FastQCFastQC Report
Fri 27 May 2016
SRR2139044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10081346
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133650.051.5025671
GTATCAA256600.038.0077131
ATCAACG319150.029.8647613
TCAACGC319500.029.8055154
CAACGCA326100.029.189285
TATCAAC329850.029.1818262
AACGCAG335300.028.4894946
ACGCAGA381400.024.9681767
CGCAGAG385250.024.7406568
GCAGAGT446500.021.2333039
GTACATG300450.020.8503821
TACATGG298250.020.606272
CTTATAC105450.020.5393221
ACATGGG303750.019.7565633
GAGTACT273600.019.60313612-13
AGAGTAC406850.019.44860310-11
CAGAGTA438700.018.38923510-11
TATACAC140200.018.3486923
CATGGGG229000.017.8959774
TATGCCG63700.017.76620742-43
Produced by FastQC (version 0.11.5)