##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139049_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9012550
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66626371004877	34.0	31.0	34.0	31.0	34.0
2	32.81317107810775	34.0	31.0	34.0	31.0	34.0
3	32.85999822469778	34.0	31.0	34.0	31.0	34.0
4	36.32473883640035	37.0	37.0	37.0	35.0	37.0
5	36.26211349728989	37.0	37.0	37.0	35.0	37.0
6	36.29735724073653	37.0	37.0	37.0	35.0	37.0
7	36.25086207566116	37.0	37.0	37.0	35.0	37.0
8	36.2337182040599	37.0	37.0	37.0	35.0	37.0
9	37.91279848655486	39.0	38.0	39.0	35.0	39.0
10-11	37.88494122085314	39.0	38.0	39.0	35.0	39.0
12-13	37.85769133042258	39.0	38.0	39.0	35.0	39.0
14-15	39.268557455991925	41.0	39.0	41.0	36.0	41.0
16-17	39.25451220797666	41.0	39.0	41.0	36.0	41.0
18-19	39.28800999717061	41.0	39.0	41.0	36.0	41.0
20-21	39.20755265901771	41.0	39.0	41.0	36.0	41.0
22-23	39.20083243326201	40.0	39.0	41.0	36.0	41.0
24-25	39.10595897379402	40.0	39.0	41.0	35.5	41.0
26-27	38.963943616312335	40.0	39.0	41.0	35.0	41.0
28-29	38.83489230837996	40.0	38.0	41.0	35.0	41.0
30-31	38.71406906747734	40.0	38.0	41.0	35.0	41.0
32-33	38.62152903785948	40.0	38.0	41.0	35.0	41.0
34-35	38.48205006778153	40.0	38.0	41.0	34.5	41.0
36-37	38.621876079337326	40.0	38.0	41.0	35.0	41.0
38-39	38.682645247823054	40.0	38.0	41.0	35.0	41.0
40-41	38.66502859617144	40.0	38.0	41.0	35.0	41.0
42-43	38.631424929355504	40.0	38.0	41.0	35.0	41.0
44-45	38.55745198662116	40.0	38.0	41.0	34.0	41.0
46-47	38.495983793363365	40.0	38.0	41.0	34.0	41.0
48-49	38.40744439652974	40.0	38.0	41.0	34.0	41.0
50-51	38.31002923978874	40.0	38.0	41.0	34.0	41.0
52-53	38.19810527374401	40.0	37.5	41.0	34.0	41.0
54-55	38.058209251417125	40.0	37.0	41.0	33.0	41.0
56-57	37.90499045103733	40.0	37.0	41.0	33.0	41.0
58-59	37.713996299779005	39.5	36.0	41.0	33.0	41.0
60-61	37.48279990317546	39.0	36.0	41.0	33.0	41.0
62-63	37.283512523433444	39.0	35.5	40.0	32.5	41.0
64-65	37.04799374783981	38.5	35.0	40.0	32.0	41.0
66-67	36.77219836706376	38.0	35.0	40.0	32.0	41.0
68-69	36.41792202811419	37.0	35.0	40.0	31.0	41.0
70-71	36.1446147876043	37.0	35.0	39.5	31.0	41.0
72-73	35.820830520181374	36.0	34.5	39.0	31.0	40.5
74-75	35.48959318879406	36.0	34.0	39.0	31.0	40.0
76-77	34.46334088963167	35.0	33.5	37.0	29.5	39.0
78-79	34.75849831963792	35.0	34.0	37.0	30.5	39.0
80-81	34.59582482430251	35.0	34.0	37.0	30.5	39.0
82-83	34.32096000160706	35.0	34.0	36.0	30.0	38.5
84-85	34.05655903619602	35.0	34.0	36.0	30.0	37.0
86-87	33.831718747352625	35.0	34.0	35.5	30.0	37.0
88-89	33.647047756754134	35.0	33.0	35.0	30.0	36.5
90-91	33.455738587222044	35.0	33.0	35.0	30.0	36.0
92-93	33.31665536118367	35.0	33.0	35.0	29.0	36.0
94-95	33.228613429201616	34.0	33.0	35.0	29.0	35.5
96-97	33.21030084235789	34.0	33.0	35.0	29.0	35.0
98-99	33.21814146562223	34.0	33.0	35.0	30.0	35.0
100-101	32.214441554837364	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	35.0
21	251.0
22	1059.0
23	3582.0
24	9372.0
25	19374.0
26	34663.0
27	54275.0
28	77540.0
29	104589.0
30	134858.0
31	169733.0
32	215186.0
33	280968.0
34	397360.0
35	578400.0
36	1006896.0
37	2060995.0
38	2646288.0
39	1170334.0
40	46787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.877791399525265	21.618134891693792	15.125765196949276	24.37830851183167
2	13.552371102904623	22.22045664370428	41.502678698245866	22.72449355514523
3	16.362574006532444	27.76237887860582	31.84262299156432	24.032424123297417
4	11.618281892819187	19.323196796998317	39.89865904706617	29.159862263116327
5	12.473230381327191	38.43065929516722	36.167592542354	12.92851778115159
6	28.277094526753753	39.308635104230774	19.420698850014574	12.993571519000897
7	24.95977553076272	33.5158788040986	24.251385484838973	17.272960180299712
8	23.97363676207067	36.73812627946585	21.816100881548508	17.472136076914968
9	25.488546526787648	16.641094917642622	21.743812794381167	36.12654576118857
10-11	23.438522192761866	27.113966753686665	29.775379451268062	19.67213160228341
12-13	24.59039894369518	24.928005947262427	29.58532823673655	20.89626687230584
14-15	21.77551622181581	26.110284325259066	27.463058853683687	24.65114059924144
16-17	21.065737673440736	29.166853675855386	28.025414312963008	21.74199433774087
18-19	21.08615763574127	28.324602914824325	29.888949298478234	20.700290150956167
20-21	22.07217705973977	27.41191466579378	29.685734773786127	20.830173500680328
22-23	21.723596999626135	27.40984990251659	29.515139752112823	21.35141334574445
24-25	21.55371001620736	27.59777059695285	29.623463278242806	21.225056108596984
26-27	21.537456090460896	27.774246023138765	29.45315666119467	21.235141225205666
28-29	21.45584162918371	27.806295353636457	29.490032997635385	21.247830019544452
30-31	21.60910611471282	27.72605080436837	29.48491421169913	21.179928869219676
32-33	21.525070133848327	27.830685367832686	29.393068840575857	21.251175657743126
34-35	21.659357604719457	27.770121768856164	29.373396195714278	21.197124430710105
36-37	21.555582712511807	27.82015238203266	29.31091174004221	21.31335316541332
38-39	21.457320992648405	27.848628728059943	29.35516946622702	21.338880813064627
40-41	21.63186722957023	27.78965698919581	29.182666538941387	21.395809242292575
42-43	21.616165296102345	27.870019613201823	29.0112652365479	21.50254985414793
44-45	21.69558702484807	28.02221938162392	28.7401380716369	21.542055521891115
46-47	21.797397157374046	27.900956776745566	28.385558975244003	21.916087090636385
48-49	21.7654675128235	28.098229965282258	28.30133200470626	21.834970517187987
50-51	21.716583931160777	28.173998693432907	28.193806934127114	21.915610441279203
52-53	21.840956053316614	28.11899516776457	28.08741511845319	21.95263366046562
54-55	21.8344842555947	28.071983962719855	28.067492203459217	22.026039578226232
56-57	21.94374527475109	28.17314370982027	27.973614326400714	21.90949668902792
58-59	21.81308193271758	28.36142222867802	28.06602008230648	21.75947575629792
60-61	22.014985710017257	28.318218738773883	27.88880576995636	21.7779897812525
62-63	21.927276206723644	28.38752837227034	27.81882560804548	21.86636981296054
64-65	21.83827014552511	28.315853964685477	27.906624269292728	21.939251620496684
66-67	21.953668708353828	28.351764059363273	27.72670978615957	21.967857446123332
68-69	21.93919207571129	28.17821491390564	27.864531771239154	22.018061239143915
70-71	21.953888897065195	27.975804671287918	27.902172117810775	22.16813431383611
72-73	22.027455092136538	27.995465661249696	27.84954446200047	22.127534784613296
74-75	21.894535964085883	28.12124123964925	27.813835933892385	22.17038686237248
76-77	21.99634976196502	28.030407779268273	27.762326999120525	22.210915459646177
78-79	22.003806263995017	28.06351689424545	27.68456540341333	22.248111438346204
80-81	21.99505297205485	28.067061976364442	27.764517944972454	22.173367106608254
82-83	22.021277017956116	27.957838108144443	27.883120461265015	22.137764412634418
84-85	22.17548906744847	28.01097842641194	27.761662694481515	22.05186981165808
86-87	21.94725284901934	28.005178901851973	27.821082119787928	22.22648612934076
88-89	22.052710279268304	27.895569628242136	27.8501355772566	22.20158451523296
90-91	22.09053105334355	27.954820093954563	27.90025248213903	22.054396370562852
92-93	22.19106402078988	27.896599498539732	27.865681261024733	22.046655219645654
94-95	21.98282620219488	27.924318304934197	27.8892047881986	22.203650704672324
96-97	21.8813772530912	27.94592980657407	28.026306245835574	22.14638669449915
98-99	22.13857708824016	28.541809357016323	28.061707065501796	21.257906489241712
100-101	22.70283185177588	31.24023495918813	25.20173446528597	20.855198723750018
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2406.0
1	2189.0
2	3183.0
3	4641.5
4	6415.5
5	9918.0
6	12536.0
7	14511.0
8	15757.5
9	15470.5
10	13995.5
11	12548.0
12	13793.5
13	18761.0
14	30625.5
15	46747.0
16	59126.5
17	64936.5
18	65133.5
19	60998.0
20	54685.0
21	48797.0
22	46346.0
23	50031.0
24	59414.5
25	74670.0
26	94164.5
27	115016.5
28	135581.5
29	157691.5
30	178268.0
31	197169.5
32	218937.5
33	240694.0
34	261571.0
35	280052.5
36	299489.0
37	316139.5
38	325065.5
39	329487.5
40	335223.5
41	341908.0
42	344504.0
43	348497.0
44	354431.0
45	360633.0
46	365714.0
47	364942.0
48	358344.5
49	350799.0
50	341393.5
51	329485.5
52	315287.0
53	298962.5
54	282509.5
55	262039.0
56	239109.0
57	214192.5
58	188439.0
59	164071.0
60	137851.5
61	111027.0
62	85809.5
63	63963.5
64	46477.0
65	34135.5
66	25895.5
67	19497.0
68	14312.5
69	9827.5
70	6666.0
71	4785.5
72	3290.0
73	2195.0
74	1437.0
75	921.0
76	600.0
77	392.0
78	250.5
79	152.5
80	94.5
81	61.0
82	45.5
83	35.5
84	26.5
85	16.5
86	10.0
87	8.0
88	5.0
89	3.5
90	3.5
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1095638859146413E-5
2	0.007877903589993953
3	1.1095638859146413E-5
4	4.438255543658565E-5
5	1.1095638859146413E-5
6	1.1095638859146413E-5
7	1.1095638859146413E-5
8	0.0
9	0.0
10-11	6.879296092670775E-4
12-13	0.0
14-15	8.044338172881148E-4
16-17	7.711469007106756E-4
18-19	0.0
20-21	4.1055232658616475E-4
22-23	0.0013039047397159229
24-25	0.004345528692393788
26-27	0.017072785197545452
28-29	0.32050276075672984
30-31	0.30780638168692204
32-33	0.3176677617163306
34-35	0.18278339275281394
36-37	0.04377704571079435
38-39	0.03414952374348517
40-41	0.030314157965834497
42-43	0.026741778798784574
44-45	0.01460748572464756
46-47	0.007655316165729975
48-49	0.00577381702483677
50-51	0.002288501528635368
52-53	0.0017828586572544577
54-55	0.0014264167155644339
56-57	5.509125529771284E-4
58-59	0.0027296409237648343
60-61	0.002336691730541433
62-63	3.8977928308022195E-4
64-65	2.0301568378632373E-4
66-67	0.00823442939889947
68-69	0.007684960390082633
70-71	0.009725589261484403
72-73	0.010113308691473933
74-75	0.008024715858019229
76-77	0.0053633494036171075
78-79	4.017041242145408E-4
80-81	6.677871722352545E-4
82-83	7.241244065226063E-4
84-85	0.0
86-87	0.0018449942669830656
88-89	0.001435346304343906
90-91	0.0011552436105340376
92-93	0.011939871430811414
94-95	1.2066486036398404E-4
96-97	3.0733740391480234E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	171.0
20-21	688.0
22-23	1383.0
24-25	3725.0
26-27	4646.0
28-29	8123.0
30-31	21890.0
32-33	28807.0
34-35	40014.0
36-37	54058.0
38-39	61784.0
40-41	65499.0
42-43	77298.0
44-45	91034.0
46-47	89932.0
48-49	77895.0
50-51	68849.0
52-53	62780.0
54-55	67498.0
56-57	72300.0
58-59	71769.0
60-61	71916.0
62-63	70643.0
64-65	76560.0
66-67	80710.0
68-69	78457.0
70-71	76592.0
72-73	73371.0
74-75	74848.0
76-77	75691.0
78-79	80204.0
80-81	82344.0
82-83	80165.0
84-85	81113.0
86-87	85234.0
88-89	91919.0
90-91	99478.0
92-93	104171.0
94-95	116888.0
96-97	172395.0
98-99	750157.0
100-101	5619551.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.03836224859359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.19340872276172	72.352428127362
2	7.73388026470307	12.689497414859188
3	2.141234608383836	5.269901413854557
4	0.8720820419922414	2.861767298858108
5	0.38692160786595964	1.5871207513957937
6	0.21656762679084174	1.0660112050791177
7	0.1362313498600787	0.782333778060522
8	0.08528347720433292	0.5597213437366991
9	0.05942264164225157	0.4387442580733857
>10	0.1719565474792361	2.135412300697376
>50	0.002476054098532934	0.13408666284983514
>100	4.61549562005006E-4	0.07254080834821754
>500	4.9005121873693574E-5	0.026875705100749027
>1k	2.4502533972708037E-5	0.023558931724480696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.328691657743924E-5	0.0	0.0	0.0
3	0.0	3.328691657743924E-5	0.0	0.0	0.0
4	0.0	1.775302217463426E-4	0.0	0.0	0.0
5	0.0	1.775302217463426E-4	0.0	0.0	0.0
6	0.0	1.775302217463426E-4	0.0	0.0	1.1095638859146413E-5
7	0.0	1.775302217463426E-4	0.0	0.0	1.1095638859146413E-5
8	0.0	1.9972149946463543E-4	0.0	0.0	1.1095638859146413E-5
9	0.0	3.661560823518316E-4	0.0	0.0	1.1095638859146413E-5
10-11	0.0	3.661560823518316E-4	0.0	1.1095638859146413E-5	1.1095638859146413E-5
12-13	0.0	3.661560823518316E-4	0.0	1.664345828871962E-5	1.1095638859146413E-5
14-15	0.0	4.993037486615885E-4	0.0	3.328691657743924E-5	2.7739097147866032E-5
16-17	0.0	4.993037486615885E-4	0.0	7.212165258445168E-5	3.328691657743924E-5
18-19	0.0	5.38138484668601E-4	0.0	1.4424330516890337E-4	3.328691657743924E-5
20-21	0.0	5.769732206756135E-4	0.0	2.718431520490871E-4	3.328691657743924E-5
22-23	0.0	6.324514149713455E-4	0.0	9.209380253091523E-4	3.8834736007012445E-5
24-25	0.0	6.879296092670776E-4	0.0	0.0023189885215616005	4.993037486615886E-5
26-27	0.0	6.99025248126224E-4	0.0	0.006041575358805222	5.5478194295732064E-5
28-29	0.0	7.156687064149436E-4	0.0	0.025769621250367546	5.5478194295732064E-5
30-31	0.0	8.931989281612862E-4	0.0	0.03624945215283133	5.5478194295732064E-5
32-33	0.0	9.264858447387255E-4	0.0	0.040027517184370684	6.657383315487848E-5
34-35	0.0	0.0010929204276259217	0.0	0.04533678037847224	6.657383315487848E-5
36-37	0.0	0.0012538071910835446	0.0	0.05253784999805826	6.657383315487848E-5
38-39	0.0	0.0012981897465201302	0.0	0.06533112160265409	6.657383315487848E-5
40-41	0.0	0.0014202417739707408	0.0	0.09086218661754997	6.657383315487848E-5
42-43	0.0	0.0014646243294073264	0.0	0.10267349418311134	6.657383315487848E-5
44-45	0.0	0.0016310589122945228	0.0	0.11792999761443765	7.212165258445168E-5
46-47	0.0	0.0017531109397451333	0.0	0.13627108864860665	8.32172914435981E-5
48-49	0.0	0.0017531109397451333	0.0	0.15252619957725616	9.986074973231771E-5
50-51	0.0	0.0019528324392097686	0.0	0.16724456452391387	9.986074973231771E-5
52-53	0.0	0.0019750237169280614	0.0	0.18783252242706006	1.0540856916189092E-4
54-55	0.0	0.001991667175216781	0.0	0.22810414366633194	1.1650420802103733E-4
56-57	0.0	0.002063788827801233	0.0	0.2701344236647786	1.2759984688018376E-4
58-59	0.0	0.002063788827801233	0.0	0.3042868000732312	1.3869548573933015E-4
60-61	0.0	0.002080432286089952	0.0	0.32549611375248955	1.4424330516890337E-4
62-63	0.0	0.0021303626609561113	0.0	0.3440480219249824	1.664345828871962E-4
64-65	0.0	0.0025686403958923945	0.0	0.35329068909465133	1.664345828871962E-4
66-67	0.0	0.002585283854181114	0.0	0.35892172581566817	1.719824023167694E-4
68-69	0.0	0.002696240242772578	0.0	0.3645194756201075	1.775302217463426E-4
70-71	0.0	0.002735074978779591	0.0	0.3698675735502161	1.8862586060548902E-4
72-73	0.0	0.0027517184370683106	0.0	0.37420042052471275	1.8862586060548902E-4
74-75	0.0	0.0028349357285119086	0.0	0.3774015123355765	1.8862586060548902E-4
76-77	0.0	0.002951439936532946	0.0	0.38045281302184175	1.8862586060548902E-4
78-79	0.0	0.0030346572279765435	0.0	0.38337651386122684	1.8862586060548902E-4
80-81	0.0	0.003051300686265263	0.0	0.38460812977459213	1.8862586060548902E-4
82-83	0.0	0.0030790397834131295	0.0	0.3850186684123805	1.9972149946463543E-4
84-85	0.0	0.003095683241701849	0.0	0.38526277246728174	1.9972149946463543E-4
86-87	5.547819429573206E-6	0.003128970158279288	0.0	0.3853293463004366	1.9972149946463543E-4
88-89	1.1095638859146413E-5	0.0033065003800256307	0.0	0.38536263321701403	1.9972149946463543E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12730	0.0	48.36599	1
GTATCAA	23915	0.0	37.68516	1
TCAACGC	29820	0.0	29.523167	4
ATCAACG	29880	0.0	29.4495	3
CAACGCA	30330	0.0	29.027054	5
AACGCAG	30915	0.0	28.573988	6
TATCAAC	31220	0.0	28.513544	2
CGCAGAG	35660	0.0	24.676548	8
ACGCAGA	35800	0.0	24.615652	7
GCAGAGT	41195	0.0	21.31996	9
TACATGG	27195	0.0	20.202705	2
GTACATG	27835	0.0	20.165625	1
AGAGTAC	37240	0.0	19.830523	10-11
GAGTACT	24625	0.0	19.6189	12-13
ACATGGG	27345	0.0	19.531506	3
AGTACTT	26685	0.0	18.398956	12-13
CAGAGTA	40250	0.0	18.321154	10-11
CATGGGG	20740	0.0	17.434185	4
GTACTTT	28380	0.0	16.934252	14-15
TGGTATC	6535	0.0	16.90549	2
>>END_MODULE