##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139055_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11613146
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44635286596758	34.0	31.0	34.0	31.0	34.0
2	32.58640475199399	34.0	31.0	34.0	31.0	34.0
3	32.646388584109765	34.0	31.0	34.0	31.0	34.0
4	36.18128395182494	37.0	35.0	37.0	35.0	37.0
5	36.094120490692184	37.0	35.0	37.0	35.0	37.0
6	36.12971980202436	37.0	36.0	37.0	35.0	37.0
7	36.079549159202855	37.0	35.0	37.0	35.0	37.0
8	36.052547173694364	37.0	35.0	37.0	35.0	37.0
9	37.763629424791525	39.0	38.0	39.0	35.0	39.0
10-11	37.68814544310388	39.0	37.0	39.0	35.0	39.0
12-13	37.63282425795732	39.0	37.0	39.0	35.0	39.0
14-15	38.96540304410192	40.0	38.0	41.0	35.0	41.0
16-17	38.933899005489124	40.0	38.0	41.0	35.0	41.0
18-19	38.92460837054834	40.0	38.0	41.0	35.0	41.0
20-21	38.89272750156859	40.0	38.0	41.0	35.0	41.0
22-23	38.78726754239328	40.0	38.0	41.0	34.5	41.0
24-25	38.661009224332616	40.0	38.0	41.0	34.0	41.0
26-27	38.589599766052565	40.0	38.0	41.0	34.0	41.0
28-29	38.53638710821206	40.0	38.0	41.0	34.0	41.0
30-31	38.463025615229604	40.0	38.0	41.0	34.0	41.0
32-33	38.358818870611074	40.0	38.0	41.0	34.0	41.0
34-35	38.24729486970385	40.0	38.0	41.0	34.0	41.0
36-37	38.38827319073404	40.0	38.0	41.0	34.0	41.0
38-39	38.44913823359782	40.0	38.0	41.0	34.0	41.0
40-41	38.3917938327239	40.0	38.0	41.0	34.0	41.0
42-43	38.35788337956314	40.0	38.0	41.0	34.0	41.0
44-45	38.325503624517225	40.0	38.0	41.0	33.5	41.0
46-47	38.234105460289385	40.0	38.0	41.0	33.0	41.0
48-49	38.155486906418446	40.0	38.0	41.0	33.0	41.0
50-51	38.07923694998169	40.0	37.0	41.0	33.0	41.0
52-53	38.004954539211326	40.0	37.0	41.0	33.0	41.0
54-55	37.87516667839405	40.0	37.0	41.0	33.0	41.0
56-57	37.71632808388809	40.0	36.5	41.0	33.0	41.0
58-59	37.588132119529945	39.0	36.0	41.0	33.0	41.0
60-61	37.434959146154874	39.0	36.0	41.0	32.0	41.0
62-63	37.21172406288757	39.0	35.0	40.5	32.0	41.0
64-65	36.88652878512238	38.5	35.0	40.0	31.5	41.0
66-67	36.58771151130588	38.0	35.0	40.0	31.0	41.0
68-69	36.31039036096654	37.0	35.0	40.0	31.0	41.0
70-71	36.00135620528551	37.0	35.0	39.5	31.0	41.0
72-73	35.69212221967138	36.0	34.0	39.0	30.5	41.0
74-75	35.366436186777634	36.0	34.0	39.0	30.0	40.0
76-77	34.387490123117665	35.0	33.5	37.0	29.0	39.0
78-79	34.71496461950011	35.0	34.0	37.0	30.0	39.0
80-81	34.56376767591422	35.0	34.0	37.0	30.0	39.0
82-83	34.32621763647799	35.0	34.0	36.5	30.0	39.0
84-85	34.067388243693074	35.0	34.0	36.0	30.0	37.0
86-87	33.83901027668895	35.0	34.0	36.0	30.0	37.0
88-89	33.63225099378012	35.0	33.0	35.0	29.0	37.0
90-91	33.46400063333759	35.0	33.0	35.0	29.0	36.0
92-93	33.336446764842094	35.0	33.0	35.0	29.0	36.0
94-95	33.27597068226183	35.0	33.0	35.0	29.0	36.0
96-97	33.29575107945092	35.0	33.0	35.0	29.0	35.5
98-99	33.34503493127342	35.0	33.0	35.0	30.0	35.0
100-101	31.870445686654314	33.5	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	61.0
21	357.0
22	1834.0
23	6085.0
24	15427.0
25	31561.0
26	55469.0
27	86699.0
28	122920.0
29	163675.0
30	209212.0
31	260654.0
32	325258.0
33	417837.0
34	570258.0
35	805953.0
36	1313505.0
37	2450909.0
38	3184141.0
39	1518624.0
40	72700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.96929789740007	21.833386060934735	15.919588025501444	24.277728016163753
2	13.49548227041126	21.660520781314947	41.74088267511466	23.10311427315912
3	16.874927775815443	26.725936279454338	31.399562185819413	24.999573758910806
4	11.931194182868277	19.033335153110105	38.997511957569465	30.037958706452155
5	12.751213151027294	38.11055161107937	35.985838807158714	13.152396430734617
6	29.299846914866993	38.850962521266844	18.68913901538825	13.160051548477906
7	25.79769512929571	33.026718169219606	23.359828594249997	17.81575810723468
8	23.927366451778013	37.54539037053353	21.140834705772235	17.386408471916223
9	24.926690838124312	17.204433665089546	21.63964011130145	36.22923538548469
10-11	23.683861215969866	27.344986917154866	29.06376014656196	19.9073917203133
12-13	24.54569455970134	24.70768617130518	29.286433495304852	21.460185773688625
14-15	21.642113665616346	26.235366525468883	26.940076727724644	25.182443081190126
16-17	21.173874848383033	29.521432004729814	27.57796638395832	21.726726762928838
18-19	21.181017615726176	28.51958461557273	29.432317478829596	20.867080289871495
20-21	21.94898057554536	27.543756034095736	29.42228846795636	21.084974922402548
22-23	21.600804862694893	27.72104604074531	29.193376538781234	21.484772557778562
24-25	21.5372791962501	27.840545851654408	29.310275018351582	21.31189993374391
26-27	21.57313972695688	27.947916871378325	29.1934774296559	21.28546597200889
28-29	21.27741341823805	27.96132003014071	29.292573566377534	21.46869298524371
30-31	21.638522303945436	27.730240166027155	29.36451151392963	21.266726016097778
32-33	21.399356175968155	27.975464568200092	29.181348107991305	21.44383114784045
34-35	21.41455453118129	28.044611868203457	29.29906753846415	21.241766062151097
36-37	21.660501039128675	27.877667383823063	29.064941075368534	21.39689050167973
38-39	21.470157468815763	27.89243473307757	29.178369869298727	21.459037928807945
40-41	21.664072733406357	27.846439621490997	29.051189439406944	21.438298205695702
42-43	21.67188764276972	27.949938874860248	28.88674228684973	21.491431195520295
44-45	21.66690388345999	28.144670097168063	28.576675040359717	21.61175097901223
46-47	21.85054592100463	27.966875390962	28.26047597105682	21.922102716976553
48-49	21.774273263884368	28.098237590230074	28.278881678327945	21.848607467557617
50-51	21.65156023490806	28.22658555312392	28.244818632252667	21.877035579715358
52-53	21.730549816092555	28.224799422257323	28.238729270815394	21.805921490834727
54-55	21.7698614512804	28.18853319836332	28.11639571997717	21.925209630379115
56-57	21.97613615314834	28.311030922171078	27.906124330302912	21.806708594377668
58-59	21.69934452904291	28.514302706064797	28.155725350125405	21.63062741476689
60-61	21.954411968172604	28.55088128446478	27.873324920203807	21.62138182715881
62-63	21.81725462473299	28.691751648464546	27.775774121148977	21.715219605653484
64-65	21.76897016462794	28.58424153847377	27.870514467587874	21.776273829310412
66-67	21.85685554886432	28.481793321951344	27.791336941969163	21.870014187215173
68-69	21.84807562291822	28.362707915944636	27.86506462314532	21.924151837991825
70-71	21.83036144934984	28.20058808939279	27.934763208275164	22.034287252982203
72-73	21.93018579740603	28.164707437656343	27.942992070461486	21.962114694476142
74-75	21.80823477522132	28.323592570511728	27.906791680735317	21.961380973531632
76-77	21.910172395368317	28.262042255161607	27.815826010345624	22.011959339124452
78-79	21.784796395070266	28.31484673073638	27.805393671104422	22.094963203088927
80-81	21.8348111050547	28.279776785266307	27.865295070756474	22.020117038922518
82-83	21.871238416475403	28.251758633506473	27.963774889512656	21.913228060505467
84-85	22.001817591520876	28.23795619019101	27.858876314775316	21.9013499035128
86-87	21.811532879840893	28.276559852421673	27.904558738810643	22.007348528926787
88-89	21.886663635409747	28.222274142212584	27.893706411108393	21.99735581126928
90-91	21.887984629943507	28.29954449591858	27.899732808165524	21.91273806597239
92-93	21.9129504361316	28.253224975991316	27.912946633688346	21.92087795418874
94-95	21.69596954344268	28.35102737650374	27.98125932303705	21.971743757016526
96-97	21.669336675789935	28.402791518592068	28.02816952752163	21.899702278096367
98-99	21.92217431622873	28.922080230633785	28.06880057451445	21.086944878623033
100-101	22.13331061077693	31.7906192043126	25.3326399361878	20.74343024872267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2783.0
1	2426.0
2	3202.5
3	4573.0
4	6209.0
5	9483.5
6	12012.5
7	14199.0
8	15776.0
9	15464.5
10	13992.0
11	12761.5
12	14311.5
13	20128.5
14	34041.0
15	52736.5
16	67328.5
17	74951.0
18	75794.0
19	71772.0
20	65844.5
21	60360.5
22	58500.5
23	63805.5
24	75194.5
25	92869.5
26	115780.5
27	140807.5
28	168107.0
29	196957.5
30	222751.5
31	248534.5
32	276542.5
33	303228.5
34	329884.5
35	353182.5
36	377897.0
37	401060.0
38	415428.0
39	426814.5
40	440319.5
41	451293.5
42	456301.0
43	462763.5
44	471500.5
45	482126.5
46	493918.5
47	497398.5
48	485591.0
49	471394.5
50	458215.5
51	437066.5
52	410412.0
53	383099.0
54	357357.0
55	328382.0
56	297981.5
57	266535.0
58	231752.5
59	198130.5
60	165339.0
61	134068.0
62	105844.5
63	79817.5
64	58189.5
65	43365.0
66	32920.0
67	24908.0
68	18741.0
69	13628.0
70	9764.5
71	6929.0
72	4815.5
73	3220.5
74	2150.0
75	1416.5
76	900.0
77	577.0
78	401.0
79	284.0
80	204.0
81	155.0
82	116.5
83	83.0
84	58.0
85	42.0
86	30.0
87	24.5
88	23.5
89	18.5
90	13.5
91	11.5
92	8.0
93	6.0
94	5.0
95	4.5
96	4.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.022973964160960345
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	3.2290991605547715E-4
12-13	0.001713575287867732
14-15	3.013825883184453E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.00829101577347446
26-27	0.026153153729961062
28-29	0.3236739456765827
30-31	0.30888042887244704
32-33	0.31084584822055966
34-35	0.1671896358824124
36-37	0.0306393490708845
38-39	0.029343991989620368
40-41	0.011661194490905944
42-43	0.009044885019786796
44-45	0.00313241834751085
46-47	0.0020911220463014253
48-49	0.0018187922643566115
50-51	0.001967990791093583
52-53	3.20492320794977E-4
54-55	7.95960379339734E-5
56-57	4.251614515182019E-4
58-59	7.963130040345412E-4
60-61	0.0032397875565873665
62-63	0.002770104776540264
64-65	0.0027780640918037293
66-67	0.006534236172598699
68-69	0.009756267862873725
70-71	0.013684762771849714
72-73	0.0130391752729319
74-75	0.010231795405039762
76-77	0.005013883757492231
78-79	0.0022221010593616087
80-81	0.002113650338184054
82-83	2.699249851473777E-4
84-85	1.6380598622424416E-5
86-87	0.0
88-89	5.764018400537498E-4
90-91	0.0022244683804303984
92-93	0.014251369620561604
94-95	4.509060517916547E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	272.0
20-21	994.0
22-23	2003.0
24-25	3944.0
26-27	6498.0
28-29	10248.0
30-31	23107.0
32-33	30357.0
34-35	44237.0
36-37	62274.0
38-39	73458.0
40-41	79044.0
42-43	92419.0
44-45	109618.0
46-47	108674.0
48-49	95578.0
50-51	85074.0
52-53	84011.0
54-55	92282.0
56-57	98742.0
58-59	99071.0
60-61	98753.0
62-63	99653.0
64-65	105891.0
66-67	112520.0
68-69	104291.0
70-71	99520.0
72-73	95786.0
74-75	97300.0
76-77	98433.0
78-79	104777.0
80-81	106457.0
82-83	104566.0
84-85	105973.0
86-87	112846.0
88-89	121319.0
90-91	129695.0
92-93	137603.0
94-95	158994.0
96-97	249544.0
98-99	1015310.0
100-101	7152010.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.00221037342945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.29897554052906	70.71409982118807
2	8.250995809175402	13.366977966502214
3	2.3335242629219213	5.6706186977011015
4	0.9336063598960314	3.024967150810801
5	0.44516562819545163	1.8029699933053926
6	0.25691549716536166	1.2486433889749708
7	0.1447270769221196	0.8206249192103929
8	0.09188287170816963	0.5954172563055988
9	0.060286928323072794	0.4395037005714065
>10	0.18142908246456865	2.128330795478626
>50	0.002105693820423577	0.113152560192827
>100	3.4637018326879557E-4	0.049043382911863644
>500	1.944028165690148E-5	0.0091017994343583
>1k	1.943841354868933E-5	0.016548567412400843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.610931094812723E-6
2	0.0	8.610931094812723E-6	0.0	0.0	8.610931094812723E-6
3	0.0	8.610931094812723E-6	0.0	0.0	8.610931094812723E-6
4	0.0	8.610931094812723E-5	0.0	1.7221862189625447E-5	8.610931094812723E-6
5	0.0	1.0333117313775269E-4	0.0	1.7221862189625447E-5	1.7221862189625447E-5
6	0.0	1.0333117313775269E-4	0.0	1.7221862189625447E-5	1.7221862189625447E-5
7	0.0	1.0333117313775269E-4	0.0	4.3054655474063615E-5	1.7221862189625447E-5
8	0.0	1.2916396642219085E-4	0.0	4.3054655474063615E-5	2.5832793284438172E-5
9	0.0	1.9805141518069265E-4	0.0	5.1665586568876344E-5	5.1665586568876344E-5
10-11	0.0	1.9805141518069265E-4	0.0	6.458198321109542E-5	5.1665586568876344E-5
12-13	0.0	1.9805141518069265E-4	0.0	8.610931094812723E-5	5.597105211628271E-5
14-15	0.0	3.0138258831844533E-4	0.0	1.0333117313775269E-4	6.458198321109542E-5
16-17	0.0	3.0138258831844533E-4	0.0	1.119421042325654E-4	8.610931094812723E-5
18-19	0.0	3.444372437925089E-4	0.0	1.54996759706629E-4	1.0763663868515905E-4
20-21	0.0	3.831864337191662E-4	0.0	2.1096781182291174E-4	1.2055303532737813E-4
22-23	0.0	4.1763015809841706E-4	0.0	2.583279328443817E-4	1.2485850087478448E-4
24-25	0.0	4.5637934802507434E-4	0.0	3.1860445050807074E-4	1.6360769080144173E-4
26-27	0.0	4.865176068569189E-4	0.0	4.004082959087916E-4	1.7221862189625446E-4
28-29	0.0	4.951285379517316E-4	0.0	6.329034354687352E-4	1.7652408744366083E-4
30-31	0.0	5.252667967835761E-4	0.0	0.001274417802032283	1.808295529910672E-4
32-33	0.0	5.338777278783888E-4	0.0	0.0027770252780771033	1.8944048408587992E-4
34-35	0.0	6.544307632057669E-4	0.0	0.00447337870375521	1.8944048408587992E-4
36-37	8.610931094812723E-6	7.749837985331451E-4	0.0	0.007388178879349316	1.9374594963328628E-4
38-39	8.610931094812723E-6	7.879001951753642E-4	0.0	0.011900306773031184	2.0666234627550537E-4
40-41	8.610931094812723E-6	8.783149716708978E-4	0.0	0.017092698223203258	2.1096781182291174E-4
42-43	8.610931094812723E-6	9.213696271449614E-4	0.0	0.021566076926958465	2.1527327737031808E-4
44-45	8.610931094812723E-6	0.0010505335935671522	0.0	0.027176098535228954	2.1527327737031808E-4
46-47	8.610931094812723E-6	0.001140948370062686	0.0	0.033896930254730284	2.2818967401253717E-4
48-49	8.610931094812723E-6	0.0011452538356100921	0.0	0.04040679416240871	2.3249513955994353E-4
50-51	8.610931094812723E-6	0.001261501405390064	0.0	0.04671430118935903	2.3249513955994353E-4
52-53	8.610931094812723E-6	0.0012658068709374703	0.0	0.05253529060945243	2.368006051073499E-4
54-55	8.610931094812723E-6	0.0012701123364848767	0.0	0.0588815468263294	2.4110607065475626E-4
56-57	8.610931094812723E-6	0.0012830287331270958	0.0	0.06522349757765897	2.454115362021626E-4
58-59	8.610931094812723E-6	0.0012916396642219086	0.0	0.07088949023804575	2.583279328443817E-4
60-61	8.610931094812723E-6	0.001321777923053753	0.0	0.07652103917405327	2.669388639391944E-4
62-63	8.610931094812723E-6	0.0013777489751700357	0.0	0.08162301584772981	2.798552605814135E-4
64-65	8.610931094812723E-6	0.0015585785281611029	0.0	0.08718567733497883	2.841607261288199E-4
66-67	8.610931094812723E-6	0.0015671894592559157	0.0	0.09267945137346933	2.841607261288199E-4
68-69	8.610931094812723E-6	0.0016274659769196047	0.0	0.09819475273969688	3.0138258831844533E-4
70-71	8.610931094812723E-6	0.0016705206323936682	0.0	0.10370144317482963	3.186044505080708E-4
72-73	8.610931094812723E-6	0.0016834370290358873	0.0	0.10830829131055443	3.358263126976962E-4
74-75	8.610931094812723E-6	0.0017178807534151382	0.0	0.11188182771490171	3.5304817488732165E-4
76-77	8.610931094812723E-6	0.0018513501853847356	0.0	0.1152616181696157	3.616591059821344E-4
78-79	8.610931094812723E-6	0.0018987103064062056	0.0	0.11839599708812754	3.616591059821344E-4
80-81	8.610931094812723E-6	0.0019030157719536118	0.0	0.11964888756242278	3.917973648139789E-4
82-83	8.610931094812723E-6	0.0019159321685958308	0.0	0.12004929585833157	4.348520202880425E-4
84-85	8.610931094812723E-6	0.0019546813585224884	0.0	0.12037220577438706	4.391574858354489E-4
86-87	8.610931094812723E-6	0.0019891250829017393	0.0	0.12048845334416704	4.391574858354489E-4
88-89	8.610931094812723E-6	0.0021268999804187425	0.0	0.12057025718956775	4.477684169302616E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17115	0.0	40.485287	1
GTATCAA	29580	0.0	33.76224	1
CTTATAC	9080	0.0	30.721472	1
TCAACGC	34585	0.0	28.229414	4
ATCAACG	34480	0.0	28.216593	3
CAACGCA	34985	0.0	27.809412	5
AACGCAG	36165	0.0	26.996342	6
TATCAAC	37360	0.0	26.343876	2
ACGCAGA	41915	0.0	23.24243	7
CGCAGAG	41970	0.0	23.232258	8
TATACAC	15140	0.0	22.582668	3
TTATACA	15225	0.0	20.224024	2
TACATGG	31255	0.0	20.152794	2
GTACATG	32075	0.0	20.06247	1
GCAGAGT	48955	0.0	19.77827	9
ACATGGG	32040	0.0	19.069748	3
GAGTACT	28250	0.0	18.779833	12-13
ACATCTC	15355	0.0	18.717545	8
AGAGTAC	42720	0.0	18.648224	10-11
ACACATC	16400	0.0	17.836195	6
>>END_MODULE