FastQCFastQC Report
Fri 27 May 2016
SRR2139051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11665664
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144450.048.2416951
GTATCAA269800.037.0821461
TCAACGC331650.029.4546994
ATCAACG332550.029.4005153
CAACGCA341250.028.614015
TATCAAC347150.028.3426462
AACGCAG350950.027.8715276
ACGCAGA407750.023.947867
CGCAGAG415850.023.4813988
CTTATAC81400.020.7630021
GCAGAGT477200.020.3024319
GTACATG325600.020.2152351
TACATGG323450.020.0213892
ACATGGG327200.019.150983
AGAGTAC435750.018.8430810-11
GAGTACT300600.018.6640312-13
GTGGTAT76450.018.5524161
TATACAC129250.017.375733
CAGAGTA472550.017.3756710-11
CATGGGG249200.017.2405244
Produced by FastQC (version 0.11.5)