##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139052_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11744788
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45810064856003	34.0	31.0	34.0	31.0	34.0
2	32.59214027532894	34.0	31.0	34.0	31.0	34.0
3	32.65145100958825	34.0	31.0	34.0	31.0	34.0
4	36.18223027950781	37.0	35.0	37.0	35.0	37.0
5	36.09761623624028	37.0	35.0	37.0	35.0	37.0
6	36.13466109392524	37.0	36.0	37.0	35.0	37.0
7	36.084769771919255	37.0	36.0	37.0	35.0	37.0
8	36.0577313102629	37.0	35.0	37.0	35.0	37.0
9	37.76929357941582	39.0	38.0	39.0	35.0	39.0
10-11	37.693841046769	39.0	37.0	39.0	35.0	39.0
12-13	37.63782275167504	39.0	37.0	39.0	35.0	39.0
14-15	38.97023062485249	40.0	38.0	41.0	35.0	41.0
16-17	38.936525503908626	40.0	38.0	41.0	35.0	41.0
18-19	38.929939178127356	40.0	38.0	41.0	35.0	41.0
20-21	38.90103722565986	40.0	38.0	41.0	35.0	41.0
22-23	38.79732050620636	40.0	38.0	41.0	35.0	41.0
24-25	38.665759164202015	40.0	38.0	41.0	34.0	41.0
26-27	38.59567355747238	40.0	38.0	41.0	34.5	41.0
28-29	38.547305204559706	40.0	38.0	41.0	34.0	41.0
30-31	38.47513521833271	40.0	38.0	41.0	34.0	41.0
32-33	38.36014151560978	40.0	38.0	41.0	34.0	41.0
34-35	38.24315354515605	40.0	38.0	41.0	34.0	41.0
36-37	38.37854497521643	40.0	38.0	41.0	34.0	41.0
38-39	38.43715637158181	40.0	38.0	41.0	34.0	41.0
40-41	38.379867733776585	40.0	38.0	41.0	34.0	41.0
42-43	38.34308577761098	40.0	38.0	41.0	34.0	41.0
44-45	38.311623935223615	40.0	38.0	41.0	33.5	41.0
46-47	38.21621998209646	40.0	38.0	41.0	33.0	41.0
48-49	38.138604148245875	40.0	38.0	41.0	33.0	41.0
50-51	38.058252355784255	40.0	37.0	41.0	33.0	41.0
52-53	37.987642114464734	40.0	37.0	41.0	33.0	41.0
54-55	37.86497168965634	40.0	37.0	41.0	33.0	41.0
56-57	37.70051847187116	40.0	36.5	41.0	33.0	41.0
58-59	37.57418123863572	39.0	36.0	41.0	32.5	41.0
60-61	37.419804410491565	39.0	36.0	41.0	32.0	41.0
62-63	37.20199358465331	39.0	35.0	40.5	32.0	41.0
64-65	36.87942053460942	38.5	35.0	40.0	31.5	41.0
66-67	36.580889326509116	38.0	35.0	40.0	31.0	41.0
68-69	36.300102777323104	37.0	35.0	40.0	31.0	41.0
70-71	35.98706400370945	37.0	35.0	39.5	31.0	41.0
72-73	35.671738463954384	36.0	34.0	39.0	30.5	41.0
74-75	35.34610960133475	36.0	34.0	39.0	30.0	40.0
76-77	34.372629517634294	35.0	33.5	37.0	29.0	39.0
78-79	34.697698357305285	35.0	34.0	37.0	30.0	39.0
80-81	34.54709330762617	35.0	34.0	37.0	30.0	39.0
82-83	34.30812779415338	35.0	34.0	36.5	30.0	38.5
84-85	34.05285672276263	35.0	34.0	36.0	30.0	37.0
86-87	33.82897127800855	35.0	34.0	36.0	30.0	37.0
88-89	33.6218231245943	35.0	33.0	35.0	29.0	36.5
90-91	33.45469010079641	35.0	33.0	35.0	29.0	36.0
92-93	33.327870036706535	35.0	33.0	35.0	29.0	36.0
94-95	33.27077414898663	35.0	33.0	35.0	29.0	36.0
96-97	33.290920603456804	35.0	33.0	35.0	29.0	35.5
98-99	33.34213756533542	35.0	33.0	35.0	30.0	35.0
100-101	31.851077339499042	33.0	30.0	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	0.0
18	2.0
19	10.0
20	73.0
21	390.0
22	1925.0
23	6086.0
24	15914.0
25	32056.0
26	56107.0
27	87623.0
28	125500.0
29	166673.0
30	211477.0
31	264666.0
32	329998.0
33	423320.0
34	576152.0
35	815446.0
36	1327056.0
37	2494095.0
38	3221422.0
39	1516292.0
40	72503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.2749778029199	21.71411693425203	15.651776771109022	24.35912849171905
2	13.539214772025163	21.690535713297216	41.73155870772032	23.0386908069573
3	17.04159325821803	26.616861879499233	31.34931852324623	24.992226339036517
4	11.870635723692926	18.914134508004743	38.89141293993557	30.323816828366763
5	12.728837676763513	38.12602662559767	36.001032968836896	13.144102728801915
6	29.503529565625197	38.68162626690239	18.59624030676416	13.218603860708257
7	25.87422608224176	32.995376332037665	23.207247333881206	17.92315025183937
8	23.933390709138386	37.46474606438192	21.093654478905876	17.508208747573818
9	24.87446346413405	17.145418035642702	21.526782773771654	36.4533357264516
10-11	23.74293231691267	27.183088332239862	29.110337743859628	19.963641606987842
12-13	24.651632834456276	24.594678888818216	29.273438285601944	21.48024999112356
14-15	21.629255947608065	26.14884504692274	26.939906815659327	25.28199218980987
16-17	21.231106938669306	29.42820679266412	27.586449410581103	21.754236858085477
18-19	21.23007669444523	28.441300941319675	29.447640945072827	20.88098141916227
20-21	21.908111999333126	27.497520408389605	29.418112426346116	21.176255165931156
22-23	21.584482552707193	27.666662621806182	29.276678888531947	21.472175936954677
24-25	21.546454416284334	27.788995390193044	29.345634669796034	21.31891552372659
26-27	21.53417475702505	27.917443338088493	29.337472058258847	21.210909846627608
28-29	21.284274385583764	27.948312387201252	29.41676355217761	21.350649675037378
30-31	21.574950557460273	27.969383451433284	29.185160657588664	21.270505333517775
32-33	21.653693427027783	27.78304876644921	29.220065729250184	21.343192077272825
34-35	21.57016948348494	27.832270635591748	29.26757969567027	21.32998018525304
36-37	21.438774030176972	28.005005048915056	29.207402639362677	21.34881828154529
38-39	21.384665775201768	27.861617929053306	29.313913231597244	21.439803064147682
40-41	21.642644665814434	27.78665909331964	29.17587167921758	21.39482456164835
42-43	21.650172296204	27.84373505562776	28.917946634031054	21.588146014137177
44-45	21.590188749424478	28.043534243629644	28.71215773680906	21.654119270136817
46-47	21.844135756204206	27.922210706646013	28.35814651147993	21.87550702566985
48-49	21.770564052455434	28.03151174948349	28.33168108515775	21.866243112903323
50-51	21.55648025525089	28.149869325700198	28.42628451442575	21.86736590462316
52-53	21.74019443768746	28.16384567556541	28.317422816800086	21.778537069947042
54-55	21.763472610365245	28.08103338778944	28.21724538369156	21.93824861815376
56-57	21.921708131890867	28.257653895569945	27.978753668959722	21.841884303579462
58-59	21.695694800317618	28.44840321553999	28.23190052794641	21.624001456195973
60-61	21.922407130771347	28.54002643048058	27.980078526884412	21.557487911863657
62-63	21.741454093937893	28.74070966741749	27.86965864372303	21.64817759492158
64-65	21.70185577225596	28.59280097459433	27.946351743037525	21.758991510112192
66-67	21.773620059275895	28.478352005754097	27.859083839706695	21.88894409526331
68-69	21.811851546789953	28.283279626760223	27.97602046041485	21.928848366034973
70-71	21.781504722985826	28.113587904283115	28.043360778969557	22.061546593761502
72-73	21.889132964876588	28.1333914824851	28.05005381022924	21.927421742409074
74-75	21.76179712028467	28.302699517212954	27.98756999911388	21.947933363388497
76-77	21.78899507665814	28.21575206095211	27.94985556465976	22.04539729772999
78-79	21.81081490062251	28.13637857892018	27.968921379857335	22.08388514059997
80-81	21.847616562717725	28.17239533676301	27.971495286807148	22.00849281371212
82-83	21.84047972778555	28.143926877066285	28.10844269440629	21.907150700741873
84-85	21.946178706992274	28.10660082224155	28.038071691365708	21.90914877940047
86-87	21.824360457655725	28.139726461349657	28.07953507691337	21.956378004081248
88-89	21.853828675681225	28.02427546392502	28.042834348273477	22.079061512120283
90-91	21.907182396207848	28.074208670717432	28.062158669958688	21.956450263116036
92-93	21.939224639926078	27.968355311380826	28.143460672063387	21.948959376629713
94-95	21.735192097326355	28.000222520232093	28.215640919240837	22.04894446320072
96-97	21.661235284966033	28.13694964476482	28.24672509464295	21.955089975626198
98-99	21.93636020604567	28.618910713543904	28.29993160494462	21.14479747546581
100-101	22.092208821912674	31.46745773808066	25.63020296360469	20.810130476401973
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2288.0
1	2016.0
2	2857.5
3	4243.0
4	5766.0
5	8756.5
6	11242.5
7	13821.0
8	16008.0
9	16089.0
10	14696.0
11	13376.0
12	14860.5
13	20750.5
14	35216.5
15	55039.5
16	70371.0
17	77798.0
18	78028.5
19	73636.5
20	67366.5
21	61801.5
22	60352.0
23	65206.5
24	75930.0
25	92800.0
26	114060.0
27	137177.5
28	163279.5
29	191711.0
30	219181.0
31	245490.0
32	273175.5
33	301367.5
34	327977.0
35	351819.5
36	378362.0
37	402001.5
38	418918.5
39	433172.0
40	444921.5
41	456071.5
42	463216.5
43	468434.5
44	476157.0
45	487045.0
46	500358.0
47	510176.0
48	503887.0
49	485731.5
50	466274.5
51	443590.5
52	417005.5
53	390127.0
54	363755.5
55	334186.5
56	303299.0
57	271570.5
58	237053.5
59	201534.0
60	166403.0
61	134841.5
62	106217.5
63	80663.5
64	59884.5
65	43904.5
66	32849.5
67	25083.5
68	18958.5
69	13800.5
70	9873.0
71	6985.5
72	4864.0
73	3280.0
74	2161.0
75	1360.0
76	872.5
77	574.5
78	373.0
79	248.0
80	180.5
81	133.0
82	99.0
83	67.5
84	39.5
85	23.0
86	14.0
87	10.0
88	8.5
89	6.0
90	4.5
91	3.5
92	2.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.022077878289501692
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	3.7889147083795805E-4
12-13	0.001796541580827172
14-15	2.980045276253603E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008044517482474003
26-27	0.026408694463035287
28-29	0.3251317146662898
30-31	0.3108314740864793
32-33	0.3121838752246543
34-35	0.16820617939681712
36-37	0.030348051441864136
38-39	0.028853917209533335
40-41	0.011311640043903467
42-43	0.009187583937714154
44-45	0.003260434420104937
46-47	0.0021835466005087844
48-49	0.002109592947039008
50-51	0.002063811314615461
52-53	3.3516977290227686E-4
54-55	8.330152834702418E-5
56-57	3.687969577145344E-4
58-59	6.689261295453648E-4
60-61	0.003247361304984709
62-63	0.0026253424644056113
64-65	0.0027195212324075215
66-67	0.006037594414597884
68-69	0.00959101483990217
70-71	0.013794052689674987
72-73	0.013219948370865237
74-75	0.010086642934270857
76-77	0.005203769118882635
78-79	0.002008903355312127
80-81	0.0018365013509367264
82-83	2.40226325228542E-4
84-85	1.0802194746719858E-5
86-87	0.0
88-89	7.09110552661929E-4
90-91	0.0022874244988334417
92-93	0.014220399291229268
94-95	3.1937822749845377E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	246.0
20-21	880.0
22-23	1828.0
24-25	3858.0
26-27	7307.0
28-29	10433.0
30-31	23418.0
32-33	30876.0
34-35	44678.0
36-37	62019.0
38-39	74179.0
40-41	78471.0
42-43	93021.0
44-45	110924.0
46-47	109327.0
48-49	96327.0
50-51	86247.0
52-53	84061.0
54-55	92128.0
56-57	96469.0
58-59	97234.0
60-61	98655.0
62-63	101416.0
64-65	107964.0
66-67	117963.0
68-69	106594.0
70-71	101368.0
72-73	97217.0
74-75	99714.0
76-77	100730.0
78-79	107469.0
80-81	108754.0
82-83	107754.0
84-85	111999.0
86-87	117035.0
88-89	126301.0
90-91	136785.0
92-93	142447.0
94-95	164127.0
96-97	249247.0
98-99	1029889.0
100-101	7207429.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.1244439818348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.30626409236004	73.40409102790493
2	7.898836895416841	13.131728500694544
3	2.0972712803323335	5.2300352717008805
4	0.797318467125338	2.651066170249703
5	0.3611956366838079	1.5012093234003174
6	0.18544088373434187	0.9248802211150344
7	0.11498163819990827	0.669044932044151
8	0.06974911061461596	0.4638284830453956
9	0.046829416717181	0.35034023039483797
>10	0.11984641118191834	1.4939174000424489
>50	0.001861715380091319	0.10518247416173118
>100	3.873142720373236E-4	0.06386336363566725
>500	1.713798161130447E-5	0.010812601610396403
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.514415075010294E-6	0.0
2	0.0	1.7028830150020588E-5	0.0	1.7028830150020588E-5	0.0
3	0.0	2.5543245225030883E-5	0.0	1.7028830150020588E-5	0.0
4	0.0	5.108649045006177E-5	0.0	2.5543245225030883E-5	1.7028830150020588E-5
5	0.0	5.108649045006177E-5	0.0	2.5543245225030883E-5	1.7028830150020588E-5
6	0.0	5.108649045006177E-5	0.0	2.5543245225030883E-5	2.5543245225030883E-5
7	0.0	5.108649045006177E-5	0.0	2.5543245225030883E-5	2.5543245225030883E-5
8	0.0	5.108649045006177E-5	0.0	3.4057660300041176E-5	2.5543245225030883E-5
9	0.0	7.662973567509265E-5	0.0	5.108649045006177E-5	3.4057660300041176E-5
10-11	1.2771622612515442E-5	7.662973567509265E-5	0.0	5.108649045006177E-5	3.4057660300041176E-5
12-13	3.4057660300041176E-5	8.94013582876081E-5	0.0	5.5343697987566916E-5	3.4057660300041176E-5
14-15	3.4057660300041176E-5	1.5325947135018527E-4	0.0	5.960090552507206E-5	4.682928291255662E-5
16-17	4.257207537505147E-5	1.6177388642519557E-4	0.0	5.960090552507206E-5	5.960090552507206E-5
18-19	4.257207537505147E-5	1.7454550903771103E-4	0.0	1.2345901858764927E-4	7.662973567509265E-5
20-21	4.257207537505147E-5	1.8731713165022647E-4	0.0	1.6603109396270074E-4	8.514415075010294E-5
22-23	7.23725281375875E-5	1.9157433918773163E-4	0.0	1.9583154672523674E-4	1.1494460351263897E-4
24-25	8.514415075010294E-5	2.2137479195026766E-4	0.0	2.171175844127625E-4	1.277162261251544E-4
26-27	8.514415075010294E-5	2.3840362210028824E-4	0.0	2.6394686732531913E-4	1.277162261251544E-4
28-29	1.0217298090012353E-4	2.3840362210028824E-4	0.0	6.68381583388308E-4	1.277162261251544E-4
30-31	1.1068739597513382E-4	2.5968965978781397E-4	0.0	0.001715654637614574	1.3197343366265957E-4
32-33	1.277162261251544E-4	2.724612824003294E-4	0.0	0.0034398236903041587	1.44745056275175E-4
34-35	1.277162261251544E-4	3.0226173516286544E-4	0.0	0.00608780677863236	1.7028830150020587E-4
36-37	1.362306412001647E-4	3.448338105379169E-4	0.0	0.009872464279474436	1.7028830150020587E-4
38-39	1.362306412001647E-4	3.5760543315043234E-4	0.0	0.01618590305759457	1.8731713165022647E-4
40-41	1.4048784873766984E-4	4.001775085254838E-4	0.0	0.02274625987289	1.8731713165022647E-4
42-43	1.6603109396270074E-4	4.34235168825525E-4	0.0	0.028612691859572093	1.8731713165022647E-4
44-45	1.7880271657521617E-4	4.8532165927558674E-4	0.0	0.03558174059846801	1.9583154672523677E-4
46-47	1.8731713165022647E-4	5.236365271131332E-4	0.0	0.04318511326045221	2.0434596180024707E-4
48-49	1.9157433918773163E-4	5.278937346506383E-4	0.0	0.050183962452110674	2.2988920702527794E-4
50-51	2.0434596180024707E-4	6.08780677863236E-4	0.0	0.05687629270106877	2.2988920702527794E-4
52-53	2.1286037687525734E-4	6.215523004757514E-4	0.0	0.06323656076210145	2.4266082963779337E-4
54-55	2.171175844127625E-4	6.38581130625772E-4	0.0	0.07044827033063517	2.5968965978781397E-4
56-57	2.341464145627831E-4	6.556099607757926E-4	0.0	0.07694476903286802	2.6394686732531913E-4
58-59	2.937473200878551E-4	6.598671683132977E-4	0.0	0.08253448252961229	2.6394686732531913E-4
60-61	3.1503335777538087E-4	6.683815833883082E-4	0.0	0.08797519376254387	2.724612824003294E-4
62-63	3.1503335777538087E-4	6.811532060008235E-4	0.0	0.09328393156181278	3.0226173516286544E-4
64-65	3.1503335777538087E-4	7.492685266009059E-4	0.0	0.09887790226609455	3.065189427003706E-4
66-67	3.19290565312886E-4	7.662973567509264E-4	0.0	0.10465919010202654	3.1077615023787576E-4
68-69	3.278049803878963E-4	7.833261869009471E-4	0.0	0.11023613197615828	3.19290565312886E-4
70-71	3.3206218792540147E-4	7.918406019759573E-4	0.0	0.11521706479503929	3.448338105379169E-4
72-73	3.533482256129272E-4	7.918406019759573E-4	0.0	0.11938061376671932	4.001775085254838E-4
74-75	3.661198482254426E-4	8.046122245884727E-4	0.0	0.12308864153188631	4.001775085254838E-4
76-77	3.661198482254426E-4	8.258982622759985E-4	0.0	0.12632837646792772	4.0443471606298897E-4
78-79	3.661198482254426E-4	8.344126773510088E-4	0.0	0.1297852289883819	4.0869192360049413E-4
80-81	3.661198482254426E-4	8.344126773510088E-4	0.0	0.13119436468329612	4.299779612880199E-4
82-83	3.661198482254426E-4	8.344126773510088E-4	0.0	0.1316754291350342	4.7680724420057647E-4
84-85	3.661198482254426E-4	8.599559225760397E-4	0.0	0.13197343366265957	4.7680724420057647E-4
86-87	3.661198482254426E-4	8.6847033765105E-4	0.0	0.13204580619079714	4.8106445173808163E-4
88-89	3.661198482254426E-4	8.940135828760809E-4	0.0	0.13205857781340966	4.8532165927558674E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19860	0.0	36.78951	1
CTTATAC	10105	0.0	33.118618	1
GTATCAA	32365	0.0	31.612886	1
ATCAACG	39560	0.0	25.308222	3
TCAACGC	39780	0.0	25.178955	4
CAACGCA	40200	0.0	24.895033	5
TATACAC	15850	0.0	24.734943	3
TATCAAC	41065	0.0	24.573244	2
AACGCAG	41395	0.0	24.228085	6
TTATACA	15825	0.0	22.762115	2
ACGCAGA	46760	0.0	21.449102	7
CGCAGAG	47455	0.0	21.179823	8
GTACATG	32470	0.0	20.6006	1
TACATGG	31955	0.0	20.279715	2
ACATGGG	32320	0.0	19.66387	3
ACATCTC	17395	0.0	19.65167	8
ACACATC	17490	0.0	19.398144	6
GAGTACT	30025	0.0	18.984428	12-13
AGAGTAC	44090	0.0	18.847252	10-11
GCAGAGT	53520	0.0	18.652414	9
>>END_MODULE