FastQCFastQC Report
Fri 27 May 2016
SRR2139048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9207076
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137000.050.8606381
GTATCAA247300.036.8692321
CTTATAC109600.029.2681241
TCAACGC312250.028.7363174
ATCAACG313700.028.5627043
CAACGCA315700.028.3553035
TATCAAC323700.027.8916842
AACGCAG326300.027.4602096
TATACAC145550.024.3147643
ACGCAGA372750.024.0268757
CGCAGAG375600.023.8898098
TTATACA150250.022.0000482
GCAGAGT433250.020.6524319
GTACATG287750.020.051321
TACATGG282450.019.810832
GAGTACT265050.019.41178112-13
ACATCTC166750.019.3129088
AGAGTAC399150.019.3034110-11
TACACAT183650.019.1319355
ACACATC168550.018.955426
Produced by FastQC (version 0.11.5)