FastQCFastQC Report
Fri 27 May 2016
SRR2139045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12344306
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161450.051.4042431
GTATCAA305250.039.3421751
TCAACGC383000.030.6596954
ATCAACG384350.030.585113
CAACGCA390650.030.0705175
TATCAAC397100.029.8642272
AACGCAG403700.029.1834476
ACGCAGA462400.025.432347
CGCAGAG484300.024.2380128
GCAGAGT545250.021.4036169
GTACATG388900.020.116061
GAGTACT321600.019.74436412-13
AGAGTAC491300.019.6727510-11
TACATGG389150.019.6512582
ACATGGG389650.019.0744383
GTGGTAT83250.019.0107021
CAGAGTA529550.018.60408610-11
AGTACTT354950.017.84695212-13
TGGTATC80250.017.6370092
GTACTTT366800.017.27668214-15
Produced by FastQC (version 0.11.5)