##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139045_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12344306
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.675527891158886	34.0	31.0	34.0	31.0	34.0
2	32.822026041804214	34.0	31.0	34.0	31.0	34.0
3	32.86705238836432	34.0	31.0	34.0	31.0	34.0
4	36.33055426526206	37.0	37.0	37.0	35.0	37.0
5	36.266945181041365	37.0	37.0	37.0	35.0	37.0
6	36.30019054939176	37.0	37.0	37.0	35.0	37.0
7	36.25261533536191	37.0	37.0	37.0	35.0	37.0
8	36.23194418544064	37.0	37.0	37.0	35.0	37.0
9	37.913351953524156	39.0	38.0	39.0	35.0	39.0
10-11	37.8851830147438	39.0	38.0	39.0	35.0	39.0
12-13	37.86162336708115	39.0	38.0	39.0	35.0	39.0
14-15	39.28016601338301	41.0	39.0	41.0	36.0	41.0
16-17	39.266210874876236	41.0	39.0	41.0	36.0	41.0
18-19	39.29314576291288	41.0	39.0	41.0	36.0	41.0
20-21	39.20390153139846	41.0	39.0	41.0	36.0	41.0
22-23	39.20290538045728	40.0	39.0	41.0	36.0	41.0
24-25	39.11457825932574	40.0	39.0	41.0	36.0	41.0
26-27	38.971362672741705	40.0	39.0	41.0	35.0	41.0
28-29	38.84171598819685	40.0	38.5	41.0	35.0	41.0
30-31	38.7234522611395	40.0	38.0	41.0	35.0	41.0
32-33	38.6311519797073	40.0	38.0	41.0	35.0	41.0
34-35	38.486233552267365	40.0	38.0	41.0	34.5	41.0
36-37	38.62515532536362	40.0	38.0	41.0	35.0	41.0
38-39	38.68403319412418	40.0	38.0	41.0	35.0	41.0
40-41	38.66107873719871	40.0	38.0	41.0	35.0	41.0
42-43	38.62300320919021	40.0	38.0	41.0	35.0	41.0
44-45	38.54775181439313	40.0	38.0	41.0	34.0	41.0
46-47	38.4785921735729	40.0	38.0	41.0	34.0	41.0
48-49	38.386182230688284	40.0	38.0	41.0	34.0	41.0
50-51	38.28516038538034	40.0	38.0	41.0	34.0	41.0
52-53	38.167640686610156	40.0	37.0	41.0	33.5	41.0
54-55	38.02264697442341	40.0	37.0	41.0	33.0	41.0
56-57	37.86652718139328	40.0	37.0	41.0	33.0	41.0
58-59	37.669678370721556	39.0	36.0	41.0	33.0	41.0
60-61	37.43017595949956	39.0	36.0	41.0	33.0	41.0
62-63	37.22153062755862	39.0	35.0	40.0	32.0	41.0
64-65	36.97352060408542	38.0	35.0	40.0	32.0	41.0
66-67	36.685487906560326	38.0	35.0	40.0	32.0	41.0
68-69	36.32055680886116	37.0	35.0	40.0	31.0	41.0
70-71	36.044599219772465	37.0	35.0	39.0	31.0	41.0
72-73	35.7167409210267	36.0	34.5	39.0	31.0	40.0
74-75	35.39049822321624	36.0	34.0	38.5	31.0	40.0
76-77	34.37479166908684	35.0	33.5	37.0	29.5	39.0
78-79	34.66458866755923	35.0	34.0	37.0	30.0	39.0
80-81	34.507055170527394	35.0	34.0	37.0	30.0	39.0
82-83	34.24067222971722	35.0	34.0	36.0	30.0	37.5
84-85	33.9829222668678	35.0	34.0	36.0	30.0	37.0
86-87	33.76795638758129	35.0	33.5	35.0	30.0	37.0
88-89	33.59638302264803	35.0	33.0	35.0	30.0	36.0
90-91	33.41684808148581	35.0	33.0	35.0	30.0	36.0
92-93	33.28160679984631	34.5	33.0	35.0	29.0	36.0
94-95	33.19744129657644	34.0	33.0	35.0	29.0	35.5
96-97	33.18338176550655	34.0	33.0	35.0	29.0	35.0
98-99	33.19810357754585	34.0	33.0	35.0	29.5	35.0
100-101	32.20628781571469	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	1.0
14	0.0
15	1.0
16	1.0
17	1.0
18	3.0
19	8.0
20	45.0
21	321.0
22	1427.0
23	4904.0
24	12834.0
25	27002.0
26	47950.0
27	76143.0
28	109158.0
29	146877.0
30	188745.0
31	236087.0
32	299434.0
33	389334.0
34	547518.0
35	812287.0
36	1417987.0
37	2856521.0
38	3576631.0
39	1524805.0
40	68276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.114582725765665	21.30999852239543	15.267203400045885	24.308215351793024
2	13.784362716027779	21.988046976016175	41.33008785318699	22.897502454769057
3	16.609636314854203	27.260281341094643	31.49244380242094	24.637638541630213
4	11.857818136836078	19.01385602735124	39.34268301742107	29.78564281839161
5	12.878255428576612	38.05011606972738	35.84601448570936	13.225614015986645
6	29.011680204894503	38.72599054592305	19.06939427285653	13.192934976325924
7	25.63107648677479	33.12963614635544	23.86757487501928	17.37171249185049
8	24.258642000611456	36.68077411561249	21.401559553044134	17.659024330731917
9	25.572875461771606	16.745882676595993	21.48557399662646	36.19566786500594
10-11	23.811343442022583	26.941732993033124	29.29495989553311	19.95196366941118
12-13	24.922486529416883	24.705917043858115	29.240696074773258	21.13090035195174
14-15	22.06921742942821	25.827451152577446	27.08399238087708	25.019339037117263
16-17	21.42278213209125	28.93639843589816	27.625390982929726	22.015428449080858
18-19	21.47131409990275	28.04197158122713	29.49837192130298	20.988342397567138
20-21	22.44505043153424	27.155277310249044	29.336089574544893	21.06358268367182
22-23	22.135276191470023	27.133616111381087	29.15109548271872	21.580012214430173
24-25	21.925304907325824	27.309747496385956	29.21148444890183	21.55346314738639
26-27	21.846047715913908	27.43537765673822	29.152052996819442	21.566521630528428
28-29	21.773595573244116	27.463519175897854	29.18530610664158	21.57757914421645
30-31	22.064173634530118	27.27623987304354	29.131678723737892	21.527907768688447
32-33	21.88469182424379	27.42362912378125	29.012271646792293	21.679407405182662
34-35	22.02348611218623	27.455154305091074	28.977492080740685	21.54386750198201
36-37	22.018979921333433	27.450943428779357	28.879500748131125	21.650575901756085
38-39	21.85157824173027	27.55096588195311	28.948768443397043	21.648687432919573
40-41	21.994303465583805	27.4095838252309	28.848497151732793	21.747615557452505
42-43	21.93738078317772	27.577765368149727	28.589592361243852	21.895261487428698
44-45	22.02044351102559	27.640006587188992	28.37900369692098	21.96054620486444
46-47	22.13413937591685	27.608292430291716	28.117409072856038	22.140159120935405
48-49	22.16741458277772	27.803206163297546	27.955925116346474	22.07345413757826
50-51	22.138252001608688	27.885197933881294	27.856928334230695	22.119621730279317
52-53	22.314086182112433	27.77150291316413	27.748853315116122	22.16555758960731
54-55	22.278203526133787	27.706049676924305	27.732588828223033	22.28315796871888
56-57	22.24561074890506	27.89587638794521	27.655723321897263	22.202789541252464
58-59	22.154241304996095	27.897810612962104	27.805392628579106	22.142555453462702
60-61	22.331491286010063	27.858657031204043	27.673138154447297	22.136713528338596
62-63	22.271775986320772	27.981445317717107	27.67188067762675	22.074898018335375
64-65	22.276126309706783	27.875944369143195	27.618849677955886	22.229079643194137
66-67	22.306959043729627	27.94296654320258	27.47263170790366	22.277442705164134
68-69	22.281738986679727	27.885457308291144	27.540816099993005	22.29198760503612
70-71	22.265874458487428	27.724202727001888	27.578331243008552	22.43159157150213
72-73	22.310222046915573	27.712848656329115	27.523911439447176	22.45301785730814
74-75	22.176118462039778	27.857504809606397	27.494389771048787	22.471986957305035
76-77	22.375366828673336	27.74172744437774	27.43492462118189	22.447981105767038
78-79	22.307508471286532	27.764429795161895	27.420262614161437	22.507799119390135
80-81	22.298226592528454	27.726204089995893	27.45584479233376	22.519724525141896
82-83	22.35848491905491	27.6765622755168	27.57703394369676	22.387918861731528
84-85	22.451989609643523	27.65803054044671	27.50896690393826	22.381012945971513
86-87	22.287655464205503	27.710559031632588	27.560904109729208	22.440881394432704
88-89	22.328907164415767	27.666055151375286	27.54188206328005	22.463155620928898
90-91	22.413391522484417	27.66874912459198	27.520559320010623	22.397300032912977
92-93	22.475103424378492	27.55762565746746	27.599751987298017	22.367518930856036
94-95	22.275289609242833	27.586925848297806	27.683122264539712	22.45466227791965
96-97	22.232002521874556	27.67383571709735	27.6704349405499	22.423726820478194
98-99	22.483141834955596	28.232742230711448	27.77521696635048	21.508898967982475
100-101	23.056793015641862	30.881585911859556	24.935870633762732	21.12575043873585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4355.0
1	3561.0
2	3968.0
3	5427.0
4	7310.0
5	11190.5
6	14304.5
7	17341.0
8	19708.5
9	19708.5
10	17757.5
11	15901.0
12	17740.5
13	24042.0
14	38951.5
15	59590.0
16	75754.0
17	83895.0
18	84284.5
19	79056.5
20	71681.0
21	64661.5
22	60727.5
23	63567.0
24	73333.0
25	89732.5
26	110809.0
27	134475.0
28	162334.0
29	191498.0
30	218397.5
31	245859.5
32	273911.0
33	300889.5
34	325678.5
35	349345.0
36	375425.0
37	398278.5
38	416338.5
39	430809.5
40	443624.5
41	456453.0
42	463775.0
43	470348.0
44	479739.0
45	489934.5
46	498200.0
47	502543.0
48	502448.0
49	495198.5
50	483493.0
51	470796.0
52	449592.0
53	421885.0
54	397076.5
55	372261.0
56	344681.5
57	311116.0
58	275437.5
59	238443.0
60	199522.0
61	164106.0
62	131475.5
63	102765.0
64	78403.5
65	58349.5
66	44590.5
67	34248.5
68	24813.0
69	17598.0
70	12760.0
71	9230.0
72	6486.0
73	4543.0
74	3296.5
75	2294.5
76	1452.0
77	853.5
78	552.5
79	365.5
80	236.0
81	152.0
82	113.0
83	77.0
84	52.5
85	37.5
86	26.0
87	18.5
88	11.5
89	6.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.6201801867192857E-5
2	0.007987488320526079
3	1.6201801867192857E-5
4	2.4302702800789284E-5
5	1.6201801867192857E-5
6	1.6201801867192857E-5
7	1.6201801867192857E-5
8	0.0
9	0.0
10-11	8.708468503616162E-4
12-13	0.0
14-15	8.343927961604322E-4
16-17	7.857873905588535E-4
18-19	8.100900933596429E-6
20-21	5.103724284824431E-4
22-23	0.001093745050297284
24-25	0.004712072325245627
26-27	0.016714371254718346
28-29	0.3256922054169715
30-31	0.3127735647337323
32-33	0.3212634760756109
34-35	0.1855908646248163
36-37	0.04668849040233657
38-39	0.036460574023613995
40-41	0.03298445330557795
42-43	0.029129519194822213
44-45	0.016128774423482553
46-47	0.008555207659824895
48-49	0.006504003134612242
50-51	0.0025805069542068943
52-53	0.001928792465326799
54-55	0.0015827658978750337
56-57	5.919743998461927E-4
58-59	0.0030727857439129674
60-61	0.0028197930433550363
62-63	5.251741789965642E-4
64-65	2.1916100964920266E-4
66-67	0.007728774846317437
68-69	0.006976206713893896
70-71	0.009306179218399388
72-73	0.009900364647610854
74-75	0.007811510457144449
76-77	0.004656157979471377
78-79	3.6676864791178066E-4
80-81	5.142234197914311E-4
82-83	7.650966317045842E-4
84-85	0.0
86-87	0.001872442082961872
88-89	0.0013882577122248472
90-91	0.001181639155131155
92-93	0.012288224053614828
94-95	1.4629376134047567E-4
96-97	7.72903163404316E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	230.0
20-21	821.0
22-23	1716.0
24-25	3679.0
26-27	5365.0
28-29	9263.0
30-31	23796.0
32-33	32118.0
34-35	46216.0
36-37	62283.0
38-39	71976.0
40-41	78802.0
42-43	94808.0
44-45	113618.0
46-47	112451.0
48-49	97837.0
50-51	85908.0
52-53	78506.0
54-55	84508.0
56-57	89958.0
58-59	91590.0
60-61	92538.0
62-63	91597.0
64-65	99269.0
66-67	105300.0
68-69	105747.0
70-71	104228.0
72-73	101420.0
74-75	102868.0
76-77	104061.0
78-79	110845.0
80-81	114344.0
82-83	111797.0
84-85	112397.0
86-87	118680.0
88-89	128645.0
90-91	140685.0
92-93	146220.0
94-95	162886.0
96-97	241444.0
98-99	1054451.0
100-101	7809435.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.05560440104762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.53870164995918	73.5363537842139
2	7.758289320860902	12.88738817324591
3	2.006054803449551	4.998422824863816
4	0.7818845627315777	2.5975957971808006
5	0.3580991230995192	1.4871069552257858
6	0.185100187973051	0.9224164792109566
7	0.11236334796854765	0.6532684044637065
8	0.0658613287774083	0.4376121974611004
9	0.04348494698460845	0.32505016987387514
>10	0.14607393861831322	1.85634511477851
>50	0.003277169530215812	0.1810085908224834
>100	7.40039176382953E-4	0.08287344353461687
>500	6.958087075836259E-5	0.034558065124477066
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.100900933596429E-6	0.0
2	0.0	7.290810840236787E-5	0.0	1.6201801867192857E-5	0.0
3	0.0	7.290810840236787E-5	0.0	2.4302702800789288E-5	0.0
4	0.0	1.3771531587113928E-4	0.0	4.0504504667982145E-5	8.100900933596429E-6
5	0.0	1.6201801867192858E-4	0.0	4.0504504667982145E-5	8.100900933596429E-6
6	0.0	1.6201801867192858E-4	0.0	4.0504504667982145E-5	8.100900933596429E-6
7	0.0	1.7821982053912143E-4	0.0	4.0504504667982145E-5	8.100900933596429E-6
8	0.0	1.8632072147271788E-4	0.0	4.0504504667982145E-5	2.4302702800789288E-5
9	0.0	3.078342354766643E-4	0.0	4.8605405601578575E-5	2.4302702800789288E-5
10-11	0.0	3.078342354766643E-4	0.0	7.290810840236787E-5	2.4302702800789288E-5
12-13	0.0	3.2403603734385716E-4	0.0	1.1746306353714822E-4	2.4302702800789288E-5
14-15	0.0	4.414991008810054E-4	0.0	1.5796756820513037E-4	2.8353153267587503E-5
16-17	0.0	4.698522541485929E-4	0.0	1.903711719395161E-4	3.2403603734385715E-5
18-19	4.050450466798214E-6	4.941549569493822E-4	0.0	2.1872432520710357E-4	4.0504504667982145E-5
20-21	8.100900933596429E-6	5.346594616173643E-4	0.0	2.592288298750857E-4	4.0504504667982145E-5
22-23	8.100900933596429E-6	5.751639662853465E-4	0.0	5.10356758816575E-4	4.0504504667982145E-5
24-25	8.100900933596429E-6	6.440216242209161E-4	0.0	0.0014824648708481464	4.455495513478036E-5
26-27	8.100900933596429E-6	6.966774802892929E-4	0.0	0.003831726141591111	5.6706306535175005E-5
28-29	8.100900933596429E-6	7.047783812228893E-4	0.0	0.018587517192137006	5.6706306535175005E-5
30-31	8.100900933596429E-6	7.655351382248626E-4	0.0	0.025837823527705812	5.6706306535175005E-5
32-33	8.100900933596429E-6	7.695855886916608E-4	0.0	0.02815468119471439	8.100900933596429E-5
34-35	8.100900933596429E-6	8.708468503616162E-4	0.0	0.03177983436249879	8.100900933596429E-5
36-37	8.100900933596429E-6	9.842594634319661E-4	0.0	0.03693200735626612	8.100900933596429E-5
38-39	8.100900933596429E-6	0.001040965769967141	0.0	0.04616703442056605	8.100900933596429E-5
40-41	8.100900933596429E-6	0.0011422270316370966	0.0	0.06814072820294637	8.100900933596429E-5
42-43	8.100900933596429E-6	0.0012029837886390698	0.0	0.07677223814769335	8.100900933596429E-5
44-45	8.100900933596429E-6	0.0013731027082445947	0.0	0.08889928684528721	8.100900933596429E-5
46-47	8.100900933596429E-6	0.0014986666727153394	0.0	0.1032378814977529	9.316036073635893E-5
48-49	8.100900933596429E-6	0.001510818024115734	0.0	0.1161709698382396	1.2961441493754286E-4
50-51	8.100900933596429E-6	0.0016971387455884518	0.0	0.1279375284442884	1.2961441493754286E-4
52-53	8.100900933596429E-6	0.0017092900969888465	0.0	0.146934141133572	1.4986666727153394E-4
54-55	8.100900933596429E-6	0.0017335927997896357	0.0	0.18865378094159363	1.70118919605525E-4
56-57	8.100900933596429E-6	0.001757895502590425	0.0	0.23234599012694598	1.70118919605525E-4
58-59	8.100900933596429E-6	0.0017781477549244163	0.0	0.26321447313441515	1.7821982053912143E-4
60-61	8.100900933596429E-6	0.0018267531605259948	0.0	0.28178173807421814	1.7821982053912143E-4
62-63	8.100900933596429E-6	0.0018753585661275734	0.0	0.29304604082238406	1.7821982053912143E-4
64-65	8.100900933596429E-6	0.002061679287600291	0.0	0.3007661994121014	1.7821982053912143E-4
66-67	8.100900933596429E-6	0.0021062342427350714	0.0	0.30568344627879446	1.8632072147271788E-4
68-69	8.100900933596429E-6	0.002158890098803448	0.0	0.3106979039566906	1.8632072147271788E-4
70-71	8.100900933596429E-6	0.0021993946034714303	0.0	0.31548958685891293	1.8632072147271788E-4
72-73	8.100900933596429E-6	0.002235848657672614	0.0	0.31915524453136535	1.944216224063143E-4
74-75	8.100900933596429E-6	0.002300655865141386	0.0	0.32250091661694064	1.944216224063143E-4
76-77	8.100900933596429E-6	0.0023695135230769556	0.0	0.32546179590817015	1.944216224063143E-4
78-79	8.100900933596429E-6	0.0024221693791453324	0.0	0.3280459833059874	1.944216224063143E-4
80-81	8.100900933596429E-6	0.002434320730545727	0.0	0.32929352204976126	2.0657297380670894E-4
82-83	8.100900933596429E-6	0.0024383711810125252	0.0	0.32979172745717744	2.3492612707429645E-4
84-85	8.100900933596429E-6	0.0024464720819461216	0.0	0.3300428553861189	2.3492612707429645E-4
86-87	8.100900933596429E-6	0.0025234306408152877	0.0	0.33013196529638844	2.3492612707429645E-4
88-89	1.6201801867192857E-5	0.0026894991099540144	0.0	0.33016841935058966	2.3492612707429645E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16145	0.0	51.404243	1
GTATCAA	30525	0.0	39.342175	1
TCAACGC	38300	0.0	30.659695	4
ATCAACG	38435	0.0	30.58511	3
CAACGCA	39065	0.0	30.070517	5
TATCAAC	39710	0.0	29.864227	2
AACGCAG	40370	0.0	29.183447	6
ACGCAGA	46240	0.0	25.43234	7
CGCAGAG	48430	0.0	24.238012	8
GCAGAGT	54525	0.0	21.403616	9
GTACATG	38890	0.0	20.11606	1
GAGTACT	32160	0.0	19.744364	12-13
AGAGTAC	49130	0.0	19.67275	10-11
TACATGG	38915	0.0	19.651258	2
ACATGGG	38965	0.0	19.074438	3
GTGGTAT	8325	0.0	19.010702	1
CAGAGTA	52955	0.0	18.604086	10-11
AGTACTT	35495	0.0	17.846952	12-13
TGGTATC	8025	0.0	17.637009	2
GTACTTT	36680	0.0	17.276682	14-15
>>END_MODULE