##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139046_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8283628
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.681942260082174	34.0	31.0	34.0	31.0	34.0
2	32.82082041829981	34.0	31.0	34.0	31.0	34.0
3	32.86643400693512	34.0	31.0	34.0	31.0	34.0
4	36.33214492490488	37.0	37.0	37.0	35.0	37.0
5	36.26927766432775	37.0	37.0	37.0	35.0	37.0
6	36.304057352647895	37.0	37.0	37.0	35.0	37.0
7	36.25517164701264	37.0	37.0	37.0	35.0	37.0
8	36.23781584590713	37.0	37.0	37.0	35.0	37.0
9	37.91720717057792	39.0	38.0	39.0	35.0	39.0
10-11	37.8875359926834	39.0	38.0	39.0	35.0	39.0
12-13	37.86073155385539	39.0	38.0	39.0	35.0	39.0
14-15	39.27280733755789	41.0	39.0	41.0	36.0	41.0
16-17	39.258393061590894	41.0	39.0	41.0	36.0	41.0
18-19	39.28903778634193	41.0	39.0	41.0	36.0	41.0
20-21	39.20644427758159	41.0	39.0	41.0	36.0	41.0
22-23	39.19898136651725	40.0	39.0	41.0	36.0	41.0
24-25	39.101302768401354	40.0	39.0	41.0	35.5	41.0
26-27	38.95907852397501	40.0	39.0	41.0	35.0	41.0
28-29	38.826078768287765	40.0	38.0	41.0	35.0	41.0
30-31	38.70164783537306	40.0	38.0	41.0	35.0	41.0
32-33	38.60555403869256	40.0	38.0	41.0	35.0	41.0
34-35	38.46196009376918	40.0	38.0	41.0	34.5	41.0
36-37	38.60418532335008	40.0	38.0	41.0	35.0	41.0
38-39	38.66048663535405	40.0	38.0	41.0	35.0	41.0
40-41	38.64214720124819	40.0	38.0	41.0	35.0	41.0
42-43	38.607483658986915	40.0	38.0	41.0	35.0	41.0
44-45	38.531316467787164	40.0	38.0	41.0	34.0	41.0
46-47	38.46101911945288	40.0	38.0	41.0	34.0	41.0
48-49	38.36577598317385	40.0	38.0	41.0	34.0	41.0
50-51	38.253682605160506	40.0	38.0	41.0	34.0	41.0
52-53	38.135032525543934	40.0	37.0	41.0	33.5	41.0
54-55	37.98538928318277	40.0	37.0	41.0	33.0	41.0
56-57	37.81473566954359	40.0	36.5	41.0	33.0	41.0
58-59	37.608914676670466	39.0	36.0	41.0	33.0	41.0
60-61	37.36555542531737	39.0	35.5	40.5	33.0	41.0
62-63	37.14827462321523	39.0	35.0	40.0	32.0	41.0
64-65	36.90092242033201	38.0	35.0	40.0	32.0	41.0
66-67	36.61375165880985	37.5	35.0	40.0	32.0	41.0
68-69	36.250877187114725	37.0	35.0	40.0	31.0	41.0
70-71	35.97206524224918	36.5	35.0	39.0	31.0	41.0
72-73	35.64709707377672	36.0	34.0	39.0	31.0	40.0
74-75	35.322612262001	35.5	34.0	38.5	31.0	40.0
76-77	34.31385137178063	35.0	33.0	37.0	29.5	39.0
78-79	34.61266735128148	35.0	34.0	37.0	30.0	39.0
80-81	34.466018875862474	35.0	34.0	36.5	30.0	39.0
82-83	34.20404111298035	35.0	34.0	36.0	30.0	37.5
84-85	33.95568404456999	35.0	34.0	36.0	30.0	37.0
86-87	33.74765661176228	35.0	33.0	35.0	30.0	37.0
88-89	33.575731267491484	35.0	33.0	35.0	30.0	36.0
90-91	33.39897678039805	35.0	33.0	35.0	29.5	36.0
92-93	33.27084761798493	34.5	33.0	35.0	29.0	36.0
94-95	33.186350325118454	34.0	33.0	35.0	29.0	35.0
96-97	33.17143219810568	34.0	33.0	35.0	29.0	35.0
98-99	33.18457613781838	34.0	33.0	35.0	29.5	35.0
100-101	32.184571633843674	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	3.0
19	9.0
20	38.0
21	183.0
22	931.0
23	3237.0
24	8545.0
25	17647.0
26	31495.0
27	50373.0
28	72767.0
29	97854.0
30	125933.0
31	160228.0
32	202287.0
33	265561.0
34	376416.0
35	554997.0
36	976242.0
37	1973450.0
38	2355174.0
39	972304.0
40	37951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.065991262040164	21.223130882160675	15.147821117488752	24.563056738310404
2	13.735393353993421	21.799311091603858	41.213037514733564	23.252258039669158
3	16.524790408839028	27.485592965496874	31.62495124418325	24.36466538148084
4	11.943392905474004	18.98095109557095	39.01442435957394	30.061231639381113
5	12.803268423360926	38.173169796274024	35.75775442327377	13.265807357091283
6	28.94967385663309	38.632521720256115	18.971931015242475	13.445873407868318
7	25.407855761733355	33.290260413705255	23.698012959781988	17.603870864779402
8	24.59097632100331	36.25425960702243	21.4244652222432	17.73029884973106
9	25.88968263664182	16.52751668713274	21.22221084771069	36.36058982851475
10-11	23.914820701529123	26.83114462111831	29.135811381625444	20.118223295727127
12-13	24.986361650518546	24.538387592777898	29.09895025452015	21.376300502183405
14-15	22.222251061137964	25.624996981973936	26.924480537353507	25.228271419534597
16-17	21.52681029124724	28.85842548318728	27.431141199074528	22.183623026490952
18-19	21.679710972034897	27.87491349653277	29.36443001571473	21.080945515717602
20-21	22.574673398574344	26.940165908960857	29.180259214340886	21.304901478123917
22-23	22.282819030150446	26.989032267528568	29.049263369676325	21.67888533264466
24-25	22.135470901128393	27.102941772306906	29.173679879171573	21.587907447393132
26-27	22.061938255065993	27.380979926326553	28.962128706515976	21.594953112091478
28-29	21.979210754644306	27.3137234252752	29.032753650128356	21.67431216995213
30-31	22.07248336779321	27.263302639703923	29.090469226882032	21.573744765620837
32-33	22.026860319717848	27.37695418688449	28.950798953905547	21.645386539492108
34-35	22.174178958171883	27.222884782841643	29.018869019384685	21.58406723960179
36-37	22.013775115670413	27.33887832388719	28.905056021204263	21.742290539238134
38-39	21.969172386357837	27.238703417796	28.973732767912423	21.818391427933737
40-41	22.06705397058292	27.26349289324045	28.81949652470968	21.849956611466947
42-43	22.06444937825178	27.30375018244188	28.739927681347226	21.891872757959106
44-45	22.126529144818	27.513746956672016	28.417826513912853	21.941897384597134
46-47	22.32663843900462	27.26462728348608	27.982131511476116	22.426602766033184
48-49	22.2514793806059	27.515382981061386	27.908328729134773	22.324808909197937
50-51	22.130776398724162	27.621958496337506	27.897946641990153	22.349318462948183
52-53	22.24735693160817	27.61322228124906	27.79058284629678	22.34883794084599
54-55	22.28889987040384	27.58271085678424	27.672584659587397	22.45580461322453
56-57	22.436932901856693	27.700178827013787	27.52385737471366	22.339030896415856
58-59	22.17741782253151	27.98499071870238	27.75316995164978	22.08442150711633
60-61	22.47780051842948	27.95863753699465	27.43891696278976	22.12464498178611
62-63	22.330270008685016	28.07095328641865	27.363034661723567	22.235742043172767
64-65	22.267074072046427	27.952740063505964	27.494148965290705	22.2860368991569
66-67	22.44061602696654	27.958779107247906	27.27509802147539	22.325506844310166
68-69	22.350147788671766	27.73430687064068	27.517521758455487	22.398023582232067
70-71	22.378365270402398	27.55336179859873	27.553382946641374	22.5148899843575
72-73	22.463037098925916	27.55390197788088	27.484914901948287	22.498146021244917
74-75	22.306791854932023	27.67085036253496	27.48244619700868	22.539911585524333
76-77	22.545667709348805	27.612249210596573	27.315090599134955	22.52699248091967
78-79	22.427502910126655	27.652517341867267	27.3142256356002	22.605754112405883
80-81	22.462655828032737	27.591900061889145	27.331517662062783	22.613926448015334
82-83	22.459314660215057	27.49709543853667	27.465597448363955	22.577992452884317
84-85	22.571662236514413	27.52733479191371	27.41595558482313	22.485047386748743
86-87	22.34675363503131	27.545643927409973	27.442060123181605	22.665542314377113
88-89	22.433266191129896	27.49384049362839	27.473626024129654	22.599267291112067
90-91	22.48065828457893	27.56422788284929	27.50995002777625	22.44516380479553
92-93	22.578964427138892	27.51587131712998	27.448899049675816	22.45626520605531
94-95	22.397534790810642	27.51151681500487	27.482648782497776	22.608299611686714
96-97	22.331021142226888	27.50900498612833	27.643180058241178	22.516793813403606
98-99	22.58139450127507	28.120404970576484	27.636825223601345	21.661375304547096
100-101	23.175599036784007	30.776418127069626	24.77931227659776	21.26867055954861
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2853.0
1	2475.5
2	3128.5
3	4327.5
4	5674.5
5	8721.0
6	11260.5
7	13668.0
8	15609.0
9	15424.0
10	13705.0
11	12084.0
12	13020.0
13	17390.0
14	28268.5
15	43204.0
16	54663.0
17	60119.0
18	59883.5
19	55025.0
20	47921.5
21	41774.0
22	38760.0
23	41313.5
24	49700.0
25	62894.5
26	79267.5
27	95953.0
28	113000.5
29	132112.5
30	146802.0
31	159928.5
32	178657.5
33	196912.5
34	213214.0
35	228596.0
36	246955.0
37	261196.5
38	266601.0
39	270306.0
40	279388.0
41	289587.5
42	293827.0
43	301127.0
44	311637.5
45	320991.0
46	332333.5
47	338441.0
48	335145.0
49	332248.0
50	328985.0
51	320230.5
52	306899.0
53	293786.0
54	281580.0
55	263961.0
56	243958.0
57	222306.5
58	198888.5
59	175031.5
60	147701.0
61	120470.0
62	94745.5
63	71669.0
64	53310.0
65	40499.5
66	31395.0
67	23805.0
68	17834.5
69	12748.0
70	8957.5
71	6474.5
72	4531.5
73	3050.5
74	2026.0
75	1356.0
76	877.5
77	550.0
78	343.5
79	206.0
80	135.5
81	94.5
82	72.0
83	48.0
84	26.0
85	15.0
86	9.0
87	5.0
88	4.0
89	3.5
90	3.5
91	3.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.207200516488669E-5
2	0.008076171455309195
3	1.207200516488669E-5
4	2.414401032977338E-5
5	1.207200516488669E-5
6	1.207200516488669E-5
7	1.207200516488669E-5
8	0.0
9	0.0
10-11	7.967523408825215E-4
12-13	1.207200516488669E-5
14-15	8.208963512122949E-4
16-17	7.786443331351914E-4
18-19	6.036002582443345E-6
20-21	4.768586243956647E-4
22-23	0.001177150672388464
24-25	0.004655298399797027
26-27	0.017293202472776947
28-29	0.32338378661069533
30-31	0.31108605669516914
32-33	0.3195827539209156
34-35	0.1845843839749434
36-37	0.04413160555632924
38-39	0.033369353877971915
40-41	0.030088181134809405
42-43	0.02608035990647456
44-45	0.013645926241600149
46-47	0.007015996408317785
48-49	0.0050452375745959534
50-51	0.0020428303958164905
52-53	0.0016216378855248696
54-55	0.0013380536016401913
56-57	4.297118649726323E-4
58-59	0.002420019037483095
60-61	0.0022593697272967634
62-63	3.119948946783234E-4
64-65	1.2317952635829723E-4
66-67	0.007630049318178378
68-69	0.006807017832920596
70-71	0.008860244758446255
72-73	0.009546438872416184
74-75	0.007157711522400729
76-77	0.004550838078862256
78-79	3.2192372251521346E-4
80-81	5.692113296118741E-4
82-83	7.993970603704661E-4
84-85	0.0
86-87	0.0021981784580495578
88-89	0.0016909449819007116
90-91	0.0011573302954977036
92-93	0.01199726275274141
94-95	1.1273417000876508E-4
96-97	4.0989496277620876E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	150.0
20-21	514.0
22-23	1194.0
24-25	3100.0
26-27	3835.0
28-29	6817.0
30-31	18309.0
32-33	23885.0
34-35	32880.0
36-37	45143.0
38-39	51305.0
40-41	55861.0
42-43	66513.0
44-45	78994.0
46-47	78140.0
48-49	67674.0
50-51	58608.0
52-53	53552.0
54-55	57828.0
56-57	63508.0
58-59	63988.0
60-61	64370.0
62-63	64232.0
64-65	69311.0
66-67	74338.0
68-69	69415.0
70-71	65630.0
72-73	63734.0
74-75	65054.0
76-77	64562.0
78-79	69569.0
80-81	71528.0
82-83	70451.0
84-85	70681.0
86-87	73890.0
88-89	79715.0
90-91	87417.0
92-93	92902.0
94-95	104522.0
96-97	156664.0
98-99	704994.0
100-101	5268851.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.99607109388192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.2969115563638	73.28296748902173
2	7.901629843964902	13.116084645745001
3	2.1025964782024777	5.235217403599158
4	0.7903897973288077	2.6239699124392257
5	0.3663581718398599	1.5203144437922815
6	0.1860828888627638	0.9266489204045325
7	0.10688644852926495	0.6209808696775198
8	0.06882043156042	0.4569460344400203
9	0.04076831273796917	0.30452488041402914
>10	0.13715199830101812	1.6974639744484261
>50	0.002016176087228467	0.10966747112387501
>100	2.8857203849904246E-4	0.040811513738089424
>500	7.449319197462029E-5	0.038930029936942716
>1k	2.48309909968182E-5	0.025472411219118838
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.036002582443345E-5	0.0	0.0	0.0
3	0.0	6.036002582443345E-5	0.0	0.0	0.0
4	0.0	1.207200516488669E-4	0.0	0.0	0.0
5	0.0	1.3279205681375358E-4	0.0	0.0	0.0
6	1.5693606714352697E-4	1.3279205681375358E-4	0.0	0.0	0.0
7	1.5693606714352697E-4	1.3279205681375358E-4	0.0	0.0	0.0
8	1.5693606714352697E-4	1.8108007747330034E-4	0.0	0.0	0.0
9	1.5693606714352697E-4	2.8972812395728053E-4	0.0	0.0	0.0
10-11	1.75044074890857E-4	2.8972812395728053E-4	0.0	2.4144010329773378E-5	0.0
12-13	1.9315208263818705E-4	2.8972812395728053E-4	0.0	3.6216015494660066E-5	0.0
14-15	1.9315208263818705E-4	5.311682272550143E-4	0.0	3.6216015494660066E-5	0.0
16-17	2.8972812395728053E-4	5.553122375847878E-4	0.0	6.036002582443345E-5	0.0
18-19	2.8972812395728053E-4	6.458522763214379E-4	0.0	1.086480464839802E-4	0.0
20-21	2.8972812395728053E-4	7.424283176405314E-4	0.0	1.7504407489085698E-4	1.207200516488669E-5
22-23	3.138721342870539E-4	8.691843718718416E-4	0.0	4.406281885183642E-4	1.8108007747330037E-5
24-25	3.6819615752904406E-4	9.899044235207085E-4	0.0	0.0014969286404459495	2.414401032977338E-5
26-27	3.863041652763741E-4	0.001050264449345142	0.0	0.004702046011723366	2.414401032977338E-5
28-29	3.863041652763741E-4	0.001050264449345142	0.0	0.02186843735619224	2.414401032977338E-5
30-31	3.863041652763741E-4	0.0011649484984115656	0.0	0.03045766903100912	3.0180012912216725E-5
32-33	3.863041652763741E-4	0.0011830565061588957	0.0	0.033765398446188075	6.036002582443345E-5
34-35	3.9837617044126075E-4	0.0013581005810497527	0.0	0.03815960832620682	7.243203098932013E-5
36-37	4.1044817560614746E-4	0.0015331446559406095	0.0	0.04438272698870591	7.243203098932013E-5
38-39	4.1044817560614746E-4	0.0015633246688528265	0.0	0.055543295763643655	7.243203098932013E-5
40-41	4.225201807710341E-4	0.0016659367127543631	0.0	0.07848010557692837	7.243203098932013E-5
42-43	4.225201807710341E-4	0.0017625127540734568	0.0	0.08869905794900496	7.243203098932013E-5
44-45	4.345921859359208E-4	0.0020160248625360772	0.0	0.10259997189637197	7.243203098932013E-5
46-47	4.4666419110080753E-4	0.002185032934844491	0.0	0.11848069469078042	7.243203098932013E-5
48-49	4.5270019368325086E-4	0.002185032934844491	0.0	0.1324178246536421	7.243203098932013E-5
50-51	5.07024216925241E-4	0.0024566530510544415	0.0	0.1459505424434801	7.243203098932013E-5
52-53	5.311682272550143E-4	0.0024747610588017714	0.0	0.16439656633542693	7.846803357176347E-5
54-55	5.311682272550143E-4	0.0024868330639666583	0.0	0.2027553627468544	8.450403615420683E-5
56-57	5.311682272550143E-4	0.0024989050691315447	0.0	0.24083046703690703	8.450403615420683E-5
58-59	5.372042298374577E-4	0.002504941071713988	0.0	0.27123381204467417	8.450403615420683E-5
60-61	5.794562479145611E-4	0.002523049079461318	0.0	0.2895953319004668	8.450403615420683E-5
62-63	6.036002582443345E-4	0.0025532290923735348	0.0	0.3060192949272951	8.450403615420683E-5
64-65	6.036002582443345E-4	0.002800705198253712	0.0	0.31381781026381195	8.450403615420683E-5
66-67	6.156722634092211E-4	0.0028248492085834855	0.0	0.31877336838399795	9.054003873665018E-5
68-69	6.156722634092211E-4	0.002897281239572806	0.0	0.3235840624422053	9.657604131909352E-5
70-71	6.337802711565512E-4	0.002939533257649909	0.0	0.32897421274832717	9.657604131909352E-5
72-73	6.82068291816098E-4	0.0029576412653972393	0.0	0.33305455049405885	1.3279205681375358E-4
74-75	6.881042943985413E-4	0.0030602533092987756	0.0	0.3363019198834134	1.3279205681375358E-4
76-77	6.941402969809847E-4	0.003271513399684293	0.0	0.3392354171384809	1.388280593961969E-4
78-79	7.00176299563428E-4	0.0033318734255087263	6.036002582443345E-6	0.34199387031865747	1.4486406197864027E-4
80-81	7.00176299563428E-4	0.0033379094280911697	1.207200516488669E-5	0.3432071068377286	1.4486406197864027E-4
82-83	7.00176299563428E-4	0.003349981433256056	1.207200516488669E-5	0.34363566302108206	1.4486406197864027E-4
84-85	7.122483047283147E-4	0.0033560174358384996	1.207200516488669E-5	0.3438952111321272	1.4486406197864027E-4
86-87	7.303563124756447E-4	0.0034344854694102634	1.207200516488669E-5	0.34396160716053403	1.4486406197864027E-4
88-89	7.363923150580881E-4	0.0036397095572133368	1.207200516488669E-5	0.34399178717344625	1.4486406197864027E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11735	0.0	53.64709	1
GTATCAA	22530	0.0	38.895756	1
TCAACGC	28860	0.0	29.769104	4
ATCAACG	28955	0.0	29.671072	3
TATCAAC	29475	0.0	29.382765	2
CAACGCA	29450	0.0	29.187407	5
AACGCAG	30190	0.0	28.613634	6
CGCAGAG	35155	0.0	24.438671	8
ACGCAGA	35460	0.0	24.240526	7
CTTATAC	8365	0.0	22.29164	1
GCAGAGT	40435	0.0	21.120811	9
GTACATG	28015	0.0	20.792559	1
TACATGG	27770	0.0	20.590979	2
GAGTACT	23260	0.0	20.371115	12-13
TATACAC	10995	0.0	20.341516	3
ACATGGG	28025	0.0	20.111603	3
AGAGTAC	36555	0.0	19.712564	10-11
GTGGTAT	5200	0.0	19.163465	1
CAGAGTA	39380	0.0	18.673101	10-11
AGTACTT	25475	0.0	18.499166	12-13
>>END_MODULE