##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139054_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12032010
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4299939079173	34.0	31.0	34.0	31.0	34.0
2	32.5663028870488	34.0	31.0	34.0	31.0	34.0
3	32.62066845024231	34.0	31.0	34.0	31.0	34.0
4	36.16692614118506	37.0	35.0	37.0	35.0	37.0
5	36.075667157856415	37.0	35.0	37.0	35.0	37.0
6	36.11219870994123	37.0	35.0	37.0	35.0	37.0
7	36.05671571084133	37.0	35.0	37.0	35.0	37.0
8	36.0297266209054	37.0	35.0	37.0	35.0	37.0
9	37.730311228132294	39.0	38.0	39.0	35.0	39.0
10-11	37.651653547495386	39.0	37.0	39.0	35.0	39.0
12-13	37.591861251777544	39.0	37.0	39.0	35.0	39.0
14-15	38.90671724840654	40.0	38.0	41.0	35.0	41.0
16-17	38.8693336774155	40.0	38.0	41.0	35.0	41.0
18-19	38.86163434039699	40.0	38.0	41.0	35.0	41.0
20-21	38.82589783989424	40.0	38.0	41.0	35.0	41.0
22-23	38.71677061733901	40.0	38.0	41.0	34.0	41.0
24-25	38.57919788468361	40.0	38.0	41.0	34.0	41.0
26-27	38.492040999455625	40.0	38.0	41.0	34.0	41.0
28-29	38.43312449442469	40.0	38.0	41.0	34.0	41.0
30-31	38.35351447928102	40.0	38.0	41.0	34.0	41.0
32-33	38.24031153928024	40.0	38.0	41.0	34.0	41.0
34-35	38.12453134214896	40.0	38.0	41.0	33.5	41.0
36-37	38.27579135228687	40.0	38.0	41.0	34.0	41.0
38-39	38.33098336018586	40.0	38.0	41.0	34.0	41.0
40-41	38.26432736134701	40.0	38.0	41.0	33.5	41.0
42-43	38.220952799202735	40.0	38.0	41.0	33.0	41.0
44-45	38.1784928396184	40.0	38.0	41.0	33.0	41.0
46-47	38.06694634686529	40.0	37.0	41.0	33.0	41.0
48-49	37.96656089114722	40.0	37.0	41.0	33.0	41.0
50-51	37.86505092457638	40.0	37.0	41.0	33.0	41.0
52-53	37.767312357267244	40.0	36.5	41.0	33.0	41.0
54-55	37.60534033475831	39.5	36.0	41.0	32.5	41.0
56-57	37.41051895007021	39.0	36.0	41.0	32.0	41.0
58-59	37.24780642371291	39.0	35.0	41.0	32.0	41.0
60-61	37.05448354629745	39.0	35.0	40.5	32.0	41.0
62-63	36.79027201672423	38.0	35.0	40.0	31.0	41.0
64-65	36.427569098780694	37.5	35.0	40.0	31.0	41.0
66-67	36.09574966593088	37.0	35.0	40.0	31.0	41.0
68-69	35.78728045678082	36.5	34.5	39.0	31.0	41.0
70-71	35.46264968675001	36.0	34.0	39.0	30.0	40.5
72-73	35.14756766797241	35.5	34.0	38.5	30.0	40.0
74-75	34.83095651073245	35.0	34.0	37.5	30.0	39.5
76-77	33.900316852614964	35.0	32.5	36.0	29.0	39.0
78-79	34.24309648277264	35.0	33.5	36.5	29.5	39.0
80-81	34.13148399327213	35.0	34.0	36.0	30.0	38.5
82-83	33.943080588474146	35.0	34.0	36.0	30.0	37.0
84-85	33.73684176167653	35.0	33.0	35.0	29.5	37.0
86-87	33.558927916720066	35.0	33.0	35.0	29.0	36.5
88-89	33.39677382822282	35.0	33.0	35.0	29.0	36.0
90-91	33.265565093674006	35.0	33.0	35.0	29.0	36.0
92-93	33.1649952053549	35.0	33.0	35.0	29.0	35.5
94-95	33.130867001458114	35.0	33.0	35.0	29.0	35.0
96-97	33.17293888053824	35.0	33.0	35.0	29.0	35.0
98-99	33.237280683369235	34.0	33.0	35.0	29.5	35.0
100-101	31.752500664989228	33.0	30.0	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	19.0
20	84.0
21	476.0
22	2006.0
23	6749.0
24	17071.0
25	35328.0
26	62490.0
27	96678.0
28	137218.0
29	181372.0
30	231530.0
31	287166.0
32	360364.0
33	463475.0
34	636521.0
35	916744.0
36	1524269.0
37	2762439.0
38	2973700.0
39	1275958.0
40	60350.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.43294922734849	20.638697992995183	15.285410773878244	24.64294200577809
2	14.37333693120231	20.941534379724644	40.34296802410572	24.34216066496732
3	17.54259824772405	25.798659226401842	30.804730947259102	25.85401157861501
4	12.763296636496865	17.959103920218144	36.85800933160871	32.41959011167628
5	13.645019713665441	37.491477940217635	34.84270997470165	14.020792371415281
6	31.349353212751087	36.79314900778415	17.662154341806094	14.195343437658664
7	27.291734904786058	32.43277992893789	21.937051410117796	18.338433756158263
8	25.505065238476366	36.04501658492637	20.0990940000881	18.35082417650916
9	25.942606430679493	16.420722722138695	20.439951429561646	37.196719417620166
10-11	25.038964983003183	26.123293991160374	27.869593450176062	20.968147575660385
12-13	25.799612476839496	23.675183349586103	28.16832348484603	22.356880688728374
14-15	22.886928885308624	24.987151977755996	25.752606121630162	26.373313015305218
16-17	22.423767932373725	28.255873291328715	26.33737006535068	22.982988710946884
18-19	22.539039611835427	27.24020757961471	28.240481016887454	21.980271791662407
20-21	23.295557619057337	26.19540471554257	28.158580116458694	22.350457548941407
22-23	23.11518344412424	26.243233304897412	28.091999202209006	22.54958404876934
24-25	23.01207766373688	26.53159469358957	28.003353330945185	22.452974311728365
26-27	22.87245508403932	26.47468716625963	28.1975406747115	22.455317074989548
28-29	22.54325495197626	26.475895099908836	28.25400768043977	22.726842267675135
30-31	22.92799120605448	26.299282488895372	28.280070033359994	22.492656271690155
32-33	22.613065284496177	26.677602444303528	28.05352731236669	22.65580495883361
34-35	22.67115389752059	26.647962451801032	28.22939592307249	22.451487727605883
36-37	23.005011458771214	26.45069374878299	27.96270892277608	22.581585869669716
38-39	22.826827872484284	26.450936498886318	28.03270274143624	22.68953288719316
40-41	22.818668949389426	26.394939348069368	28.077438726336982	22.70895297620422
42-43	22.90068420271228	26.558724087785627	27.760487246056126	22.780104463445966
44-45	22.88680574086873	26.6627318411645	27.502689455835426	22.947772962131342
46-47	23.16183063192112	26.548863063350105	27.169900417927845	23.11940588680093
48-49	23.081557841731787	26.780241371374654	26.977762918272774	23.16043786862079
50-51	22.959329402196467	26.837725936889257	27.08975275453664	23.113191906377637
52-53	23.059066634039308	26.827675430222904	27.02980651774952	23.083451417988275
54-55	23.033733866941255	26.81858554945228	26.859375708952587	23.288304874653885
56-57	23.187367713794814	26.95390297256533	26.69352531101329	23.165204002626567
58-59	22.917881084147464	27.108768229952325	26.95612578513381	23.0172249007664
60-61	23.242658182178776	27.22186706088403	26.66210466030283	22.87337009663436
62-63	23.134679310433683	27.44085929009169	26.53626487186211	22.888196527612514
64-65	22.992380800703774	27.258254220357614	26.61872012448984	23.13064485444877
66-67	23.137883482539127	27.103386905147836	26.530012359595318	23.228717252717722
68-69	23.06133426817645	26.99501723257338	26.67563811331056	23.268010385939608
70-71	23.07060103295742	26.864517311885983	26.69962330989353	23.36525834526307
72-73	23.212298862310003	26.875566773972192	26.645943708625875	23.266190655091926
74-75	23.00587331134379	27.031662660312257	26.666820416061064	23.295643612282888
76-77	23.19405327991829	26.85678821459257	26.63497774899866	23.314180756490487
78-79	23.045938216280394	26.833256598301205	26.64209383005239	23.47871135536601
80-81	23.174014209444195	26.90510324365814	26.58308114513959	23.33780140175807
82-83	23.087392851220127	26.83864492729789	26.744499114573454	23.329463106908527
84-85	23.202800380001452	26.80921994241437	26.67017914046839	23.317800537115787
86-87	23.133470546907812	26.826838329730368	26.750781393438082	23.288909729923738
88-89	23.10028563575239	26.73082506651087	26.741898233887852	23.426991063848888
90-91	23.21824022973749	26.730408910029645	26.70002222443899	23.351328635793877
92-93	23.269069547625943	26.656819009107142	26.74538057126574	23.32873087200117
94-95	22.99245194702209	26.60510879334111	26.916767824102966	23.485671435533835
96-97	23.095505017041642	26.696622455789022	26.845414047954037	23.362458479215302
98-99	23.330537259896424	27.292654590383925	26.849141632751788	22.52766651696786
100-101	23.62776234761097	30.034431805114252	23.911417939490207	22.426387907784566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3428.0
1	2962.5
2	3659.0
3	5024.0
4	6613.5
5	9992.0
6	12884.0
7	16252.0
8	19385.0
9	19716.5
10	17669.5
11	15452.0
12	16602.5
13	22721.0
14	38038.0
15	58879.0
16	74892.0
17	82305.0
18	81168.5
19	74177.5
20	65136.0
21	56356.0
22	50432.5
23	51274.0
24	57640.0
25	68958.0
26	84618.0
27	100499.5
28	119327.5
29	140309.0
30	160558.5
31	183126.0
32	206879.0
33	233093.0
34	254796.0
35	270970.5
36	293448.5
37	318324.5
38	340772.0
39	359054.5
40	374683.5
41	393629.5
42	410701.0
43	430585.5
44	456452.5
45	484686.5
46	514158.0
47	533328.5
48	535592.0
49	532879.0
50	530108.0
51	525157.0
52	513941.0
53	496237.0
54	479356.5
55	456273.0
56	428377.0
57	395573.0
58	357051.0
59	313439.0
60	265202.0
61	220196.5
62	178908.5
63	139863.5
64	105719.5
65	79654.0
66	60952.5
67	47149.0
68	36158.5
69	26409.0
70	18904.5
71	13879.0
72	9834.0
73	6664.0
74	4519.0
75	2948.5
76	1852.5
77	1145.0
78	702.0
79	457.0
80	333.0
81	234.5
82	163.0
83	115.5
84	80.5
85	54.0
86	34.5
87	29.0
88	24.0
89	16.0
90	12.0
91	10.5
92	8.5
93	4.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.311163305216669E-6
2	0.022165872535012853
3	8.311163305216669E-6
4	8.311163305216669E-6
5	8.311163305216669E-6
6	8.311163305216669E-6
7	8.311163305216669E-6
8	0.0
9	0.0
10-11	4.404916551764834E-4
12-13	0.0017702777840111503
14-15	2.077790826304167E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007921510760592691
26-27	0.026733694214630545
28-29	0.3249306414628937
30-31	0.3108149769247659
32-33	0.31287831119271375
34-35	0.16480515582779057
36-37	0.025216502012497564
38-39	0.023655428134644052
40-41	0.008795453847125538
42-43	0.006141293091406525
44-45	0.0018189120665845103
46-47	0.0015535457179540384
48-49	0.001432226869347609
50-51	0.0015348426280658368
52-53	1.1732117669349848E-4
54-55	1.8203794298762768E-5
56-57	1.6080610353808806E-4
58-59	1.9490971016508437E-4
60-61	0.0019504189438940892
62-63	8.715756078576709E-4
64-65	9.576639282426249E-4
66-67	0.006502586983003149
68-69	0.009856862661635385
70-71	0.01377853919610011
72-73	0.013417240893824902
74-75	0.01039474381745877
76-77	0.005201495160815882
78-79	0.002218795070894909
80-81	0.002004660468242505
82-83	3.4006724936127137E-4
84-85	1.076125436193994E-5
86-87	0.0
88-89	6.523341122644894E-4
90-91	0.00233522372313363
92-93	0.014941638860360455
94-95	4.357710890982798E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	646.0
20-21	2360.0
22-23	3290.0
24-25	5479.0
26-27	9189.0
28-29	12945.0
30-31	26994.0
32-33	34748.0
34-35	49261.0
36-37	68360.0
38-39	81430.0
40-41	87075.0
42-43	102669.0
44-45	122908.0
46-47	121000.0
48-49	105827.0
50-51	94551.0
52-53	91883.0
54-55	101409.0
56-57	108546.0
58-59	109983.0
60-61	109468.0
62-63	110378.0
64-65	119829.0
66-67	125927.0
68-69	116880.0
70-71	110597.0
72-73	105766.0
74-75	109046.0
76-77	109339.0
78-79	117103.0
80-81	118311.0
82-83	116649.0
84-85	120053.0
86-87	124672.0
88-89	134790.0
90-91	144919.0
92-93	150381.0
94-95	171419.0
96-97	258856.0
98-99	1055960.0
100-101	7161114.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.41023579718166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.23693436253066	70.1474246231435
2	8.149022470362437	13.10529636716751
3	2.403248288115296	5.797372845795723
4	0.9736191865212667	3.1315579345934097
5	0.45864781396845977	1.843998943453288
6	0.25313942177986254	1.2213000356928583
7	0.15016362603303204	0.8452284804233139
8	0.09968464085926808	0.6412532381480891
9	0.06491985717111412	0.4698198920743777
>10	0.20657637046290067	2.5105514429841587
>50	0.0030723380995023595	0.16868538519419915
>100	9.511124156018053E-4	0.10720237961970291
>500	2.051168059924444E-5	0.010308431709885761
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.4933489915650003E-5	0.0	0.0	0.0
3	0.0	2.4933489915650003E-5	0.0	0.0	0.0
4	0.0	9.973395966260001E-5	0.0	8.311163305216667E-6	0.0
5	0.0	1.0804512296781669E-4	0.0	8.311163305216667E-6	0.0
6	0.0	1.0804512296781669E-4	0.0	1.6622326610433334E-5	2.4933489915650003E-5
7	0.0	1.0804512296781669E-4	0.0	1.6622326610433334E-5	2.4933489915650003E-5
8	0.0	1.4128977618868335E-4	0.0	2.4933489915650003E-5	2.4933489915650003E-5
9	0.0	2.1609024593563338E-4	0.0	3.324465322086667E-5	2.4933489915650003E-5
10-11	0.0	2.1609024593563338E-4	0.0	7.064488809434167E-5	2.4933489915650003E-5
12-13	0.0	2.1609024593563338E-4	0.0	9.973395966260001E-5	3.324465322086667E-5
14-15	0.0	3.075130422930167E-4	0.0	1.1635628627303335E-4	4.9866979831300006E-5
16-17	0.0	3.158242055982334E-4	0.0	1.7453442940955003E-4	6.648930644173334E-5
18-19	0.0	3.4491327716649177E-4	0.0	2.9920187898780005E-4	7.480046974695E-5
20-21	0.0	3.740023487347501E-4	0.0	4.695807267447418E-4	8.726721470477501E-5
22-23	0.0	4.072470019556167E-4	0.0	6.690486460699418E-4	1.2051186792564169E-4
24-25	0.0	4.654251450921334E-4	0.0	8.518942387847084E-4	1.3297861288346667E-4
26-27	0.0	4.737363083973501E-4	0.0	0.0010222730865416501	1.4128977618868335E-4
28-29	0.0	4.778918900499584E-4	0.0	0.0016372991711276837	1.4128977618868335E-4
30-31	0.0	5.734702680599501E-4	0.0	0.0025182824814806506	1.4128977618868335E-4
32-33	0.0	5.817814313651668E-4	0.0	0.0041888263058292	1.579121027991167E-4
34-35	0.0	6.648930644173334E-4	0.0	0.0064328403982377	1.703788477569417E-4
36-37	0.0	7.563158607747168E-4	0.0	0.0096741940872722	1.7453442940955003E-4
38-39	0.0	7.812493506903667E-4	0.0	0.014731536958496544	1.9946791932520002E-4
40-41	0.0	8.685165653951419E-4	0.0	0.020208593576634327	1.9946791932520002E-4
42-43	0.0	8.851388920055751E-4	0.0	0.025386448315784314	2.0362350097780836E-4
44-45	0.0	9.433170351420918E-4	0.0	0.031482686600160736	2.077790826304167E-4
46-47	0.0	9.890284333207834E-4	0.0	0.038455752613237526	2.2440140924085002E-4
48-49	0.0	9.931840149733917E-4	0.0	0.04537895164648301	2.327125725460667E-4
50-51	0.0	0.0011136958828990334	0.0	0.051786858554805054	2.3686815419867504E-4
52-53	0.0	0.0011261626278568585	0.0	0.057941274982318006	2.451793175038917E-4
54-55	0.0	0.001134473791162075	0.0	0.06463591702467003	2.534904808091084E-4
56-57	0.0	0.0011386293728146836	0.0	0.07146769326155814	2.61801644114325E-4
58-59	0.0	0.0011386293728146836	0.0	0.07783404435335409	2.6595722576693335E-4
60-61	0.0	0.0011386293728146836	0.0	0.0839718384542566	2.6595722576693335E-4
62-63	0.0	0.0011635628627303335	0.0	0.0898021195128661	2.6595722576693335E-4
64-65	0.0	0.0013297861288346668	0.0	0.09595653594037903	2.6595722576693335E-4
66-67	0.0	0.0013464084554451001	0.0	0.10238937633861674	2.6595722576693335E-4
68-69	0.0	0.0013838086903185751	0.0	0.10819887948896319	2.701128074195417E-4
70-71	0.0	0.0014045865985816168	0.0	0.11402084938426747	2.7842397072475836E-4
72-73	0.0	0.0014045865985816168	0.0	0.11848809966082142	3.075130422930167E-4
74-75	0.0	0.0014212089251920503	0.0	0.12230707919956849	3.075130422930167E-4
76-77	0.0	0.0014669203233707419	0.0	0.12557752196017125	3.075130422930167E-4
78-79	0.0	0.0014960093949390003	0.0	0.12847396237203923	3.075130422930167E-4
80-81	0.0	0.0014960093949390003	0.0	0.12976219268434785	3.2413536890345E-4
82-83	0.0	0.0015043205582442169	0.0	0.13031072946249214	3.4906885881910005E-4
84-85	0.0	0.001516787303202042	0.0	0.1306390204130482	3.532244404717084E-4
86-87	0.0	0.0015292540481598668	0.0	0.13077199902593167	3.573800221243167E-4
88-89	0.0	0.001604054517906817	0.0	0.1308010880974999	3.573800221243167E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	9275	0.0	33.860092	1
GGTATCA	27780	0.0	30.632849	1
GTATCAA	42250	0.0	28.45394	1
TATACAC	13195	0.0	26.706549	3
TCAACGC	46360	0.0	25.04319	4
ATCAACG	46490	0.0	24.96398	3
TTATACA	13360	0.0	24.739132	2
CAACGCA	47255	0.0	24.569387	5
AACGCAG	48365	0.0	24.128075	6
TATCAAC	52190	0.0	22.71114	2
GTACATG	39835	0.0	21.595758	1
TACACAT	16310	0.0	20.985456	5
ACGCAGA	55600	0.0	20.943539	7
TACATGG	40680	0.0	20.867012	2
CGCAGAG	57085	0.0	20.369143	8
ACATGGG	41885	0.0	19.829292	3
GAGTACT	32085	0.0	18.98775	12-13
AGAGTAC	51090	0.0	18.917852	10-11
ACACATC	17660	0.0	18.640717	6
ACATCTC	17525	0.0	18.59227	8
>>END_MODULE