##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9207076
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68213013556095	34.0	31.0	34.0	31.0	34.0
2	32.82419945268183	34.0	31.0	34.0	31.0	34.0
3	32.86948494831584	34.0	31.0	34.0	31.0	34.0
4	36.332538582281714	37.0	37.0	37.0	35.0	37.0
5	36.27061381919732	37.0	37.0	37.0	35.0	37.0
6	36.30463786765744	37.0	37.0	37.0	35.0	37.0
7	36.25660111853101	37.0	37.0	37.0	35.0	37.0
8	36.23774670698928	37.0	37.0	37.0	35.0	37.0
9	37.91839341827959	39.0	38.0	39.0	35.0	39.0
10-11	37.89032614697652	39.0	38.0	39.0	35.0	39.0
12-13	37.86131867489744	39.0	38.0	39.0	35.0	39.0
14-15	39.27616482149165	41.0	39.0	41.0	36.0	41.0
16-17	39.26159195384072	41.0	39.0	41.0	36.0	41.0
18-19	39.29607722364842	41.0	39.0	41.0	36.0	41.0
20-21	39.21700085411341	41.0	39.0	41.0	36.0	41.0
22-23	39.210169347240324	40.5	39.0	41.0	36.0	41.0
24-25	39.11102505920627	40.0	39.0	41.0	35.5	41.0
26-27	38.96953096658232	40.0	39.0	41.0	35.0	41.0
28-29	38.835613104184915	40.0	38.0	41.0	35.0	41.0
30-31	38.71136217541869	40.0	38.0	41.0	35.0	41.0
32-33	38.618758790443195	40.0	38.0	41.0	35.0	41.0
34-35	38.4747756616209	40.0	38.0	41.0	34.5	41.0
36-37	38.61092249960197	40.0	38.0	41.0	35.0	41.0
38-39	38.66607369371495	40.0	38.0	41.0	35.0	41.0
40-41	38.65794283134704	40.0	38.0	41.0	35.0	41.0
42-43	38.628656260340996	40.0	38.0	41.0	35.0	41.0
44-45	38.55513565736658	40.0	38.0	41.0	34.0	41.0
46-47	38.48576511576995	40.0	38.0	41.0	34.0	41.0
48-49	38.39981286925041	40.0	38.0	41.0	34.0	41.0
50-51	38.2905084360236	40.0	38.0	41.0	34.0	41.0
52-53	38.18247728950841	40.0	37.5	41.0	34.0	41.0
54-55	38.044392555683295	40.0	37.0	41.0	33.0	41.0
56-57	37.88036887378766	40.0	37.0	41.0	33.0	41.0
58-59	37.68928376839682	39.5	36.0	41.0	33.0	41.0
60-61	37.45839487506132	39.0	36.0	41.0	33.0	41.0
62-63	37.26028651978434	39.0	35.0	40.0	32.5	41.0
64-65	37.02988846937279	38.5	35.0	40.0	32.0	41.0
66-67	36.7548999658248	38.0	35.0	40.0	32.0	41.0
68-69	36.408233241474726	37.0	35.0	40.0	31.0	41.0
70-71	36.1389665820526	37.0	35.0	39.5	31.0	41.0
72-73	35.81861508310321	36.0	34.5	39.0	31.0	40.5
74-75	35.49215858678562	36.0	34.0	39.0	31.0	40.0
76-77	34.47288418322928	35.0	33.5	37.0	29.5	39.0
78-79	34.767082601292046	35.0	34.0	37.0	30.5	39.0
80-81	34.60378340866278	35.0	34.0	37.0	30.5	39.0
82-83	34.33033340324303	35.0	34.0	36.0	30.0	38.5
84-85	34.06837333237132	35.0	34.0	36.0	30.0	37.0
86-87	33.84532394410603	35.0	34.0	35.5	30.0	37.0
88-89	33.66236656518292	35.0	33.0	35.0	30.0	36.5
90-91	33.4735983495989	35.0	33.0	35.0	30.0	36.0
92-93	33.333999909943955	35.0	33.0	35.0	29.5	36.0
94-95	33.24457776222555	34.0	33.0	35.0	29.0	35.5
96-97	33.224335333374256	34.0	33.0	35.0	29.5	35.0
98-99	33.231529510722496	34.0	33.0	35.0	30.0	35.0
100-101	32.23841867364416	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	1.0
17	1.0
18	1.0
19	8.0
20	34.0
21	243.0
22	1130.0
23	3710.0
24	9664.0
25	19741.0
26	34821.0
27	55225.0
28	79637.0
29	106988.0
30	138178.0
31	173374.0
32	220487.0
33	287249.0
34	407456.0
35	592422.0
36	1030763.0
37	2094765.0
38	2707170.0
39	1197233.0
40	46772.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.522933561737425	21.555360584698242	15.35683323496694	24.5648726185974
2	13.470368265948235	21.947841943664397	41.72644506848144	22.855344721905926
3	16.242217668718638	27.60716379601163	32.02777559950099	24.122842935768738
4	11.663113237979076	19.433793812798207	39.31212644196253	29.59096650726018
5	12.51552882055689	38.30728415998394	36.12779695264749	13.049390066811672
6	28.43816613182429	39.044206661095586	19.21760376858055	13.300023438499572
7	24.99888672557644	33.4515721281267	23.965203277393012	17.584337868903845
8	24.05544387816501	36.76984962435414	21.738454206308276	17.436252291172572
9	25.434187792085133	16.646196903338257	21.49693344553689	36.42268185903973
10-11	23.48520085984535	27.0649536448038	29.535990777456444	19.913854717894402
12-13	24.612936247396703	24.80487559838494	29.402578994957686	21.179609159260675
14-15	21.8167584944177	25.998048767772136	27.164400140002133	25.02079259780803
16-17	21.179576662621017	29.219605742648273	27.713834024120654	21.88698357061006
18-19	21.308470860263935	28.135475808795096	29.68423578149218	20.87181754944879
20-21	22.13705170299342	27.215831325659767	29.534301918840278	21.112815052506534
22-23	21.85071739691038	27.422451548036143	29.366059151924162	21.36077190312931
24-25	21.82430137422715	27.536682022782742	29.450944782524317	21.188071820465787
26-27	21.749368324519345	27.771206252161733	29.32905553922715	21.15036988409177
28-29	21.590147283521937	27.68201614883378	29.419734443751818	21.308102123892464
30-31	21.6328064139814	27.676267688787128	29.521740714344276	21.1691851828872
32-33	21.663251758345083	27.745961708666673	29.36527613001811	21.225510402970134
34-35	21.845244949211935	27.567182756130464	29.421748116625995	21.1658241780316
36-37	21.555146899070323	27.828056379468148	29.23400186687637	21.382794854585157
38-39	21.51664802254852	27.56167633899574	29.38118487028704	21.540490768168706
40-41	21.572542286668263	27.507563304637973	29.327946797822918	21.591947610870843
42-43	21.68762201017428	27.587701104485923	29.16960983288965	21.55506705245015
44-45	21.674844302535295	27.824275500511277	28.85832529645633	21.642554900497103
46-47	21.930851645050335	27.60891742067854	28.387128229292646	22.073102704978474
48-49	21.859249910958628	27.776133852866398	28.381041566488484	21.983574669686494
50-51	21.723300970873787	27.970498035197622	28.339737315532343	21.96646367839625
52-53	21.8288065831013	27.97168755486551	28.319238603543223	21.880267258489965
54-55	21.805607952957878	27.966384676612734	28.155050949274685	22.072956421154704
56-57	22.04189834103613	28.105720234007258	27.878927553972037	21.973453870984567
58-59	21.71932660556371	28.383079615675438	28.22134930753903	21.676244471221818
60-61	22.062723130183482	28.45043776256736	27.78453010448891	21.702309002760252
62-63	21.82560586117927	28.599337892595205	27.763117202696957	21.811939043528568
64-65	21.82640527649711	28.456779439550488	27.852962397234567	21.863852886717837
66-67	21.942205728306856	28.352513204665442	27.715290052096282	21.989991014931423
68-69	21.942648220408163	28.14975447103023	27.86725169704034	22.040345611521268
70-71	21.9302745993437	27.989933318523086	27.935195416496406	22.144596665636808
72-73	22.02226803202652	27.955620285765654	27.884522673028698	22.137589009179123
74-75	21.949329914482178	28.048896808869035	27.819347621682017	22.182425654966778
76-77	22.073316872458495	28.025288172333152	27.72729505476006	22.174099900448294
78-79	22.02325522452768	27.989333837653728	27.72352239351743	22.26388854430116
80-81	22.046604749006605	27.962058459452365	27.820188703135607	22.171148088405424
82-83	22.0532923417482	27.898270763200316	27.941266539360893	22.107170355690588
84-85	22.172016627628494	27.934883069665844	27.810300949515355	22.08279935319031
86-87	22.057511083781208	27.972570918512655	27.80301273035662	22.166905267349513
88-89	22.09159986371646	27.850244660659225	27.848277127225668	22.209878348398647
90-91	22.12092173537471	27.913544114243443	27.86249262790861	22.103041522473234
92-93	22.213298251356967	27.835465394986265	27.848041110362892	22.10319524329388
94-95	22.009774532249967	27.875846733086963	27.94845712165086	22.16592161301221
96-97	21.9480011390525	27.910370241756517	28.004503202794563	22.137125416396422
98-99	22.217474131510066	28.48307653750139	28.017083114115472	21.282366216873072
100-101	22.76705798932944	31.12388904561336	25.232118382455145	20.876934582602054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2561.0
1	2364.0
2	3395.5
3	4866.0
4	6443.5
5	9679.0
6	12332.5
7	14950.0
8	17130.0
9	17259.5
10	15676.5
11	13898.0
12	14915.5
13	19749.0
14	31520.0
15	47569.5
16	59971.0
17	66000.5
18	66157.5
19	62293.0
20	56569.5
21	51249.5
22	49123.5
23	52842.5
24	62343.0
25	77246.5
26	95919.0
27	115346.0
28	136053.0
29	159022.5
30	179085.0
31	197588.5
32	218716.5
33	238872.5
34	257515.0
35	274479.5
36	293388.0
37	309620.5
38	318046.0
39	322810.0
40	329128.5
41	337695.5
42	343935.0
43	350754.0
44	358967.0
45	368615.0
46	379974.5
47	386502.5
48	380958.5
49	372392.5
50	364788.5
51	349978.5
52	330142.5
53	311004.0
54	292285.0
55	270016.5
56	245940.0
57	220632.5
58	194568.5
59	167643.5
60	140437.5
61	115531.5
62	91567.5
63	69612.5
64	51877.0
65	39570.0
66	30720.5
67	23440.0
68	17809.0
69	12547.5
70	8470.0
71	6020.5
72	4198.5
73	2884.0
74	1951.0
75	1257.0
76	835.0
77	534.5
78	332.5
79	217.5
80	147.5
81	103.0
82	78.5
83	59.0
84	42.5
85	31.5
86	26.0
87	22.5
88	17.0
89	11.5
90	6.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.1722423058091407E-5
2	0.007744043820209587
3	2.1722423058091407E-5
4	6.516726917427421E-5
5	2.1722423058091407E-5
6	2.1722423058091407E-5
7	2.1722423058091407E-5
8	0.0
9	0.0
10-11	7.765766243267678E-4
12-13	1.0861211529045703E-5
14-15	7.982990473848592E-4
16-17	7.548542012686764E-4
18-19	5.430605764522852E-6
20-21	4.23600039883573E-4
22-23	0.0011405571589133945
24-25	0.004525354671276988
26-27	0.016712152707879614
28-29	0.32161602126952793
30-31	0.30954518185745405
32-33	0.3186263658486428
34-35	0.18468890467969679
36-37	0.04626773256953316
38-39	0.03555749194360752
40-41	0.03203614471611439
42-43	0.027926033512027337
44-45	0.014805866936853403
46-47	0.007616734631302153
48-49	0.005596821329938006
50-51	0.0022154099826607633
52-53	0.0017852423033372954
54-55	0.0013062071319146894
56-57	4.312198628972381E-4
58-59	0.00266611208311015
60-61	0.00243529500516722
62-63	3.2041128979038385E-4
64-65	8.090718613893108E-5
66-67	0.007619643503873246
68-69	0.006687929318450367
70-71	0.008981790304192728
72-73	0.00956317519230552
74-75	0.007453106391815506
76-77	0.00478603795559788
78-79	3.744939399858749E-4
80-81	5.137732814503442E-4
82-83	6.220335918646208E-4
84-85	0.0
86-87	0.0019853867148826687
88-89	0.00159240278080236
90-91	0.0011116735621506566
92-93	0.01156279002482689
94-95	1.4045972764563106E-4
96-97	4.517657263414054E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	175.0
20-21	575.0
22-23	1410.0
24-25	4021.0
26-27	4531.0
28-29	8168.0
30-31	22695.0
32-33	29194.0
34-35	40449.0
36-37	54811.0
38-39	62990.0
40-41	66695.0
42-43	78331.0
44-45	91839.0
46-47	90934.0
48-49	78853.0
50-51	69460.0
52-53	63733.0
54-55	69978.0
56-57	78153.0
58-59	78659.0
60-61	77922.0
62-63	79394.0
64-65	83250.0
66-67	92008.0
68-69	82666.0
70-71	76390.0
72-73	73585.0
74-75	74616.0
76-77	75109.0
78-79	79746.0
80-81	81734.0
82-83	80393.0
84-85	81396.0
86-87	86104.0
88-89	91330.0
90-91	99465.0
92-93	103929.0
94-95	116955.0
96-97	173105.0
98-99	765477.0
100-101	5736848.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.71236367156732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.25301732648512	72.11362646894884
2	7.628859859242711	12.467443424357244
3	2.126417397508945	5.212637751083959
4	0.8512630416175613	2.7823486094727485
5	0.4095040279797608	1.6730771029626945
6	0.2385576632291748	1.1695866320652968
7	0.1320780508076898	0.7554686804440787
8	0.0835797255833724	0.5463597545950659
9	0.06293182384090475	0.4628077268582717
>10	0.2102355439793092	2.5788934790573883
>50	0.0031559140787238054	0.16575934893045693
>100	3.4951277358890524E-4	0.04545645682943978
>500	5.011287314701529E-5	0.02653456439452154
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0861211529045703E-5	0.0
3	0.0	0.0	0.0	2.1722423058091407E-5	0.0
4	0.0	1.0861211529045703E-5	0.0	3.2583634587137114E-5	0.0
5	0.0	1.0861211529045703E-5	0.0	3.2583634587137114E-5	0.0
6	0.0	1.0861211529045703E-5	0.0	4.3444846116182814E-5	0.0
7	0.0	1.0861211529045703E-5	0.0	8.688969223236563E-5	0.0
8	0.0	3.2583634587137114E-5	0.0	1.0861211529045704E-4	0.0
9	0.0	8.688969223236563E-5	0.0	2.3894665363900547E-4	0.0
10-11	0.0	8.688969223236563E-5	0.0	2.5523847093257405E-4	0.0
12-13	0.0	8.688969223236563E-5	0.0	2.715302882261426E-4	0.0
14-15	0.0	1.1947332681950273E-4	0.0	2.823914997551883E-4	5.430605764522852E-6
16-17	0.0	1.1947332681950273E-4	0.0	3.0954452857780254E-4	1.0861211529045703E-5
18-19	0.0	1.3033453834854843E-4	0.0	3.964342208101682E-4	1.0861211529045703E-5
20-21	0.0	1.4119574987759415E-4	0.0	5.48491182216808E-4	1.0861211529045703E-5
22-23	5.430605764522852E-6	1.6291817293568556E-4	0.0	9.775090376141133E-4	1.0861211529045703E-5
24-25	1.0861211529045703E-5	1.9550180752282264E-4	0.0	0.0023025768441576893	1.0861211529045703E-5
26-27	1.0861211529045703E-5	2.0636301905186836E-4	0.0	0.005691274841219949	1.0861211529045703E-5
28-29	1.0861211529045703E-5	2.0636301905186836E-4	0.0	0.026783747630626704	1.0861211529045703E-5
30-31	1.0861211529045703E-5	2.3894665363900547E-4	0.0	0.03698242525640062	1.0861211529045703E-5
32-33	1.0861211529045703E-5	2.606690766970969E-4	0.0	0.04093590625297326	1.0861211529045703E-5
34-35	3.2583634587137114E-5	2.8782210551971116E-4	0.0	0.045709408719988846	2.715302882261426E-5
36-37	3.2583634587137114E-5	3.149751343423254E-4	0.0	0.052160968368241994	3.801424035165997E-5
38-39	3.2583634587137114E-5	3.366975574004168E-4	0.0	0.06449930466523791	4.3444846116182814E-5
40-41	3.2583634587137114E-5	3.5841998045850823E-4	0.0	0.08918140786499427	4.3444846116182814E-5
42-43	3.2583634587137114E-5	3.6928119198755395E-4	0.0	0.10060740239355034	4.3444846116182814E-5
44-45	3.2583634587137114E-5	4.181566438682596E-4	0.0	0.11569905581315935	4.3444846116182814E-5
46-47	3.2583634587137114E-5	4.5617088421991955E-4	0.0	0.13301725759622274	4.3444846116182814E-5
48-49	3.2583634587137114E-5	4.616014899844424E-4	0.0	0.14860309614040332	5.430605764522852E-5
50-51	3.2583634587137114E-5	5.376299706877623E-4	0.0	0.16216331873441686	5.430605764522852E-5
52-53	3.2583634587137114E-5	5.430605764522852E-4	0.0	0.18152342828494084	5.430605764522852E-5
54-55	3.2583634587137114E-5	5.48491182216808E-4	0.0	0.22064008160679893	5.430605764522852E-5
56-57	3.2583634587137114E-5	5.647829995103766E-4	0.0	0.2603866851973417	5.430605764522852E-5
58-59	3.801424035165997E-5	5.702136052748994E-4	0.0	0.29177558651628377	5.430605764522852E-5
60-61	4.3444846116182814E-5	5.86505422568468E-4	0.0	0.3119774399603088	5.430605764522852E-5
62-63	4.3444846116182814E-5	5.973666340975136E-4	0.0	0.3287905954072715	5.430605764522852E-5
64-65	4.3444846116182814E-5	6.733951148008336E-4	0.0	0.3374361197843919	5.430605764522852E-5
66-67	4.3444846116182814E-5	6.733951148008336E-4	0.0	0.3431545476544345	5.430605764522852E-5
68-69	4.3444846116182814E-5	7.059787493879707E-4	0.0	0.3487154879573059	5.430605764522852E-5
70-71	4.3444846116182814E-5	7.277011724460622E-4	0.0	0.353972314337364	5.430605764522852E-5
72-73	5.430605764522852E-5	7.277011724460622E-4	0.0	0.3584145498527437	5.430605764522852E-5
74-75	5.430605764522852E-5	7.494235955041535E-4	0.0	0.3618955681478029	5.430605764522852E-5
76-77	5.430605764522852E-5	7.765766243267678E-4	0.0	0.36484438707793876	5.430605764522852E-5
78-79	5.430605764522852E-5	7.820072300912907E-4	0.0	0.3675922735947873	5.430605764522852E-5
80-81	5.430605764522852E-5	7.820072300912907E-4	0.0	0.3688467435263921	5.9736663409751374E-5
82-83	5.430605764522852E-5	7.928684416203364E-4	0.0	0.3692431777472023	6.516726917427423E-5
84-85	5.430605764522852E-5	7.928684416203364E-4	0.0	0.36950384682389936	6.516726917427423E-5
86-87	5.430605764522852E-5	7.928684416203364E-4	0.0	0.36962332015071886	6.516726917427423E-5
88-89	5.430605764522852E-5	8.254520762074734E-4	0.0	0.3696396119680124	6.516726917427423E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13700	0.0	50.860638	1
GTATCAA	24730	0.0	36.869232	1
CTTATAC	10960	0.0	29.268124	1
TCAACGC	31225	0.0	28.736317	4
ATCAACG	31370	0.0	28.562704	3
CAACGCA	31570	0.0	28.355303	5
TATCAAC	32370	0.0	27.891684	2
AACGCAG	32630	0.0	27.460209	6
TATACAC	14555	0.0	24.314764	3
ACGCAGA	37275	0.0	24.026875	7
CGCAGAG	37560	0.0	23.889809	8
TTATACA	15025	0.0	22.000048	2
GCAGAGT	43325	0.0	20.652431	9
GTACATG	28775	0.0	20.05132	1
TACATGG	28245	0.0	19.81083	2
GAGTACT	26505	0.0	19.411781	12-13
ACATCTC	16675	0.0	19.312908	8
AGAGTAC	39915	0.0	19.30341	10-11
TACACAT	18365	0.0	19.131935	5
ACACATC	16855	0.0	18.95542	6
>>END_MODULE