FastQCFastQC Report
Fri 27 May 2016
SRR2139054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12032010
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC92750.033.8600921
GGTATCA277800.030.6328491
GTATCAA422500.028.453941
TATACAC131950.026.7065493
TCAACGC463600.025.043194
ATCAACG464900.024.963983
TTATACA133600.024.7391322
CAACGCA472550.024.5693875
AACGCAG483650.024.1280756
TATCAAC521900.022.711142
GTACATG398350.021.5957581
TACACAT163100.020.9854565
ACGCAGA556000.020.9435397
TACATGG406800.020.8670122
CGCAGAG570850.020.3691438
ACATGGG418850.019.8292923
GAGTACT320850.018.9877512-13
AGAGTAC510900.018.91785210-11
ACACATC176600.018.6407176
ACATCTC175250.018.592278
Produced by FastQC (version 0.11.5)