FastQCFastQC Report
Fri 27 May 2016
SRR2139049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9012550
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127300.048.365991
GTATCAA239150.037.685161
TCAACGC298200.029.5231674
ATCAACG298800.029.44953
CAACGCA303300.029.0270545
AACGCAG309150.028.5739886
TATCAAC312200.028.5135442
CGCAGAG356600.024.6765488
ACGCAGA358000.024.6156527
GCAGAGT411950.021.319969
TACATGG271950.020.2027052
GTACATG278350.020.1656251
AGAGTAC372400.019.83052310-11
GAGTACT246250.019.618912-13
ACATGGG273450.019.5315063
AGTACTT266850.018.39895612-13
CAGAGTA402500.018.32115410-11
CATGGGG207400.017.4341854
GTACTTT283800.016.93425214-15
TGGTATC65350.016.905492
Produced by FastQC (version 0.11.5)