##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13371501
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4459058859585	34.0	31.0	34.0	31.0	34.0
2	32.574995507235876	34.0	31.0	34.0	31.0	34.0
3	32.632392204884106	34.0	31.0	34.0	31.0	34.0
4	36.170565144481536	37.0	35.0	37.0	35.0	37.0
5	36.08425718249582	37.0	35.0	37.0	35.0	37.0
6	36.119581115089474	37.0	36.0	37.0	35.0	37.0
7	36.06816871194939	37.0	35.0	37.0	35.0	37.0
8	36.039266421922264	37.0	35.0	37.0	35.0	37.0
9	37.74521386940778	39.0	38.0	39.0	35.0	39.0
10-11	37.66682603546154	39.0	37.0	39.0	35.0	39.0
12-13	37.614496569981185	39.0	37.0	39.0	35.0	39.0
14-15	38.94463489925327	40.0	38.0	41.0	35.0	41.0
16-17	38.91509083385627	40.0	38.0	41.0	35.0	41.0
18-19	38.90822746825506	40.0	38.0	41.0	35.0	41.0
20-21	38.875379487054786	40.0	38.0	41.0	35.0	41.0
22-23	38.77102307647309	40.0	38.0	41.0	34.5	41.0
24-25	38.64513070668637	40.0	38.0	41.0	34.0	41.0
26-27	38.57611351528921	40.0	38.0	41.0	34.0	41.0
28-29	38.52234014117512	40.0	38.0	41.0	34.0	41.0
30-31	38.453448129427514	40.0	38.0	41.0	34.0	41.0
32-33	38.347705974514355	40.0	38.0	41.0	34.0	41.0
34-35	38.23704275665712	40.0	38.0	41.0	34.0	41.0
36-37	38.37799783909328	40.0	38.0	41.0	34.0	41.0
38-39	38.443586190602616	40.0	38.0	41.0	34.0	41.0
40-41	38.382212185561414	40.0	38.0	41.0	34.0	41.0
42-43	38.34728277768966	40.0	38.0	41.0	34.0	41.0
44-45	38.31484647828606	40.0	38.0	41.0	33.0	41.0
46-47	38.223738858799926	40.0	38.0	41.0	33.0	41.0
48-49	38.14915214332757	40.0	38.0	41.0	33.0	41.0
50-51	38.081028755759604	40.0	37.0	41.0	33.0	41.0
52-53	38.00871069763173	40.0	37.0	41.0	33.0	41.0
54-55	37.88950635842703	40.0	37.0	41.0	33.0	41.0
56-57	37.73655635571997	40.0	36.5	41.0	33.0	41.0
58-59	37.61362285636676	39.5	36.0	41.0	33.0	41.0
60-61	37.4689439416948	39.0	36.0	41.0	32.0	41.0
62-63	37.252281683673104	39.0	35.5	41.0	32.0	41.0
64-65	36.9259541733982	38.5	35.0	40.0	31.5	41.0
66-67	36.62939248068301	38.0	35.0	40.0	31.0	41.0
68-69	36.34901344255709	37.5	35.0	40.0	31.0	41.0
70-71	36.03772362187644	37.0	35.0	39.5	31.0	41.0
72-73	35.72958454463944	36.5	34.0	39.0	30.5	41.0
74-75	35.401435422058796	36.0	34.0	39.0	30.0	40.0
76-77	34.41788025193442	35.0	33.5	37.0	29.0	39.0
78-79	34.7421548317257	35.0	34.0	37.0	30.0	39.0
80-81	34.58669818785559	35.0	34.0	37.0	30.0	39.0
82-83	34.34471478467034	35.0	34.0	36.5	30.0	39.0
84-85	34.08424101811814	35.0	34.0	36.0	30.0	37.0
86-87	33.85264260093233	35.0	34.0	36.0	30.0	37.0
88-89	33.64124809902923	35.0	33.0	35.0	29.0	37.0
90-91	33.47031653233378	35.0	33.0	35.0	29.0	36.0
92-93	33.33939219597063	35.0	33.0	35.0	29.0	36.0
94-95	33.28097574184106	35.0	33.0	35.0	29.0	36.0
96-97	33.30229329181654	35.0	33.0	35.0	29.0	35.5
98-99	33.35145750402772	35.0	33.0	35.0	30.0	35.0
100-101	31.88250334671359	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	4.0
19	22.0
20	76.0
21	471.0
22	1986.0
23	7066.0
24	17394.0
25	35970.0
26	63611.0
27	99361.0
28	142670.0
29	189345.0
30	241826.0
31	302770.0
32	377781.0
33	484941.0
34	658311.0
35	922801.0
36	1482565.0
37	2768405.0
38	3673654.0
39	1809148.0
40	91317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.15610066185544	21.92475040197435	15.77213476423737	24.14701417193284
2	13.529189512942985	21.867571399280862	41.803238160223756	22.800000927552396
3	16.91974722357252	26.79987286392701	31.45331488613843	24.82706502636204
4	11.880409826870583	18.970751224619526	39.329536701192836	29.819302247317054
5	12.63585985117601	38.28337882810455	36.110959877351085	12.96980144336836
6	29.260135362524775	38.9898515499383	18.79267097932169	12.957342108215233
7	25.77187301349886	33.159914744045174	23.51997158134839	17.54824066110758
8	23.815157326017474	37.75452733391711	21.177495331301998	17.252820008763415
9	25.045804506165766	17.219570188866605	21.74345273578486	35.99117256918277
10-11	23.64030786199189	27.389763979153788	29.291188851228206	19.678739307626117
12-13	24.501916775460128	24.808585143783308	29.497749449576066	21.191748631180495
14-15	21.52747370021472	26.352864604464255	27.215393310113296	24.90426838520773
16-17	21.033264779568487	29.551953782298174	27.850779643271135	21.564001794862207
18-19	21.05620162285458	28.73003776689227	29.655479938675537	20.55828067157761
20-21	21.85718111842416	27.737601354866054	29.593896939921272	20.811320586788515
22-23	21.545232234333263	27.721017638593192	29.439818680734636	21.29393144633891
24-25	21.40277275321006	27.920329581211277	29.46475604509281	21.212141620485852
26-27	21.413253111460296	27.993412663696933	29.387619120211962	21.205715104630812
28-29	21.26377825390606	28.104904178504793	29.466083513278086	21.165234054311068
30-31	21.52814084351696	28.03824424254959	29.31564169838552	21.11797321554793
32-33	21.43229416634388	28.013492403821438	29.278888781733308	21.27532464810137
34-35	21.422525542526778	28.0559836803312	29.298830355015927	21.2226604221261
36-37	21.43440417607843	28.066662753644316	29.247495507913456	21.251437562363794
38-39	21.38649763391836	28.056312575389857	29.29120546523035	21.265984325461424
40-41	21.580879404109698	28.027756643671086	29.136892114121416	21.2544718380978
42-43	21.52744039395899	28.135444857486565	28.93661499629861	21.400499752255836
44-45	21.601268714364895	28.26079561560994	28.695392630273602	21.44254303975156
46-47	21.654097974625483	28.142773318960568	28.472332934885834	21.73079577152811
48-49	21.636390993165193	28.318162204041737	28.397479943480196	21.647966859312877
50-51	21.597677708559736	28.353891763353623	28.354413060695894	21.694017467390747
52-53	21.676820415816817	28.286311431576966	28.292957036849113	21.743911115757104
54-55	21.716806828277704	28.272525312374686	28.266725746433274	21.74394211291433
56-57	21.782281016042816	28.425019820524998	28.13452027174751	21.65817889168468
58-59	21.62715441668648	28.520200567774616	28.248463850534435	21.60418116500447
60-61	21.801946254665545	28.520994824992197	28.110398937762216	21.566659982580045
62-63	21.761635489279207	28.584892202706246	28.025562912484897	21.62790939552964
64-65	21.692147943705045	28.499579607384806	28.09316023307007	21.71511221584008
66-67	21.791506483870513	28.506649471960934	27.975546905466427	21.726297138702126
68-69	21.748893516461436	28.439700082348846	28.07464404997534	21.736762351214377
70-71	21.708096892972247	28.24381432681859	28.15751865686368	21.890570123345483
72-73	21.780494505555716	28.266053249062683	28.122918513689264	21.830533731692334
74-75	21.689300017372513	28.349641792090548	28.079377168806168	21.881681021730774
76-77	21.752682861532662	28.286238833424203	28.05217614443961	21.908902160603525
78-79	21.70517269667392	28.286439668977238	28.058061298994886	21.95032633535396
80-81	21.733670705357273	28.27908506435819	28.0899326938714	21.897311536413135
82-83	21.73379367230307	28.241781653213927	28.16747068874094	21.856953985742063
84-85	21.878939940216323	28.214386471107982	28.122765751971933	21.783907836703754
86-87	21.75451041809874	28.221137153774023	28.11316884309899	21.911183585028247
88-89	21.80450313777579	28.161137826640477	28.124508111225982	21.90985092435775
90-91	21.792573682137412	28.209223967730026	28.175812046680814	21.82239030345175
92-93	21.874704784839736	28.096128146870402	28.154416792831526	21.874750275458332
94-95	21.64388366371259	28.15782192434603	28.254513400314334	21.943781011627046
96-97	21.61591092383414	28.19530304815961	28.3407501148598	21.84803591314645
98-99	21.87973784190344	28.734972271697636	28.34384267620847	21.041447210190455
100-101	22.08158183873881	31.631158690692875	25.616761191639846	20.670498278928466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2246.0
1	2096.5
2	3097.5
3	4505.5
4	6190.5
5	9605.0
6	12453.5
7	15034.5
8	17148.5
9	17451.5
10	16263.0
11	14967.0
12	16478.5
13	22663.0
14	37983.0
15	59206.5
16	76245.0
17	85069.0
18	85805.5
19	80982.0
20	74444.0
21	69157.5
22	67734.5
23	74334.5
24	88928.5
25	110323.5
26	137878.5
27	166927.5
28	196539.0
29	227919.0
30	258284.0
31	289330.0
32	323732.0
33	356393.0
34	388124.5
35	419334.5
36	451396.5
37	477956.0
38	494176.0
39	505778.0
40	519140.5
41	532777.0
42	539575.0
43	548416.5
44	559456.0
45	570005.0
46	578657.0
47	574319.0
48	556552.5
49	536815.0
50	517270.0
51	491603.0
52	460032.5
53	428858.5
54	399802.0
55	367142.5
56	331844.0
57	295884.5
58	257827.5
59	220546.0
60	183157.5
61	147742.5
62	116192.5
63	88400.5
64	65475.0
65	48527.5
66	37078.0
67	28316.0
68	21207.5
69	15097.5
70	10563.0
71	7362.0
72	4995.0
73	3349.0
74	2218.5
75	1468.0
76	970.5
77	631.5
78	421.0
79	287.5
80	209.5
81	147.0
82	109.5
83	80.5
84	51.5
85	34.5
86	26.0
87	21.5
88	15.0
89	9.0
90	5.0
91	4.0
92	3.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.4785919695926425E-6
2	0.022316118437264446
3	7.4785919695926425E-6
4	7.4785919695926425E-6
5	7.4785919695926425E-6
6	7.4785919695926425E-6
7	7.4785919695926425E-6
8	0.0
9	0.0
10-11	4.113225583275954E-4
12-13	0.0016191151614168074
14-15	2.991436787837057E-5
16-17	7.4785919695926425E-6
18-19	7.4785919695926425E-6
20-21	3.739583343850912E-6
22-23	0.0
24-25	0.008324219092847928
26-27	0.02640885792618305
28-29	0.3283596534681834
30-31	0.314120929395353
32-33	0.3156277786269299
34-35	0.1691492423358608
36-37	0.02910441314214167
38-39	0.02749350056692969
40-41	0.010408348054852263
42-43	0.007889958596834007
44-45	0.0027158166355419275
46-47	0.001913078227894381
48-49	0.0018047212860396182
50-51	0.001856586064633415
52-53	2.2232814923376826E-4
54-55	2.852912860711094E-5
56-57	1.562530518174183E-4
58-59	3.4443159719197786E-4
60-61	0.002421125044867259
62-63	0.002012467659940656
64-65	0.0021303081761868178
66-67	0.007347538891584713
68-69	0.011017600595142346
70-71	0.015144646585673596
72-73	0.014543083193342047
74-75	0.01130121363327985
76-77	0.005643475184661108
78-79	0.0025357856420779525
80-81	0.0022074119678053153
82-83	3.016546606540608E-4
84-85	1.9084664198415237E-5
86-87	0.0
88-89	5.682791014625594E-4
90-91	0.0023073037152114917
92-93	0.015509894999678583
94-95	4.5771512006356166E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	479.0
20-21	1985.0
22-23	2996.0
24-25	5327.0
26-27	8525.0
28-29	12502.0
30-31	27434.0
32-33	35774.0
34-35	51787.0
36-37	72719.0
38-39	87172.0
40-41	92515.0
42-43	108845.0
44-45	128321.0
46-47	127832.0
48-49	112479.0
50-51	101264.0
52-53	99330.0
54-55	106992.0
56-57	110600.0
58-59	112194.0
60-61	110620.0
62-63	111940.0
64-65	121164.0
66-67	126501.0
68-69	123288.0
70-71	120028.0
72-73	116655.0
74-75	118503.0
76-77	119829.0
78-79	126894.0
80-81	129020.0
82-83	128182.0
84-85	131364.0
86-87	137744.0
88-89	148525.0
90-91	160060.0
92-93	166071.0
94-95	189769.0
96-97	285719.0
98-99	1158803.0
100-101	8133750.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.8054338139806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.78026498678653	74.40268621337799
2	7.664493338803001	12.846523784455002
3	1.9788621145074319	4.975181938930389
4	0.7414121057454328	2.485374526277314
5	0.34378646699413673	1.4405587002909674
6	0.17891789839640207	0.8996575255317708
7	0.09167022080611342	0.5377723835735808
8	0.059484977827031216	0.3988131497767495
9	0.03934718742840485	0.29677573006177255
>10	0.1190846344774509	1.4958281757681804
>50	0.002163729880800516	0.11796378731450258
>100	4.6801882442649745E-4	0.07134366108941585
>500	2.9548398937394647E-5	0.016801787214797116
>1k	1.4771123930528774E-5	0.014718636337635425
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	7.478591969592643E-6	0.0	0.0	0.0
2	0.0	2.243577590877793E-5	0.0	7.478591969592643E-6	0.0
3	0.0	2.243577590877793E-5	0.0	7.478591969592643E-6	0.0
4	0.0	6.73073277263338E-5	0.0	7.478591969592643E-6	0.0
5	0.0	6.73073277263338E-5	0.0	7.478591969592643E-6	0.0
6	0.0	6.73073277263338E-5	0.0	7.478591969592643E-6	0.0
7	0.0	7.478591969592643E-5	0.0	2.243577590877793E-5	0.0
8	0.0	8.226451166551907E-5	0.0	2.243577590877793E-5	1.4957183939185287E-5
9	0.0	1.6452902333103815E-4	0.0	2.9914367878370574E-5	1.4957183939185287E-5
10-11	7.478591969592643E-6	1.6452902333103815E-4	0.0	2.9914367878370574E-5	1.4957183939185287E-5
12-13	7.478591969592643E-6	1.6452902333103815E-4	0.0	4.861084780235218E-5	1.4957183939185287E-5
14-15	7.478591969592643E-6	2.729686068901315E-4	0.0	9.348239961990804E-5	1.869647992398161E-5
16-17	7.478591969592643E-6	2.767079028749278E-4	0.0	1.3087535946787124E-4	2.243577590877793E-5
18-19	7.478591969592643E-6	3.1784015870768735E-4	0.0	2.1687916711818665E-4	2.243577590877793E-5
20-21	7.478591969592643E-6	3.627117105252432E-4	0.0	3.3653663863166894E-4	2.243577590877793E-5
22-23	7.478591969592643E-6	4.3749763022116965E-4	0.0	5.833301736282261E-4	2.243577590877793E-5
24-25	7.478591969592643E-6	4.935870699931145E-4	0.0	7.590770849136533E-4	2.617507189357425E-5
26-27	7.478591969592643E-6	5.160228459018924E-4	0.0	9.011703323359135E-4	2.9914367878370574E-5
28-29	7.478591969592643E-6	5.160228459018924E-4	0.0	0.0016303330493711962	2.9914367878370574E-5
30-31	7.478591969592643E-6	5.608943977194482E-4	0.0	0.0026175071893574254	2.9914367878370574E-5
32-33	7.478591969592643E-6	5.683729896890409E-4	0.0	0.004580637581375494	3.7392959847963214E-5
34-35	7.478591969592643E-6	6.207231334761894E-4	0.0	0.007332759426185586	4.487155181755586E-5
36-37	7.478591969592643E-6	6.768125732481342E-4	0.0	0.011169277106586613	4.487155181755586E-5
38-39	7.478591969592643E-6	7.142055330960974E-4	0.0	0.016965185883020914	4.487155181755586E-5
40-41	7.478591969592643E-6	8.301237086247834E-4	0.0	0.023396774976870587	5.23501437871485E-5
42-43	7.478591969592643E-6	8.712559644575429E-4	0.0	0.0290767655777762	5.23501437871485E-5
44-45	7.478591969592643E-6	9.98392027940618E-4	0.0	0.03551209396761067	5.23501437871485E-5
46-47	7.478591969592643E-6	0.0010918744275605259	0.0	0.04316643284848874	5.6089439771944824E-5
48-49	7.478591969592643E-6	0.0010918744275605259	0.0	0.05067493918595975	5.982873575674115E-5
50-51	7.478591969592643E-6	0.0012190104910436007	0.0	0.05755150450200019	5.982873575674115E-5
52-53	7.478591969592643E-6	0.0012339676749827861	0.0	0.06448415925781256	6.356803174153747E-5
54-55	7.478591969592643E-6	0.0012489248589219715	0.0	0.07172343628437824	6.73073277263338E-5
56-57	7.478591969592643E-6	0.0012638820428611567	0.0	0.07809893593845597	6.73073277263338E-5
58-59	7.478591969592643E-6	0.0012713606348307494	0.0	0.08384997316307272	6.73073277263338E-5
60-61	7.478591969592643E-6	0.0012863178187699346	0.0	0.0895561388358719	6.73073277263338E-5
62-63	7.478591969592643E-6	0.00130127500270912	0.0	0.09509403618935525	6.73073277263338E-5
64-65	7.478591969592643E-6	0.0015031969858881214	0.0	0.10062819424685382	6.73073277263338E-5
66-67	7.478591969592643E-6	0.001521893465812103	0.0	0.10621470244813952	6.73073277263338E-5
68-69	7.478591969592643E-6	0.0015592864256600662	0.0	0.11159554937026142	6.73073277263338E-5
70-71	7.478591969592643E-6	0.0015854614975536405	0.0	0.1170100499562465	6.73073277263338E-5
72-73	7.478591969592643E-6	0.001592940089523233	0.0	0.12119432216323359	7.478591969592643E-5
74-75	7.478591969592643E-6	0.0016228544574016035	0.0	0.12480274278856204	7.478591969592643E-5
76-77	7.478591969592643E-6	0.0016714653052039559	0.0	0.12796618719169972	7.478591969592643E-5
78-79	7.478591969592643E-6	0.0016976403770975301	0.0	0.13096884186749116	7.852521568072275E-5
80-81	7.478591969592643E-6	0.0016976403770975301	0.0	0.1323635992698202	8.226451166551907E-5
82-83	7.478591969592643E-6	0.001708858265051919	0.0	0.13287962211572207	8.974310363511172E-5
84-85	7.478591969592643E-6	0.0017125975610367153	0.0	0.13322737664230816	9.348239961990804E-5
86-87	7.478591969592643E-6	0.001720076153006308	0.0	0.13336199129776083	9.722169560470437E-5
88-89	1.4957183939185287E-5	0.0017873834807326417	0.0	0.1333806877776848	1.04700287574297E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21810	0.0	35.79917	1
GTATCAA	35170	0.0	31.355623	1
ATCAACG	39455	0.0	27.126308	3
TCAACGC	39430	0.0	27.100422	4
CAACGCA	40265	0.0	26.580814	5
AACGCAG	41610	0.0	25.823784	6
TATCAAC	44410	0.0	24.525642	2
ACGCAGA	48225	0.0	22.211676	7
CGCAGAG	48500	0.0	22.12076	8
TACATGG	35745	0.0	19.27608	2
GTACATG	36935	0.0	19.195587	1
GCAGAGT	55800	0.0	19.143099	9
GAGTACT	31955	0.0	18.554575	12-13
ACATGGG	36550	0.0	18.429264	3
AGAGTAC	48415	0.0	18.36106	10-11
CTTATAC	8990	0.0	18.239408	1
CAGAGTA	51955	0.0	17.24081	10-11
AGTACTT	35065	0.0	16.878643	12-13
GTACTTT	36035	0.0	16.448702	14-15
CATGGGG	26745	0.0	16.340487	4
>>END_MODULE