FastQCFastQC Report
Fri 27 May 2016
SRR2139046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8283628
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117350.053.647091
GTATCAA225300.038.8957561
TCAACGC288600.029.7691044
ATCAACG289550.029.6710723
TATCAAC294750.029.3827652
CAACGCA294500.029.1874075
AACGCAG301900.028.6136346
CGCAGAG351550.024.4386718
ACGCAGA354600.024.2405267
CTTATAC83650.022.291641
GCAGAGT404350.021.1208119
GTACATG280150.020.7925591
TACATGG277700.020.5909792
GAGTACT232600.020.37111512-13
TATACAC109950.020.3415163
ACATGGG280250.020.1116033
AGAGTAC365550.019.71256410-11
GTGGTAT52000.019.1634651
CAGAGTA393800.018.67310110-11
AGTACTT254750.018.49916612-13
Produced by FastQC (version 0.11.5)