6.298066052857149E-4
56-57	1.469548745666668E-4	0.0017004778342714303	0.0	0.06850196510157626	6.298066052857149E-4
58-59	1.469548745666668E-4	0.001731968164535716	0.0	0.0736453857114096	6.298066052857149E-4
60-61	1.469548745666668E-4	0.0017634584948000018	0.0	0.07945010325679294	6.298066052857149E-4
62-63	2.0993553509523832E-4	0.001836935932083335	0.0	0.0860735693890477	6.298066052857149E-4
64-65	2.0993553509523832E-4	0.0020573682439333353	0.0	0.09192027404145009	6.298066052857149E-4
66-67	2.3092908860476214E-4	0.0020573682439333353	0.0	0.09803989488947629	6.403033820404768E-4
68-69	2.3092908860476214E-4	0.002109852127707145	0.0	0.10408603830021915	6.612969355500007E-4
70-71	2.3092908860476214E-4	0.002141342457971431	0.0	0.10931343312409059	6.927872658142864E-4
72-73	2.939097491333336E-4	0.0021623360114809546	0.0	0.11568497661423108	7.557679263428579E-4
74-75	2.939097491333336E-4	0.002204323118500002	0.0	0.12132174573153823	7.557679263428579E-4
76-77	3.0440652588809556E-4	0.0022778005557833356	0.0	0.126402185680843	7.662647030976198E-4
78-79	3.1490330264285745E-4	0.002330284439557145	0.0	0.12944625093972395	7.767614798523817E-4
80-81	3.1490330264285745E-4	0.002330284439557145	0.0	0.13045394150818107	8.187485868714294E-4
82-83	3.1490330264285745E-4	0.002330284439557145	0.0	0.1324798194218501	8.817292474000009E-4
84-85	3.1490330264285745E-4	0.002351277993066669	0.0	0.13425377469340488	8.817292474000009E-4
86-87	3.1490330264285745E-4	0.0023722715465761927	0.0	0.13514600071755967	8.817292474000009E-4
88	3.1490330264285745E-4	0.002477239314123812	0.0	0.13526146526186203	8.817292474000009E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6370	0.0	47.637398	1
GTATCAA	12275	0.0	37.772873	1
ATCAACG	14395	0.0	31.318409	3
TCAACGC	14410	0.0	31.255768	4
TATCAAC	14880	0.0	30.464542	2
CAACGCA	14730	0.0	30.435278	5
AACGCAG	15110	0.0	29.644648	6
CGCAGAG	17085	0.0	26.194641	8
ACGCAGA	17030	0.0	26.181448	7
GCAGAGT	19920	0.0	22.301073	9
GAGTACT	12635	0.0	19.823465	12-13
GTACATG	13110	0.0	19.704498	1
TACATGG	12910	0.0	19.65702	2
GTGGTAT	3315	0.0	19.231833	1
CAGAGTA	19285	0.0	18.967161	10-11
AGAGTAC	18130	0.0	18.725414	10-11
CTTATAC	3140	0.0	18.589725	1
ACATGGG	12970	0.0	18.576612	3
GTACTTT	14200	0.0	17.707417	14-15
TGGTATC	3400	0.0	17.168158	2
>>END_MODULE