FastQCFastQC Report
Fri 27 May 2016
SRR2139053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13371501
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218100.035.799171
GTATCAA351700.031.3556231
ATCAACG394550.027.1263083
TCAACGC394300.027.1004224
CAACGCA402650.026.5808145
AACGCAG416100.025.8237846
TATCAAC444100.024.5256422
ACGCAGA482250.022.2116767
CGCAGAG485000.022.120768
TACATGG357450.019.276082
GTACATG369350.019.1955871
GCAGAGT558000.019.1430999
GAGTACT319550.018.55457512-13
ACATGGG365500.018.4292643
AGAGTAC484150.018.3610610-11
CTTATAC89900.018.2394081
CAGAGTA519550.017.2408110-11
AGTACTT350650.016.87864312-13
GTACTTT360350.016.44870214-15
CATGGGG267450.016.3404874
Produced by FastQC (version 0.11.5)