FastQCFastQC Report
Fri 27 May 2016
SRR2139056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459079
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91100.027.6297551
GTATCAA134550.026.6139321
TCAACGC154400.022.6457234
ATCAACG155000.022.5587123
CAACGCA156550.022.309075
TATCAAC159400.022.1374932
AACGCAG160450.021.7937226
ACGCAGA176750.019.6930357
GAGTACT102000.019.54213712-13
CGCAGAG178600.019.5342868
GTACATG95050.018.9214211
TACATGG94850.018.2418062
AGAGTAC142850.018.01563810-11
GCAGAGT194200.017.6141729
GTACTTT114450.017.51465214-15
ACATGGG96150.017.4106373
AGGACCG6850.016.4090255
AGTACTT109900.016.3291212-13
CAGAGTA182200.016.05288110-11
CATGGGG69600.015.630594
Produced by FastQC (version 0.11.5)