FastQCFastQC Report
Fri 27 May 2016
SRR2139055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11613146
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171150.040.4852871
GTATCAA295800.033.762241
CTTATAC90800.030.7214721
TCAACGC345850.028.2294144
ATCAACG344800.028.2165933
CAACGCA349850.027.8094125
AACGCAG361650.026.9963426
TATCAAC373600.026.3438762
ACGCAGA419150.023.242437
CGCAGAG419700.023.2322588
TATACAC151400.022.5826683
TTATACA152250.020.2240242
TACATGG312550.020.1527942
GTACATG320750.020.062471
GCAGAGT489550.019.778279
ACATGGG320400.019.0697483
GAGTACT282500.018.77983312-13
ACATCTC153550.018.7175458
AGAGTAC427200.018.64822410-11
ACACATC164000.017.8361956
Produced by FastQC (version 0.11.5)