FastQCFastQC Report
Fri 27 May 2016
SRR2139047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9143576
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132900.051.9747921
GTATCAA253750.037.795521
ATCAACG314100.029.80133
TCAACGC314500.029.7638854
TATCAAC325450.029.0513362
CAACGCA322350.029.0262135
AACGCAG328050.028.5736316
ACGCAGA373700.025.049097
CGCAGAG381450.024.5400288
CTTATAC101850.024.4268111
GCAGAGT434000.021.4418379
TACATGG307950.020.9001392
GTACATG311900.020.8804651
TATACAC141250.020.2565753
ACATGGG310600.020.1187463
GAGTACT259900.019.55224812-13
AGAGTAC405800.019.25472610-11
CAGAGTA429250.018.7220710-11
CATGGGG213300.018.270634
GTGGTAT65900.018.2318691
Produced by FastQC (version 0.11.5)