FastQCFastQC Report
Fri 27 May 2016
SRR2139043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12548199
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162750.050.234791
GTATCAA308000.038.291231
TCAACGC389400.029.7046364
ATCAACG389600.029.6893863
CAACGCA397950.029.1415635
TATCAAC408100.028.59552
AACGCAG409150.028.3647296
ACGCAGA470000.024.7105757
CGCAGAG475750.024.4298748
GCAGAGT549750.021.0329289
GTACATG369200.019.9924351
AGAGTAC506900.019.75353810-11
TACATGG363200.019.5817742
GTGGTAT79100.019.1165891
ACATGGG366100.019.1111013
GAGTACT345200.018.9521112-13
AGTACTT378500.018.08039712-13
CAGAGTA543700.018.00011810-11
CATGGGG268200.017.8373684
TGGTATC79500.016.9786662
Produced by FastQC (version 0.11.5)