##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139056_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3459079
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54659086999747	33.0	33.0	33.0	33.0	33.0
2	32.54590918565317	33.0	33.0	33.0	33.0	33.0
3	32.634305836900516	33.0	33.0	33.0	33.0	33.0
4	36.52599637071024	37.0	37.0	37.0	37.0	37.0
5	36.56131039505024	37.0	37.0	37.0	37.0	37.0
6	35.902210675153704	37.0	37.0	37.0	33.0	37.0
7	35.696261924055506	37.0	37.0	37.0	33.0	37.0
8	35.56643979510153	37.0	37.0	37.0	33.0	37.0
9	35.530059301912445	37.0	37.0	37.0	33.0	37.0
10-11	35.44045351378213	37.0	37.0	37.0	33.0	37.0
12-13	35.32353583135858	37.0	37.0	37.0	33.0	37.0
14-15	36.781690877831934	38.5	37.0	40.0	33.0	40.0
16-17	36.685912926533334	37.0	37.0	40.0	33.0	40.0
18-19	36.71041684795288	38.5	37.0	40.0	33.0	40.0
20-21	36.630619719123914	38.5	37.0	40.0	33.0	40.0
22-23	36.46643734170997	37.0	37.0	40.0	33.0	40.0
24-25	36.440840530062886	37.0	37.0	40.0	33.0	40.0
26-27	36.24218897018498	37.0	37.0	40.0	27.0	40.0
28-29	36.00378416192114	37.0	37.0	40.0	27.0	40.0
30-31	35.760817801281085	37.0	37.0	40.0	27.0	40.0
32-33	35.511971400351285	37.0	33.0	40.0	27.0	40.0
34-35	35.397712181057344	37.0	33.0	40.0	27.0	40.0
36-37	35.08871887898987	37.0	33.0	40.0	24.5	40.0
38-39	35.159582943547505	37.0	33.0	40.0	27.0	40.0
40-41	35.24300681174887	37.0	33.0	40.0	27.0	40.0
42-43	35.158366821055075	37.0	33.0	40.0	27.0	40.0
44-45	34.98013081091188	37.0	33.0	40.0	24.5	40.0
46-47	34.76890125301346	37.0	33.0	40.0	22.0	40.0
48-49	34.585795222774415	37.0	33.0	40.0	22.0	40.0
50-51	34.642000887605846	37.0	33.0	40.0	22.0	40.0
52-53	34.506625797795266	37.0	33.0	40.0	22.0	40.0
54-55	34.3321714913627	37.0	33.0	40.0	22.0	40.0
56-57	34.03049912433216	37.0	33.0	40.0	22.0	40.0
58-59	33.86392120688227	37.0	33.0	40.0	22.0	40.0
60-61	33.73665431230472	37.0	33.0	40.0	22.0	40.0
62-63	33.57616537012857	37.0	33.0	40.0	22.0	40.0
64-65	33.45326911229334	37.0	33.0	40.0	22.0	40.0
66-67	33.2380379209214	37.0	33.0	37.0	22.0	40.0
68-69	33.09734614502539	37.0	33.0	37.0	22.0	40.0
70-71	32.8497414858421	37.0	33.0	37.0	22.0	40.0
72-73	32.508737716659525	37.0	33.0	37.0	22.0	40.0
74-75	32.09666628747099	37.0	30.0	37.0	22.0	40.0
76-77	30.548479065727232	33.0	27.0	35.0	22.0	37.0
78-79	32.6184010887729	35.0	33.0	37.0	24.5	37.0
80-81	32.97323400895182	37.0	33.0	37.0	27.0	37.0
82-83	32.8557299205792	37.0	33.0	37.0	27.0	37.0
84-85	32.689595697600055	33.0	33.0	37.0	27.0	37.0
86-87	32.54336023049774	33.0	33.0	37.0	27.0	37.0
88-89	32.389793879037946	33.0	33.0	37.0	27.0	37.0
90-91	32.34997345497902	33.0	33.0	37.0	27.0	37.0
92-93	32.41124155877528	33.0	33.0	37.0	27.0	37.0
94-95	32.50996515842212	33.0	33.0	37.0	27.0	37.0
96-97	32.738552091710176	33.0	33.0	37.0	27.0	37.0
98-99	33.0030085560944	33.0	33.0	37.0	27.0	37.0
100	32.96322037904382	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	3.0
15	9.0
16	32.0
17	71.0
18	244.0
19	660.0
20	1786.0
21	4530.0
22	10894.0
23	23310.0
24	42898.0
25	68597.0
26	97520.0
27	123807.0
28	146167.0
29	159914.0
30	166544.0
31	171971.0
32	177042.0
33	187612.0
34	208970.0
35	248914.0
36	346599.0
37	648245.0
38	607281.0
39	15456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34849189625331	21.185899483648683	15.038800790615072	22.426807829482932
2	14.12841739846386	21.723275429422525	42.502393711131106	21.64591346098251
3	17.98966181172702	27.517186948904605	30.713236697099177	23.779914542269196
4	12.1933661982092	18.868364723799743	40.061497329295186	28.876771748695873
5	12.882189993981056	38.832284209838576	35.59165187948927	12.693873916691095
6	28.87751334965174	39.172190054057744	19.014078603003863	12.936217993286652
7	25.69157859649924	33.73782443245731	23.535050803985687	17.035546167057763
8	24.002747552166344	37.58841587601787	21.212033607789817	17.196802964025974
9	25.599733339423587	17.75938045936505	21.9519993616798	34.68888683953156
10-11	23.81131894923947	27.778607318631813	28.964227580461888	19.445846151666828
12-13	24.86202659728245	25.322694420352704	29.31741832899682	20.497860653368026
14-15	21.94229436859612	26.80040815423824	27.308938615400784	23.948358861764856
16-17	21.133558961793007	29.893578608641203	27.87471751873837	21.098144910827422
18-19	21.113510272532082	28.844296415317487	29.95573388176448	20.08645943038595
20-21	21.929274766278894	27.89295818572451	29.9402120070398	20.237555040956792
22-23	21.660351608095247	27.85728643448955	29.782389704668432	20.699972252746775
24-25	21.547824790251312	28.014124727324525	29.801764677041128	20.63628580538304
26-27	21.479619604344535	28.177180158526312	29.757818179068003	20.585382058061157
28-29	21.289213206884952	28.377711055684095	29.615807225762236	20.717268511668717
30-31	21.605578890121247	28.14153024726161	29.585295965808882	20.66759489680826
32-33	21.43215043078354	28.36536780879999	29.424881000020207	20.777600760396265
34-35	21.49360096012308	28.478487450023266	29.440005365672988	20.587906224180667
36-37	21.670328882141412	28.416884394778112	29.25397644875426	20.658810274326214
38-39	21.422780333104328	28.63723588798417	29.32323017628241	20.616753602629093
40-41	21.63337281500799	28.486749105212724	29.136140452579877	20.74373762719941
42-43	21.53391598316299	28.68287344378158	28.81647204409841	20.96673852895701
44-45	21.71629421664169	28.77409835928405	28.49004455888685	21.019562865187403
46-47	21.873606541181356	28.708907346091074	28.191316172753005	21.226169939974564
48-49	21.72267942303605	28.860861474941423	28.17943003618434	21.23702906583819
50-51	21.611994523967773	28.986047953716497	28.1296982762849	21.272259246030835
52-53	21.72437359817391	28.979974110497775	27.896386027868065	21.399266263460255
54-55	21.723999469000084	28.828376977699154	28.02353704834781	21.424086504952953
56-57	21.821085447796058	28.974622782914274	27.83232405100061	21.371967718289056
58-59	21.708218031657335	29.059246353248664	27.96944928982495	21.26308632526905
60-61	21.773372518983937	29.10851846523135	28.00095186984087	21.117157145943853
62-63	21.770676463707336	29.297420478472212	27.796774948227608	21.13512810959284
64-65	21.69554568836633	29.242383341588496	27.880071175751382	21.181999794293795
66-67	21.769474504877472	29.109602881813494	27.816005758499458	21.304916854809576
68-69	21.750180045927063	29.08274455726761	27.88940304210799	21.277672354697337
70-71	21.748682050679236	28.883514980024867	27.938989965541456	21.428813003754442
72-73	21.74710333609074	28.858286004038025	27.966922235608287	21.42768842426295
74-75	21.571762496904515	29.061722437898606	27.852378748349622	21.51413631684726
76-77	21.552727193364515	28.91585037325082	28.01684112239989	21.51458131098478
78-79	21.512729330660793	28.941390504227094	28.07177029169897	21.47410987341315
80-81	21.58184982532482	29.057810587732334	27.9653251912818	21.395014395661054
82-83	21.542976153343695	28.90743937428778	28.130074705256224	21.419509767112302
84-85	21.653192891354163	28.932842573196037	28.023465186190528	21.39049934925927
86-87	21.404009750925972	28.987103437752094	28.14240992815439	21.466476883167545
88-89	21.486588973856115	28.851048611356774	28.170877016485633	21.491485398301478
90-91	21.466170533392066	28.93190278023612	28.237542436828843	21.36438424954297
92-93	21.548725603895917	28.959172037923697	28.169533740328934	21.322568617851456
94-95	21.394527663856262	28.95115022770704	28.2924004098085	21.361921698628198
96-97	21.358704277795773	29.221537789492903	28.445443812418002	20.97431412029332
98-99	22.426640950125908	31.1316863098128	28.645419185881266	17.796253554180026
100	21.57312474203407	31.89211467974595	22.717582134587953	23.81717844363202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	501.0
1	502.0
2	842.5
3	1257.0
4	1888.5
5	3603.0
6	5066.5
7	5649.0
8	5705.0
9	5305.0
10	4741.0
11	4209.5
12	4506.5
13	6350.5
14	10573.0
15	16780.0
16	22729.0
17	27120.5
18	29208.5
19	28693.5
20	27056.0
21	25321.0
22	24190.0
23	24845.0
24	27793.0
25	32954.0
26	39808.0
27	47622.5
28	56002.0
29	65059.5
30	74136.0
31	83061.0
32	92211.0
33	100458.0
34	108704.0
35	115928.0
36	123015.0
37	130141.0
38	134919.5
39	138588.5
40	142517.5
41	145968.5
42	147577.5
43	148507.5
44	148853.0
45	149187.0
46	150125.5
47	151286.0
48	151313.5
49	146818.5
50	140981.5
51	134959.0
52	126931.0
53	118143.5
54	108965.5
55	99539.5
56	89924.0
57	80634.5
58	71118.0
59	61659.5
60	52365.5
61	43611.5
62	35928.0
63	28841.0
64	22643.5
65	17886.0
66	14109.0
67	11216.0
68	8839.5
69	6689.5
70	5128.0
71	3980.0
72	2997.0
73	2242.5
74	1675.5
75	1301.0
76	1021.0
77	778.0
78	582.5
79	419.5
80	294.5
81	216.5
82	168.5
83	117.0
84	79.5
85	56.5
86	41.5
87	33.5
88	22.5
89	12.5
90	8.0
91	5.5
92	3.5
93	2.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.336414403949722E-4
3	0.001705656332220224
4	0.0019369317670975426
5	2.890942935966481E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.0118300275882684E-4
12-13	0.0022838449194135203
14-15	0.0026741222157689952
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0042790702262148605
26-27	0.012957231393030346
28-29	0.00546223427289118
30-31	0.0
32-33	0.0
34-35	2.0774133236387378E-4
36-37	0.02054204096755254
38-39	0.057078398398795964
40-41	0.06569721319155152
42-43	0.010921385021557865
44-45	4.3385729348973886E-4
46-47	0.0
48-49	0.03901053507091632
50-51	0.03535045823774992
52-53	0.046083346463997504
54-55	0.03004477608994949
56-57	8.328029766377113E-4
58-59	0.005924385598173061
60-61	0.027091181837414942
62-63	0.04264745417910406
64-65	0.02711006211450413
66-67	0.07155612147318789
68-69	0.08240643387285158
70-71	0.06821605159866527
72-73	0.03823743728144585
74-75	0.017074979213883703
76-77	0.004042161451898848
78-79	3.8446300418238077E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0016191497955324332
92-93	0.002311641288202161
94-95	7.604832015202059E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1311.0
20-21	4212.0
22-23	4592.0
24-25	8080.0
26-27	12278.0
28-29	16905.0
30-31	17382.0
32-33	19088.0
34-35	22463.0
36-37	23168.0
38-39	25508.0
40-41	31020.0
42-43	35949.0
44-45	42145.0
46-47	42501.0
48-49	32982.0
50-51	36250.0
52-53	33049.0
54-55	38197.0
56-57	39087.0
58-59	38901.0
60-61	39054.0
62-63	36634.0
64-65	35221.0
66-67	43228.0
68-69	33154.0
70-71	36041.0
72-73	34766.0
74-75	33367.0
76-77	30356.0
78-79	44362.0
80-81	50550.0
82-83	61295.0
84-85	61998.0
86-87	60967.0
88-89	61838.0
90-91	67993.0
92-93	76751.0
94-95	88686.0
96-97	142526.0
98-99	194418.0
100-101	1700806.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.5500625080796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.53781426795663	87.49480619876607
2	4.174541975682505	7.72708241584036
3	0.8184699252216688	2.272483282207461
4	0.2416319662407648	0.8945221431813186
5	0.1025653433044844	0.47462144669963385
6	0.048405731444785785	0.26879720825785436
7	0.02564828177974548	0.16616250573581987
8	0.015532444853038625	0.11500229936416194
9	0.00991824556082753	0.08261408219625639
>10	0.024301539227250694	0.32524288832301407
>50	4.251133372341948E-4	0.027505796771242857
>100	7.45165391006294E-4	0.15115973265681987
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.8909429359664813E-5	0.0
2	0.0	0.0	0.0	2.8909429359664813E-5	0.0
3	0.0	0.0	0.0	8.672828807899444E-5	0.0
4	0.0	8.672828807899444E-5	0.0	1.1563771743865925E-4	0.0
5	0.0	8.672828807899444E-5	0.0	1.4454714679832406E-4	0.0
6	0.0	8.672828807899444E-5	0.0	1.4454714679832406E-4	0.0
7	0.0	8.672828807899444E-5	0.0	1.734565761579889E-4	0.0
8	0.0	8.672828807899444E-5	0.0	1.734565761579889E-4	0.0
9	0.0	1.1563771743865925E-4	0.0	1.734565761579889E-4	0.0
10-11	0.0	1.1563771743865925E-4	0.0	2.168207201974861E-4	0.0
12-13	0.0	1.3009243211849165E-4	0.0	2.457301495571509E-4	1.4454714679832407E-5
14-15	0.0	1.5900186147815647E-4	0.0	2.601848642369833E-4	2.8909429359664813E-5
16-17	0.0	1.734565761579889E-4	0.0	2.601848642369833E-4	2.8909429359664813E-5
18-19	5.781885871932963E-5	1.734565761579889E-4	0.0	3.469131523159778E-4	4.336414403949722E-5
20-21	5.781885871932963E-5	1.734565761579889E-4	0.0	4.0473201103530737E-4	5.781885871932963E-5
22-23	5.781885871932963E-5	2.3127543487731848E-4	0.0	4.770055844344694E-4	5.781885871932963E-5
24-25	5.781885871932963E-5	3.0354900827648054E-4	0.0	5.492791578336314E-4	5.781885871932963E-5
26-27	5.781885871932963E-5	3.1800372295631295E-4	0.0	7.660998780311176E-4	1.1563771743865925E-4
28-29	5.781885871932963E-5	3.1800372295631295E-4	0.0	0.0011997413184260896	1.1563771743865925E-4
30-31	1.1563771743865925E-4	3.7582258167564254E-4	0.0	0.002500665639611006	1.1563771743865925E-4
32-33	1.1563771743865925E-4	3.7582258167564254E-4	0.0	0.00540606329025732	1.1563771743865925E-4
34-35	1.1563771743865925E-4	3.9027729635547495E-4	0.0	0.008456008087701958	1.1563771743865925E-4
36-37	1.4454714679832406E-4	4.0473201103530737E-4	0.0	0.013052607355888663	1.1563771743865925E-4
38-39	1.4454714679832406E-4	4.0473201103530737E-4	0.0	0.02108942871787548	1.1563771743865925E-4
40-41	1.734565761579889E-4	4.336414403949722E-4	0.0	0.029097340650502634	1.1563771743865925E-4
42-43	1.734565761579889E-4	4.6255086975463696E-4	0.0	0.03769789588500291	1.1563771743865925E-4
44-45	1.734565761579889E-4	5.059150137941342E-4	0.0	0.0472380075736923	1.1563771743865925E-4
46-47	1.734565761579889E-4	5.203697284739666E-4	0.0	0.05692266640918002	1.1563771743865925E-4
48-49	1.734565761579889E-4	5.203697284739666E-4	0.0	0.06598577251343493	1.1563771743865925E-4
50-51	1.734565761579889E-4	5.492791578336314E-4	0.0	0.07357449772034694	1.1563771743865925E-4
52-53	1.734565761579889E-4	5.492791578336314E-4	0.0	0.0826954226833212	1.1563771743865925E-4
54-55	1.734565761579889E-4	5.492791578336314E-4	0.0	0.09213435136925176	1.3009243211849165E-4
56-57	1.734565761579889E-4	5.492791578336314E-4	0.0	0.10020008216059824	1.4454714679832406E-4
58-59	1.734565761579889E-4	5.492791578336314E-4	0.0	0.10706607163351864	1.4454714679832406E-4
60-61	1.734565761579889E-4	5.492791578336314E-4	0.0	0.11329605366052639	1.4454714679832406E-4
62-63	2.0236600551765368E-4	5.492791578336314E-4	0.0	0.12134732973719305	1.4454714679832406E-4
64-65	2.0236600551765368E-4	5.781885871932963E-4	0.0	0.12789531548715716	1.4454714679832406E-4
66-67	2.0236600551765368E-4	5.781885871932963E-4	0.0	0.13486248796283634	1.4454714679832406E-4
68-69	2.0236600551765368E-4	5.926433018731286E-4	0.0	0.14298603761290218	1.4454714679832406E-4
70-71	2.0236600551765368E-4	6.070980165529611E-4	0.0	0.14840655561783933	1.734565761579889E-4
72-73	2.0236600551765368E-4	6.070980165529611E-4	0.0	0.15677583541746226	1.734565761579889E-4
74-75	2.0236600551765368E-4	6.070980165529611E-4	0.0	0.16372855317846166	1.734565761579889E-4
76-77	2.0236600551765368E-4	6.215527312327935E-4	0.0	0.1693514371889165	1.734565761579889E-4
78-79	2.0236600551765368E-4	6.360074459126259E-4	0.0	0.17263265742123843	1.734565761579889E-4
80-81	2.0236600551765368E-4	6.360074459126259E-4	0.0	0.17436722318281833	1.879112908378213E-4
82-83	2.0236600551765368E-4	6.360074459126259E-4	0.0	0.17753280569770163	2.0236600551765368E-4
84-85	2.0236600551765368E-4	6.649168752722907E-4	0.0	0.17990337890519414	2.0236600551765368E-4
86-87	2.0236600551765368E-4	6.649168752722907E-4	0.0	0.1813633050878572	2.0236600551765368E-4
88	2.0236600551765368E-4	6.649168752722907E-4	0.0	0.1814933975199757	2.0236600551765368E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9110	0.0	27.629755	1
GTATCAA	13455	0.0	26.613932	1
TCAACGC	15440	0.0	22.645723	4
ATCAACG	15500	0.0	22.558712	3
CAACGCA	15655	0.0	22.30907	5
TATCAAC	15940	0.0	22.137493	2
AACGCAG	16045	0.0	21.793722	6
ACGCAGA	17675	0.0	19.693035	7
GAGTACT	10200	0.0	19.542137	12-13
CGCAGAG	17860	0.0	19.534286	8
GTACATG	9505	0.0	18.921421	1
TACATGG	9485	0.0	18.241806	2
AGAGTAC	14285	0.0	18.015638	10-11
GCAGAGT	19420	0.0	17.614172	9
GTACTTT	11445	0.0	17.514652	14-15
ACATGGG	9615	0.0	17.410637	3
AGGACCG	685	0.0	16.409025	5
AGTACTT	10990	0.0	16.32912	12-13
CAGAGTA	18220	0.0	16.052881	10-11
CATGGGG	6960	0.0	15.63059	4
>>END_MODULE