##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139050_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10105052
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71090717791457	33.0	33.0	33.0	33.0	33.0
2	32.724411908023825	33.0	33.0	33.0	33.0	33.0
3	32.617754168904824	33.0	33.0	33.0	33.0	33.0
4	36.60136731607121	37.0	37.0	37.0	37.0	37.0
5	36.65872041034524	37.0	37.0	37.0	37.0	37.0
6	36.67729448596603	37.0	37.0	37.0	37.0	37.0
7	36.659096261948974	37.0	37.0	37.0	37.0	37.0
8	36.66383201194808	37.0	37.0	37.0	37.0	37.0
9	36.656033041690435	37.0	37.0	37.0	37.0	37.0
10-11	36.627524826195845	37.0	37.0	37.0	37.0	37.0
12-13	36.59914050912356	37.0	37.0	37.0	37.0	37.0
14-15	38.70158659252817	40.0	37.0	40.0	37.0	40.0
16-17	38.663277982142006	40.0	37.0	40.0	37.0	40.0
18-19	38.710430980464025	40.0	37.0	40.0	37.0	40.0
20-21	38.74992104507689	40.0	37.0	40.0	37.0	40.0
22-23	38.78574634997307	40.0	37.0	40.0	37.0	40.0
24-25	38.80340683331785	40.0	37.0	40.0	37.0	40.0
26-27	38.514547465453504	40.0	37.0	40.0	37.0	40.0
28-29	38.479510104384595	40.0	37.0	40.0	37.0	40.0
30-31	38.472523994572335	40.0	37.0	40.0	37.0	40.0
32-33	38.45826284544205	40.0	37.0	40.0	37.0	40.0
34-35	38.285619793202805	40.0	37.0	40.0	37.0	40.0
36-37	38.34696716147654	40.0	37.0	40.0	37.0	40.0
38-39	38.28092882817994	40.0	37.0	40.0	37.0	40.0
40-41	38.29173735851427	40.0	37.0	40.0	37.0	40.0
42-43	38.24768038665788	40.0	37.0	40.0	37.0	40.0
44-45	38.17512690176244	40.0	37.0	40.0	37.0	40.0
46-47	38.093140429406006	40.0	37.0	40.0	35.0	40.0
48-49	37.86507412125532	40.0	37.0	40.0	33.0	40.0
50-51	37.96518108886764	40.0	37.0	40.0	33.0	40.0
52-53	37.943573024074226	40.0	37.0	40.0	33.0	40.0
54-55	33.408234212238604	37.0	33.0	40.0	10.5	40.0
56-57	36.60968384119066	37.0	35.0	40.0	30.0	40.0
58-59	37.409983728437176	38.5	37.0	40.0	33.0	40.0
60-61	37.45203711910056	38.5	37.0	40.0	33.0	40.0
62-63	37.22729854311666	37.0	37.0	40.0	33.0	40.0
64-65	32.34701253138858	37.0	33.0	40.0	8.5	40.0
66-67	35.41293110866571	37.0	33.0	40.0	30.0	40.0
68-69	36.28076507029094	37.0	37.0	40.0	33.0	40.0
70-71	36.20877583130485	37.0	37.0	38.5	33.0	40.0
72-73	35.5694652259997	37.0	37.0	37.0	33.0	40.0
74-75	35.6363680895673	37.0	35.0	37.0	33.0	40.0
76-77	33.82272067387021	35.0	33.0	37.0	30.0	37.0
78-79	35.3483135523265	37.0	33.0	37.0	33.0	37.0
80-81	35.5517356422768	37.0	33.0	37.0	33.0	37.0
82-83	35.48936731440492	37.0	33.0	37.0	33.0	37.0
84-85	35.37111079856395	37.0	33.0	37.0	33.0	37.0
86-87	35.2698319198998	37.0	33.0	37.0	33.0	37.0
88-89	35.18547433175628	37.0	33.0	37.0	33.0	37.0
90-91	35.091349496214725	37.0	33.0	37.0	33.0	37.0
92-93	35.02609464705134	37.0	33.0	37.0	33.0	37.0
94-95	34.977991456816994	37.0	33.0	37.0	33.0	37.0
96-97	34.94469563976295	37.0	33.0	37.0	33.0	37.0
98-99	34.90931960392466	37.0	33.0	37.0	33.0	37.0
100	34.86715545792318	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	26.0
20	66.0
21	308.0
22	999.0
23	2787.0
24	6822.0
25	13893.0
26	24950.0
27	40562.0
28	61188.0
29	88849.0
30	123333.0
31	170317.0
32	233039.0
33	323831.0
34	470304.0
35	772597.0
36	1614376.0
37	2647273.0
38	3370471.0
39	139058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.357688249173606	20.228339273102137	14.581183212237129	22.83278926548713
2	14.641450102527934	20.98999797230118	42.19086078833249	22.177691136838398
3	18.40067112984773	26.604920794188793	30.42828071666486	24.566127359298612
4	12.334176184871508	18.033885482248454	38.86569958459177	30.766238748288266
5	13.037583989069814	37.98996139714579	35.122648835569976	13.849805778214419
6	29.402520640170877	37.96331775432724	18.61825154388122	14.015910061620662
7	26.16137947632531	33.40513240307917	22.689512137097363	17.743975983498157
8	24.46683104649041	36.50194971782431	20.914637549613797	18.11658168607148
9	25.89630414568871	16.807167345600995	21.399790916464358	35.896737592245934
10-11	24.129514622982644	27.06917292459257	28.29641549593213	20.504896956492654
12-13	25.32893942554675	24.850648962518946	28.765987547614795	21.05442406431951
14-15	22.374733944961392	25.892370469741273	27.138336348986623	24.594559236310708
16-17	20.97605237459441	29.341491760754916	27.635612364983373	22.046843499667297
18-19	20.992286828410187	27.8592777157406	30.47499409206405	20.673441363785162
20-21	22.19958171357895	26.7801431171537	30.373910969130165	20.646364200137192
22-23	21.909626407176017	26.78588359460346	30.286209501843903	21.018280496376622
24-25	21.50647470270379	27.09982548775645	30.33995610688166	21.053743702658103
26-27	21.475764983675088	27.140116136817273	30.247113113288954	21.13700576621869
28-29	21.159448224181954	27.245214424428678	30.422328398878257	21.173008952511115
30-31	21.45770794844049	27.355126917956706	30.181013454986918	21.006151678615886
32-33	21.305460786541765	27.265456898627615	30.292736830636922	21.136345484193697
34-35	21.360781090408253	27.232098695857836	30.277230380063546	21.129889833670365
36-37	21.395751814720512	27.495876268624585	29.9484311993579	21.159940717297005
38-39	21.386312068018125	27.42284224950583	30.010840559319252	21.180005123156796
40-41	21.443444906602565	27.353802482557644	29.90321810250935	21.29953450833045
42-43	21.450440376071327	27.519179020994933	29.57922468298961	21.451155919944128
44-45	21.660586415408197	27.66851062690237	29.18810801427167	21.482794943417765
46-47	21.78944197684834	27.868588837423143	28.56625121214603	21.775717973582484
48-49	21.769421665797104	28.177483398594294	28.15998145693917	21.89311347866943
50-51	21.783164446054727	28.1544513112981	28.125085103260066	21.937299139387108
52-53	21.93958328300902	28.084517735343713	28.011110733413112	21.96478824823415
54-55	22.975690811728505	28.053634476653112	27.24305813361203	21.72761657800636
56-57	22.031419225202747	28.175286496893285	27.777152139399213	22.01614213850475
58-59	21.829050168908495	28.30794348316492	27.95481591564132	21.908190432285267
60-61	22.038129451606203	28.371687614109515	27.759711779306233	21.83047115497805
62-63	22.002765947886346	28.467553654585686	27.627198567891142	21.902481829636823
64-65	22.442284282502076	28.24034084676977	27.38967455812532	21.92770031260283
66-67	22.05765358485531	28.233298262930468	27.593301517876583	22.11574663433764
68-69	22.01997367962235	28.20160467869345	27.69222314591412	22.086198495770084
70-71	22.01021701077627	28.058350755504215	27.76680437764337	22.164627856076144
72-73	22.093938678300542	28.104174010710686	27.618283877427384	22.18360343356139
74-75	21.968467741466227	28.157404653980304	27.71161896443533	22.16250864011814
76-77	22.103355695392704	28.07085271939676	27.59183992806112	22.233951657149422
78-79	22.03258703478387	28.06877412764935	27.643726936345566	22.254911901221217
80-81	22.05114763051688	28.024182721098224	27.685686941877773	22.238982706507123
82-83	22.02893103802331	28.01779870664167	27.808902455391394	22.144367799943623
84-85	22.211002827300494	27.967544912626778	27.661533501581008	22.159918758491713
86-87	22.086345630313726	27.949940143082753	27.767293324238416	22.196420902365105
88-89	22.143727242987048	27.83234215376759	27.75251141673959	22.271419186505774
90-91	22.166933265308433	27.794640935852915	27.751031024855155	22.287394773983497
92-93	22.2718164440968	27.763997129989836	27.748485779654974	22.21570064625839
94-95	22.087816820012357	27.742998979795164	27.896752269198455	22.27243193099402
96-97	22.166063292759052	27.881985664360577	27.918602833452848	22.03334820942752
98-99	23.34326057988675	29.773010383919875	28.036506817172913	18.847222219020463
100	22.279905140466873	30.70560024949145	21.921341640918012	25.09315296912366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2125.0
1	2159.0
2	3666.0
3	5454.5
4	8111.0
5	14625.0
6	19750.5
7	22754.0
8	24558.5
9	23611.5
10	20994.0
11	18247.0
12	19567.0
13	27671.5
14	49113.0
15	79207.0
16	103745.5
17	117235.0
18	117607.5
19	107205.5
20	91411.5
21	74710.0
22	62636.0
23	59650.0
24	65247.5
25	78035.5
26	95491.5
27	113816.0
28	133437.0
29	155996.0
30	178370.0
31	200822.5
32	224611.5
33	247640.5
34	269131.5
35	287841.0
36	307459.5
37	327826.0
38	343658.5
39	355417.0
40	366548.0
41	378226.5
42	387826.0
43	396743.5
44	405319.0
45	409753.0
46	413160.5
47	415792.5
48	412402.0
49	405777.0
50	396607.0
51	382579.0
52	366244.0
53	349012.5
54	331129.5
55	310302.5
56	286797.0
57	260433.0
58	230693.5
59	198231.5
60	164265.0
61	132533.0
62	104052.0
63	79292.5
64	58928.5
65	43867.5
66	33028.5
67	24799.0
68	18560.5
69	13153.0
70	8993.0
71	6440.0
72	4350.5
73	2858.5
74	1948.0
75	1273.5
76	838.0
77	536.5
78	359.5
79	262.5
80	184.0
81	123.5
82	83.0
83	56.0
84	40.5
85	26.0
86	17.0
87	13.0
88	9.5
89	7.5
90	5.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	9.896040119338327E-6
2	9.896040119338327E-6
3	0.0029787080759208365
4	0.01242942638988894
5	0.016368050357385592
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0017026743519975313
26-27	0.034823860941073245
28-29	0.08135764580233254
30-31	0.07935337940564632
32-33	0.03475905177625325
34-35	7.394061090132406E-4
36-37	5.021744656537272E-6
38-39	1.0114819895264074E-5
40-41	0.0
42-43	5.147797381047787E-6
44-45	5.210006233772459E-6
46-47	0.0
48-49	5.355551015238417E-6
50-51	1.0828246629126218E-5
52-53	0.0
54-55	1.2573366893503106
56-57	7.24032020705979E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.228101994479588
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.009107451567808704
74-75	0.031105465591568706
76-77	0.004759633848609602
78-79	0.0
80-81	0.0
82-83	0.003890255320597095
84-85	0.03860140614683786
86-87	0.04138666187825745
88-89	0.013635725094474433
90-91	1.3482734616772818E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	169.0
20-21	529.0
22-23	1261.0
24-25	4551.0
26-27	7339.0
28-29	11868.0
30-31	26647.0
32-33	34195.0
34-35	46978.0
36-37	65184.0
38-39	80761.0
40-41	87732.0
42-43	108332.0
44-45	134121.0
46-47	131490.0
48-49	106178.0
50-51	88199.0
52-53	86969.0
54-55	82919.0
56-57	88305.0
58-59	88685.0
60-61	88206.0
62-63	93459.0
64-65	87996.0
66-67	95837.0
68-69	93037.0
70-71	91165.0
72-73	85653.0
74-75	87405.0
76-77	88098.0
78-79	93035.0
80-81	94311.0
82-83	90955.0
84-85	91841.0
86-87	95925.0
88-89	101869.0
90-91	106033.0
92-93	110574.0
94-95	125439.0
96-97	315691.0
98-99	571870.0
100-101	6214241.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.1973482461985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.11652587965642	75.03375163980793
2	7.472011390395189	12.582470902733311
3	1.876728271168052	4.740466314330677
4	0.7040664085304105	2.3712209834994096
5	0.33350123402593884	1.4039959770909458
6	0.17588670842535792	0.8885516664700461
7	0.09354501973341259	0.5513369822234155
8	0.0639286702547239	0.4306099609882704
9	0.04034406447926259	0.3057176921964803
>10	0.1212437830545496	1.5401920238884221
>50	0.0018169572728168055	0.0999717271398316
>100	4.0161300379586964E-4	0.05171412963123463
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.896040119338327E-6
2	0.0	9.896040119338327E-6	0.0	0.0	9.896040119338327E-6
3	0.0	1.9792080238676654E-5	0.0	0.0	9.896040119338327E-6
4	0.0	2.968812035801498E-5	0.0	0.0	9.896040119338327E-6
5	0.0	3.958416047735331E-5	0.0	0.0	9.896040119338327E-6
6	0.0	3.958416047735331E-5	0.0	0.0	9.896040119338327E-6
7	0.0	3.958416047735331E-5	0.0	0.0	9.896040119338327E-6
8	0.0	3.958416047735331E-5	0.0	0.0	9.896040119338327E-6
9	0.0	7.916832095470662E-5	0.0	0.0	9.896040119338327E-6
10-11	0.0	8.411634101437577E-5	0.0	0.0	9.896040119338327E-6
12-13	0.0	8.906436107404494E-5	0.0	4.9480200596691636E-6	9.896040119338327E-6
14-15	0.0	1.2864852155139824E-4	0.0	9.896040119338327E-6	1.9792080238676654E-5
16-17	0.0	1.2864852155139824E-4	0.0	2.4740100298345818E-5	2.968812035801498E-5
18-19	0.0	1.484406017900749E-4	0.0	5.44282206563608E-5	2.968812035801498E-5
20-21	0.0	1.731807020884207E-4	0.0	5.937624071602996E-5	2.968812035801498E-5
22-23	0.0	2.1771288262544317E-4	0.0	6.927228083536829E-5	4.9480200596691636E-5
24-25	0.0	2.42452982923789E-4	0.0	1.8802476226742823E-4	5.937624071602996E-5
26-27	0.0	2.572970431027965E-4	0.0	4.1068566495254056E-4	5.937624071602996E-5
28-29	0.0	2.572970431027965E-4	0.0	0.0017763392014212298	5.937624071602996E-5
30-31	9.896040119338327E-6	3.1667328381882647E-4	0.0	0.005042032440802878	6.432426077569912E-5
32-33	9.896040119338327E-6	3.3151734399783394E-4	0.0	0.00941113415349075	6.927228083536829E-5
34-35	9.896040119338327E-6	3.8594556465419477E-4	0.0	0.015813872110702648	6.927228083536829E-5
36-37	9.896040119338327E-6	4.255297251315481E-4	0.0	0.026704464262034475	6.927228083536829E-5
38-39	9.896040119338327E-6	4.3047774519121724E-4	0.0	0.03911904659174441	6.927228083536829E-5
40-41	9.896040119338327E-6	4.7006190566857055E-4	0.0	0.049208059493409825	6.927228083536829E-5
42-43	9.896040119338327E-6	4.997500260265856E-4	0.0	0.05938118873608963	6.927228083536829E-5
44-45	9.896040119338327E-6	5.44282206563608E-4	0.0	0.06773344659681119	6.927228083536829E-5
46-47	9.896040119338327E-6	5.640742868022846E-4	0.0	0.07585314751472827	7.422030089503746E-5
48-49	9.896040119338327E-6	5.690223068619538E-4	0.0	0.0829733483805922	7.916832095470662E-5
50-51	9.896040119338327E-6	6.333465676376529E-4	0.0	0.09002427696562076	7.916832095470662E-5
52-53	9.896040119338327E-6	6.333465676376529E-4	0.0	0.09719395803208138	8.906436107404494E-5
54-55	9.896040119338327E-6	6.382945876973221E-4	0.0	0.10338887914678718	9.896040119338327E-5
56-57	9.896040119338327E-6	6.67982708055337E-4	0.0	0.10887623339296026	1.0390842125305243E-4
58-59	9.896040119338327E-6	6.927228083536829E-4	0.0	0.11433389951877536	1.088564413127216E-4
60-61	9.896040119338327E-6	7.125148885923596E-4	0.0	0.1198311498050678	1.088564413127216E-4
62-63	9.896040119338327E-6	7.323069688310362E-4	0.0	0.12480390996503532	1.088564413127216E-4
64-65	9.896040119338327E-6	7.619950891890512E-4	0.0	0.1301972518300747	1.088564413127216E-4
66-67	9.896040119338327E-6	7.669431092487204E-4	0.0	0.13643175710525784	1.1380446137239077E-4
68-69	9.896040119338327E-6	8.015792496664045E-4	0.0	0.14262173019990398	1.1875248143205993E-4
70-71	9.896040119338327E-6	8.114752897857428E-4	0.0	0.14784189136285492	1.1875248143205993E-4
72-73	9.896040119338327E-6	8.114752897857428E-4	0.0	0.1524682901186456	1.2864852155139824E-4
74-75	9.896040119338327E-6	8.16423309845412E-4	0.0	0.15586757989963831	1.2864852155139824E-4
76-77	9.896040119338327E-6	8.510594502630962E-4	0.0	0.15902441669770725	1.2864852155139824E-4
78-79	9.896040119338327E-6	8.708515305017728E-4	0.0	0.16120649354402133	1.2864852155139824E-4
80-81	9.896040119338327E-6	8.708515305017728E-4	0.0	0.16203776091404576	1.2864852155139824E-4
82-83	9.896040119338327E-6	8.708515305017728E-4	0.0	0.1627057436221011	1.2864852155139824E-4
84-85	9.896040119338327E-6	8.807475706211111E-4	0.0	0.16299272878556192	1.2864852155139824E-4
86-87	9.896040119338327E-6	9.005396508597877E-4	0.0	0.16309663720681497	1.2864852155139824E-4
88	9.896040119338327E-6	9.302277712178028E-4	0.0	0.16309663720681497	1.2864852155139824E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20715	0.0	51.911606	1
GTATCAA	39135	0.0	37.837425	1
TCAACGC	48860	0.0	29.95173	4
ATCAACG	48595	0.0	29.94594	3
TATCAAC	49470	0.0	29.616158	2
CAACGCA	49805	0.0	29.34656	5
AACGCAG	50960	0.0	28.757992	6
GTGGTAT	8615	0.0	27.73841	1
TGGTATC	8810	0.0	25.674696	2
ACGCAGA	58110	0.0	25.219536	7
CGCAGAG	58835	0.0	24.901766	8
GCAGAGT	65330	0.0	22.356722	9
GTACATG	43900	0.0	20.74133	1
GAGTACT	40735	0.0	20.399893	12-13
TACATGG	44250	0.0	20.214148	2
CTTATAC	6755	0.0	19.578909	1
CAGAGTA	64720	0.0	19.448982	10-11
ACATGGG	45095	0.0	19.38768	3
AGAGTAC	61835	0.0	18.757732	10-11
GTACTTT	44850	0.0	18.423403	14-15
>>END_MODULE