##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139044_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10081346
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68274504217988	34.0	31.0	34.0	31.0	34.0
2	32.83046519780196	34.0	31.0	34.0	31.0	34.0
3	32.87565648475908	34.0	31.0	34.0	31.0	34.0
4	36.33391067026169	37.0	37.0	37.0	35.0	37.0
5	36.272645835189074	37.0	37.0	37.0	35.0	37.0
6	36.30669862932985	37.0	37.0	37.0	35.0	37.0
7	36.26203713274001	37.0	37.0	37.0	35.0	37.0
8	36.243790759686256	37.0	37.0	37.0	35.0	37.0
9	37.923767619919005	39.0	38.0	39.0	35.0	39.0
10-11	37.899942477919126	39.0	38.0	39.0	35.0	39.0
12-13	37.87099252421254	39.0	38.0	39.0	35.0	39.0
14-15	39.28910921220242	41.0	39.0	41.0	36.0	41.0
16-17	39.27582889229275	41.0	39.0	41.0	36.0	41.0
18-19	39.30900903510305	41.0	39.0	41.0	36.0	41.0
20-21	39.23213157364029	41.0	39.0	41.0	36.0	41.0
22-23	39.22736716419523	40.5	39.0	41.0	36.0	41.0
24-25	39.13371574013897	40.5	39.0	41.0	36.0	41.0
26-27	38.99121011304628	40.0	39.0	41.0	35.0	41.0
28-29	38.86084767599639	40.0	39.0	41.0	35.0	41.0
30-31	38.74189333830505	40.0	38.0	41.0	35.0	41.0
32-33	38.64548538112969	40.0	38.0	41.0	35.0	41.0
34-35	38.50234716526553	40.0	38.0	41.0	34.5	41.0
36-37	38.63863958522387	40.0	38.0	41.0	35.0	41.0
38-39	38.69460049284288	40.0	38.0	41.0	35.0	41.0
40-41	38.68005891902838	40.0	38.0	41.0	35.0	41.0
42-43	38.64918118928789	40.0	38.0	41.0	35.0	41.0
44-45	38.5799493388227	40.0	38.0	41.0	34.5	41.0
46-47	38.514485209336215	40.0	38.0	41.0	34.0	41.0
48-49	38.43104384393932	40.0	38.0	41.0	34.0	41.0
50-51	38.334111139731874	40.0	38.0	41.0	34.0	41.0
52-53	38.22755177499651	40.0	37.5	41.0	34.0	41.0
54-55	38.093211178581	40.0	37.0	41.0	33.5	41.0
56-57	37.94227832979668	40.0	37.0	41.0	33.0	41.0
58-59	37.75811288834646	39.5	36.5	41.0	33.0	41.0
60-61	37.53221467842359	39.0	36.0	41.0	33.0	41.0
62-63	37.3384937732771	39.0	35.5	40.5	33.0	41.0
64-65	37.10754470914932	38.5	35.0	40.0	32.0	41.0
66-67	36.832667491703184	38.0	35.0	40.0	32.0	41.0
68-69	36.4825693500743	37.5	35.0	40.0	31.5	41.0
70-71	36.208638649464405	37.0	35.0	39.5	31.0	41.0
72-73	35.88350939165319	36.5	35.0	39.0	31.0	40.5
74-75	35.55669189412524	36.0	34.0	39.0	31.0	40.0
76-77	34.52831196730579	35.0	33.5	37.0	29.5	39.0
78-79	34.81422001617642	35.0	34.0	37.0	30.5	39.0
80-81	34.648217976402414	35.0	34.0	37.0	31.0	39.0
82-83	34.37152550570329	35.0	34.0	36.0	30.0	38.5
84-85	34.105449938451166	35.0	34.0	36.0	30.0	37.0
86-87	33.879151394355496	35.0	34.0	35.5	30.0	37.0
88-89	33.69454329006637	35.0	33.5	35.0	30.0	36.5
90-91	33.50665451630333	35.0	33.0	35.0	30.0	36.0
92-93	33.36427297565142	35.0	33.0	35.0	30.0	36.0
94-95	33.27155715832799	34.0	33.0	35.0	29.5	35.5
96-97	33.2513839009836	34.0	33.0	35.0	30.0	35.0
98-99	33.25918808341291	34.0	33.0	35.0	30.0	35.0
100-101	32.25725677528155	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	4.0
19	12.0
20	45.0
21	253.0
22	1243.0
23	4088.0
24	10468.0
25	21716.0
26	38422.0
27	59607.0
28	85567.0
29	115392.0
30	147524.0
31	186635.0
32	235882.0
33	310258.0
34	435964.0
35	632891.0
36	1089049.0
37	2243707.0
38	3002969.0
39	1395804.0
40	63843.0
41	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.8381510602008	21.502934380283584	15.296788275770743	24.362126283744878
2	13.481678002542127	22.093345659401145	41.795359431887185	22.629616906169545
3	16.330231729992377	27.716212469665507	31.79869352750055	24.15486227284157
4	11.657993877797992	19.310795985454316	39.7240247824198	29.307185354327896
5	12.554981502964138	38.35245197937379	36.10980479291206	12.982761724750022
6	28.404007600176367	39.22748403114863	19.308584320842108	13.059924047832903
7	25.03257253868407	33.518394618971975	24.124122326931573	17.324910515412377
8	23.876454592472076	36.90546877371335	21.787467665527995	17.430608968286577
9	25.44984568528845	16.817734457283777	21.666422320987692	36.06599753644007
10-11	23.451851558679103	27.145188409424144	29.68974712676515	19.71321290513161
12-13	24.57744233756088	24.91474848695799	29.587720726974354	20.920088448506778
14-15	21.763532099273707	26.13121677660063	27.374045129886536	24.731205994239126
16-17	21.06364914882714	29.242145778119532	27.96202877694131	21.732176296112016
18-19	21.095640403573093	28.33454977142933	29.89530366282439	20.674506162173188
20-21	21.978990453168358	27.474036896282545	29.69125993943947	20.855712711109632
22-23	21.649246697722397	27.494038844349046	29.594400345514142	21.26231411241442
24-25	21.498678670838277	27.75084973068066	29.553643741118226	21.196827857362837
26-27	21.445797810635668	27.92345292114072	29.462361491700364	21.168387776523254
28-29	21.28921555805498	27.955966922878588	29.5375455719679	21.217271947098528
30-31	21.71020713270957	27.69525575851757	29.502042657518206	21.092494451254662
32-33	21.46596502600551	27.869560280195575	29.398564360443263	21.26591033335565
34-35	21.531969375962877	27.82297087347803	29.470503069602966	21.174556680956126
36-37	21.42304977362148	27.99063275164554	29.3725908724362	21.21372660229678
38-39	21.36827772928847	27.882003249427136	29.430312651806506	21.319406369477896
40-41	21.53094540483712	27.741129272446337	29.364591420869214	21.36333390184733
42-43	21.587915438354884	27.84035434269505	29.062936979712163	21.5087932392379
44-45	21.60002021155765	28.015595204745825	28.859696947780407	21.524687635916123
46-47	21.72858194903977	27.945486495932663	28.496468832796683	21.82946272223088
48-49	21.716563930604533	28.12832617660445	28.374597726422408	21.78051216636861
50-51	21.6721451910591	28.18569164883392	28.35056751900546	21.79159564110152
52-53	21.75351529300384	28.162736280405802	28.266140224419317	21.817608202171037
54-55	21.7201539656868	28.124548083532574	28.22750166993895	21.92779628084168
56-57	21.86335496812486	28.190813599469184	28.06845231046567	21.87737912194029
58-59	21.629084888655882	28.397205829936485	28.27741246328023	21.69629681812741
60-61	21.919903860999774	28.421108664159515	28.012317252012046	21.646670222828664
62-63	21.767539715611303	28.56932849679391	27.958221250291814	21.704910537302972
64-65	21.725553446663916	28.460150617881247	27.972895262498266	21.841400672956578
66-67	21.848165536660378	28.42687589965045	27.850909885560526	21.874048678128645
68-69	21.786488332888528	28.308635952123634	27.948790316741505	21.956085398246326
70-71	21.792609730490355	28.09682867802022	28.025200785977916	22.085360805511513
72-73	21.836258879803427	28.13904238825503	27.95987998615682	22.06481874578472
74-75	21.72622521826527	28.22178547506427	27.95036086112099	22.10162844554947
76-77	21.8793271306521	28.129814587728546	27.893259716887357	22.09759856473199
78-79	21.833360296056227	28.110104004428237	27.891469397944412	22.165066301571123
80-81	21.840834767526925	28.172129102859746	27.861077062855273	22.125959066758057
82-83	21.87695799798199	28.135795453651074	27.98856565383084	21.9986808945361
84-85	22.01693661501629	28.0969015829753	27.889851767170782	21.99631003483763
86-87	21.87168922515803	28.095224413595982	27.94776428977958	22.08532207146641
88-89	21.857315346561613	28.055760196622977	27.97214097666411	22.114783480151303
90-91	21.985207209844717	28.04111360630116	27.951723784321004	22.02195539953312
92-93	22.062248640832628	27.945933321695264	27.977797426484308	22.0140206109878
94-95	21.880688191951823	27.98692707638396	28.057669995777523	22.074714735886694
96-97	21.813692778526903	28.021948220178423	28.102845224413777	22.061513776880897
98-99	22.036945179072283	28.620442310050837	28.115129152365835	21.227483358511044
100-101	22.53055816039467	31.282097280547966	25.38150609532352	20.805838463733846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2949.0
1	2690.0
2	3699.0
3	5263.5
4	7038.5
5	10556.0
6	13399.5
7	16008.0
8	18229.0
9	18587.0
10	17039.5
11	15387.0
12	16757.5
13	21952.5
14	34591.5
15	51922.0
16	65471.5
17	72378.5
18	72668.0
19	68404.5
20	62385.5
21	56833.0
22	54631.0
23	58192.5
24	67215.5
25	82683.5
26	102899.0
27	123925.5
28	147716.0
29	173681.5
30	199085.5
31	224793.0
32	249061.5
33	271359.5
34	292713.0
35	312055.0
36	333350.5
37	353526.0
38	364990.0
39	372564.0
40	382586.0
41	391604.5
42	394822.5
43	398273.0
44	404711.5
45	412156.5
46	417170.5
47	415863.5
48	406648.5
49	394343.5
50	383375.5
51	369760.0
52	350969.0
53	329229.5
54	308076.0
55	283722.5
56	258466.0
57	233089.5
58	204574.0
59	174183.5
60	144311.0
61	117374.5
62	92557.5
63	70371.0
64	52600.5
65	40115.5
66	31051.5
67	23807.5
68	18217.0
69	13142.0
70	9091.5
71	6675.0
72	4811.5
73	3404.0
74	2408.0
75	1632.0
76	1073.5
77	695.0
78	422.5
79	259.5
80	175.0
81	115.5
82	78.0
83	53.5
84	35.0
85	24.5
86	19.5
87	15.5
88	8.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.919310377800742E-6
2	0.008064399337152002
3	9.919310377800742E-6
4	5.951586226680445E-5
5	9.919310377800742E-6
6	9.919310377800742E-6
7	9.919310377800742E-6
8	0.0
9	0.0
10-11	6.893920712571516E-4
12-13	0.0
14-15	6.596341401237493E-4
16-17	7.935448302240593E-4
18-19	0.0
20-21	5.9021641400775E-4
22-23	0.0012598984492088556
24-25	0.004698522084095706
26-27	0.0179632164551203
28-29	0.32377073420660457
30-31	0.3112611860731801
32-33	0.3202765010263272
34-35	0.18509893329207466
36-37	0.04563934518465976
38-39	0.03489304520901253
40-41	0.03258813900655697
42-43	0.02873407010436165
44-45	0.015508308358797034
46-47	0.00830923106909403
48-49	0.0062326612808155935
50-51	0.0026352984355449477
52-53	0.001995635314267647
54-55	0.0016320916279013275
56-57	6.125957898807379E-4
58-59	0.00283216225540373
60-61	0.0026363954220804974
62-63	4.671277423845655E-4
64-65	2.0466661485569215E-4
66-67	0.008071301092011396
68-69	0.007199302214320436
70-71	0.009620029418708547
72-73	0.010199446733508309
74-75	0.007851700304624346
76-77	0.004877369981190845
78-79	4.730998497508607E-4
80-81	5.783799341716567E-4
82-83	6.611224726232332E-4
84-85	0.0
86-87	0.00215642625677523
88-89	0.0016427739055509722
90-91	0.0011157829355877107
92-93	0.012799337160314901
94-95	1.1668072440610026E-4
96-97	5.598889796142323E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	185.0
20-21	691.0
22-23	1503.0
24-25	4444.0
26-27	4958.0
28-29	8502.0
30-31	22803.0
32-33	29620.0
34-35	42223.0
36-37	57631.0
38-39	67095.0
40-41	72157.0
42-43	85355.0
44-45	102289.0
46-47	100985.0
48-49	87978.0
50-51	79692.0
52-53	72787.0
54-55	78122.0
56-57	83976.0
58-59	86337.0
60-61	86770.0
62-63	87429.0
64-65	94318.0
66-67	101021.0
68-69	96068.0
70-71	92046.0
72-73	88768.0
74-75	90988.0
76-77	92569.0
78-79	97426.0
80-81	99204.0
82-83	96911.0
84-85	98902.0
86-87	103359.0
88-89	109109.0
90-91	117123.0
92-93	121769.0
94-95	134464.0
96-97	194762.0
98-99	827687.0
100-101	6161320.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.01081836900325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.81100140959659	73.72273907181314
2	7.488998880605995	12.433358516873056
3	1.973380586053795	4.914358122054862
4	0.7542618320037756	2.504475677565482
5	0.3667393479505689	1.5221666700745677
6	0.19576651044944893	0.9750442944991669
7	0.10865458778320818	0.6313654376001571
8	0.07701201616452846	0.5114264388851532
9	0.051684088363110496	0.3861304624509912
>10	0.1696075617032079	2.1822178012767908
>50	0.0022398352070791883	0.12135892699550123
>100	6.109981630697503E-4	0.07382815054137865
>500	4.23459555405325E-5	0.021530429369705464
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9838620755601485E-5
2	0.0	1.9838620755601485E-5	0.0	0.0	1.9838620755601485E-5
3	0.0	1.9838620755601485E-5	0.0	0.0	1.9838620755601485E-5
4	0.0	2.9757931133402227E-5	0.0	0.0	1.9838620755601485E-5
5	0.0	2.9757931133402227E-5	0.0	0.0	1.9838620755601485E-5
6	9.919310377800742E-5	2.9757931133402227E-5	0.0	0.0	2.9757931133402227E-5
7	9.919310377800742E-5	2.9757931133402227E-5	0.0	9.919310377800742E-6	2.9757931133402227E-5
8	9.919310377800742E-5	3.967724151120297E-5	0.0	1.9838620755601485E-5	3.967724151120297E-5
9	9.919310377800742E-5	6.94351726446052E-5	0.0	4.959655188900371E-5	3.967724151120297E-5
10-11	9.919310377800742E-5	6.94351726446052E-5	0.0	4.959655188900371E-5	3.967724151120297E-5
12-13	9.919310377800742E-5	6.94351726446052E-5	0.0	4.959655188900371E-5	3.967724151120297E-5
14-15	1.0911241415580817E-4	1.4383000047811078E-4	0.0	5.9515862266804455E-5	6.447551745570483E-5
16-17	1.388703452892104E-4	1.4878965566701114E-4	0.0	8.927379340020668E-5	8.927379340020668E-5
18-19	1.388703452892104E-4	1.7358793161151298E-4	0.0	1.1407206934470854E-4	9.919310377800742E-5
20-21	1.388703452892104E-4	2.083055179338156E-4	0.0	1.6862827642261262E-4	9.919310377800742E-5
22-23	1.388703452892104E-4	2.2814413868941708E-4	0.0	4.265303462454319E-4	1.4878965566701114E-4
24-25	1.388703452892104E-4	2.6782138020062E-4	0.0	0.0012895103491140964	1.9838620755601485E-4
26-27	1.388703452892104E-4	2.975793113340223E-4	0.0	0.0038139748402643853	1.9838620755601485E-4
28-29	1.388703452892104E-4	2.975793113340223E-4	0.0	0.020111401790991003	1.9838620755601485E-4
30-31	1.4383000047811078E-4	3.07498621711823E-4	0.0	0.028547775267310536	1.9838620755601485E-4
32-33	1.4878965566701114E-4	3.124582769007234E-4	0.0	0.03141941562168385	2.083055179338156E-4
34-35	1.5870896604481188E-4	3.7197413916752783E-4	0.0	0.03553096977328225	2.1822482831161633E-4
36-37	1.5870896604481188E-4	4.265303462454319E-4	0.0	0.0413883225513736	2.2318448350051672E-4
38-39	1.5870896604481188E-4	4.463689670010334E-4	0.0	0.051649849137208464	2.3310379387831743E-4
40-41	1.5870896604481188E-4	5.058848292678378E-4	0.0	0.07769299853412431	2.3806344906721782E-4
42-43	1.6862827642261262E-4	5.207637948345389E-4	0.0	0.08797436374071478	2.479827594450186E-4
44-45	1.6862827642261262E-4	5.802796571013433E-4	0.0	0.10188123689039141	2.628617250117197E-4
46-47	1.6862827642261262E-4	6.249165538014467E-4	0.0	0.11799515659912874	2.827003457673211E-4
48-49	1.6862827642261262E-4	6.249165538014467E-4	0.0	0.1322789635431618	3.07498621711823E-4
50-51	1.7854758680041336E-4	6.993113816349524E-4	0.0	0.14544684806969227	3.07498621711823E-4
52-53	1.884668971782141E-4	7.042710368238527E-4	0.0	0.1666394546918636	3.223775872785241E-4
54-55	1.884668971782141E-4	7.141903472016535E-4	0.0	0.21504072968034227	3.3725655284522524E-4
56-57	1.884668971782141E-4	7.340289679572549E-4	0.0	0.26380405949761077	3.4221620803412563E-4
58-59	1.884668971782141E-4	7.340289679572549E-4	0.0	0.29877954788973615	3.4717586322302596E-4
60-61	1.9342655236711446E-4	7.389886231461552E-4	0.0	0.3186925634731711	3.4717586322302596E-4
62-63	2.4798275944501854E-4	7.637868990906572E-4	0.0	0.3309577907553217	3.6701448397862745E-4
64-65	2.4798275944501854E-4	8.332220717352624E-4	0.0	0.3399694842335538	3.6701448397862745E-4
66-67	2.727810353895204E-4	8.530606924908638E-4	0.0	0.34606986011590124	3.7197413916752783E-4
68-69	2.777406905784208E-4	8.530606924908638E-4	0.0	0.3515254808236916	3.769337943564282E-4
70-71	2.926196561451219E-4	8.629800028686646E-4	0.0	0.3567033608409036	3.8685310473422893E-4
72-73	3.3725655284522524E-4	8.877782788131664E-4	0.0	0.3609339467170356	4.7612689813443564E-4
74-75	3.3725655284522524E-4	9.07616899568768E-4	0.0	0.36421723845208764	4.7612689813443564E-4
76-77	3.3725655284522524E-4	9.224958651354691E-4	0.0	0.36755508639421763	4.7612689813443564E-4
78-79	3.3725655284522524E-4	9.324151755132697E-4	0.0	0.37075406399105837	4.7612689813443564E-4
80-81	3.3725655284522524E-4	9.324151755132697E-4	0.0	0.37216756571989495	4.8108655332333597E-4
82-83	3.4221620803412563E-4	9.324151755132697E-4	0.0	0.3726486522732183	5.455620707790408E-4
84-85	3.5709517360082673E-4	9.324151755132697E-4	0.0	0.3729015946878522	5.505217259679411E-4
86-87	3.6205482878972706E-4	9.373748307021702E-4	0.0	0.3729561508949301	5.554813811568416E-4
88-89	3.769337943564282E-4	9.720924170244727E-4	0.0	0.37298590882606353	5.654006915346423E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13365	0.0	51.502567	1
GTATCAA	25660	0.0	38.007713	1
ATCAACG	31915	0.0	29.864761	3
TCAACGC	31950	0.0	29.805515	4
CAACGCA	32610	0.0	29.18928	5
TATCAAC	32985	0.0	29.181826	2
AACGCAG	33530	0.0	28.489494	6
ACGCAGA	38140	0.0	24.968176	7
CGCAGAG	38525	0.0	24.740656	8
GCAGAGT	44650	0.0	21.233303	9
GTACATG	30045	0.0	20.850382	1
TACATGG	29825	0.0	20.60627	2
CTTATAC	10545	0.0	20.539322	1
ACATGGG	30375	0.0	19.756563	3
GAGTACT	27360	0.0	19.603136	12-13
AGAGTAC	40685	0.0	19.448603	10-11
CAGAGTA	43870	0.0	18.389235	10-11
TATACAC	14020	0.0	18.348692	3
CATGGGG	22900	0.0	17.895977	4
TATGCCG	6370	0.0	17.766207	42-43
>>END_MODULE