FastQCFastQC Report
Fri 27 May 2016
SRR2139052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11744788
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198600.036.789511
CTTATAC101050.033.1186181
GTATCAA323650.031.6128861
ATCAACG395600.025.3082223
TCAACGC397800.025.1789554
CAACGCA402000.024.8950335
TATACAC158500.024.7349433
TATCAAC410650.024.5732442
AACGCAG413950.024.2280856
TTATACA158250.022.7621152
ACGCAGA467600.021.4491027
CGCAGAG474550.021.1798238
GTACATG324700.020.60061
TACATGG319550.020.2797152
ACATGGG323200.019.663873
ACATCTC173950.019.651678
ACACATC174900.019.3981446
GAGTACT300250.018.98442812-13
AGAGTAC440900.018.84725210-11
GCAGAGT535200.018.6524149
Produced by FastQC (version 0.11.5)