FastQCFastQC Report
Fri 27 May 2016
SRR2139050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10105052
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207150.051.9116061
GTATCAA391350.037.8374251
TCAACGC488600.029.951734
ATCAACG485950.029.945943
TATCAAC494700.029.6161582
CAACGCA498050.029.346565
AACGCAG509600.028.7579926
GTGGTAT86150.027.738411
TGGTATC88100.025.6746962
ACGCAGA581100.025.2195367
CGCAGAG588350.024.9017668
GCAGAGT653300.022.3567229
GTACATG439000.020.741331
GAGTACT407350.020.39989312-13
TACATGG442500.020.2141482
CTTATAC67550.019.5789091
CAGAGTA647200.019.44898210-11
ACATGGG450950.019.387683
AGAGTAC618350.018.75773210-11
GTACTTT448500.018.42340314-15
Produced by FastQC (version 0.11.5)