##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9143576
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67744731382995	34.0	31.0	34.0	31.0	34.0
2	32.81951087845718	34.0	31.0	34.0	31.0	34.0
3	32.86620890994946	34.0	31.0	34.0	31.0	34.0
4	36.32864592583908	37.0	37.0	37.0	35.0	37.0
5	36.26775618204519	37.0	37.0	37.0	35.0	37.0
6	36.30403837623267	37.0	37.0	37.0	35.0	37.0
7	36.25795706187601	37.0	37.0	37.0	35.0	37.0
8	36.24007051508075	37.0	37.0	37.0	35.0	37.0
9	37.91922547589696	39.0	38.0	39.0	35.0	39.0
10-11	37.89195928376381	39.0	38.0	39.0	35.0	39.0
12-13	37.8657316349752	39.0	38.0	39.0	35.0	39.0
14-15	39.27995512915297	41.0	39.0	41.0	36.0	41.0
16-17	39.266633207839035	41.0	39.0	41.0	36.0	41.0
18-19	39.30310520741557	41.0	39.0	41.0	36.0	41.0
20-21	39.224215405255784	41.0	39.0	41.0	36.0	41.0
22-23	39.21710632007567	40.5	39.0	41.0	36.0	41.0
24-25	39.123031294076824	40.0	39.0	41.0	36.0	41.0
26-27	38.98440080562892	40.0	39.0	41.0	35.0	41.0
28-29	38.85376957548901	40.0	38.5	41.0	35.0	41.0
30-31	38.730275933941854	40.0	38.0	41.0	35.0	41.0
32-33	38.63851157522873	40.0	38.0	41.0	35.0	41.0
34-35	38.49671084053304	40.0	38.0	41.0	34.5	41.0
36-37	38.630626725243246	40.0	38.0	41.0	35.0	41.0
38-39	38.690253858482336	40.0	38.0	41.0	35.0	41.0
40-41	38.679045171890465	40.0	38.0	41.0	35.0	41.0
42-43	38.64881189438931	40.0	38.0	41.0	35.0	41.0
44-45	38.57947442613554	40.0	38.0	41.0	34.5	41.0
46-47	38.51586423450813	40.0	38.0	41.0	34.0	41.0
48-49	38.435728903958406	40.0	38.0	41.0	34.0	41.0
50-51	38.3357140454943	40.0	38.0	41.0	34.0	41.0
52-53	38.22981595427977	40.0	37.5	41.0	34.0	41.0
54-55	38.09823593640707	40.0	37.0	41.0	33.0	41.0
56-57	37.9440180909583	40.0	37.0	41.0	33.0	41.0
58-59	37.75897126710074	39.5	36.5	41.0	33.0	41.0
60-61	37.53532140220911	39.0	36.0	41.0	33.0	41.0
62-63	37.34350435475248	39.0	35.5	40.5	33.0	41.0
64-65	37.11449486123365	38.5	35.0	40.0	32.0	41.0
66-67	36.84361199136248	38.0	35.0	40.0	32.0	41.0
68-69	36.49851171777938	37.5	35.0	40.0	31.5	41.0
70-71	36.22681719921101	37.0	35.0	39.5	31.0	41.0
72-73	35.90286091660326	36.5	35.0	39.0	31.0	40.5
74-75	35.57242427865981	36.0	34.0	39.0	31.0	40.0
76-77	34.543624808304784	35.0	33.5	37.0	29.5	39.0
78-79	34.83298434432176	35.0	34.0	37.0	30.5	39.0
80-81	34.66547298704918	35.0	34.0	37.0	31.0	39.0
82-83	34.386284907347886	35.0	34.0	36.0	30.0	38.5
84-85	34.114609578380716	35.0	34.0	36.0	30.0	37.0
86-87	33.88187838883704	35.0	34.0	35.5	30.0	37.0
88-89	33.692136918471576	35.0	33.0	35.0	30.0	36.5
90-91	33.50219236909065	35.0	33.0	35.0	30.0	36.0
92-93	33.360462565634634	35.0	33.0	35.0	30.0	36.0
94-95	33.267488505571364	34.0	33.0	35.0	29.5	35.5
96-97	33.24400375128061	34.0	33.0	35.0	30.0	35.0
98-99	33.25162429850374	34.0	33.0	35.0	30.0	35.0
100-101	32.25496192018387	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	0.0
19	9.0
20	46.0
21	238.0
22	1096.0
23	3672.0
24	9401.0
25	19323.0
26	34411.0
27	54595.0
28	78272.0
29	105153.0
30	135544.0
31	170624.0
32	215083.0
33	280872.0
34	394702.0
35	575958.0
36	994613.0
37	2048051.0
38	2740694.0
39	1231932.0
40	49285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.80322945929021	21.658215923007788	15.299203571819946	24.23935104588206
2	13.366638823376158	22.28901160547869	41.95790650101276	22.38644307013239
3	16.234166202406193	27.998351629242567	32.089082452878934	23.67839971547231
4	11.445316678139204	19.580811616989156	39.87423298591995	29.09963871895169
5	12.342307285969358	38.53634257239018	36.22375670607577	12.89759343556469
6	27.932447421544353	39.51427527135451	19.498185282910164	13.055092024190978
7	24.770215672777407	33.67982804098266	24.216766879121884	17.33318940711805
8	23.78373625373705	36.95259928938087	21.959296887782198	17.30436756909988
9	25.363665156827043	16.758301128573766	21.672352261303455	36.20568145329573
10-11	23.247955284565517	27.21904537858248	29.82586719027306	19.70713214657895
12-13	24.434143709200864	25.089407032872042	29.645272265468126	20.831176992458968
14-15	21.661450213955128	26.241547826402694	27.409152462961146	24.687849496681032
16-17	20.959660489038967	29.469816523850344	27.939402228106303	21.631120759004386
18-19	21.088146594740024	28.378921811217168	29.92067107231034	20.612260521732473
20-21	21.964789402478544	27.544013246901823	29.71438555352735	20.776811797092286
22-23	21.580922559945275	27.739171994221046	29.56048216433946	21.119423281494214
24-25	21.536543158077848	27.81804471341137	29.671135783228653	20.974276345282128
26-27	21.472317091885376	27.958287972122363	29.608875991333555	20.960518944658702
28-29	21.324320723082753	27.89882140507567	29.6478418606889	21.129016011152675
30-31	21.442398602596036	27.911484625136136	29.72310915123414	20.923007621033687
32-33	21.403534016980924	28.01610982467747	29.56968794795457	21.01066821038703
34-35	21.56814342640488	27.902861207605024	29.624795751351872	20.904199614638223
36-37	21.316755013547102	28.06186567522871	29.46679700504103	21.15458230618316
38-39	21.2780827965859	27.874065087630406	29.60495492816657	21.24289718761712
40-41	21.35486884574084	27.80201769733529	29.513254463165943	21.32985899375793
42-43	21.457576464497148	27.864860995590245	29.321926850474426	21.355635689438177
44-45	21.408528565230522	28.0925627208096	29.06557325910996	21.43333545484992
46-47	21.64097787561051	27.929180472515146	28.63499088207678	21.79485076979756
48-49	21.598590770761934	28.08496562538923	28.55222931069637	21.764214293152467
50-51	21.521332464985225	28.260786765536533	28.48607023600282	21.731810533475425
52-53	21.610813492593365	28.266168875215648	28.48786054717892	21.635157085012075
54-55	21.6205426652844	28.240644550638315	28.333202355307858	21.805610428769427
56-57	21.77421043039485	28.395669175552275	28.11180351819913	21.71831687585375
58-59	21.51460190252619	28.544688394385638	28.39201450977331	21.548695193314867
60-61	21.800181296240453	28.616446562948493	28.060846436428434	21.522525704382616
62-63	21.61225712820314	28.79732875158199	28.030432798746023	21.55998132146884
64-65	21.606019214264126	28.631404540571058	28.10081428037262	21.66176196479219
66-67	21.671924454122795	28.562958868362948	27.955569731770467	21.80954694574379
68-69	21.719301096240223	28.433862172151798	28.022080114160296	21.824756617447683
70-71	21.713948559167342	28.242830896146963	28.11405303174753	21.92916751293816
72-73	21.757675414864185	28.21100636976994	28.05410469366509	21.977213521700786
74-75	21.688859527145794	28.30004508799951	28.05049301340683	21.96060237144787
76-77	21.78191531570532	28.256073908078218	28.016842489356414	21.94516828686005
78-79	21.766008811274304	28.221530794017852	27.966953375891134	22.045507018816714
80-81	21.812940852631666	28.22499041415548	27.931791973584552	22.030276759628297
82-83	21.841079967173272	28.17535337542097	28.061680524384485	21.921886133021278
84-85	21.934721131968672	28.17068971678423	27.973604691589067	21.920984459658033
86-87	21.803192811734203	28.208518730857758	28.04874989500482	21.939538562403214
88-89	21.84966786835033	28.06675974917799	28.087354129972297	21.99621825249938
90-91	21.908113565287344	28.08453675199264	28.076966542639113	21.930383140080902
92-93	21.99435815169292	28.00980278481867	28.08643153094577	21.909407532542634
94-95	21.762315099326084	28.089245444132537	28.187891942010385	21.960547514530994
96-97	21.758301010227584	28.133499647815796	28.188664414966023	21.9195349269906
98-99	21.978692597306747	28.681826790057112	28.24374164358164	21.095738969054505
100-101	22.512588236363616	31.372362463185844	25.46813517582755	20.646914124622988
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2856.0
1	2539.5
2	3328.5
3	4696.5
4	6350.0
5	9592.0
6	12161.0
7	14482.5
8	16188.0
9	16125.0
10	14707.0
11	13336.5
12	14802.5
13	19889.0
14	31615.0
15	47825.5
16	60608.0
17	66811.5
18	66787.5
19	62397.5
20	56543.0
21	51341.5
22	49513.0
23	53897.5
24	63962.0
25	79370.0
26	99122.5
27	120016.0
28	142480.0
29	165799.5
30	186887.5
31	207964.5
32	230001.5
33	252296.0
34	271022.0
35	285377.0
36	303840.5
37	321417.5
38	331333.5
39	338758.5
40	347690.5
41	356285.5
42	358435.5
43	360127.5
44	364300.5
45	369814.0
46	375721.0
47	377770.5
48	370273.5
49	358498.5
50	348271.5
51	331660.0
52	310558.5
53	288634.5
54	268566.5
55	248545.5
56	226810.0
57	203129.0
58	178418.5
59	153691.5
60	127971.0
61	103264.0
62	81421.5
63	62871.0
64	47246.0
65	35334.5
66	26662.5
67	20404.0
68	15553.5
69	11096.5
70	7698.5
71	5517.5
72	3901.5
73	2731.5
74	1857.5
75	1234.0
76	795.0
77	500.5
78	333.5
79	203.5
80	134.5
81	96.5
82	71.0
83	49.5
84	34.0
85	22.0
86	15.5
87	13.5
88	9.5
89	4.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.1873280213343227E-5
2	0.008082177038830322
3	2.1873280213343227E-5
4	2.1873280213343227E-5
5	2.1873280213343227E-5
6	2.1873280213343227E-5
7	2.1873280213343227E-5
8	0.0
9	0.0
10-11	7.272865670936622E-4
12-13	0.0
14-15	7.65564807467013E-4
16-17	7.819697676270203E-4
18-19	1.0936640106671613E-5
20-21	5.085681984212184E-4
22-23	0.001148471119697543
24-25	0.0042228674068317575
26-27	0.016771621999705937
28-29	0.3218924182061161
30-31	0.30951337844196725
32-33	0.3186418098942734
34-35	0.18467616845259205
36-37	0.04574865293324171
38-39	0.035959287037982
40-41	0.031213245603034793
42-43	0.027176064508574748
44-45	0.01498358403559958
46-47	0.007691032071834702
48-49	0.005833528027331246
50-51	0.002289928224583916
52-53	0.0018396818181278687
54-55	0.0014473161376227796
56-57	5.912073352662596E-4
58-59	0.0027644090253692373
60-61	0.002557385800889933
62-63	3.847901633732146E-4
64-65	2.0682560859532133E-4
66-67	0.0077422497355787295
68-69	0.00668222606669118
70-71	0.009192949059862347
72-73	0.009565542600990684
74-75	0.007334762522704094
76-77	0.004580902437780242
78-79	3.907542952486905E-4
80-81	5.176908237326095E-4
82-83	5.993260268831478E-4
84-85	0.0
86-87	0.0020293957981360002
88-89	0.0015836093295704303
90-91	9.760919649266297E-4
92-93	0.011622852523905361
94-95	9.692413494463992E-5
96-97	2.27828006258891E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	154.0
20-21	577.0
22-23	1283.0
24-25	3193.0
26-27	4313.0
28-29	7921.0
30-31	21720.0
32-33	28455.0
34-35	39981.0
36-37	54416.0
38-39	62407.0
40-41	65904.0
42-43	77911.0
44-45	92275.0
46-47	91284.0
48-49	79951.0
50-51	70576.0
52-53	64006.0
54-55	69638.0
56-57	76638.0
58-59	78194.0
60-61	77863.0
62-63	77427.0
64-65	82233.0
66-67	89567.0
68-69	82733.0
70-71	77415.0
72-73	73590.0
74-75	74669.0
76-77	75686.0
78-79	80567.0
80-81	82743.0
82-83	80371.0
84-85	81520.0
86-87	84890.0
88-89	91706.0
90-91	100340.0
92-93	104361.0
94-95	116664.0
96-97	171297.0
98-99	754481.0
100-101	5692656.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.97123852034754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.15670882678765	74.86599262583361
2	7.428406366257301	12.475449656141198
3	1.8363842291891426	4.6261037437273815
4	0.7086099040682305	2.380114050895759
5	0.3382068881702774	1.4199825637885433
6	0.1774018922147919	0.8937993963077567
7	0.10778540367584069	0.6335611688753173
8	0.06524219512446149	0.4382774342709768
9	0.03944248002776053	0.29808305084206116
>10	0.1397875887562822	1.801252720545337
>50	0.0016253279734646693	0.08344824218858288
>100	3.327124265135712E-4	0.045457934222203025
>500	6.618532826600659E-5	0.038477412361180505
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0936640106671613E-5	0.0	0.0	0.0
3	0.0	1.0936640106671613E-5	0.0	0.0	0.0
4	0.0	5.4683200533358065E-5	0.0	0.0	0.0
5	1.0936640106671613E-5	6.561984064002968E-5	0.0	3.280992032001484E-5	0.0
6	1.0936640106671613E-4	6.561984064002968E-5	0.0	3.280992032001484E-5	1.0936640106671613E-5
7	1.0936640106671613E-4	6.561984064002968E-5	0.0	3.280992032001484E-5	1.0936640106671613E-5
8	1.0936640106671613E-4	7.65564807467013E-5	0.0	4.374656042668645E-5	1.0936640106671613E-5
9	1.0936640106671613E-4	1.2030304117338774E-4	0.0	6.561984064002968E-5	1.0936640106671613E-5
10-11	1.1483472112005193E-4	1.2030304117338774E-4	0.0	8.202480080003711E-5	1.0936640106671613E-5
12-13	1.2030304117338774E-4	1.2030304117338774E-4	0.0	8.74931208533729E-5	1.0936640106671613E-5
14-15	1.2030304117338774E-4	1.9139120186675322E-4	0.0	1.0936640106671613E-4	2.7341600266679036E-5
16-17	2.0779616202676063E-4	1.9685952192008904E-4	0.0	1.2577136122672355E-4	3.280992032001484E-5
18-19	2.0779616202676063E-4	2.0779616202676066E-4	0.0	1.6404960160007421E-4	4.374656042668645E-5
20-21	2.0779616202676063E-4	2.1873280213343226E-4	0.0	2.6247936256011873E-4	4.374656042668645E-5
22-23	2.1326448208009645E-4	2.4060608234677548E-4	0.0	6.890083267203116E-4	4.921488048002226E-5
24-25	2.1873280213343226E-4	2.6247936256011873E-4	0.0	0.002012341779627577	7.108816069336548E-5
26-27	2.1873280213343226E-4	2.6247936256011873E-4	0.0	0.005621433014829209	8.202480080003711E-5
28-29	2.2966944224010388E-4	2.6247936256011873E-4	0.0	0.02556986456939823	8.74931208533729E-5
30-31	2.2966944224010388E-4	2.734160026667903E-4	0.0	0.035719066588389484	9.842976096004452E-5
32-33	2.2966944224010388E-4	2.734160026667903E-4	0.0	0.03970000358721795	1.0389808101338033E-4
34-35	2.2966944224010388E-4	3.3356752325348416E-4	0.0	0.04460508667506018	1.0936640106671613E-4
36-37	2.2966944224010388E-4	3.9371904384017807E-4	0.0	0.05212949506845024	1.0936640106671613E-4
38-39	2.2966944224010388E-4	3.9371904384017807E-4	0.0	0.0644933667090425	1.1483472112005193E-4
40-41	2.2966944224010388E-4	3.9371904384017807E-4	0.0	0.0903639888813742	1.4217632138673096E-4
42-43	2.2966944224010388E-4	3.991873638935139E-4	0.0	0.10166700643161931	1.531129614934026E-4
44-45	2.2966944224010388E-4	4.265289641601929E-4	0.0	0.11652990033658603	1.6951792165341E-4
46-47	2.2966944224010388E-4	4.53870564426872E-4	0.0	0.13356918562278042	1.9685952192008904E-4
48-49	2.2966944224010388E-4	4.5933888448020777E-4	0.0	0.1490828096140941	2.1873280213343226E-4
50-51	2.351377622934397E-4	5.085537649602301E-4	0.0	0.1642355244818876	2.1873280213343226E-4
52-53	2.4060608234677548E-4	5.140220850135658E-4	0.0	0.18403084307496323	2.4607440240011127E-4
54-55	2.4060608234677548E-4	5.140220850135658E-4	0.0	0.22399879434479464	2.734160026667903E-4
56-57	2.4060608234677548E-4	5.140220850135658E-4	0.0	0.26507134626539985	2.734160026667903E-4
58-59	2.4060608234677548E-4	5.140220850135658E-4	0.0	0.2973070929798145	2.734160026667903E-4
60-61	2.4607440240011127E-4	5.249587251202374E-4	0.0	0.31761643365790365	2.734160026667903E-4
62-63	2.6247936256011873E-4	5.35895365226909E-4	0.0	0.33573844631465855	2.734160026667903E-4
64-65	2.679476826134545E-4	6.343251261869535E-4	0.0	0.34450963168020915	2.788843227201261E-4
66-67	2.734160026667903E-4	6.452617662936252E-4	0.0	0.3503607341372785	2.9528928288013355E-4
68-69	2.734160026667903E-4	6.50730086346961E-4	0.0	0.3560532553128011	3.062259229868052E-4
70-71	2.734160026667903E-4	6.561984064002968E-4	0.0	0.3618059280089103	3.062259229868052E-4
72-73	2.8435264277346193E-4	6.561984064002968E-4	0.0	0.36590716804891216	3.280992032001484E-4
74-75	2.8435264277346193E-4	6.999449668269833E-4	0.0	0.3693904879228871	3.280992032001484E-4
76-77	2.8982096282679777E-4	7.546281673603413E-4	0.0	0.37261132843430184	3.280992032001484E-4
78-79	2.9528928288013355E-4	7.765014475736845E-4	0.0	0.37547672814224986	3.280992032001484E-4
80-81	2.9528928288013355E-4	7.765014475736845E-4	0.0	0.37664148031361033	3.3356752325348416E-4
82-83	3.0075760293346934E-4	7.874380876803561E-4	0.0	0.37708441423793054	3.4450416336015584E-4
84-85	3.062259229868052E-4	7.983747277870277E-4	0.0	0.377368766880704	3.499724834134916E-4
86-87	3.062259229868052E-4	8.20248008000371E-4	0.0	0.3774453233614507	3.499724834134916E-4
88-89	3.062259229868052E-4	9.241460890137513E-4	0.0	0.3774781332817707	3.554408034668274E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13290	0.0	51.974792	1
GTATCAA	25375	0.0	37.79552	1
ATCAACG	31410	0.0	29.8013	3
TCAACGC	31450	0.0	29.763885	4
TATCAAC	32545	0.0	29.051336	2
CAACGCA	32235	0.0	29.026213	5
AACGCAG	32805	0.0	28.573631	6
ACGCAGA	37370	0.0	25.04909	7
CGCAGAG	38145	0.0	24.540028	8
CTTATAC	10185	0.0	24.426811	1
GCAGAGT	43400	0.0	21.441837	9
TACATGG	30795	0.0	20.900139	2
GTACATG	31190	0.0	20.880465	1
TATACAC	14125	0.0	20.256575	3
ACATGGG	31060	0.0	20.118746	3
GAGTACT	25990	0.0	19.552248	12-13
AGAGTAC	40580	0.0	19.254726	10-11
CAGAGTA	42925	0.0	18.72207	10-11
CATGGGG	21330	0.0	18.27063	4
GTGGTAT	6590	0.0	18.231869	1
>>END_MODULE